IgA antibody | K84 2F9
Mouse anti Bovine IgA antibody, clone K84 2F9 recognizes bovine and ovine IgA. No cross reactivity is observed with Bovine or Ovine IgG and IgM,
- Target Species
- Species Cross-Reactivity
Target Species Cross Reactivity Sheep
- N.B. Antibody reactivity and working conditions may vary between species.
- Product Form
- Purified IgG - liquid
- Purified IgG prepared by affinity chromatography on Protein A from tissue culture supernatant
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
- 0.09% Sodium Azide (NaN3)
- Carrier Free
- Bovine IgA.
- Approx. Protein Concentrations
- IgG concentration 1.0 mg/ml
- Fusion Partners
- Spleen cells from immunised mice were fused with cells of the P3.X63.Ag8.653 myeloma cell line.
- Store at +4oC or at -20oC if preferred.
Storage in frost-free freezers is not recommended.
This product should be stored undiluted. Should this product contain a precipitate we recommend microcentrifugation before use.
- Shelf Life
- 18 months from date of despatch
- For research purposes only
Applications of IgA antibody
|Application Name||Verified||Min Dilution||Max Dilution|
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
Copyright © 2018 Bio-Rad Antibodies (formerly AbD Serotec)
Secondary Antibodies Available
Useful Reagents Available
|Description||Product Code||Pack Size||Applications||List Price||Quantity|
|Mouse anti Bovine IgA||MCA2438GA||0.1 mg||E IP|
|Mouse anti Bovine IgA:HRP||MCA2438P||0.25 mg||E|
|Mouse anti Bovine IgG||MCA2439GA||0.1 mg||E IP|
|Mouse anti Bovine IgG:HRP||MCA2439P||0.25 mg||E|
|Mouse anti Bovine IgG1||MCA2440GA||0.1 mg||C E F IP|
|Mouse anti Bovine IgG1:HRP||MCA2440P||0.25 mg||E|
|Mouse anti Bovine IgG2||MCA2441GA||0.1 mg||E F IP|
|Mouse anti Bovine IgG2:HRP||MCA2441P||0.25 mg||E|
|Mouse anti Bovine IgM||MCA2443GA||0.1 mg||E F IP R|
|Mouse anti Sheep IgE||MCA5941GA||0.1 mg||C E IF WB|
|Mouse anti Bovine IgG1||MCA627GA||0.1 mg||E|
Product Specific References
References for IgA antibody
Estes, D.M. et al. (1998) Effects of type I/type II interferons and transforming growth factor-beta on B-cell differentiation and proliferation. Definition of costimulation and cytokine requirements for immunoglobulin synthesis and expression.
Immunology. 95 (4): 604-11.
Suraud, V. et al. (2008) Acute infection by conjunctival route with Brucella melitensis induces IgG+ cells and IFN-gamma producing cells in peripheral and mucosal lymph nodes in sheep.
Microbes Infect. 10: 1370-8.
Hassan, M. et al. (2011) The dynamic influence of the DRB1*1101 allele on the resistance of sheep to experimental Teladorsagia circumcincta infection.
Vet Res. 42: 46.
Tzelos, T. et al. (2016) A preliminary proteomic characterisation of extracellular vesicles released by the ovine parasitic nematode, Teladorsagia circumcincta
<a rel="nofollow" href="http://www.sciencedirect.com/science/article/pii/S0304401716300619"target="_blank">Veterinary Parasitology. Mar 17 [Epub ahead of print]</a>
Stanley AC et al. (2004) Intranasal immunisation with Toxoplasma gondii tachyzoite antigen encapsulated into PLG microspheres induces humoral and cell-mediated immunity in sheep.
Vaccine. 22 (29-30): 3929-41.
McNeilly, T.N. et al. (2007) Simple methods for measurement of bovine mucosal antibody responses in vivo.
Vet Immunol Immunopathol. 118 (1-2): 160-7.
McNeilly, T.N. et al. (2010) IgA and IgG antibody responses following systemic immunization of cattle with native H7 flagellin differ in epitope recognition and capacity to neutralise TLR5 signalling.
Vaccine. 28 (5): 1412-21.
McNeilly, T.N. et al. (2010) Immunization of cattle with a combination of purified intimin-531, EspA and Tir significantly reduces shedding of Escherichia coli O157:H7 following oral challenge.
Vaccine. 28 (5): 1422-8.
Suraud, V. et al. (2007) Differential expression of homing receptors and vascular addressins in tonsils and draining lymph nodes: Effect of Brucella infection in sheep.
Vet Immunol Immunopathol. 115 (3-4): 239-50.
Mahajan, A. et al. (2005) Phenotypic and functional characterisation of follicle-associated epithelium of rectal lymphoid tissue.
Cell Tissue Res. 321 (3): 365-74.
Parreño, V.et al. (2004) Modulation by colostrum-acquired maternal antibodies of systemic and mucosal antibody responses to rotavirus in calves experimentally challenged with bovine rotavirus.
Vet Immunol Immunopathol. 100 (1-2): 7-24.
Mahajan, A. et al. (2009) An investigation of the expression and adhesin function of H7 flagella in the interaction of Escherichia coli O157 : H7 with bovine intestinal epithelium.
Cell Microbiol. 11 (1): 121-37.
McNeilly, T.N. et al. (2013) Suppression of ovine lymphocyte activation by Teladorsagia circumcincta larval excretory-secretory products.
Vet Res. 44: 70.
Nisbet, A. J. et al. (2016) Protection of ewes against Teladorsagia circumcincta infection in the periparturient period by vaccination with recombinant antigens
Veterinary Parasitology. 228: 130-6.