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IgA antibody | K117 1A8

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Anti Dog IgA Antibody, clone K117 1A8 gallery image 1
Purified Canine IgA deteced with Mouse anti Dog IgA (MCA631GA) followed by Goat anti Mouse IgG (H/L):HRP (STAR117P).

Mouse anti Dog IgA

Product Type
Monoclonal Antibody
Clone
K117 1A8
Isotype
IgG2b
Product Code Applications Pack Size List Price Quantity
MCA631GA
Datasheet
E
MSDS
0.1 mg loader

Mouse anti Dog IgA antibody, clone K117 1A8 recognizes canine IgA and does not cross react with canine IgG or IgM.

Product Details

Target Species
Dog
Species Cross-Reactivity
Target SpeciesCross Reactivity
Wolf
N.B. Antibody reactivity and working conditions may vary between species.
Product Form
Purified IgG - liquid
Preparation
Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.09% Sodium Azide (NaN3)
Carrier Free
Yes
Immunogen
Canine IgA myeloma protein
Approx. Protein Concentrations
IgG concentration 1.0 mg/ml
Fusion Partners
Spleen cells from immunized mice were fused with cells of the mouse P3-X63-Ag8.653 myeloma cell line

Storage Information

Storage
Store at +4oC or at -20oC if preferred.

This product should be stored undiluted.

Storage in frost free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Guarantee
18 months from date of despatch

More Information

Regulatory
For research purposes only

Applications of IgA antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
ELISA 1/20,000 1/300,000
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
ELISA
This product is suitable for use in direct ELISA applications.
Copyright © 2020 Bio-Rad Antibodies (formerly AbD Serotec)

Secondary Antibodies Available

Description Product Code Applications Pack Size List Price Quantity
Human anti Mouse IgG2b:FITC HCA038F F 0.1 mg loader
Goat anti Mouse IgG (H/L):Alk. Phos. (Multi Species Adsorbed) STAR117A E WB 0.5 mg loader
Goat anti Mouse IgG (H/L):DyLight®488 (Multi Species Adsorbed) STAR117D488GA F IF 0.1 mg loader
Goat anti Mouse IgG (H/L):DyLight®680 (Multi Species Adsorbed) STAR117D680GA F WB 0.1 mg loader
Goat anti Mouse IgG (H/L):DyLight®800 (Multi Species Adsorbed) STAR117D800GA F IF WB 0.1 mg loader
Goat anti Mouse IgG (H/L):FITC (Multi Species Adsorbed) STAR117F F 0.5 mg loader
Goat anti Mouse IgG (H/L):HRP (Multi Species Adsorbed) STAR117P E WB 0.5 mg loader
Goat anti Mouse IgG (Fc):FITC STAR120F C F 1 mg loader
Goat anti Mouse IgG (Fc):HRP STAR120P E WB 1 mg loader
Rabbit F(ab')2 anti Mouse IgG:RPE STAR12A F 1 ml loader
Rabbit F(ab')2 anti Mouse IgG:HRP (Human Adsorbed) STAR13B C E P RE WB 1 mg loader
Goat anti Mouse IgG:FITC (Rat Adsorbed) STAR70 F 0.5 mg loader
Goat anti Mouse IgG:RPE (Rat Adsorbed) STAR76 F 1 ml loader
Goat anti Mouse IgG:HRP (Rat Adsorbed) STAR77 C E P 0.5 mg loader
Goat anti Mouse IgG/A/M:Alk. Phos. STAR87A C E WB 1 mg loader
Goat anti Mouse IgG/A/M:HRP (Human Adsorbed) STAR87P E 1 mg loader
Rabbit F(ab')2 anti Mouse IgG:Dylight®800 STAR8D800GA F IF WB 0.1 mg loader
Rabbit F(ab')2 anti Mouse IgG:FITC STAR9B F 1 mg loader

Useful Reagents Available

Description Product Code Applications Pack Size List Price Quantity
Mouse anti Dog IgE MCA1895 E WB 0.25 mg loader
Mouse anti Dog IgE MCA1896 E WB 0.25 mg loader
Mouse anti Dog IgE:Biotin MCA1896B E WB 0.25 mg loader
Mouse anti Dog IgG2 MCA2328GA E F WB 0.1 mg loader

Application Based External Images

Western Blotting

Product Specific References

References for IgA antibody

  1. Simpson, K.W. et al. (2009) Influence of Enterococcus faecium SF68 probiotic on giardiasis in dogs.
    J Vet Intern Med. 23: 476-81.
  2. Peters, I.R. et al. (2004) Measurement of immunoglobulin concentrations in the feces of healthy dogs.
    Clin Diagn Lab Immunol. 11: 841-8.
  3. Frankowiack, M. et al. (2013) IgA deficiency in wolves.
    Dev Comp Immunol. pii: S0145-305X(13)00019-0.
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