CD40 antibody | IL-A156
Mouse anti Bovine CD40 antibody, clone IL-A156 (MCA2431GA) used for the identification of CD40 expressing lymph node cells from naïve and contagious bovine pleuropneumonia infected cattle
Image caption:
Effect of free Mmm galactan on surface expression of CD40 ( A ) and CD80 ( B ) on bovine macrophages. Cells were incubated for 48 h with galactan (EPS) at 0.1, 1 and 10 μg/ml, or with LPS and medium only (C−) as positive and negative controls respectively. Cell-surface expression of CD40/CD80 was measured by flow cytometry and results expressed as mean (±SD) percentages of positive cells for a given marker (n = 3). Asterisks indicate statistically significant differences (p < 0.05) with the negative control.
From: Totté P, Puech C, Rodrigues V, Bertin C, Manso-Silvan L, Thiaucourt F.
Free exopolysaccharide from Mycoplasma mycoides subsp. mycoides possesses anti-inflammatory properties.
Vet Res. 2015 Oct 21;46(1):122.
This is from an open access article distributed under the terms of the Creative Commons Attribution License.
Mouse anti Bovine CD40 antibody, clone IL-A156 (MCA2431GA) used to assess CD40 expression on bovine PBMCs by flow cytometry.
Image caption:
Phenotypic profiles of bovine myeloid cell sub-populations. Live gated PBMC were assessed for expression of CD16 and CD14 and a panel of molecules associated with antigen presentation, co-stimulation or specific cell lineages (see Table 1) by 3 colour flow cytometry. PBMC were stained with primary mAb to the specific molecules indicated and then with an isotype-specific PE conjugated secondary, followed by CD16 and CD14 conjugated to FITC or Alexa Fluor 647 respectively. Live, single PBMC were gated based on the expression of CD16 and CD14 as detailed in Figures 1 and and2C.2C. Histograms show the levels of expression of selected markers in the cell populations studied, CD14−CD16++ (red), CD14+CD16+ (blue) and CD14+CD16low/- (orange) compared to PBMC (black). Data shown are for one representative animal of four animals.
From: Corripio-Miyar Y, Hope J, McInnes CJ, Wattegedera SR, Jensen K, Pang Y, Entrican G, Glass EJ.
Phenotypic and functional analysis of monocyte populations in cattle peripheral blood identifies a subset with high endocytic and allogeneic T-cell stimulatory capacity.
Vet Res. 2015 Sep 25;46:112.
doi: 10.1186/s13567-015-0246-4.
This image is from an open access article distributed under the terms of the Creative Commons Attribution License.
Mouse anti Bovine CD40
- Product Type
- Monoclonal Antibody
- Clone
- IL-A156
- Isotype
- IgG1
- Specificity
- CD40
Antibody Quality and Performance Guarantee
| Mouse anti Bovine CD40 antibody, clone IL-A156 recognizes the bovine Tumor necrosis factor receptor superfamily member 5, also known as CD40 or B-cell surface antigen CD40. CD40 is a 260 amino acid single pass type 1 transmembrane glycoprotein with a predicted molecular mass of 31 kDa. Bovine CD40 also has an N terminal signal peptide of 20 amino acids. Bovine CD40 is expressed by B lymphocytes and a subset of T lymphocytes. CD40 plays an important role in the differentiation of B lymphocytes into effector cells, and is also involved in interactions between T and B lymphocytes. |
- Target Species
- Bovine
- Species Cross-Reactivity
-
Target Species Cross Reactivity Sheep - N.B. Antibody reactivity and working conditions may vary between species.
- Product Form
- Purified IgG - liquid
- Preparation
- Purified IgG prepared by affinity chromatography on Protein A from tissue culture supernatant
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
- 0.09% Sodium Azide (NaN3)
- Carrier Free
- Yes
- Approx. Protein Concentrations
- IgG concentration 1.0 mg/ml
- Fusion Partners
- Spleen cells from immunised BALB/c mice were fused with cells of the X63.Ag8.653 myeloma cell line
- Regulatory
- For research purposes only
- Guarantee
- 12 months from date of despatch
This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
| Application Name | Verified | Min Dilution | Max Dilution |
|---|---|---|---|
| Flow Cytometry |  |
1/25 | 1/200 |
| Functional Assays 1 | |
- 1 Clone IL-A156 is reported to function in in vivo applications, see Haas, K. et al. for details. Removal of sodium azide is recommended prior to use in functional assays.
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
- Flow Cytometry
- Use 10ul of the suggested working dilution to label 1x106 cells in 100ul
| Description | Product Code | Applications | Pack Size | List Price | Your Price | Quantity | |
|---|---|---|---|---|---|---|---|
| Mouse IgG1 Negative Control | MCA928 | F | 100 Tests |
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| Description | Mouse IgG1 Negative Control | ||||||
| Description | Product Code | Applications | Pack Size | List Price | Your Price | Quantity | |
|---|---|---|---|---|---|---|---|
| Mouse anti Bovine CD80:FITC | MCA2436F | F | 0.1 mg |
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| Description | Mouse anti Bovine CD80:FITC | ||||||
| Mouse anti Bovine CD80 | MCA2436GA | F | 0.1 mg |
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| Description | Mouse anti Bovine CD80 | ||||||
| Mouse anti Bovine CD80:RPE | MCA2436PE | F | 100 Tests | Log in | |||
| List Price | Your Price | ||||||
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| Description | Mouse anti Bovine CD80:RPE | ||||||
| Mouse anti Bovine CD86:FITC | MCA2437F | F | 0.1 mg |
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| Description | Mouse anti Bovine CD86:FITC | ||||||
| Mouse anti Bovine CD86 | MCA2437GA | F | 0.1 mg |
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| Description | Mouse anti Bovine CD86 | ||||||
| Mouse anti Bovine CD86:RPE | MCA2437PE | F | 100 Tests | Log in | |||
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| Description | Mouse anti Bovine CD86:RPE | ||||||
| Mouse anti Bovine CD28 | MCA5778 | F FN | 0.25 mg |
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| Description | Mouse anti Bovine CD28 | ||||||
| Mouse anti Bovine CD28:FITC | MCA5779F | F | 0.1 mg |
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| Description | Mouse anti Bovine CD28:FITC | ||||||
| Mouse anti Bovine CD28:RPE | MCA5779PE | F | 100 Test | Log in | |||
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| Description | Mouse anti Bovine CD28:RPE | ||||||
References for CD40 antibody
-
Haas, K.M. et al. (2001) Enhancing effects of anti-CD40 treatment on the immune response of SCID-bovine mice to Trypanosoma congolense infection.
J Leukoc Biol. 70: 931-40. -
Norimatsu, M. et al. (2003) Differential response of bovine monocyte-derived macrophages and dendritic cells to infection with Salmonella typhimurium in a low-dose model in vitro.
Immunology 108: 55- 61. -
Glew, E.J. et al. (2003) Differential effects of bovine viral diarrhoea virus on monocytes and dendritic cells.
J Gen Virol. 84 (Pt 7): 1771-80. -
Epardaud, M. et al. (2004) Enrichment for a CD26hi SIRP- subset in lymph dendritic cells from the upper aero-digestive tract.
J Leukoc Biol. 76 (3): 553-61. -
Langelaar, M.F. et al. (2005) Mycobacterium avium ssp. paratuberculosis recombinant heat shock protein 70 interaction with different bovine antigen-presenting cells.
Scand J Immunol. 61 (3): 242-50. -
Corripio-Miyar, Y. et al. (2015) Phenotypic and functional analysis of monocyte populations in cattle peripheral blood identifies a subset with high endocytic and allogeneic T-cell stimulatory capacity.
Vet Res. 46: 112. -
Totté, P. et al. (2015) Free exopolysaccharide from Mycoplasma mycoides subsp. mycoides possesses anti-inflammatory properties.
Vet Res. 46 (1): 122. -
Liu, J. et al. (2020) Theileria annulata. transformation altered cell surface molecules expression and endocytic function of monocyte-derived dendritic cells.
Ticks Tick Borne Dis. 11 (3): 101365.
View The Latest Product References
-
Kornuta, C.A. et al. (2021) MANα1-2MAN decorated liposomes enhance the immunogenicity induced by a DNA vaccine against BoHV-1.
Transbound Emerg Dis. 68 (2): 587-97. -
Marzo, S. et al. (2021) Characterisation of dendritic cell frequency and phenotype in bovine afferent lymph reveals kinetic changes in costimulatory molecule expression
Vet Immunol Immunopathol. 19 Nov: 110363.
- RRID
- AB_11152595
- UniProt
- Q28203
- Entrez Gene
- CD40
- GO Terms
- GO:0016021 integral to membrane
- GO:0004872 receptor activity
MCA2431GA
161438
167892
1702
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