CD41/CD61 antibody | 11C3
CSF1R-transgene expression is restricted to macrophages in MacReporter embryos. (A) CSF1R-mApple+ cells (red) are restricted to the lumen of primitive blood vessels in ubiquitous CAG-eGFP-expressing HH13 stage embryos (green). (B,C) Confocal analysis of transgene expression in HH21 stage CSF1R-mApple embryos indicates that transgene expression is restricted to CD45+ (B, green), CSF1R+ (C, green) cells in the mesenchyme (red arrowheads) and not CD45+ cells budding from the epithelial layer of the dorsal aorta (white arrowheads). Dotted lines mark the blood vessel (BV) lumen. Scale bars in A-C: 100 µm. (D-F) Confocal analysis of CSF1R staining (green) of CSF1R-mApple transgene-expressing cells (red) in the mesenchyme tissue of a HH29 embryo. The transgene is expressed in cells (red) that are CD45+ (D, green) and CSF1R+ (E, green), but are CD41/61− (F, green). Scale bars in D-F: 100 µm. BV, blood vessel lumen. (G) Scattered eGFP+ cells are found in the embryonic (Emb.) and extra-exbryonic (Ex-Emb) tissues of HH15 MacGreen embryos. Scale bar: 200 μm. (H-J) Colocalization of eGFP+ cells with LysoTracker Red-stained lysosomes in HH33 embryo footplate and in the interdigit region. Inset in J shows the boxed area in more detail. Scale bars in G-J: 200 µm.
From: Balic A, Garcia-Morales C, Vervelde L, Gilhooley H, Sherman A, Garceau V, Gutowska MW, Burt DW, Kaiser P, Hume DA, Sang HM.
Visualisation of chicken macrophages using transgenic reporter genes: insights into the development of the avian macrophage lineage.
Development. 2014 Aug;141(16):3255-65.
This image is from an open access article distributed under the terms of the Creative Commons Attribution License.
FITC conjugated Mouse anti Chicken CD41/61 antibody, clone 11C3 (MCA2240F) used to identify cell sub-populations in chicken intraepithlial lymphocytes by flow cytomwtry.
Image caption:
Effect of adult microbiota (AM) on NK cells in broiler chickens. (A) Gating strategy after isolation of lymphocytes from IEL to analyze NK cell subsets and activation.
From:Meijerink N, Kers JG, Velkers FC, van Haarlem DA, Lamot DM, de Oliveira JE, Smidt H, Stegeman JA, Rutten VPMG, Jansen CA.
Early Life Inoculation With Adult-Derived Microbiota Accelerates Maturation of Intestinal Microbiota and Enhances NK Cell Activation in Broiler Chickens.
Front Vet Sci. 2020 Nov 19;7:584561.
doi: 10.3389/fvets.2020.584561.
This image is from an open access article distributed under terms of a Creative Commons Attribution License.
FITC conjugated Mouse anti Chicken CD41/61, clone 11C3 (MCA2240F) used for the isolation and characterization of chicken NK cells from intraepithelial lymphocytes by flow cytometry.
Image caption:
Phenotypic characterization of intestinal NK cells in embryos and chickens. Gating strategy after isolation of lymphocytes from IEL to analyze NK cell subsets and their activation; lymphocytes were gated, within lymphocytes the live lymphocytes, therein the CD3 negative cells followed by selection of cells expressing IL-2Rα, 20E5 and 5C7 (NK cell subsets) or the CD3 and CD41/61 negative cells followed by selection of cells expressing CD107 in total NK cells and within the IL-2Rα+ and 20E5+ NK cells (A).
From: Meijerink N, van Haarlem DA, Velkers FC, Stegeman AJ, Rutten VPMG, Jansen CA.
Analysis of chicken intestinal natural killer cells, a major IEL subset during embryonic and early life.
Dev Comp Immunol. 2021 Jan;114:103857.
doi: 10.1016/j.dci.2020.103857.
This image is from an open access article distributed under terms of a Creative Commons Attribution License.
Mouse anti Chicken CD41/61, clone 11C3 (MCA2240GA) used to label chicken thrombocytes by flow cytometry.
Image caption:
Thrombocyte characteristics: EDTA blood was processed by density gradient centrifugation (A–C,G) or slow speed centrifugation (D–F,H) and stained with anti-CD45 (16-6), followed by anti-mouse-IgG2a-FITC and anti-CD41/61 (11C3) and anti-IgG1-PE (A,B,D,E,G,H) or anti-MRC1L-B (clone KUL01, IgG1) and anti-IgG1-APC (C,F–H). Numbers in scatter plots (A,D) represent 1 = thrombocytes, 2 = monocytes, and 3 = lymphocytes; FSC/SSC profile of thrombocytes (red), lymphocytes (blue), and monocytes (dark green) analyzed with common (G) or increased SSC voltage (H).
From: Citation: Härtle S, Sutton K, Vervelde L and Dalgaard TS (2024)
Delineation of chicken immune markers in the era of omics and multicolor flow cytometry.
Front. Vet. Sci. 11:1385400.
doi: 10.3389/fvets.2024.1385400
This image is from an open access article distributed under terms of a Creative Commons Attribution License.
RPE conjugated Mouse anti Chicken CD41/61, clone 11C3 (MCA2240PE) used to evaluate GPIIb-IIIa expression on chicken cells by flow cytometry.
Image caption:
(b) Flow cytometry gating strategy used for the analysis of splenocytes that underwent antibody staining for the identification of avian lymphocytes (CD4+, CD8α+, γδTCR+, CD4+γδTCR+) and antigen-presenting avian phagocytes (MRCL1-B+, MHC-II+, MRCL1-B+MHC-II+hi, MRCL1-B+MHC-II+lo). FSC-Area/FSC-Linear doublet discrimination (singlets) ensured that mostly single cells were selected for analysis. This was flowed by exclusion of dead cells using the fluorescent intercalator 7AAD, that undergoes a spectral shift upon association with DNA. Finally, forward versus side scatter (FSC vs SSC) gating is commonly used to identify cells of interest based on size and granularity (complexity). Isotype controls are also represented in the overall strategy.
From:Saint-Martin V, Guillory V, Chollot M, Fleurot I, Kut E, Roesch F, Caballero I, Helloin E, Chambellon E, Ferguson B, Velge P, Kempf F, Trapp S, Guabiraba R.
The gut microbiota and its metabolite butyrate shape metabolism and antiviral immunity along the gut-lung axis in the chicken.
Commun Biol. 2024 Sep 20;7(1):1185.
doi: 10.1038/s42003-024-06815-0.
This image is from an open access article distributed under terms of a Creative Commons Attribution License.
Filter by Application:
F IF ResetMouse anti Chicken CD41/CD61
- Product Type
- Monoclonal Antibody
- Clone
- 11C3
- Isotype
- IgG1
- Specificity
- CD41/CD61
Antibody Quality and Performance Guarantee
| Mouse anti Chicken CD41/61, clone 11C3 recognizes chicken integrin CD41/61, also known as GPIIb-IIIa. CD41/61 is a heterodimeric cell surface protein, expressed on chicken thrombocytes and cells of the thrombocyte lineage. Avian thrombocytes are nucleated equivalents of mammalian platelets playing roles in coagulation and phagocytosis. Mouse anti Chicken CD41/61 clone 11C3 is reported to induce thrombocyte activation (Lacoste-Eleaume et al. 1994). |
- Target Species
- Chicken
- Product Form
- Purified IgG - liquid
- Preparation
- Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
- 0.09% sodium azide (NaN3)
- Carrier Free
- Yes
- Immunogen
- Peripheral blood leukocytes from white leghorn chicken
- Approx. Protein Concentrations
- IgG concentration 1.0 mg/ml
- Fusion Partners
- Spleen cells from immunised BALB/c mice were fused with cells of the X63/A63-653 myeloma cell line
- Regulatory
- For research purposes only
- Guarantee
- 12 months from date of despatch
- Acknowledgements
- This antibody is licensed from INRA-INSERM
This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
| Application Name | Verified | Min Dilution | Max Dilution |
|---|---|---|---|
| Flow Cytometry |  |
1/10 | 1/50 |
| Immunofluorescence | |
||
| Immunoprecipitation | |
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
- Flow Cytometry
- Use 10μl of the suggested working dilution to label 106 cells in 100μl
Source Reference
-
Lacoste-Eleaume, A.S. et al. (1994) Biochemical and functional characterization of an avian homolog of the integrin GPIIb-IIIa present on chicken thrombocytes.
Exp Cell Res. 213 (1): 198-209.
References for CD41/CD61 antibody
-
Horiuchi, H. et al. (2004) A monoclonal antibody against chicken thrombocytes reacts with the cells of thrombocyte lineage.
J Vet Med Sci. 66 (3): 243-50. -
Corbel, C. (2002) Expression of alphaVbeta3 integrin in the chick embryo aortic endothelium.
Int J Dev Biol. 46 (6): 827-30. -
Del Cacho, E. et al. (2008) Isolation of chicken follicular dendritic cells.
J Immunol Methods. 334: 59-69. -
Del Cacho, E. et al. (2009) Avian follicular and interdigitating dendritic cells: isolation and morphologic, phenotypic, and functional analyses.
Vet Immunol Immunopathol. 129: 66-75. -
Ody, C. et al. (1999) Glycoprotein IIb-IIIa is expressed on avian multilineage hematopoietic progenitor cells.
Blood 93: 2898-906. -
Chaussé, A.M. et al. (2011) Expression of Toll-like receptor 4 and downstream effectors in selected cecal cell subpopulations of chicks resistant or susceptible to Salmonella carrier state.
Infect Immun. 79: 3445-54. -
Balic, A. et al. (2014) Visualisation of chicken macrophages using transgenic reporter genes: insights into the development of the avian macrophage lineage.
Development 141: 3255-65. -
Meijerink, N. et al. (2020) Early Life Inoculation With Adult-Derived Microbiota Accelerates Maturation of Intestinal Microbiota and Enhances NK Cell Activation in Broiler Chickens.
Front Vet Sci. 7: 584561.
View The Latest Product References
-
Meijerink, N. et al. (2021) Analysis of chicken intestinal natural killer cells, a major IEL subset during embryonic and early life.
Dev Comp Immunol. 114: 103857. -
Meijerink, N. et al. (2021) Glucose Oligosaccharide and Long-Chain Glucomannan Feed Additives Induce Enhanced Activation of Intraepithelial NK Cells and Relative Abundance of Commensal Lactic Acid Bacteria in Broiler Chickens.
Vet Sci. 8 (6): 110. -
Härtle, S. et al. (2024) Delineation of chicken immune markers in the era of omics and multicolor flow cytometry
Frontiers in Veterinary Science. 23 May [Epub ahead of print]. -
Saint-Martin, V. et al. (2024) The gut microbiota and its metabolite butyrate shape metabolism and antiviral immunity along the gut-lung axis in the chicken.
Commun Biol. 7 (1): 1185.
- Synonyms
- GPIIb-IIIa
MCA2240GA
152979
If you cannot find the batch/lot you are looking for please contact our technical support team for assistance.
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