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CD4 antibody | CVS4

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Anti Horse CD4 Antibody, clone CVS4 thumbnail image 1 Anti Horse CD4 Antibody, clone CVS4 thumbnail image 2 Anti Horse CD4 Antibody, clone CVS4 thumbnail image 3 Anti Horse CD4 Antibody, clone CVS4 thumbnail image 4 Anti Horse CD4 Antibody, clone CVS4 thumbnail image 5 Anti Horse CD4 Antibody, clone CVS4 thumbnail image 6
Anti Horse CD4 Antibody, clone CVS4 gallery image 1
Horse peripheral blood lymphocytes stained with Mouse anti Horse CD4 (MCA1078GA) followed by Goat anti Mouse IgG:FITC (STAR117F)
Anti Horse CD4 Antibody, clone CVS4 gallery image 2
Horse peripheral blood lymphocytes stained with Mouse anti Horse CD4:FITC (MCA1078F)
Anti Horse CD4 Antibody, clone CVS4 gallery image 3
Horse peripheral blood lymphocytes stained with Mouse anti Horse CD4:RPE (MCA1078PE)
Anti Horse CD4 Antibody, clone CVS4 gallery image 4
Published customer image:
Mouse anti Horse CD4 antibody, clone CVS4 (MCA1078GA) used for evaluation of CD4 expressing white blood cells in equine samples by flow cytometry.
Image caption:
Antigen expression on WBC in CB, and CB-MSC at passage (p) 2, 3 and 5. Consistent gates were applied for all cell analyses. Control sample fluorescence is indicated by black lines, fluorescence of samples incubated with specific antibody by light blue/gray lines. Results were similar for samples from 5 animals. Numbers in each plot represent the mean proportion of cells expressing each antigen.

From: Tessier L, Bienzle D, Williams LB, Koch TG (2015)
Phenotypic and Immunomodulatory Properties of Equine Cord Blood-Derived Mesenchymal Stromal Cells.
PLoS ONE 10(4): e0122954.
This image is from an open access article distributed under the terms of the Creative Commons Attribution License.
Anti Horse CD4 Antibody, clone CVS4 gallery image 5
Figure A. RPE conjugated Mouse anti Horse CD8 (MCA1080PE) and FITC conjugated Mouse IgG2a isotype control (MCA929F). Figure B. RPE conjugated Mouse anti Horse CD8 (MCA1080PE) and FITC conjugated Mouse anti Horse CD4 (MCA1078F). All experiments performed on red cell lysed horse blood gated on lymphocytes in the presence of 10% horse serum. Data acquired on the ZE5™ Cell Analyzer.
Anti Horse CD4 Antibody, clone CVS4 gallery image 6
Figure A. FITC conjugated Mouse anti Horse CD8 (MCA1080F) and RPE conjugated Mouse IgG1 isotype control (MCA928PE). Figure B. FITC conjugated Mouse anti Horse CD8 (MCA1080F) and RPE conjugated Mouse anti Horse CD4 (MCA1078PE). All experiments performed on red cell lysed horse blood gated on lymphocytes in the presence of 10% horse serum. Data acquired on the ZE5™ Cell Analyzer.

Mouse anti Horse CD4:FITC

Mouse anti Horse CD4

Mouse anti Horse CD4:RPE

Product Type
Monoclonal Antibody
Clone
CVS4
Isotype
IgG1
Product Code Applications Pack Size List Price Quantity
MCA1078F
Datasheet
F
MSDS
0.1 mg loader

MCA1078GA
Datasheet
C F IP
MSDS
0.1 mg loader

MCA1078PE
Datasheet
F
MSDS
100 Tests loader

Mouse anti Horse CD4 antibody, clone CVS4 recognizes Equine CD4, a ~58 kDa cell surface glycoprotein that is primarily expressed on a subpopulation of T lymphocytes. As in humans, equine CD4 expression is mutually exclusive with CD8 expression on mature T-cells

A study undertaken using Mouse anti Horse CD4, clone CVS4 to identify CD4 on several wild african equid species indicates that the CVS4 clone recognizes Somali wild ass (Equus asinus) but not Grévy's Zebra (E. grevyi) or Hartmann's Mountain Zebra (E. zebra).

In addition to the CVS4 clone, other CVS clones recognising equine cell surface and MHC antigen are available from Bio-Rad.

Product Details

Target Species
Horse
Product Form
Purified IgG conjugated to Fluorescein Isothiocyanate Isomer 1 (FITC) - liquid
Product Form
Purified IgG - liquid
Product Form
Purified IgG conjugated to R. Phycoerythrin (RPE) - lyophilized
Reconstitution
Reconstitute with 1.0 ml distilled water
Preparation
Purified IgG prepared by affinity chromatography on Protein A from tissue culture supernatant
Preparation
Purified IgG prepared by affinity chromatography on Protein A from tissue culture supernatant
Preparation
Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
Buffer Solution
Phosphate buffered saline
Buffer Solution
Phosphate buffered saline
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.09% Sodium Azide (NaN3)
1% Bovine Serum Albumin
Preservative Stabilisers
0.09% Sodium Azide (NaN3)
Preservative Stabilisers
0.09% Sodium Azide (NaN3)
1% Bovine Serum Albumin
5% Sucrose
Carrier Free
Yes
Immunogen
Equine thymocytes.
Approx. Protein Concentrations
IgG concentration 0.1mg/ml
Approx. Protein Concentrations
IgG concentration 1.0mg/ml
Fusion Partners
Spleen cells from immunised BALB/c mice were fused with cells of the X63-Ag 8.653 mouse myeloma cell line.

Storage Information

Storage
Store at +4oC or at -20oC if preferred.
Storage in frost-free freezers is not recommended.
This product should be stored undiluted. This product is photosensitive and should be protected from light.
Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Storage
Store at +4oC or at -20oC if preferred.

This product should be stored undiluted.

Storage in frost-free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Storage
Prior to reconstitution store at +4oC.
After reconstitution store at +4oC.
DO NOT FREEZE. This product should be stored undiluted. This product is photosensitive and should be protected from light. Should this product contain a precipitate we recommend microcentrifugation before use.
Guarantee
18 months from date of despatch.
Guarantee
18 months from date of despatch.
Guarantee
12 months from date of reconstitution.

More Information

UniProt
F6Y6X8
Regulatory
For research purposes only

Applications of CD4 antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Flow Cytometry Neat 1/10
Flow Cytometry 1/25 1/200
Immunohistology - Frozen
Immunoprecipitation
Flow Cytometry Neat
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
Flow Cytometry
Use 10ul of the suggested working dilution to label 106 cells in 100ul.
Flow Cytometry
Use 10ul of the suggested working dilution to label 106 cells in 100ul.
Flow Cytometry
Use 10ul of the suggested working dilution to label 106 cells in 100ul.
Copyright © 2020 Bio-Rad Antibodies (formerly AbD Serotec)

Secondary Antibodies Available

Description Product Code Applications Pack Size List Price Quantity
Goat anti Mouse IgG (H/L):Alk. Phos. (Multi Species Adsorbed) STAR117A E WB 0.5 mg loader
Goat anti Mouse IgG (H/L):DyLight®488 (Multi Species Adsorbed) STAR117D488GA F IF 0.1 mg loader
Goat anti Mouse IgG (H/L):DyLight®680 (Multi Species Adsorbed) STAR117D680GA F WB 0.1 mg loader
Goat anti Mouse IgG (H/L):DyLight®800 (Multi Species Adsorbed) STAR117D800GA F IF WB 0.1 mg loader
Goat anti Mouse IgG (H/L):FITC (Multi Species Adsorbed) STAR117F F 0.5 mg loader
Goat anti Mouse IgG (H/L):HRP (Multi Species Adsorbed) STAR117P E WB 0.5 mg loader
Goat anti Mouse IgG (Fc):FITC STAR120F C F 1 mg loader
Goat anti Mouse IgG (Fc):HRP STAR120P E WB 1 mg loader
Rabbit F(ab')2 anti Mouse IgG:RPE STAR12A F 1 ml loader
Rabbit F(ab')2 anti Mouse IgG:HRP (Human Adsorbed) STAR13B C E P RE WB 1 mg loader
Goat anti Mouse IgG:FITC (Rat Adsorbed) STAR70 F 0.5 mg loader
Goat anti Mouse IgG:RPE (Rat Adsorbed) STAR76 F 1 ml loader
Goat anti Mouse IgG:HRP (Rat Adsorbed) STAR77 C E P 0.5 mg loader
Goat anti Mouse IgG/A/M:Alk. Phos. STAR87A C E WB 1 mg loader
Goat anti Mouse IgG/A/M:HRP (Human Adsorbed) STAR87P E 1 mg loader
Rabbit F(ab')2 anti Mouse IgG:Dylight®800 STAR8D800GA F IF WB 0.1 mg loader
Rabbit F(ab')2 anti Mouse IgG:FITC STAR9B F 1 mg loader

Useful Reagents Available

Description Product Code Applications Pack Size List Price Quantity
Mouse anti Horse CD8:RPE MCA2385PE F 100 Tests loader
Mouse anti Horse CD8:FITC MCA2385F F 0.1 mg loader
Mouse anti Horse CD8:RPE MCA2385PE F 100 Tests loader
Mouse anti Horse CD8:FITC MCA2385F F 0.1 mg loader

Application Based External Images

Flow Cytometry

Product Specific References

References for CD4 antibody

  1. Lunn, D.P. et al. (1991) Three monoclonal antibodies identifying antigens on all equine T lymphocytes, and two mutually exclusive T-lymphocyte subsets.
    Immunology. 74 (2): 251-7.
  2. Kydd, J. et al. (1994) Report of the First International Workshop on Equine Leucocyte Antigens, Cambridge, UK, July 1991.
    Vet Immunol Immunopathol. 42 (1): 3-60.
  3. Deeg,C.A. et al. (2004) The uveitogenic potential of retinal S-antigen in horses.
    Invest Ophthalmol Vis Sci. 45: 2286-92
  4. Pearson, W. et al. (2007) Low-dose ginseng (Panax quinquefolium) modulates the course and magnitude of the antibody response to vaccination against equid herpesvirus I in horses.
    Can J Vet Res. 71: 213-7.
  5. Brault, S.A. et al. (2010) The immune response of foals to natural infection with equid herpesvirus-2 and its association with febrile illness.
    Vet Immunol Immunopathol. 137: 136-41.
  6. Goodman, L.B. et al. (2007) A point mutation in a herpesvirus polymerase determines neuropathogenicity.
    PLoS Pathog. 3(11):e160.
  7. Hamza, E.et al. (2012) CD4+CD25+ T cells expressing FoxP3 in Icelandic horses affected with insect bite hypersensitivity.
    Vet Immunol Immunopathol. 148 (1-2): 139-44.
  8. Go, Y.Y. et al. (2010) Complex interactions between the major and minor envelope proteins of equine arteritis virus determine its tropism for equine CD3+ T lymphocytes and CD14+ monocytes.
    J Virol. 84: 4898-911
  9. Lunn, D.P. et al. (1998) Report of the Second Equine Leucocyte Antigen Workshop, Squaw valley, California, July 1995.
    Vet Immunol Immunopathol. 62: 101-143
  10. Ibrahim, S. et al. (2007) Screening of anti-human leukocyte monoclonal antibodies for reactivity with equine leukocytes.
    Vet Immunol Immunopathol. 119 (1-2): 63-80.
  11. Lai SW et al. (2004) Influence of Ganoderma lucidum on blood biochemistry and immunocompetence in horses.
    Am J Chin Med. 32 (6): 931-40.
  12. Ferreira-Dias, G. et al. (2005) Seasonal reproduction in the mare: possible role of plasma leptin, body weight and immune status.
    Domest Anim Endocrinol. 29 (1): 203-13.
  13. Agrícola, R. et al. (2008) Blood lymphocyte subpopulations, neutrophil phagocytosis and proteinogram during late pregnancy and postpartum in mares.
    Reprod Domest Anim. 43 (2): 212-7.
  14. de Bruijn, C.M. et al. (2007) Clinical, histopathological and immunophenotypical findings in five horses with cutaneous malignant lymphoma.
    Res Vet Sci. 83 (1): 63-72.
  15. Roberto Da Costa, R.P. et al. (2003) Peripheral blood neutrophil function and lymphocyte subpopulations in cycling mares.
    Reprod Domest Anim. 38 (6): 464-9.
  16. Uner, A. G. et al. (2013) Blood Levels of Selected Metabolic Factors, Cytokines, and Lymphocyte Subpopulations in Arabian and Thoroughbred Horses During the Longest and Shortest Days of the Year
    J Equine Vet Sci. 33 (11): 969-976.
  17. Garcia-Tapia, D. et al. (2006) Replication of West Nile virus in equine peripheral blood mononuclear cells.
    Vet Immunol Immunopathol. 110 (3-4): 229-44.
  18. Tessier, L. et al. (2015) Phenotypic and immunomodulatory properties of equine cord blood-derived mesenchymal stromal cells.
    PLoS One. 10 (4): e0122954.
  19. Khol-Parisini, A. et al. (2012) Highly deoxynivalenol contaminated oats and immune function in horses.
    Arch Anim Nutr. 66 (2): 149-61.
  20. Ziegler, A. et al. (2016) Identification and characterization of equine blood plasmacytoid dendritic cells.
    Dev Comp Immunol. 65: 352-7.
  21. Behrens, N.E. & Gershwin, L.J. (2015) Immune modulation of T regulatory cells and IgE responses in horses vaccinated with West Nile virus vaccine combined with a CpG ODN.
    Vaccine. 33 (43): 5764-71.
  22. Degroote, R.L. et al. (2017) Formin like 1 expression is increased on CD4+ T lymphocytes in spontaneous autoimmune uveitis.
    J Proteomics. 154: 102-108.
  23. Krakowski. L. et al. (2017) Changes in Blood Lymphocyte Subpopulations and Expression of MHC-II Molecules in Wild Mares Before and After Parturition.
    J Vet Res. 61 (2): 217-21.
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