Mouse anti Bovine IgA antibody, clone K84 2F9 recognizes bovine and ovine IgA. No cross reactivity is observed with Bovine or Ovine IgG and IgM,
- Target Species
- Species Cross-Reactivity
|Target Species||Cross Reactivity|
- N.B. Antibody reactivity and working conditions may vary between species.
- Product Form
- Purified IgG - liquid
- Purified IgG prepared by affinity chromatography on Protein A from tissue culture supernatant
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
- 0.09% Sodium Azide (NaN3)
- Carrier Free
- Bovine IgA.
- Approx. Protein Concentrations
- IgG concentration 1.0 mg/ml
- Fusion Partners
- Spleen cells from immunised mice were fused with cells of the P3.X63.Ag8.653 myeloma cell line.
- This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.
- 12 months from date of despatch
- For research purposes only
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Applications of IgA antibody
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
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Secondary Antibodies Available
Useful Reagents Available
Product Specific References
References for IgA antibody
Estes, D.M. et al. (1998) Effects of type I/type II interferons and transforming growth factor-beta on B-cell differentiation and proliferation. Definition of costimulation and cytokine requirements for immunoglobulin synthesis and expression.
Immunology. 95 (4): 604-11.
Suraud, V. et al. (2008) Acute infection by conjunctival route with Brucella melitensis induces IgG+ cells and IFN-gamma producing cells in peripheral and mucosal lymph nodes in sheep.
Microbes Infect. 10: 1370-8.
Hassan, M. et al. (2011) The dynamic influence of the DRB1*1101 allele on the resistance of sheep to experimental Teladorsagia circumcincta infection.
Vet Res. 42: 46.
Tzelos, T. et al. (2016) A preliminary proteomic characterisation of extracellular vesicles released by the ovine parasitic nematode, Teladorsagia circumcincta
<a rel="nofollow" href="http://www.sciencedirect.com/science/article/pii/S0304401716300619"target="_blank">Veterinary Parasitology. Mar 17 [Epub ahead of print]</a>
Stanley AC et al. (2004) Intranasal immunisation with Toxoplasma gondii tachyzoite antigen encapsulated into PLG microspheres induces humoral and cell-mediated immunity in sheep.
Vaccine. 22 (29-30): 3929-41.
McNeilly, T.N. et al. (2007) Simple methods for measurement of bovine mucosal antibody responses in vivo.
Vet Immunol Immunopathol. 118 (1-2): 160-7.
McNeilly, T.N. et al. (2010) IgA and IgG antibody responses following systemic immunization of cattle with native H7 flagellin differ in epitope recognition and capacity to neutralise TLR5 signalling.
Vaccine. 28 (5): 1412-21.
McNeilly, T.N. et al. (2010) Immunization of cattle with a combination of purified intimin-531, EspA and Tir significantly reduces shedding of Escherichia coli O157:H7 following oral challenge.
Vaccine. 28 (5): 1422-8.
Suraud, V. et al. (2007) Differential expression of homing receptors and vascular addressins in tonsils and draining lymph nodes: Effect of Brucella infection in sheep.
Vet Immunol Immunopathol. 115 (3-4): 239-50.
Mahajan, A. et al. (2005) Phenotypic and functional characterisation of follicle-associated epithelium of rectal lymphoid tissue.
Cell Tissue Res. 321 (3): 365-74.
Parreño, V.et al. (2004) Modulation by colostrum-acquired maternal antibodies of systemic and mucosal antibody responses to rotavirus in calves experimentally challenged with bovine rotavirus.
Vet Immunol Immunopathol. 100 (1-2): 7-24.
Mahajan, A. et al. (2009) An investigation of the expression and adhesin function of H7 flagella in the interaction of Escherichia coli O157 : H7 with bovine intestinal epithelium.
Cell Microbiol. 11 (1): 121-37.
McNeilly, T.N. et al. (2013) Suppression of ovine lymphocyte activation by Teladorsagia circumcincta larval excretory-secretory products.
Vet Res. 44: 70.
Nisbet, A. J. et al. (2016) Protection of ewes against Teladorsagia circumcincta infection in the periparturient period by vaccination with recombinant antigens
Veterinary Parasitology. 228: 130-6.
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