MHC Class II Monomorphic antibody | 21-1A6
|Mouse anti Chicken MHC Class II (monomorphic) antibody, clone 21-1A6, recognizes a monomorphic determinant on the chicken B-L molecule, the chicken class II major histocompatibility complex (MHC).
The level of B-L expression is reported to increase during the bursal phase of B cell differentiation (Veromaa et al. 1988).
- Target Species
- Product Form
- Purified IgG - liquid
- Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
- 0.09% sodium azide (NaN3)
- Carrier Free
- Chicken bursa cells
- Approx. Protein Concentrations
- IgG concentration 1.0 mg/ml
- Fusion Partners
- Spleen cells from immunized Balb/c mice were fused with cells of the mouse NS-1 myeloma cell line
- For research purposes only
- 12 months from date of despatch
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.
|Application Name||Verified||Min Dilution||Max Dilution|
|Immunohistology - Frozen|
- Flow Cytometry
- Use 10μl of the suggested working dilution to label 106 cells in 100μl
References for MHC Class II Monomorphic antibody
Veromaa, T. et al. (1988) Expression of B-L and Bu-1 antigens in chickens bursectomized at 60 h of incubation.
Eur J Immunol. 18 (2): 225-30.
Vainio, O. et al. (1988) Antigen-presenting cell-T cell interaction in the chicken is MHC class II antigen restricted.
J Immunol. 140 (9): 2864-8.
Petkov, D.I. et al. (2009) Identification and characterization of two distinct bursal B-cell subpopulations following infectious bursal disease virus infection of White Leghorn chickens.
Avian Dis. 53 (3): 347-55.
Silva, A.B. et al. (2008) Functional analysis of neuropeptides in avian thymocyte development.
Dev Comp Immunol. 32 (4): 410-20.
Pavlova, S.P. et al. (2010) In vitro and in vivo characterization of glycoprotein C-deleted infectious laryngotracheitis virus.
J Gen Virol. 91 (Pt 4): 847-57.
Wattrang, E. (2009) Phosphorothioate oligodeoxyribonucleotides induce in vitro proliferation of chicken B-cells.
Vet Immunol Immunopathol. 131 (3-4): 218-28.
Kamble, N.M. et al. (2016) Interaction of a live attenuated Salmonella gallinarum vaccine candidate with chicken bone marrow-derived dendritic cells.
Avian Pathol. 45 (2): 235-43.
Jarosz, Ł. et al. et al. (2016) Effects of feed supplementation with glycine chelate and iron sulfate on selected parameters of cell-mediated immune response in broiler chickens.
Res Vet Sci. 107: 68-74.
View The Latest Product References
Eren, U. et al. (2016) The several elements of intestinal innate immune system at the beginning of the life of broiler chicks.
Microsc Res Tech. 79 (7): 604-14.
Kamble, N.M. et al. (2016) Activation of chicken bone marrow-derived dendritic cells induced by a Salmonella Enteritidis ghost vaccine candidate.
Poult Sci. 95 (10): 2274-80.
Jarosz, Ł.S. et al. (2018) The effect of feed supplementation with a copper-glycine chelate and copper sulphate on selected humoral and cell-mediated immune parameters, plasma superoxide dismutase activity, ceruloplasmin and cytokine concentration in broiler chickens.
J Anim Physiol Anim Nutr (Berl). 102 (1): e326-e336.
Shojadoost, B. et al. (2019) Interactions between lactobacilli and chicken macrophages induce antiviral responses against avian influenza virus.
Res Vet Sci. 125: 441-50.
Garrido, D. et al. (2018) The role of type I interferons (IFNs) in the regulation of chicken macrophage inflammatory response to bacterial challenge.
Dev Comp Immunol. 86: 156-70.
Yildiz, M. et al. (2019) Histological and immunohistochemical studies of the proximal caecum and caecal tonsils of quail (Coturnix coturnix japonica).
Anat Histol Embryol. 48 (5): 476-85.
Eren, U. et al. (2022) TLR2 and TLR4 molecules and antigen-presenting cell compositions in cecal tonsils of broiler chicks (Gallus gallus domesticus.) in the first two weeks of the post-hatch period.
Anat Histol Embryol. 51 (1): 125-135.
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