CD40 antibody | IL-A156

Product Code Applications Pack Size List Price Your Price Qty
MCA2431GA
Datasheet Datasheet Datasheet
SDS Safety Datasheet SDS
F FN * 0.1 mg loader
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loader
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Mouse anti Bovine CD40 antibody, clone IL-A156 recognizes the bovine Tumor necrosis factor receptor superfamily member 5, also known as CD40 or B-cell surface antigen CD40. CD40 is a 260 amino acid single pass type 1 transmembrane glycoprotein with a predicted molecular mass of 31 kDa. Bovine CD40 also has an N terminal signal peptide of 20 amino acids. Bovine CD40 is expressed by B lymphocytes and a subset of T lymphocytes.

CD40 plays an important role in the differentiation of B lymphocytes into effector cells, and is also involved in interactions between T and B lymphocytes.

Target Species
Bovine
Species Cross-Reactivity
Target SpeciesCross Reactivity
Sheep
N.B. Antibody reactivity and working conditions may vary between species.
Product Form
Purified IgG - liquid
Preparation
Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.09% Sodium Azide (NaN3)
Carrier Free
Yes
Approx. Protein Concentrations
IgG concentration 1.0 mg/ml
Fusion Partners
Spleen cells from immunised BALB/c mice were fused with cells of the X63.Ag8.653 myeloma cell line
Regulatory
For research purposes only
Guarantee
12 months from date of despatch

This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.

Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Flow Cytometry 1/25 1/200
Functional Assays 1
  1. 1 Clone IL-A156 is reported to function in in vivo applications, see Haas, K. et al. for details. Removal of sodium azide is recommended prior to use in functional assays.
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
Flow Cytometry
Use 10ul of the suggested working dilution to label 1x106 cells in 100ul

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References for CD40 antibody

  1. Langelaar, M.F. et al. (2005) Mycobacterium avium ssp. paratuberculosis recombinant heat shock protein 70 interaction with different bovine antigen-presenting cells.
    Scand J Immunol. 61 (3): 242-50.
  2. Epardaud, M. et al. (2004) Enrichment for a CD26hi SIRP- subset in lymph dendritic cells from the upper aero-digestive tract.
    J Leukoc Biol. 76 (3): 553-61.
  3. Glew, E.J. et al. (2003) Differential effects of bovine viral diarrhoea virus on monocytes and dendritic cells.
    J Gen Virol. 84 (Pt 7): 1771-80.
  4. Norimatsu, M. et al. (2003) Differential response of bovine monocyte-derived macrophages and dendritic cells to infection with Salmonella typhimurium in a low-dose model in vitro.
    Immunology 108: 55- 61.
  5. Haas, K.M. et al. (2001) Enhancing effects of anti-CD40 treatment on the immune response of SCID-bovine mice to Trypanosoma congolense infection.
    J Leukoc Biol. 70: 931-40.
  6. Totté, P. et al. (2015) Free exopolysaccharide from Mycoplasma mycoides subsp. mycoides possesses anti-inflammatory properties.
    Vet Res. 46 (1): 122.
  7. Corripio-Miyar, Y. et al. (2015) Phenotypic and functional analysis of monocyte populations in cattle peripheral blood identifies a subset with high endocytic and allogeneic T-cell stimulatory capacity.
    Vet Res. 46: 112.
  8. Marzo, S. et al. (2021) Characterisation of dendritic cell frequency and phenotype in bovine afferent lymph reveals kinetic changes in costimulatory molecule expression
    Vet Immunol Immunopathol. 19 Nov: 110363.
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  10. Kornuta, C.A. et al. (2021) MANα1-2MAN decorated liposomes enhance the immunogenicity induced by a DNA vaccine against BoHV-1.
    Transbound Emerg Dis. 68 (2): 587-97.
  11. Liu, J. et al. (2020) Theileria annulata. transformation altered cell surface molecules expression and endocytic function of monocyte-derived dendritic cells.
    Ticks Tick Borne Dis. 11 (3): 101365.

Flow Cytometry

RRID
AB_11152595
UniProt
Q28203
Entrez Gene
CD40
GO Terms
GO:0016021 integral to membrane
GO:0004872 receptor activity
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