CD4 antibody | b38c6c7
Mouse anti Pig CD4:FITC
- Product Type
- Monoclonal Antibody
- Clone
- b38c6c7
- Isotype
- IgG1
- Specificity
- CD4
Antibody Quality and Performance Guarantee
| Mouse anti Porcine CD4, clone b38c6c7 recognizes a ~55 kDa porcine homologue of the human CD4 antigen found on the surface of T helper cells. Clone b38c6c7 was confirmed to bind to a member of the CD4 alpha cluster at the ‘Third International Workshop on Swine Leukocyte Differentiation Antigens’ (Haverson et al. 2001). Porcine CD4 is a type 1 transmembrane member of the immunoglobulin superfamily. Pigs are unusual among mammalian species as they appear to have four populations of resting T lymphocytes. In addition to CD4+/CD8- and CD4-/CD8+ lymphocytes they also appear to have significant populations of CD4-/CD8- and CD4+/CD8+ cells. Lymphoblasts with a double positive phenotype have been described in other species but not in the case of mature peripheral T cells (Saalmuller et al. 1987). |
- Target Species
- Pig
- Product Form
- Purified IgG conjugated to Fluorescein Isothiocyanate Isomer 1 (FITC) - liquid
- Preparation
- Purified IgG prepared by affinity chromatography on Protein A from tissue culture supernatant
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
- 0.09% Sodium Azide (NaN3)
1% Bovine Serum Albumin - Immunogen
- Porcine leukocytes
- Approx. Protein Concentrations
- IgG concentration 0.1 mg/ml
- Max Ex/Em
-
Fluorophore Excitation Max (nm) Emission Max (nm) FITC 490 525 - Regulatory
- For research purposes only
- Guarantee
- 12 months from date of despatch
This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
| Application Name | Verified | Min Dilution | Max Dilution |
|---|---|---|---|
| Flow Cytometry |  |
Neat | |
| Immunofluorescence | |
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
- Flow Cytometry
- Use 10ul of the suggested working dilution to label 106 cells in 100ul
| Description | Product Code | Applications | Pack Size | List Price | Your Price | Quantity | |
|---|---|---|---|---|---|---|---|
| Mouse IgG1 Negative Control:FITC | MCA928F | F | 100 Tests |
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| Description | Mouse IgG1 Negative Control:FITC | ||||||
| Description | Product Code | Applications | Pack Size | List Price | Your Price | Quantity | |
|---|---|---|---|---|---|---|---|
| Mouse anti Pig CD45:Alexa Fluor® 647 | MCA1222A647 | F | 100 Tests/1ml |
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| Description | Mouse anti Pig CD45:Alexa Fluor® 647 | ||||||
| Mouse anti Pig wCD8 Alpha:RPE | MCA1223PE | F | 100 Tests | Log in | |||
| List Price | Your Price | ||||||
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| Description | Mouse anti Pig wCD8 Alpha:RPE | ||||||
| Mouse anti Pig CD3:RPE | MCA5951PE | F | 100 Tests | Log in | |||
| List Price | Your Price | ||||||
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| Description | Mouse anti Pig CD3:RPE | ||||||
References for CD4 antibody
-
Saalmüller A et al. (2001) Summary of workshop findings for porcine T-lymphocyte-specific monoclonal antibodies.
Vet Immunol Immunopathol. 80 (1-2): 35-52. -
Gabner, S.,et al. (2014) Immune response to Cystoisospora suis in piglets: local and systemic changes in T-cell subsets and selected mRNA transcripts in the small intestine.
Parasite Immunology 36: 277–291
MCA6045F
153049
159426
1803
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