Bcl-2 antibody | 100
Mouse anti Human Bcl-2, clone 100 reacts with small B lymphocytes in the mantle zone and many cells within T cell areas. Very few cells in germinal centres are stained. In the thymus many cells in the medulla are stained but the cortex shows weak or negative staining. In non-haematopoietic tissues few cells are stained (Krajewski et al. 1995). Mouse anti Human Bcl-2 antibody, clone 100 reacts with neoplastic cells of follicular lymphoma and anaplastic large cell lymphoma (Reed 1997).
Mouse anti human Bcl-2, clone 100 has been successfully used to demonstrate Bcl-2 in formalin fixed, paraffin embedded tissues of radioresistant squamous cell carcinoma by immunohistochemistry (Condon et al. 2002).
- Target Species
- Product Form
- Purified IgG - liquid
- Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
0.09% Sodium Azide
- Carrier Free
- Synthetic peptide, amino acids 41-54 of the Bcl-2 protein
- Approx. Protein Concentrations
- IgG concentration 1.0 mg/ml
- This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.
- 12 months from date of despatch
- Entrez Gene
- GO Terms
- GO:0000209 protein polyubiquitination
- GO:0001836 release of cytochrome c from mitochondria
- GO:0002020 protease binding
- GO:0016021 integral to membrane
- GO:0005741 mitochondrial outer membrane
- GO:0005789 endoplasmic reticulum membrane
- GO:0006916 anti-apoptosis
- GO:0006959 humoral immune response
- GO:0006974 response to DNA damage stimulus
- GO:0007565 female pregnancy
- GO:0008629 induction of apoptosis by intracellular signals
- GO:0008633 activation of pro-apoptotic gene products
- GO:0042493 response to drug
- GO:0009636 response to toxin
- GO:0010039 response to iron ion
- GO:0016563 transcription activator activity
- GO:0030307 positive regulation of cell growth
- GO:0030890 positive regulation of B cell proliferation
- GO:0031965 nuclear membrane
- GO:0032848 negative regulation of cellular pH reduction
- GO:0034097 response to cytokine stimulus
- GO:0035094 response to nicotine
- GO:0042100 B cell proliferation
- GO:0042803 protein homodimerization activity
- GO:0043496 regulation of protein homodimerization activity
- GO:0043497 regulation of protein heterodimerization activity
- GO:0043524 negative regulation of neuron apoptosis
- GO:0043565 sequence-specific DNA binding
- GO:0046902 regulation of mitochondrial membrane permeability
- GO:0046982 protein heterodimerization activity
- GO:0050853 B cell receptor signaling pathway
- GO:0051402 neuron apoptosis
- GO:0051434 BH3 domain binding
- GO:0051607 defense response to virus
- GO:0051881 regulation of mitochondrial membrane potential
- GO:0051902 negative regulation of mitochondrial depolarization
- GO:0070059 apoptosis in response to endoplasmic reticulum stress
- For research purposes only
Applications of Bcl-2 antibody
|Application Name||Verified||Min Dilution||Max Dilution|
|Flow Cytometry 1||1/50||1/100|
|Immunohistology - Frozen||1/20||1/40|
|Immunohistology - Paraffin 2||1/20||1/40|
- 1 Membrane permeabilisation is required for this application. Bio-Rad recommends the use of Leucoperm™ (Product Code BUF09) for this purpose.
- 2 This product requires antigen retrieval using heat treatment prior to staining of paraffin sections.
Sodium citrate buffer pH 6.0 is recommended for this purpose.
- Flow Cytometry
- Use 10ul of the suggested working dilution to label 1x106 cells in 100ul.
- Histology Positive Control Tissue
- Human tonsil
Secondary Antibodies Available
Negative Isotype Controls Available
|Description||Product Code||Applications||Pack Size||List Price||Quantity|
|Mouse IgG1 Negative Control||MCA928||F||100 Tests|
Product Specific References
Pezzella, F. et al. (1990) Expression of the bcl-2 oncogene protein is not specific for the 14;18 chromosomal translocation.
Am J Pathol. 137 (2): 225-32.
References for Bcl-2 antibody
Cleary, M.L. et al. (1986) Cloning and structural analysis of cDNAs for bcl-2 and a hybrid bcl-2/immunoglobulin transcript resulting from the t(14;18) translocation.
Cell. 47: 19-28.
Hockenbery, D. et al. (1990) Bcl-2 is an inner mitochondrial membrane protein that blocks programmed cell death.
Nature. 348 (6299): 334-6.
Chylicki, K. et al. (2000) Characterization of the molecular mechanisms for p53-mediated differentiation.
Cell Growth Differ. 11: 561-71.
Shenker, B.J. ,i>et al. (2001) Induction of apoptosis in human T cells by Actinobacillus actinomycetemcomitans cytolethal distending toxin is a consequence of G2 arrest of the cell cycle.
J Immunol. 167: 435-41.
Iannone, F. et al. (2005) Increased Bcl-2/p53 ratio in human osteoarthritic cartilage: a possible role in regulation of chondrocyte metabolism.
Ann Rheum Dis. 64: 217-21.
Iscache, A.L. et al. (2011) Effects of BCL-2 over-expression on B cells in transgenic rats and rat hybridomas.
Int Immunol. 23 (10): 625-36.
Valgimigli, M. et al. (2003) Serum from patients with acute coronary syndromes displays a proapoptotic effect on human endothelial cells: a possible link to pan-coronary syndromes.
Circulation. 107: 264-70.
Berrieman, H.K. et al. (2005) The expression of Bcl-2 family proteins differs between nonsmall cell lung carcinoma subtypes.
Cancer. 103: 1415-9.
Fimognari, C. et al. (2002) Growth inhibition, cell-cycle arrest and apoptosis in human T-cell leukemia by the isothiocyanate sulforaphane.
Carcinogenesis. 23: 581-6.
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