IgG antibody
1 μg and 0.25 μg of antibody was run in lanes 2, 4 and 6 and lanes 3, 5 and 7 respectively. Protein detection was carried out on lanes 2 and 3 using Goat anti Mouse IgG (H/L):HRP (STAR207P) at a dilution of 1/10,000. Detection was carried out on lanes 4 and 5 using Rat anti Mouse Kappa Light Chain:HRP (MCA152P) at a dilution of 1/1,000. Detection was carried out on lanes 6 and 7 using Goat anti Mouse IgG (Fc):HRP (STAR120P) at a dilution of 1/5,000. Visualization was carried out using the Chemidoc™ Touch Imaging System.
Goat anti Mouse IgG (H/L) polyclonal antibody reacts with the heavy chains of mouse IgG, and the light chains common to the majority of mouse immunoglobulins. This antibody has not been cross adsorbed against other mouse immunoglobulins, and has been found to react substantially with antibody light chains common to all mouse antibodies. Therefore, significant cross reactivity to non-IgG primary antibodies may occur. No antibody reactivity was detected against non-immunoglobulin mouse serum proteins. This antibody may cross-react with immunoglobulins from other species.
Product Details
- Target Species
- Mouse
- Product Form
- Purified IgG - lyophilized
- Reconstitution
- Reconstitute with 2.0 ml distilled water
Care should be taken during reconstitution as the protein may appear as a film at the bottom of the vial. Bio-Rad recommend that the vial is gently mixed after reconstitution. - Antiserum Preparation
- Antiserum to mouse IgG (H/L) was raised by repeated immunisation of goats with highly purified antigen. Purified IgG was prepared by affinity chromatography.using antigens coupled to agarose beads.
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
- 15 mg/ml Bovine Serum Albumin
- Approx. Protein Concentrations
- IgG concentration 0.8 mg/ml after reconstitution
Storage Information
- Storage
- Store at +4oC. DO NOT FREEZE.
This product should be stored undiluted. - Shelf Life
- 12 months from date of reconstitution.
More Information
- Regulatory
- For research purposes only
Applications of IgG antibody
| Application Name | Verified | Min Dilution | Max Dilution |
|---|---|---|---|
| Western Blotting | 1/5000 | 1/50,000 |
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
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