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IgE antibody | E6-71A1

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Anti Dog IgE Antibody, clone E6-71A1 thumbnail image 1 Anti Dog IgE Antibody, clone E6-71A1 thumbnail image 2
Anti Dog IgE Antibody, clone E6-71A1 gallery image 1
Published customer image:
Biotin conjugated Mouse anti Canine IgE antibody, clone E6-71-A1 (MCA1896B) used for the detection of canine IgE in sera on two dimensional immunoblots
Image caption:
A – IgE immunoblot from D. glomerata 2-D separation, revealed with biotin-ExtrAvidin-alkaline phosphatase anti-dog-IgE. Dog D. glomerata allergogram with a pool of sera. B – Two-dimensional diagram from dog D. glomerata allergogram in Figure 3 A. Recognized allergen spots are identified from a to n

From: Martins LM, Marques AG, Pereira LM, Semião-Santos SJ, Bento OP.
Allergy to grass pollen: mapping of Dactylis glomerata and Phleum pratense allergens for dogs by two-dimensional immunoblotting.
Postepy Dermatol Alergol. 2017 Feb;34(1):60-9.
Anti Dog IgE Antibody, clone E6-71A1 gallery image 2
Published customer image:
Biotin conjugated Mouse anti Canine IgE antibody, clone E6-71-A1 (MCA1896B) used for the detection of canine IgE in sera on two dimensional immunoblots
Image caption:
A – IgE immunoblot from P. pratense 2-D separation, revealed with biotin-ExtrAvidin-alkaline phosphatase antidog-IgE. Dog P. pratense allergogram with a pool of sera. B – Two-dimensional diagram from dog P. pratense allergogram in Figure 4 A. Recognized allergen spots are identified from a to z7

From: Martins LM, Marques AG, Pereira LM, Semião-Santos SJ, Bento OP.
Allergy to grass pollen: mapping of Dactylis glomerata and Phleum pratense allergens for dogs by two-dimensional immunoblotting.
Postepy Dermatol Alergol. 2017 Feb;34(1):60-9.

Mouse anti Dog IgE:Biotin

Product Type
Monoclonal Antibody
Clone
E6-71A1
Isotype
IgG1
Specificity
IgE

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Product Code Applications Pack Size List Price Your Price Qty
MCA1896B
Datasheet Datasheet Datasheet
SDS Safety Datasheet SDS
E WB 0.25 mg loader
List Price Your Price
loader

Mouse anti Dog IgE antibody, clone E6-71A1 recognises canine IgE. E6-2A1 does not cross react with dog IgM, IgA, IgG1 or IgG2. Western blot analysis against affinity purified dog IgE using Mouse anti Dog IgE clone E6-71A1 demonstrates a single major band of ~62-65 kDa under reducing conditions.

Target Species
Dog
Product Form
Purified IgG conjugated to biotin - liquid
Preparation
Purified IgG prepared by affinity chromatography on Protein A from tissue culture supernatant
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
<0.1% sodium azide (NaN3)
Immunogen
Affinity purified IgE preparation from heavily parasitized and allergic dog serum.
Approx. Protein Concentrations
IgG concentration 1.0 mg/ml
Regulatory
For research purposes only
Guarantee
12 months from date of despatch

This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.

Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
ELISA
Western Blotting
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
ELISA
Mouse anti Dog IgE antibody, clone E6-71A1 may be used as a detection antibody in a sandwich ELISA in combination with MCA1895 as the capture antibody.
Western Blotting
Mouse anti Dog IgE antibody, clone E6-71A1 detects a band of approximately 62-65 kDa in samples of affinity purified dog IgE under reducing conditions..

References for IgE antibody

  1. Ognjenovic, J. et al. (2013) Immunoproteomic characterization of Ambrosia artemisiifolia pollen allergens in canine atopic dermatitis.
    Vet Immunol Immunopathol. 155: 38-47.
  2. Martins, L.M. et al. (2017) Allergy to grass pollen: mapping of Dactylis glomerata and Phleum pratense allergens for dogs by two-dimensional immunoblotting.
    Postepy Dermatol Alergol. 34 (1): 60-9.

RRID
AB_323085

MCA1896B

147472 159139 162135 165272

If you cannot find the batch/lot you are looking for please contact our technical support team for assistance.

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Please Note: All Products are "FOR RESEARCH PURPOSES ONLY"

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