CD4 antibody | CVS4
Mouse anti Horse CD4 antibody, clone CVS4 used for evaluation of CD4 expressing white blood cells in equine samples by flow cytometry.
Image caption:
Antigen expression on WBC in CB, and CB-MSC at passage (p) 2, 3 and 5. Consistent gates were applied for all cell analyses. Control sample fluorescence is indicated by black lines, fluorescence of samples incubated with specific antibody by light blue/gray lines. Results were similar for samples from 5 animals. Numbers in each plot represent the mean proportion of cells expressing each antigen.
From: Tessier L, Bienzle D, Williams LB, Koch TG (2015) Phenotypic and Immunomodulatory Properties of Equine Cord Blood-Derived Mesenchymal Stromal Cells. PLoS ONE 10(4): e0122954.
Mouse anti Horse CD4 antibody, clone CVS4 recognizes Equine CD4, a ~58 kDa cell surface glycoprotein that is primarily expressed on a subpopulation of T lymphocytes. As in humans, equine CD4 expression is mutually exclusive with CD8 expression on mature T-cells
A study undertaken using Mouse anti Horse CD4, clone CVS4 to identify CD4 on several wild african equid species indicates that the CVS4 clone recognizes Somali wild ass (Equus asinus) but not Grévy's Zebra (E. grevyi) or Hartmann's Mountain Zebra (E. zebra).
In addition to the CVS4 clone, other CVS clones recognising equine cell surface and MHC antigen are available from Bio-Rad.
A study undertaken using Mouse anti Horse CD4, clone CVS4 to identify CD4 on several wild african equid species indicates that the CVS4 clone recognizes Somali wild ass (Equus asinus) but not Grévy's Zebra (E. grevyi) or Hartmann's Mountain Zebra (E. zebra).
In addition to the CVS4 clone, other CVS clones recognising equine cell surface and MHC antigen are available from Bio-Rad.
Product Details
- Target Species
- Horse
- Product Form
- Purified IgG conjugated to Fluorescein Isothiocyanate Isomer 1 (FITC) - liquid
- Product Form
- Purified IgG - liquid
- Product Form
- Purified IgG conjugated to R. Phycoerythrin (RPE) - lyophilized
- Reconstitution
- Reconstitute with 1.0 ml distilled water
- Preparation
- Purified IgG prepared by affinity chromatography on Protein A from tissue culture supernatant
- Preparation
- Purified IgG prepared by affinity chromatography on Protein A from tissue culture supernatant
- Preparation
- Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
- 0.09% Sodium Azide (NaN3)
1% Bovine Serum Albumin - Preservative Stabilisers
- 0.09% Sodium Azide (NaN3)
- Preservative Stabilisers
- 0.09% Sodium Azide (NaN3)
1% Bovine Serum Albumin
5% Sucrose - Carrier Free
- Yes
- Immunogen
- Equine thymocytes.
- Approx. Protein Concentrations
- IgG concentration 0.1mg/ml
- Approx. Protein Concentrations
- IgG concentration 1.0mg/ml
- Fusion Partners
- Spleen cells from immunised BALB/c mice were fused with cells of the X63-Ag 8.653 mouse myeloma cell line.
Storage Information
- Storage
- Store at +4oC or at -20oC if preferred.
Storage in frost-free freezers is not recommended.
This product should be stored undiluted. This product is photosensitive and should be protected from light.
Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use. - Storage
- Store at +4oC or at -20oC if preferred.
This product should be stored undiluted.
Storage in frost-free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use. - Storage
- Prior to reconstitution store at +4oC.
After reconstitution store at +4oC.
DO NOT FREEZE. This product should be stored undiluted. This product is photosensitive and should be protected from light. Should this product contain a precipitate we recommend microcentrifugation before use. - Shelf Life
- 18 months from date of despatch.
- Shelf Life
- 18 months from date of despatch.
- Shelf Life
- 12 months from date of reconstitution.
More Information
- UniProt
- F6Y6X8 Related reagents
- Regulatory
- For research purposes only
Applications of CD4 antibody
| Application Name | Verified | Min Dilution | Max Dilution |
|---|---|---|---|
| Flow Cytometry | Neat | 1/10 | |
| Flow Cytometry | 1/25 | 1/200 | |
| Immunohistology - Frozen | |||
| Immunoprecipitation | |||
| Flow Cytometry | Neat |
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
- Flow Cytometry
- Use 10ul of the suggested working dilution to label 106 cells in 100ul.
- Flow Cytometry
- Use 10ul of the suggested working dilution to label 106 cells in 100ul.
- Flow Cytometry
- Use 10ul of the suggested working dilution to label 106 cells in 100ul.
Copyright © 2018 Bio-Rad Antibodies (formerly AbD Serotec)
Secondary Antibodies Available
Useful Reagents Available
| Description | Product Code | Pack Size | Applications | List Price | Quantity |
|---|---|---|---|---|---|
| Mouse anti Horse CD8:RPE | MCA2385PE | 100 Tests | F | ||
| Mouse anti Horse CD8:FITC | MCA2385F | 0.1 mg | F | ||
| Mouse anti Horse CD8:RPE | MCA2385PE | 100 Tests | F | ||
| Mouse anti Horse CD8:FITC | MCA2385F | 0.1 mg | F |
Product Specific References
References for CD4 antibody
-
Lunn, D.P. et al. (1991) Three monoclonal antibodies identifying antigens on all equine T lymphocytes, and two mutually exclusive T-lymphocyte subsets.
Immunology. 74 (2): 251-7. -
Kydd, J. et al. (1994) Report of the First International Workshop on Equine Leucocyte Antigens, Cambridge, UK, July 1991.
Vet Immunol Immunopathol. 42 (1): 3-60. -
Deeg,C.A. et al. (2004) The uveitogenic potential of retinal S-antigen in horses.
Invest Ophthalmol Vis Sci. 45: 2286-92 -
Pearson, W. et al. (2007) Low-dose ginseng (Panax quinquefolium) modulates the course and magnitude of the antibody response to vaccination against equid herpesvirus I in horses.
Can J Vet Res. 71: 213-7. -
Brault, S.A. et al. (2010) The immune response of foals to natural infection with equid herpesvirus-2 and its association with febrile illness.
Vet Immunol Immunopathol. 137: 136-41. -
Goodman, L.B. et al. (2007) A point mutation in a herpesvirus polymerase determines neuropathogenicity.
PLoS Pathog. 3(11):e160. -
Hamza, E.et al. (2012) CD4+CD25+ T cells expressing FoxP3 in Icelandic horses affected with insect bite hypersensitivity.
Vet Immunol Immunopathol. 148 (1-2): 139-44. -
Go, Y.Y. et al. (2010) Complex interactions between the major and minor envelope proteins of equine arteritis virus determine its tropism for equine CD3+ T lymphocytes and CD14+ monocytes.
J Virol. 84: 4898-911 -
Lunn, D.P. et al. (1998) Report of the Second Equine Leucocyte Antigen Workshop, Squaw valley, California, July 1995.
Vet Immunol Immunopathol. 62: 101-143 -
Ibrahim, S. (2007) Analysis of monoclonal antibody cross-reactivity with leukocytes from equids and cloning of CD28.
Anchor Text -
Lai SW et al. (2004) Influence of Ganoderma lucidum on blood biochemistry and immunocompetence in horses.
Am J Chin Med. 32 (6): 931-40. -
Ferreira-Dias, G. et al. (2005) Seasonal reproduction in the mare: possible role of plasma leptin, body weight and immune status.
Domest Anim Endocrinol. 29 (1): 203-13. -
Agrícola, R. et al. (2008) Blood lymphocyte subpopulations, neutrophil phagocytosis and proteinogram during late pregnancy and postpartum in mares.
Reprod Domest Anim. 43 (2): 212-7. -
de Bruijn, C.M. et al. (2007) Clinical, histopathological and immunophenotypical findings in five horses with cutaneous malignant lymphoma.
Res Vet Sci. 83 (1): 63-72. -
Roberto Da Costa, R.P. et al. (2003) Peripheral blood neutrophil function and lymphocyte subpopulations in cycling mares.
Reprod Domest Anim. 38 (6): 464-9. -
Uner, A. G. et al. (2013) Blood Levels of Selected Metabolic Factors, Cytokines, and Lymphocyte Subpopulations in Arabian and Thoroughbred Horses During the Longest and Shortest Days of the Year
J Equine Vet Sci. 33 (11): 969-976. -
Garcia-Tapia, D. et al. (2006) Replication of West Nile virus in equine peripheral blood mononuclear cells.
Vet Immunol Immunopathol. 110 (3-4): 229-44. -
Tessier, L. et al. (2015) Phenotypic and immunomodulatory properties of equine cord blood-derived mesenchymal stromal cells.
PLoS One. 10 (4): e0122954. -
Khol-Parisini, A. et al. (2012) Highly deoxynivalenol contaminated oats and immune function in horses.
Arch Anim Nutr. 66 (2): 149-61. -
Ziegler, A. et al. (2016) Identification and characterization of equine blood plasmacytoid dendritic cells.
Dev Comp Immunol. 65: 352-7. -
Behrens, N.E. & Gershwin, L.J. (2015) Immune modulation of T regulatory cells and IgE responses in horses vaccinated with West Nile virus vaccine combined with a CpG ODN.
Vaccine. 33 (43): 5764-71. -
Degroote, R.L. et al. (2017) Formin like 1 expression is increased on CD4+ T lymphocytes in spontaneous autoimmune uveitis.
J Proteomics. 154: 102-108. -
Krakowski. L. et al. (2017) Changes in blood lymphocyte subpopulations and expression of MHC-II molecules in wild mares before and after parturition
J Vet Res. 61 (2): 217-21.
