IgE antibody | E6-2A1

Mouse anti Dog IgE

Product Type
Monoclonal Antibody
Clone
E6-2A1
Isotype
IgG2a
Specificity
IgE

Product Code Applications Pack Size List Price Your Price Qty
MCA1895
Datasheet Datasheet Datasheet
SDS Safety Datasheet SDS
E WB 0.25 mg loader
List Price Your Price
loader
Search for Batch Specific Datasheets

Mouse anti Dog IgE antibody, clone E6-2A1 recognises canine IgE. E6-2A1 does not cross react with dog IgM, IgA, IgG1 or IgG2. Western blot analysis against affinity purified dog IgE using Mouse anti Dog IgE clone E6-2A1 demonstrates a single major band of ~62-65 kDa under reducing conditions.

Target Species
Dog
Product Form
Purified IgG - liquid
Preparation
Purified IgG prepared by affinity chromatography on Protein A from tissue culture supernatant
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
<0.1% sodium azide (NaN3)
Immunogen
Affinity purified IgE preparation from heavily parasitized and allergic dog serum.
Approx. Protein Concentrations
IgG concentration 1.0 mg/ml
Regulatory
For research purposes only
Guarantee
12 months from date of despatch

This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.

Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
ELISA
Western Blotting
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
ELISA
Mouse anti Dog IgE antibody, clone E6-2A1 may be used as a capture antibody in a sandwich ELISA in combination with MCA1896B as the detection antibody.

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Description Goat anti Mouse IgG:RPE (Rat Adsorbed)
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Description Goat anti Mouse IgG/A/M:HRP (Human Adsorbed)
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Description Rabbit F(ab')2 anti Mouse IgG:HRP (Human Adsorbed)
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Description Goat anti Mouse IgG (Fc):FITC
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Description Goat anti Mouse IgG (Fc):HRP
Goat anti Mouse IgG (H/L):HRP (Multi Species Adsorbed) STAR117P C E WB 0.5 mg loader
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Description Goat anti Mouse IgG (H/L):HRP (Multi Species Adsorbed)
Goat anti Mouse IgG (H/L):Alk. Phos. (Multi Species Adsorbed) STAR117A E WB 0.5 mg loader
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Description Goat anti Mouse IgG (H/L):Alk. Phos. (Multi Species Adsorbed)
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Description Goat anti Mouse IgG (H/L):FITC (Multi Species Adsorbed)
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Description Goat anti Mouse IgG (H/L):DyLight®488 (Multi Species Adsorbed)
Goat anti Mouse IgG (H/L):DyLight®680 (Multi Species Adsorbed) STAR117D680GA F WB 0.1 mg loader
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Description Goat anti Mouse IgG (H/L):DyLight®680 (Multi Species Adsorbed)
Goat anti Mouse IgG (H/L):DyLight®800 (Multi Species Adsorbed) STAR117D800GA F IF WB 0.1 mg loader
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Description Goat anti Mouse IgG (H/L):DyLight®800 (Multi Species Adsorbed)
Goat anti Mouse IgG (H/L):DyLight®650 (Multi Species Adsorbed) STAR117D650 F IF 0.1 mg loader
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Description Goat anti Mouse IgG (H/L):DyLight®650 (Multi Species Adsorbed)
Goat anti Mouse IgG (H/L):DyLight®550 (Multi Species Adsorbed) STAR117D550 F IF WB 0.1 mg loader
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Description Goat anti Mouse IgG (H/L):DyLight®550 (Multi Species Adsorbed)

References for IgE antibody

  1. Lin, T.Y. et al. (2006) Generation and characterization of bone marrow-derived cultured canine mast cells.
    Vet Immunol Immunopathol. 113 (1-2): 37-52.
  2. Lin, T.Y. & London, C.A. (2006) A functional comparison of canine and murine bone marrow derived cultured mast cells.
    Vet Immunol Immunopathol. 114 (3-4): 320-34.
  3. Khantavee, N. et al. (2020) Antibody levels to Malassezia pachydermatis. and Staphylococcus pseudintermedius. in atopic dogs and their relationship with lesion scores.
    Vet Dermatol. 31 (2): 111-115.
  4. Khantavee, N. et al. (2021) Immunoglobulin G1 subclass responses can be used to detect specific allergy to the house dust mites Dermatophagoides farinae. and Dermatophagoides pteronyssinus. in atopic dogs.
    BMC Vet Res. 17 (1): 71.

RRID
AB_323084
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Please Note: All Products are "FOR RESEARCH PURPOSES ONLY"

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