CD8 antibody | CVS8
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|Mouse anti Horse CD8 (clone CVS8), is a monoclonal antibody recognising the equine homologue of the human CD8 cell surface antigen which is expressed by a subset of T lymphocytes.
A study undertaken using CVS8 to identify CD8 on several wild african equid species indicates that the CVS8 clone recognizes Somali wild ass (Equus asinus) but not Grévy's Zebra (E. grevyi) or Hartmann's Mountain Zebra (E. zebra) (Ibrahim 2007).
- Target Species
- Species Cross-Reactivity
Target Species Cross Reactivity Ass Zebra
- N.B. Antibody reactivity and working conditions may vary between species.
- Product Form
- Purified IgG conjugated to Fluorescein Isothiocyanate Isomer 1 (FITC) - liquid
- Purified IgG prepared by affinity chromatography on Protein A from tissue culture supernatant
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
- 0.09% Sodium Azide (NaN3)
1% Bovine Serum Albumin
- Equine PBMCs.
- Approx. Protein Concentrations
- IgG concentration 0.1 mg/ml
- Fusion Partners
- Spleen cells from immunized BALB/c mice were fused with cells of the X63-Ag8.653 myeloma cell line.
- Max Ex/Em
Fluorophore Excitation Max (nm) Emission Max (nm) FITC 490 525
- For research purposes only
- 12 months from date of despatch
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended. This product is photosensitive and should be protected from light.
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- Flow Cytometry
- Use 10ul of the suggested working dilution to label 106 cells in 100ul.
References for CD8 antibody
Lunn, D.P et al (1991) Three monoclonal antibodies identifying antigens on all equine T lymphocytes, and two mutually exclusive T-lymphocyte subsets
Immunology 74: 251-257.
(2007) Experimental infection of neonatal foals with Rhodococcus equi triggers adult-like gamma interferon induction.
Clin Vaccine Immunol. 14: 669-77.
Pearson, W. et al. (2007) Low-dose ginseng (Panax quinquefolium) modulates the course and magnitude of the antibody response to vaccination against equid herpesvirus I in horses.
Can J Vet Res. 71: 213-7.
Lunn, D.P. et al. (1998) Report of the second equine leucocyte antigen workshop, Squaw Valley, California July 1995.
Vet Immunol Immunopathol. 62: 101-143.
Merant, C. et al. (2003) Cross-species reactivity of seven monoclonal antibodies with equine lymphocytes by flow cytometry.
Vet Res. 34: 791-801.
Ibrahim, S (2007) Analysis of monoclonal antibody cross-reactivity with leukocytes from equids and cloning of CD28
Chapter 5 in PhD Thesis Freie Universität Berlin
Carossino, M. et al. (2019) Equine arteritis virus long-term persistence is orchestrated by CD8+ T lymphocyte transcription factors, inhibitory receptors, and the CXCL16/CXCR6 axis.
PLoS Pathog. 15 (7): e1007950.
Tomlinson, J.E. et al. (2018) Multispectral fluorescence-activated cell sorting of B and T cell subpopulations from equine peripheral blood.
Vet Immunol Immunopathol. 199: 22-31.
View The Latest Product References
Placci, M. et al. (2020) Natural Horse Boarding Vs Traditional Stable: A Comparison of Hormonal, Hematological and Immunological Parameters.
J Appl Anim Welf Sci. 23 (3): 366-77.
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