IgA antibody | K84 2F9

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Mouse anti Bovine/Ovine IgA

Product Type
Monoclonal Antibody
Clone
K84 2F9
Isotype
IgG1
Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
MCA628GA E 0.1 mg
Mouse anti Bovine IgA antibody, clone K84 2F9 recognizes bovine and ovine IgA. No cross reactivity is observed with Bovine or Ovine IgG and IgM,

Product Details

Target Species
Bovine
Species Cross-Reactivity
Target SpeciesCross Reactivity
Sheep
N.B. Antibody reactivity and working conditions may vary between species.
Product Form
Purified IgG - liquid
Preparation
Purified IgG prepared by affinity chromatography on Protein A from tissue culture supernatant
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.09% Sodium Azide (NaN3)
Carrier Free
Yes
Immunogen
Bovine IgA.
Approx. Protein Concentrations
IgG concentration 1.0 mg/ml
Fusion Partners
Spleen cells from immunised mice were fused with cells of the P3.X63.Ag8.653 myeloma cell line.

Storage Information

Storage
Store at +4oC or at -20oC if preferred.
Storage in frost-free freezers is not recommended.
This product should be stored undiluted. Should this product contain a precipitate we recommend microcentrifugation before use.
Shelf Life
18 months from date of despatch

More Information

Regulatory
For research purposes only

Applications of IgA antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
ELISA 1/5000 1/80,000
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.

Secondary Antibodies Available

Description Product Code Pack Size Applications List Price Quantity
Human anti Mouse IgG1:HRP HCA036P 0.1 mg E
Goat anti Mouse IgG (H/L):Alk. Phos. (Multi Species Adsorbed) STAR117A 0.5 mg E WB
Goat anti Mouse IgG (H/L):DyLight®488 (Multi Species Adsorbed) STAR117D488GA 0.1 mg F IF
Goat anti Mouse IgG (H/L):DyLight®549 (Multi Species Adsorbed) STAR117D549GA 0.1 mg F IF WB
Goat anti Mouse IgG (H/L):DyLight®649 (Multi Species Adsorbed) STAR117D649GA 0.1 mg F IF
Goat anti Mouse IgG (H/L):DyLight®680 (Multi Species Adsorbed) STAR117D680GA 0.1 mg F WB
Goat anti Mouse IgG (H/L):DyLight®800 (Multi Species Adsorbed) STAR117D800GA 0.1 mg F IF WB
Goat anti Mouse IgG (H/L):FITC (Multi Species Adsorbed) STAR117F 0.5 mg F
Goat anti Mouse IgG (H/L):HRP (Multi Species Adsorbed) STAR117P 0.5 mg E WB
Goat anti Mouse IgG (Fc):FITC STAR120F 1 mg C F
Goat anti Mouse IgG (Fc):HRP STAR120P 1 mg E WB
Rabbit F(ab')2 anti Mouse IgG:RPE STAR12A 1 ml F
Rabbit F(ab')2 anti Mouse IgG:HRP (Human Adsorbed) STAR13B 1 mg C E P RE WB
Goat anti Mouse IgG:FITC (Rat Adsorbed) STAR70 0.5 mg F
Goat anti Mouse IgG:RPE (Rat Adsorbed) STAR76 1 ml F
Goat anti Mouse IgG:HRP (Rat Adsorbed) STAR77 0.5 mg C E P
Goat anti Mouse IgG/A/M:Alk. Phos. STAR87A 1 mg C E WB
Goat anti Mouse IgG/A/M:HRP (Human Adsorbed) STAR87P 1 mg E
Rabbit F(ab')2 anti Mouse IgG:Dylight®800 STAR8D800GA 0.1 mg F IF WB
Rabbit F(ab')2 anti Mouse IgG:FITC STAR9B 1 mg F

Useful Reagents Available

Description Product Code Pack Size Applications List Price Quantity
Mouse anti Bovine IgA MCA2438GA 0.1 mg E IP
Mouse anti Bovine IgA:HRP MCA2438P 0.25 mg E
Mouse anti Bovine IgG MCA2439GA 0.1 mg E IP
Mouse anti Bovine IgG:HRP MCA2439P 0.25 mg E
Mouse anti Bovine IgG1 MCA2440GA 0.1 mg C E F IP
Mouse anti Bovine IgG1:HRP MCA2440P 0.25 mg E
Mouse anti Bovine IgG2 MCA2441GA 0.1 mg E F IP
Mouse anti Bovine IgG2:HRP MCA2441P 0.25 mg E
Mouse anti Bovine IgM MCA2443GA 0.1 mg E F IP R
Mouse anti Sheep IgE MCA5941GA 0.1 mg C E IF WB
Mouse anti Bovine IgG1 MCA627GA 0.1 mg E

Product Specific References

References for IgA antibody

  1. Estes, D.M. et al. (1998) Effects of type I/type II interferons and transforming growth factor-beta on B-cell differentiation and proliferation. Definition of costimulation and cytokine requirements for immunoglobulin synthesis and expression.
    Immunology. 95 (4): 604-11.
  2. Suraud, V. et al. (2008) Acute infection by conjunctival route with Brucella melitensis induces IgG+ cells and IFN-gamma producing cells in peripheral and mucosal lymph nodes in sheep.
    Microbes Infect. 10: 1370-8.
  3. Hassan, M. et al. (2011) The dynamic influence of the DRB1*1101 allele on the resistance of sheep to experimental Teladorsagia circumcincta infection.
    Vet Res. 42: 46.
  4. Tzelos, T. et al. (2016) A preliminary proteomic characterisation of extracellular vesicles released by the ovine parasitic nematode, Teladorsagia circumcincta
    <a rel="nofollow" href="http://www.sciencedirect.com/science/article/pii/S0304401716300619"target="_blank">Veterinary Parasitology. Mar 17 [Epub ahead of print]</a>
  5. Stanley AC et al. (2004) Intranasal immunisation with Toxoplasma gondii tachyzoite antigen encapsulated into PLG microspheres induces humoral and cell-mediated immunity in sheep.
    Vaccine. 22 (29-30): 3929-41.
  6. McNeilly, T.N. et al. (2007) Simple methods for measurement of bovine mucosal antibody responses in vivo.
    Vet Immunol Immunopathol. 118 (1-2): 160-7.
  7. McNeilly, T.N. et al. (2010) IgA and IgG antibody responses following systemic immunization of cattle with native H7 flagellin differ in epitope recognition and capacity to neutralise TLR5 signalling.
    Vaccine. 28 (5): 1412-21.
  8. McNeilly, T.N. et al. (2010) Immunization of cattle with a combination of purified intimin-531, EspA and Tir significantly reduces shedding of Escherichia coli O157:H7 following oral challenge.
    Vaccine. 28 (5): 1422-8.
  9. Suraud, V. et al. (2007) Differential expression of homing receptors and vascular addressins in tonsils and draining lymph nodes: Effect of Brucella infection in sheep.
    Vet Immunol Immunopathol. 115 (3-4): 239-50.
  10. Mahajan, A. et al. (2005) Phenotypic and functional characterisation of follicle-associated epithelium of rectal lymphoid tissue.
    Cell Tissue Res. 321 (3): 365-74.
  11. Parreño, V.et al. (2004) Modulation by colostrum-acquired maternal antibodies of systemic and mucosal antibody responses to rotavirus in calves experimentally challenged with bovine rotavirus.
    Vet Immunol Immunopathol. 100 (1-2): 7-24.
  12. Mahajan, A. et al. (2009) An investigation of the expression and adhesin function of H7 flagella in the interaction of Escherichia coli O157 : H7 with bovine intestinal epithelium.
    Cell Microbiol. 11 (1): 121-37.
  13. McNeilly, T.N. et al. (2013) Suppression of ovine lymphocyte activation by Teladorsagia circumcincta larval excretory-secretory products.
    Vet Res. 44: 70.
  14. Nisbet, A. J. et al. (2016) Protection of ewes against Teladorsagia circumcincta infection in the periparturient period by vaccination with recombinant antigens
    Veterinary Parasitology. 228: 130-6.