CD86 antibody | IL-A190

Mouse anti Bovine CD86:FITC

Product Type
Monoclonal Antibody
Clone
IL-A190
Isotype
IgG1
Specificity
CD86

Product Code Applications Pack Size List Price Your Price Qty
MCA2437F
Datasheet Datasheet Datasheet
SDS Safety Datasheet SDS
F 0.1 mg loader
List Price Your Price
loader

Mouse anti Bovine CD86 antibody, cllone IL-A190 recognises the bovine CD86 cell surface antigen, which is expressed by dendritic cells, activated macrophages and activated B cells. CD86 plays an important role in co-stimulation of T cells in the primary immune response.

Target Species
Bovine
Species Cross-Reactivity
Target SpeciesCross Reactivity
Sheep
N.B. Antibody reactivity and working conditions may vary between species.
Product Form
Purified IgG conjugated to Fluorescein Isothiocyanate Isomer 1 (FITC) - liquid
Preparation
Purified IgG prepared by affinity chromatography on Protein A from tissue culture supernatant
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.09% Sodium Azide (NaN3)
1% Bovine Serum Albumin
Approx. Protein Concentrations
IgG concentration 0.1 mg/ml
Fusion Partners
Spleen cells from immunised BALB/c mice were fused with cells of the X63.Ag8.653 myeloma cell line.
Max Ex/Em
Fluorophore Excitation Max (nm) Emission Max (nm)
FITC 490 525
Regulatory
For research purposes only
Guarantee
12 months from date of despatch

This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.

Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended. This product is photosensitive and should be protected from light.

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Flow Cytometry Neat 1/10
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
Flow Cytometry
Use 10ul of the suggested working dilution to label 1x106 cells in 100ul

Description Product Code Applications Pack Size List Price Your Price Quantity
Mouse IgG1 Negative Control:FITC MCA928F F 100 Tests loader
List Price Your Price
loader
Description Mouse IgG1 Negative Control:FITC

References for CD86 antibody

  1. Langelaar, M.F. et al. (2005) Mycobacterium avium ssp. paratuberculosis recombinant heat shock protein 70 interaction with different bovine antigen-presenting cells.
    Scand J Immunol. 61: 242-50
  2. Rhodes, S.G. et al. (2003) 1,25-dihydroxyvitamin D3 and development of tuberculosis in cattle.
    Clin Diagn Lab Immunol. 10 (6): 1129-35.
  3. Glew, E.J. et al. (2003) Differential effects of bovine viral diarrhoea virus on monocytes and dendritic cells.
    J Gen Virol. 84 (Pt 7): 1771-80.
  4. Epardaud, M. et al. (2004) Enrichment for a CD26hi SIRP- subset in lymph dendritic cells from the upper aero-digestive tract.
    J Leukoc Biol. 76 (3): 553-61.
  5. Bonneau, M. et al. (2006) Migratory monocytes and granulocytes are major lymphatic carriers of Salmonella from tissue to draining lymph node.
    J Leukoc Biol. 79: 268-76.
  6. Norimatsu, M. et al. (2003) Differential response of bovine monocyte-derived macrophages and dendritic cells to infection with Salmonella typhimurium in a low-dose model in vitro.
    Immunology. 108: 55-61.
  7. Hemati, B. et al. (2009) Bluetongue virus targets conventional dendritic cells in skin lymph.
    J Virol. 83: 8789-99.
  8. Pascale, F. et al. (2008) Plasmacytoid dendritic cells migrate in afferent skin lymph.
    J Immunol. 180: 5963-72.
  9. View The Latest Product References
  10. Ruscanu, S. et al. (2012) The double-stranded RNA bluetongue virus induces type I interferon in plasmacytoid dendritic cells via a MYD88-dependent TLR7/8-independent signaling pathway.
    J Virol. 2012 May;86: 5817-28.
  11. Mauro, A. et al. (2016) M1 and M2 macrophage recruitment during tendon regeneration induced by amniotic epithelial cell allotransplantation in ovine.
    Res Vet Sci. 105: 92-102.
  12. Corripio-miyar, Y. et al. (2017) 1,25-Dihydroxyvitamin D3 modulates the phenotype and function of Monocyte derived dendritic cells in cattle.
    BMC Vet Res. 13 (1): 390.
  13. Marzo, S. et al. (2021) Characterisation of dendritic cell frequency and phenotype in bovine afferent lymph reveals kinetic changes in costimulatory molecule expression
    Vet Immunol Immunopathol. 19 Nov: 110363.
  14. Wherry, T.L.T. et al. (2022) Effects of 1,25-Dihydroxyvitamin D3 and 25-Hydroxyvitamin D3 on PBMCs From Dairy Cattle Naturally Infected With Mycobacterium avium subsp. paratuberculosis.
    Front Vet Sci. 9: 830144.
  15. Liu, J. et al. (2020) Theileria annulata. transformation altered cell surface molecules expression and endocytic function of monocyte-derived dendritic cells.
    Ticks Tick Borne Dis. 11 (3): 101365.
  16. Russo, V. et al. (2022) Tendon Healing Response Is Dependent on Epithelial-Mesenchymal-Tendon Transition State of Amniotic Epithelial Stem Cells.
    Biomedicines. 10 (5): 1177.
  17. Stabel, J.R. et al. (2022) B cell phenotypes and maturation states in cows naturally infected with Mycobacterium avium subsp. Paratuberculosis.
    PLoS One. 17 (12): e0278313.

Flow Cytometry

UniProt
Q1JPC5

MCA2437F

1015 160235

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