CD28 antibody | CC220

Mouse anti Bovine CD28

Product Type
Monoclonal Antibody
Clone
CC220
Isotype
IgG1
Specificity
CD28

Product Code Applications Pack Size List Price Your Price Qty
MCA5778
Datasheet Datasheet Datasheet
SDS Safety Datasheet SDS
F FN 0.25 mg loader
List Price Your Price
loader
Search for Batch Specific Datasheets

Mouse anti Bovine CD28, clone CC220 recognizes the bovine homologue of human CD28, a co-stimulatory cell surface molecule expressed by T cell subsets.

CD28 is the receptor for CD80 (B7.1) and CD86 (B7.2). Mouse anti Bovine CD28, clone CC220 is able to stimulate bovine T lymphocytes in combination with anti-bovine CD3 (Nishimori et al. 2017).

For functional use, the use of the preservative free format (MCA5778EL) is recommended. Mouse anti Bovine CD28, clone CC220 has also been published under the alternative designation F849CD10 (Hogg et al. 2011 & Guzman et al. 2014).

Target Species
Bovine
Product Form
Purified IgG - liquid
Preparation
Purified IgG prepared by affinity chromatography on Protein A from tissue culture supernatant
Buffer Solution
Phosphate buffered saline.
Preservative Stabilisers
0.09% Sodium Azide (NaN3)
Carrier Free
Yes
Immunogen
Plasmid DNA encoding the bovine CD28 gene.
Approx. Protein Concentrations
IgG concentration 1.0 mg/ml
Fusion Partners
Spleen cells from immunised Balb/c mice were fused with cells of the SP2/0 myeloma cell line.
Regulatory
For research purposes only
Guarantee
12 months from date of despatch

This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.

Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Flow Cytometry
Functional Assays
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
Flow Cytometry
Use 10ul of the suggested working dilution to label 1x106 cells in 100ul.

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Description Goat anti Mouse IgG (H/L):DyLight®800 (Multi Species Adsorbed)
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Description Goat anti Mouse IgG (H/L):FITC (Multi Species Adsorbed)
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Description Goat anti Mouse IgG (H/L):HRP (Multi Species Adsorbed)
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Description Goat anti Mouse IgG (Fc):FITC
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Description Rabbit F(ab')2 anti Mouse IgG:HRP (Human Adsorbed)
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Description Goat anti Mouse IgG/A/M:Alk. Phos.
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Description Goat anti Mouse IgG/A/M:HRP (Human Adsorbed)
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Description Rabbit F(ab')2 anti Mouse IgG:Dylight®800
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Description Mouse IgG1 Negative Control

References for CD28 antibody

  1. Hogg, A.E. et al. (2011) Characterization of age-related changes in bovine CD8+ T-cells.
    Vet Immunol Immunopathol. 140 (1-2): 47-54.
  2. Goto, S. et al. (2017) Increase of cells expressing PD-1 and PD-L1 and enhancement of IFN-γ production via PD-1/PD-L1 blockade in bovine mycoplasmosis.
    Immun Inflamm Dis. 5 (3): 355-363.
  3. Nishimori, A. et al. (2017) In vitro and in vivo antivirus activity of an anti-programmed death-ligand 1 (PD-L1) rat-bovine chimeric antibody against bovine leukemia virus infection.
    PLoS One. 12 (4): e0174916.
  4. Cunha, P. et al. (2019) Expansion, isolation and first characterization of bovine Th17 lymphocytes.
    Sci Rep. 9 (1): 16115.

RRID
AB_10965063
UniProt
Q28071
Entrez Gene
CD28
GO Terms
GO:0016021 integral to membrane
GO:0006955 immune response
GO:0042102 positive regulation of T cell proliferation
GO:0045086 positive regulation of interleukin-2 biosynthetic process
GO:0045840 positive regulation of mitosis
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Please Note: All Products are "FOR RESEARCH PURPOSES ONLY"

View all Anti-Bovine Products