IgG1 antibody | IL-A60
Mouse anti Bovine IgG1
- Product Type
- Monoclonal Antibody
- Clone
- IL-A60
- Isotype
- IgG1
- Specificity
- IgG1
Mouse anti Bovine IgG1 antibody, clone IL-A60 recognizes bovine IgG1. Mouse anti Bovine IgG1 antibody, clone IL-A60 immunoprecipitates a protein band of 55-59 kDa, consistent with the heavy chain of bovine IgG1 (Campbell et al. 1998). |
- Target Species
- Bovine
- Product Form
- Purified IgG - liquid
- Preparation
- Purified IgG prepared by affinity chromatography on Protein A from tissue culture supernatant
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
- 0.09% Sodium Azide (NaN3)
- Carrier Free
- Yes
- Immunogen
- Purified bovine immunoglobulin.
- Approx. Protein Concentrations
- IgG concentration 1.0 mg/ml
- Fusion Partners
- Spleen cells from immunized BALB/c mice were fused with cells of the X63.Ag.653 myeloma cell line.
- Regulatory
- For research purposes only
- Guarantee
- 12 months from date of despatch
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.
Application Name | Verified | Min Dilution | Max Dilution |
---|---|---|---|
ELISA | 1/500 | 1/150K | |
Flow Cytometry | |||
Immunohistology - Frozen | |||
Immunoprecipitation |
- Flow Cytometry
- Use 10ul of the suggested working dilution to label 1x106 cells in 100ul
Description | Product Code | Applications | Pack Size | List Price | Your Price | Quantity | |
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Mouse IgG1 Negative Control | MCA928 | F | 100 Tests | Log in | |||
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Description | Mouse IgG1 Negative Control |
Description | Product Code | Applications | Pack Size | List Price | Your Price | Quantity | |
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Mouse anti Bovine IgA | MCA2438GA | E IP | 0.1 mg | Log in | |||
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Description | Mouse anti Bovine IgA | ||||||
Mouse anti Bovine IgA:HRP | MCA2438P | E | 0.25 mg | Log in | |||
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Description | Mouse anti Bovine IgA:HRP | ||||||
Mouse anti Bovine IgG | MCA2439GA | E IP | 0.1 mg | Log in | |||
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Description | Mouse anti Bovine IgG | ||||||
Mouse anti Bovine IgG:HRP | MCA2439P | E | 0.25 mg | Log in | |||
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Description | Mouse anti Bovine IgG:HRP | ||||||
Mouse anti Bovine IgG1:HRP | MCA2440P | E | 0.25 mg | Log in | |||
List Price | Your Price | ||||||
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Description | Mouse anti Bovine IgG1:HRP | ||||||
Mouse anti Bovine IgG2 | MCA2441GA | E F IP | 0.1 mg | Log in | |||
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Description | Mouse anti Bovine IgG2 | ||||||
Mouse anti Bovine IgG2:HRP | MCA2441P | E | 0.25 mg | Log in | |||
List Price | Your Price | ||||||
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Description | Mouse anti Bovine IgG2:HRP | ||||||
Mouse anti Bovine IgM | MCA2443GA | E F IP R | 0.1 mg | Log in | |||
List Price | Your Price | ||||||
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Description | Mouse anti Bovine IgM | ||||||
Mouse anti Sheep IgE | MCA5941GA | C E IF WB | 0.1 mg | Log in | |||
List Price | Your Price | ||||||
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Description | Mouse anti Sheep IgE |
Source Reference
-
Williams, D.J. et al. (1990) Quantitation of bovine immunoglobulin isotypes and allotypes using monoclonal antibodies.
Vet Immunol Immunopathol. 24 (3): 267-83.
References for IgG1 antibody
-
Williams, D.J. et al. (1996) The role of anti-variable surface glycoprotein antibody responses in bovine trypanotolerance.
Parasite Immunol. 18 (4): 209-18. -
Campbell, J.D. et al. (1998) A novel cell surface proliferation-associated marker expressed on T cells and up-regulated on germinal center B cells.
J Leukoc Biol. 63 (5): 567-74. -
Hecker YP et al. (2014) A Neospora caninum vaccine using recombinant proteins fails to prevent foetal infection in pregnant cattle after experimental intravenous challenge.
Vet Immunol Immunopathol. 162 (3-4): 142-53. -
Dorneles, E.M. et al. (2015) Immune Response of Calves Vaccinated with Brucella abortus S19 or RB51 and Revaccinated with RB51.
PLoS One. 10 (9): e0136696. -
Jaramillo, J.O. et al. (2019) Immunisation of cattle against Babesia bovis. combining a multi-epitope modified vaccinia Ankara virus and a recombinant protein induce strong Th1 cell responses but fails to trigger neutralising antibodies required for protection.
Ticks Tick Borne Dis. 10 (6): 101270. -
Pereyra, R. et al. (2019) Evidence of reduced vertical transmission of Neospora caninum. associated with higher IgG1 than IgG2 serum levels and presence of IFN-γ in non-aborting chronically infected cattle under natural condition.
Vet Immunol Immunopathol. 208: 53-57. -
Hecker, Y.P. et al. (2019) Immune response to Neospora caninum live tachyzoites in prepubertal female calves.
Parasitol Res. 118 (10): 2945-55. -
Villa-Mancera, A. et al. (2021) Phage display-based vaccine with cathepsin L and excretory-secretory products mimotopes of Fasciola hepatica. induces protective cellular and humoral immune responses in sheep.
Vet Parasitol. 289: 109340.
View The Latest Product References
-
Di Giacomo, S. et al. (2022) Assessment on Different Vaccine Formulation Parameters in the Protection against Heterologous Challenge with FMDV in Cattle.
Viruses. 14 (8): 1781. -
Pooley, H.B. et al. (2019) The humoral immune response is essential for successful vaccine protection against paratuberculosis in sheep.
BMC Vet Res. 15 (1): 223.
MCA2440GA
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