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CD8 antibody | CVS21

Mouse anti Horse CD8:FITC

Product Type
Monoclonal Antibody
Clone
CVS21
Isotype
IgG2a
Specificity
CD8

Product Code Applications Pack Size List Price Your Price Qty
MCA1080F
Datasheet Datasheet Datasheet
SDS Safety Datasheet SDS
F 0.1 mg loader
List Price Your Price
loader

Mouse anti Horse CD8 antibody, clone CVS21 recognizes the equine homolog of human CD8. Equine CD8 is expressed by a subset of T lymphocytes.

In addition to the CVS21 clone, other CVS clones recognising equine MHC and cell surface antigens are available.

Target Species
Horse
Product Form
Purified IgG conjugated to Fluorescein Isothiocyanate Isomer 1 (FITC) - liquid
Preparation
Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.09% sodium azide (NaN3)
1% bovine serum albumin
Immunogen
Equine peripheral blood mononuclear cells.
Approx. Protein Concentrations
IgG concentration 0.1 mg/ml
Fusion Partners
Spleen cells from immunized mice were fused with cells of the X63-Ag 8.653 mouse myeloma cell line.
Max Ex/Em
Fluorophore Excitation Max (nm) Emission Max (nm)
FITC 490 525
Regulatory
For research purposes only
Guarantee
12 months from date of despatch

This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.

Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended. This product is photosensitive and should be protected from light.

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Flow Cytometry Neat 1/10
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
Flow Cytometry
Use 10μl of the suggested working dilution to label 106 cells in 100μl

Description Product Code Applications Pack Size List Price Your Price Quantity
Mouse anti Horse CD4:RPE MCA1078PE F 100 Tests
List Price Your Price
Description Mouse anti Horse CD4:RPE

References for CD8 antibody

  1. Ferreira-Dias, G. et al. (2005) Seasonal reproduction in the mare: possible role of plasma leptin, body weight and immune status.
    Domest Anim Endocrinol. 29: 203-13.
  2. Krakowski, L. et al. (2017) Changes in blood lymphocyte subpopulations and expression of MHC-II molecules in wild mares before and after parturition
    J Vet Res. 61 (2): 217-21.
  3. Schauer, M. et al. (2018) Interaction of septin 7 and DOCK8 in equine lymphocytes reveals novel insights into signaling pathways associated with autoimmunity.
    Sci Rep. 8 (1): 12332.
  4. Tomlinson, J.E. et al. (2018) Multispectral fluorescence-activated cell sorting of B and T cell subpopulations from equine peripheral blood.
    Vet Immunol Immunopathol. 199: 22-31.
  5. Hillmann, A. et al. (2019) A novel direct co-culture assay analyzed by multicolor flow cytometry reveals context- and cell type-specific immunomodulatory effects of equine mesenchymal stromal cells.
    PLoS One. 14 (6): e0218949.
  6. Witonsky, S. et al. (2019) Can levamisole upregulate the equine cell-mediated macrophage (M1) dendritic cell (DC1) T-helper 1 (CD4 Th1) T-cytotoxic (CD8) immune response in vitro.?
    J Vet Intern Med. 33 (2): 889-96.
  7. Lucassen, A. et al. (2021) A Saccharomyces cerevisiae Fermentation Product (Olimond BB) Alters the Early Response after Influenza Vaccination in Racehorses.
    Animals (Basel). 11(9):2726.
  8. Townsend, K.S. et al. (2023) Concurrent chronic lymphocytic leukemia and primary hyperparathyroidism in a mule.
    J Vet Intern Med. 37 (3): 1250-5.
  9. View The Latest Product References

MCA1080F

166542 167019 1804

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Please Note: All Products are "FOR RESEARCH PURPOSES ONLY"

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