Bcl-2 antibody | 100

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Mouse anti Human Bcl-2

Product Type
Monoclonal Antibody
Clone
100
Isotype
IgG1
Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
MCA1550 C F* IP P* WB 0.1 mg
Mouse anti Human Bcl-2 antibody, clone 100 recognizes the human Apoptosis regulator Bcl-2 oncoprotein, a 239 amino acid ~25kDa integral single pass membrane protein containing 4 BH motifs which lies within the cell rather than on the cell surface. The protein is localised in the outer mitochondrial membrane and plays a role in the inhibition of apoptosis.

Mouse anti Human Bcl-2, clone 100 reacts with small B lymphocytes in the mantle zone and many cells within T cell areas. Very few cells in germinal centres are stained. In the thymus many cells in the medulla are stained but the cortex shows weak or negative staining. In non-haematopoietic tissues few cells are stained (Krajewski et al. 1995). The antibody reacts with neoplastic cells of follicular lymphoma and anaplastic large cell lymphoma (Reed 1997).

Mouse anti human Bcl-2, clone 100 has been successfully used to demonstrate Bcl-2 in formalin fixed, paraffin embedded tissues of radioresistant squamous cell carcinoma by immunohistochemistry (Condon et al. 2002).

Product Details

Target Species
Human
Product Form
Purified IgG - liquid
Preparation
Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.09%Sodium Azide
Carrier Free
Yes
Immunogen
Synthetic peptide, amino acids 41-54 of the Bcl-2 protein
Approx. Protein Concentrations
IgG concentration 1.0 mg/ml

Storage Information

Storage
Store at +4oC or at -20oC if preferred.

This product should be stored undiluted.

Storage in frost free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Shelf Life
18 months from date of despatch.

More Information

UniProt
P10415 Related reagents
Entrez Gene
BCL2 Related reagents
GO Terms
GO:0000209 protein polyubiquitination
GO:0001836 release of cytochrome c from mitochondria
GO:0002020 protease binding
GO:0016021 integral to membrane
GO:0005741 mitochondrial outer membrane
GO:0005789 endoplasmic reticulum membrane
GO:0006916 anti-apoptosis
GO:0006959 humoral immune response
GO:0006974 response to DNA damage stimulus
GO:0007565 female pregnancy
GO:0008629 induction of apoptosis by intracellular signals
GO:0008633 activation of pro-apoptotic gene products
GO:0042493 response to drug
GO:0009636 response to toxin
GO:0010039 response to iron ion
GO:0016563 transcription activator activity
GO:0030307 positive regulation of cell growth
GO:0030890 positive regulation of B cell proliferation
GO:0031965 nuclear membrane
GO:0032848 negative regulation of cellular pH reduction
GO:0034097 response to cytokine stimulus
GO:0035094 response to nicotine
GO:0042100 B cell proliferation
GO:0042803 protein homodimerization activity
GO:0043496 regulation of protein homodimerization activity
GO:0043497 regulation of protein heterodimerization activity
GO:0043524 negative regulation of neuron apoptosis
GO:0043565 sequence-specific DNA binding
GO:0046902 regulation of mitochondrial membrane permeability
GO:0046982 protein heterodimerization activity
GO:0050853 B cell receptor signaling pathway
GO:0051402 neuron apoptosis
GO:0051434 BH3 domain binding
GO:0051607 defense response to virus
GO:0051881 regulation of mitochondrial membrane potential
GO:0051902 negative regulation of mitochondrial depolarization
GO:0070059 apoptosis in response to endoplasmic reticulum stress
Regulatory
For research purposes only

Applications of Bcl-2 antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Flow Cytometry 1 1/50 1/100
Immunohistology - Frozen 1/20 1/40
Immunohistology - Paraffin 2 1/20 1/40
Immunoprecipitation
Western Blotting
  1. 1 Membrane permeabilisation is required for this application. Bio-Rad recommends the use of Leucoperm (Product Code BUF09) for this purpose.
  2. 2 This product requires antigen retrieval using heat treatment prior to staining of paraffin sections.

    Sodium citrate buffer pH 6.0 is recommended for this purpose.
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
Flow Cytometry
Use 10ul of the suggested working dilution to label 1x106 cells in 100ul.
Histology Positive Control Tissue
Human tonsil

Secondary Antibodies Available

Description Product Code Pack Size Applications List Price Quantity
Human anti Mouse IgG1:HRP HCA036P 0.1 mg E
Goat anti Mouse IgG (H/L):Alk. Phos. (Multi Species Adsorbed) STAR117A 0.5 mg E WB
Goat anti Mouse IgG (H/L):DyLight®488 (Multi Species Adsorbed) STAR117D488GA 0.1 mg F IF
Goat anti Mouse IgG (H/L):DyLight®549 (Multi Species Adsorbed) STAR117D549GA 0.1 mg F IF WB
Goat anti Mouse IgG (H/L):DyLight®649 (Multi Species Adsorbed) STAR117D649GA 0.1 mg F IF
Goat anti Mouse IgG (H/L):DyLight®680 (Multi Species Adsorbed) STAR117D680GA 0.1 mg F WB
Goat anti Mouse IgG (H/L):DyLight®800 (Multi Species Adsorbed) STAR117D800GA 0.1 mg F IF WB
Goat anti Mouse IgG (H/L):FITC (Multi Species Adsorbed) STAR117F 0.5 mg F
Goat anti Mouse IgG (H/L):HRP (Multi Species Adsorbed) STAR117P 0.5 mg E WB
Goat anti Mouse IgG (Fc):FITC STAR120F 1 mg C F
Goat anti Mouse IgG (Fc):HRP STAR120P 1 mg E WB
Rabbit F(ab')2 anti Mouse IgG:RPE STAR12A 1 ml F
Rabbit F(ab')2 anti Mouse IgG:HRP (Human Adsorbed) STAR13B 1 mg C E P RE WB
Goat anti Mouse IgG:FITC (Rat Adsorbed) STAR70 0.5 mg F
Goat anti Mouse IgG:RPE (Rat Adsorbed) STAR76 1 ml F
Goat anti Mouse IgG:HRP (Rat Adsorbed) STAR77 0.5 mg C E P
Goat anti Mouse IgG/A/M:Alk. Phos. STAR87A 1 mg C E WB
Goat anti Mouse IgG/A/M:HRP (Human Adsorbed) STAR87P 1 mg E
Rabbit F(ab')2 anti Mouse IgG:Dylight®800 STAR8D800GA 0.1 mg F IF WB
Rabbit F(ab')2 anti Mouse IgG:FITC STAR9B 1 mg F

Negative Isotype Controls Available

Description Product Code Pack Size Applications List Price Quantity
Mouse IgG1 Negative Control MCA928 100 Tests F

Application Based External Images

Flow Cytometry

Western Blotting

Product Specific References

References for Bcl-2 antibody

  1. Tsujimoto, Y. & Croce, C.M. (1986) Analysis of the structure, transcripts, and protein products of bcl-2, the gene involved in human follicular lymphoma.
    Proc Natl Acad Sci U S A. 83 (14): 5214-8.
  2. Cleary, M.L. et al. (1986) Cloning and structural analysis of cDNAs for bcl-2 and a hybrid bcl-2/immunoglobulin transcript resulting from the t(14;18) translocation.
    Cell. 47: 19-28.
  3. Pezzella, F. et al. (1990) Expression of the bcl-2 oncogene protein is not specific for the 14;18 chromosomal translocation.
    Am J Pathol. 137 (2): 225-32.
  4. Hockenbery, D. et al. (1990) Bcl-2 is an inner mitochondrial membrane protein that blocks programmed cell death.
    Nature. 348 (6299): 334-6.
  5. Iannone, F. et al. (2005) Increased Bcl-2/p53 ratio in human osteoarthritic cartilage: a possible role in regulation of chondrocyte metabolism.
    Ann Rheum Dis. 64: 217-21.
  6. Iscache, A.L. et al. (2011) Effects of BCL-2 over-expression on B cells in transgenic rats and rat hybridomas.
    Int Immunol. 23 (10): 625-36.
  7. Chylicki, K. et al. (2000) Characterization of the molecular mechanisms for p53-mediated differentiation.
    Cell Growth Differ. 11: 561-71.
  8. Berrieman, H.K. et al. (2005) The expression of Bcl-2 family proteins differs between nonsmall cell lung carcinoma subtypes.
    Cancer. 103: 1415-9.
  9. Valgimigli, M. et al. (2003) Serum from patients with acute coronary syndromes displays a proapoptotic effect on human endothelial cells: a possible link to pan-coronary syndromes.
    Circulation. 107: 264-70.
  10. Shenker, B.J. ,i>et al. (2001) Induction of apoptosis in human T cells by Actinobacillus actinomycetemcomitans cytolethal distending toxin is a consequence of G2 arrest of the cell cycle.
    J Immunol. 167: 435-41.
  11. Fimognari, C. et al. (2002) Growth inhibition, cell-cycle arrest and apoptosis in human T-cell leukemia by the isothiocyanate sulforaphane.
    Carcinogenesis. 23: 581-6.