IgA antibody | K117 1A8
Mouse anti Dog IgA antibody, clone K117 1A8 (MCA631GA) used as a capture reagent in a sandwich ELISA with HRP conjugated anti canine IgA (AAI31P) as a detection reagent.
Image caption:
Immunoglobulin IgA titers in fecal contents collected at the beginning of the study (T0), after 2 weeks (T2), 4 weeks (T4), 6 weeks (T6), and 8 weeks (T8) in dogs fed the same diet with (Group A) or without Slab51® probiotic supplement (Group B). Black hashtags indicate statistical significance between groups. Red asterisks indicate statistical significance by study times within group A.
From: Rossi G, Pengo G, Galosi L, Berardi S, Tambella AM, Attili AR, Gavazza A, Cerquetella M, Jergens AE, Guard BC, Lidbury JA, Stainer JM, Crovace AM and Suchodolski JS (2020)
Effects of the Probiotic Mixture Slab51® (SivoMixx®) as Food Supplement in Healthy Dogs: Evaluation of Fecal Microbiota, Clinical Parameters and Immune Function.
Front. Vet. Sci. 7:613.
doi: 10.3389/fvets.2020.00613
This image is from an open access article distributed under terms of a Creative Commons Attribution License.
Mouse anti Canine IgA antibody, clone K117 1A8 (MCA631GA) used as a capture reagent in a sandwich ELISA with HRP conjugated Goat anti canine IgA antibody (AAI31P) used as a detection reagent.
Image caption:
GALT response: Changes in fecal IgA in each group during the trial are depicted as mean fecal IgA values ± SEM. X-axis depicts time in weeks and Y-axis depicts mean fecal IgA values in μg/ml. #p <0.05.
From: Satyaraj E, Reynolds A, Engler R, Labuda J, Sun P.
Supplementation of Diets With Spirulina Influences Immune and Gut Function in Dogs.
Front Nutr. 2021 May 28;8:667072.
doi: 10.3389/fnut.2021.667072.
This image is from an open access article distributed under terms of a Creative Commons Attribution License.
Mouse anti Dog IgA antibody, clone K117 1A8 recognizes canine IgA and does not cross react with canine IgG or IgM.
Product Details
- Target Species
- Dog
- Species Cross-Reactivity
-
Target Species Cross Reactivity Wolf - N.B. Antibody reactivity and working conditions may vary between species.
- Product Form
- Purified IgG - liquid
- Preparation
- Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
- 0.09% Sodium Azide (NaN3)
- Carrier Free
- Yes
- Immunogen
- Canine IgA myeloma protein
- Approx. Protein Concentrations
- IgG concentration 1.0 mg/ml
- Fusion Partners
- Spleen cells from immunized mice were fused with cells of the mouse P3-X63-Ag8.653 myeloma cell line
Storage Information
- Storage
- This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended. - Guarantee
- 12 months from date of despatch
More Information
- Regulatory
- For research purposes only
Applications of IgA antibody
| Application Name | Verified | Min Dilution | Max Dilution |
|---|---|---|---|
| ELISA | 1/20,000 | 1/300,000 |
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
- ELISA
- This product is suitable for use in direct ELISA applications.
Copyright © 2022 Bio-Rad Antibodies (formerly AbD Serotec)
Secondary Antibodies Available
Useful Reagents Available
| Description | Product Code | Applications | Pack Size | List Price | Quantity |
|---|---|---|---|---|---|
| Mouse anti Dog IgE | MCA1895 | E WB | 0.25 mg |
|
|
| Mouse anti Dog IgE | MCA1896 | E WB | 0.25 mg |
|
|
| Mouse anti Dog IgE:Biotin | MCA1896B | E WB | 0.25 mg |
|
|
| Mouse anti Dog IgG2 | MCA2328GA | E F WB | 0.1 mg |
|
Product Specific References
References for IgA antibody
-
Simpson, K.W. et al. (2009) Influence of Enterococcus faecium SF68 probiotic on giardiasis in dogs.
J Vet Intern Med. 23: 476-81. -
Peters, I.R. et al. (2004) Measurement of immunoglobulin concentrations in the feces of healthy dogs.
Clin Diagn Lab Immunol. 11: 841-8. -
Frankowiack, M. et al. (2013) IgA deficiency in wolves.
Dev Comp Immunol. pii: S0145-305X(13)00019-0. -
Rossi, G. et al. (2020) Effects of the Probiotic Mixture Slab51® (SivoMixx®) as Food Supplement in Healthy Dogs: Evaluation of Fecal Microbiota, Clinical Parameters and Immune Function
Frontiers in Veterinary Science. 04 Sep [Epub ahead of print]. -
Satyaraj, E. et al. (2021) Supplementation of Diets With Spirulina Influences Immune and Gut Function in Dogs.
Front Nutr. 8: 667072.
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