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IgG antibody

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Goat anti Mouse IgG Ab (H/L) - HRP thumbnail image 1 Goat anti Mouse IgG Ab (H/L) - HRP thumbnail image 2 Goat anti Mouse IgG Ab (H/L) - HRP thumbnail image 3
Goat anti Mouse IgG Ab (H/L) - HRP gallery image 1
A Mouse monoclonal antibody of the IgG1 isotype was run under reducing conditions on SDS PAGE using the Bio-Rad V3 Western Workflow™ and transferred to a PVDF membrane. Precision Plus Protein™ Prestained Standards were run in lane 1.

1 μg and 0.25 μg of antibody was run in lanes 2, 4 and 6 and lanes 3, 5 and 7 respectively. Protein detection was carried out on lanes 2 and 3 using Goat anti Mouse IgG (H/L):HRP (STAR207P) at a dilution of 1/10,000. Detection was carried out on lanes 4 and 5 using Rat anti Mouse Kappa Light Chain:HRP (MCA152P) at a dilution of 1/1,000. Detection was carried out on lanes 6 and 7 using Goat anti Mouse IgG (Fc):HRP (STAR120P) at a dilution of 1/5,000. Visualization was carried out using the Chemidoc™ Touch Imaging System.
Goat anti Mouse IgG Ab (H/L) - HRP gallery image 2
A Mouse monoclonal antibody of the IgG1 isotype was run under reducing conditions on SDS PAGE using the Bio-Rad V3 system and transferred to a PVDF membrane. Bio-Rad Precision Plus molecular weight marker was run in lanes 1 and 5. 1, 0.5 and 0.25 ug of antibody were run in lanes 2, 3 and 4 and 6, 7 and 8 respectively. Protein detection was carried out on lanes 2, 3 and 4 using Goat anti Mouse IgG (H/L):HRP (STAR207P) at a dilution of 1/10,000. Detection was carried out on lanes 6, 7 and 8 using Rat anti Mouse Kappa Light Chain:HRP (MCA152P) at a dilution of 1/1,000. Visualization was carried out using the Bio-Rad ChemiDoc with automatic exposure time. STAR207P is specific for both the heavy (50kDa) and light (25kDa) chains whereas MCA152P is specific for the light chains.
Goat anti Mouse IgG Ab (H/L) - HRP gallery image 3
Detection of recombinant Human CD33 with Mouse anti Human CD33 (MCA1271) in a Western blot analysis with Goat anti Mouse IgG:HRP (STAR207P) at a 1/10000 dilution as the secondary antibody. Different dilutions of MCA1271 are shown on lanes 2, 4, 6 and 8 at 1/200, 1/500, 1/1000 and 1/2000, respectively. Recombinant Human CD33 probed with STAR207P only at a 1/10000 dilution was included as a secondary only control (Lane 10).

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Goat anti Mouse IgG (H/L):HRP

Product Type
Polyclonal Antibody
Isotype
Polyclonal IgG
Specificity
IgG
Region
(H/L)

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Goat anti Mouse IgG (H/L) polyclonal antibody reacts with the heavy chains of mouse IgG, and the light chains common to the majority of mouse immunoglobulins. This antibody has not been cross adsorbed against other mouse immunoglobulins, and has been found to react substantially with antibody light chains common to all mouse antibodies. Therefore, significant cross reactivity to non-IgG primary antibodies may occur. No antibody reactivity was detected against non-immunoglobulin mouse serum proteins. This antibody may cross-react with immunoglobulins from other species.

Target Species
Mouse
Product Form
Purified IgG - lyophilized
Reconstitution
Reconstitute with 2.0 ml distilled water
Care should be taken during reconstitution as the protein may appear as a film at the bottom of the vial. Bio-Rad recommend that the vial is gently mixed after reconstitution.
Antiserum Preparation
Antiserum to mouse IgG (H/L) was raised by repeated immunisation of goats with highly purified antigen. Purified IgG was prepared by affinity chromatography.using antigens coupled to agarose beads.
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
15 mg/ml Bovine Serum Albumin
Approx. Protein Concentrations
IgG concentration 0.8 mg/ml after reconstitution
Regulatory
For research purposes only
Guarantee
12 months from date of despatch

Store at +4oC. DO NOT FREEZE.
This product should be stored undiluted.

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Western Blotting 1/5000 1/50,000
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.

References for IgG antibody

  1. Sadewasser, A. et al. (2019) Anti-Niemann Pick C1 Single-Stranded Oligonucleotides with Locked Nucleic Acids Potently Reduce Ebola Virus Infection In Vitro..
    Mol Ther Nucleic Acids. 16: 686-97.
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Please Note: All Products are "FOR RESEARCH PURPOSES ONLY"

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