Monocytes/Macrophages antibody | KUL01
Mouse anti Chicken monocytes/macrophages antibody, clone KUL01 (MCA5770) used for flow cytometry studies.
Image caption:
(C) Flow cytometric analysis of reporter transgene expression in KUL01+/CSF1R+ blood monocytes in a non-transgenic chicken, G1 cockerel MAA1-8:23 and representative G2 progeny from (B) that have either a full length or a partially-deleted insert.
From: Balic A, Garcia-Morales C, Vervelde L, Gilhooley H, Sherman A, Garceau V, Gutowska MW, Burt DW, Kaiser P, Hume DA, Sang HM.
Visualisation of chicken macrophages using transgenic reporter genes: insights into the development of the avian macrophage lineage.
Development. 2014 Aug;141(16):3255-65.
This image is from an open access article distributed under terms of a Creative Commons Attribution License.
Mouse anti Chicken monocytes/macrophages antibody, clone KUL01 (MCA5770) used for Immunohistochemistry.
Image caption:
Immunohistochemical evidence of infiltration by T-cells (CD3+), B-cells (chB6+) and macrophages (Kul01+). Immunophenotyping identified the majority of infiltrating lymphocytes as being CD3-positive T-cells (A). They show a characteristic multifocal distribution pattern. With similar spatial characteristics, chB6-positive B-cells comprise the second largest fraction of infiltrating cells (B). Compared to the lymphocytes, the density of Kul01-positive macrophages appears much lower (C). The arrows point to immunopositive cells. All sections were prepared from the same region of the sciatic nerve. The orientation of the nerve fibres (NF) is indicated. Scale bar = 35μm.
From: Bader SR, Kothlow S, Trapp S, Schwarz SC, Philipp HC, Weigend S, Sharifi AR, Preisinger R, Schmahl W, Kaspers B, Matiasek K.
Acute paretic syndrome in juvenile White Leghorn chickens resembles late stages of acute inflammatory demyelinating polyneuropathies in humans.
J Neuroinflammation. 2010 Jan 28;7:7.
This image is from an open access article distributed under terms of a Creative Commons Attribution License.
Mouse anti Chicken Monocytes/Macrophages, clone KUL01 (MCA5770) used to label macrophages in chicken caecal sections by immunofluorescence.
Image caption:
Localization of KUL01+ cells during E. tenella infection in caeca from conventional and GF chickens. Imbedded frozen OCT caecal sections were stained with DAPI (Blue) and KUL01-Alexa 594 (Red). Non-infected and 7 days pi conventional (A and C), 7 days pi GF (B and D), 7 days pi GF chickens receiving a microbiota from healthy conventional chickens at 4 days pi (E). Magnification 5X and scale bar indicated 100μm
From: Tomal F, Sausset A, Le Vern Y, Sedano L, Techer C, Lacroix-Lamandé S, Laurent F, Silvestre A, Bussière FI.
Microbiota promotes recruitment and pro-inflammatory response of caecal macrophages during E. tenella infection.
Gut Pathog. 2023 Dec 14;15(1):65.
doi: 10.1186/s13099-023-00591-8.
This image is from an open access article distributed under terms of a Creative Commons Attribution License.
Mouse anti Chicken Monocytes/Macrophages, clone KUL01 (MCA5770) used to label macrophages in chicken lung sections.
Image caption:
Immunostaining of PCLS. PCLS (200–300 μm) prepared from PA12 SPF chickens 1 day post slice were stained with a panel of antibodies and examined by confocal microscopy. Nuclei are represented in blue (DAPI). Longitudinal section showing MCRL1-B+ phagocytes (green) (C) within the lung parenchyma (C, panels i and ii), especially close to the septae. The white box (C, panel i) represents the area digitally enlarged (C, panel ii).
From: Bryson KJ, Garrido D, Esposito M, McLachlan G, Digard P, Schouler C, Guabiraba R, Trapp S, Vervelde L.
Precision cut lung slices: a novel versatile tool to examine host-pathogen interaction in the chicken lung.
Vet Res. 2020 Jan 10;51(1):2.
doi: 10.1186/s13567-019-0733-0.
This image is from an open access article distributed under terms of a Creative Commons Attribution License.
Mouse anti Chicken Monocytes/Macrophages, clone KUL01 (MCA5770) used to label chicken monocytes and macrophages by flow cytometry following conjugation to allophycocyanin.
Image caption:
(b) Flow cytometry gating strategy used for the analysis of splenocytes that underwent antibody staining for the identification of avian lymphocytes (CD4+, CD8α+, γδTCR+, CD4+γδTCR+) and antigen-presenting avian phagocytes (MRCL1-B+, MHC-II+, MRCL1-B+MHC-II+hi, MRCL1-B+MHC-II+lo). FSC-Area/FSC-Linear doublet discrimination (singlets) ensured that mostly single cells were selected for analysis. This was flowed by exclusion of dead cells using the fluorescent intercalator 7AAD, that undergoes a spectral shift upon association with DNA. Finally, forward versus side scatter (FSC vs SSC) gating is commonly used to identify cells of interest based on size and granularity (complexity). Isotype controls are also represented in the overall strategy.
From:Saint-Martin V, Guillory V, Chollot M, Fleurot I, Kut E, Roesch F, Caballero I, Helloin E, Chambellon E, Ferguson B, Velge P, Kempf F, Trapp S, Guabiraba R.
The gut microbiota and its metabolite butyrate shape metabolism and antiviral immunity along the gut-lung axis in the chicken.
Commun Biol. 2024 Sep 20;7(1):1185.
doi: 10.1038/s42003-024-06815-0.
This image is from an open access article distributed under terms of a Creative Commons Attribution License.
Filter by Application:
F P IF ResetMouse anti Chicken Monocytes/Macrophages
- Product Type
- Monoclonal Antibody
- Clone
- KUL01
- Isotype
- IgG1
- Specificity
- Monocytes/Macrophages
Antibody Quality and Performance Guarantee
| Mouse anti Chicken Monocytes/Macrophages, clone KUL01 recognises chicken monocytes and macrophages. Mouse anti Chicken monocytes/ macrophages, clone KUL01 also detects interdigitating cells and activated microglia but does not recognise Bu1+ve B-cells or CD3 +ve T-lymphocytes. Mouse anti Chicken monocytes/ macrophages, clone KUL01 has subsequently been demonstrated to recognize chicken MRC1L-B, a homologue of the mammalian mannose receptor MRC1 (Staines et al. 2014). Five papalogous genes have been identified in the chicken genome, named MRC1L-A-E. Mouse anti Chicken monocytes / macrophages antibody only recognizes the MRC1L-B gene product.(Staines et al. 2014). |
- Target Species
- Chicken
- Product Form
- Purified IgG - liquid
- Preparation
- Purified IgG prepared by ion exchange chromatography from tissue culture supernatant
- Buffer Solution
- Borate buffered saline.
- Preservative Stabilisers
- <0.1% Sodium Azide (NaN3)
- Immunogen
- Chicken peripheral blood mononuclear leukocytes (PBML).
- Approx. Protein Concentrations
- IgG concentration 0.5mg/ml
- Fusion Partners
- Spleen cells from Balb/c mice immunized with chicken PBML were fused with cells from the Sp2/0-Ag14 mouse myeloma cell line.
- Regulatory
- For research purposes only
- Guarantee
- 12 months from date of despatch
This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
| Application Name | Verified | Min Dilution | Max Dilution |
|---|---|---|---|
| Flow Cytometry |  |
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| Immunohistology - Frozen | |
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| Immunohistology - Paraffin 1 | |
- 1This product requires protein digestion pre-treatment of paraffin sections e.g. trypsin or pronase.
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
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| Description | Goat anti Mouse IgG (H/L):FITC (Multi Species Adsorbed) | ||||||
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| Description | Goat anti Mouse IgG (H/L):HRP (Multi Species Adsorbed) | ||||||
| Rabbit F(ab')2 anti Mouse IgG:RPE | STAR12A | F | 1 ml |
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| Rabbit F(ab')2 anti Mouse IgG:FITC | STAR9B | F | 1 mg |
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Source Reference
-
Mast, J. et al. (1998) Characterisation of chicken monocytes, macrophages and interdigitating cells by the monoclonal antibody KUL01.
Vet Immunol Immunopathol. 61 (2-4): 343-57.
References for Monocytes/Macrophages antibody
-
Wigley, P. et al. (2001) Salmonella enterica serovar Pullorum persists in splenic macrophages and in the reproductive tract during persistent, disease-free carriage in chickens.
Infect Immun. 69 (12): 7873-9. -
Balic, A. et al. (2014) Visualisation of chicken macrophages using transgenic reporter genes: insights into the development of the avian macrophage lineage.
Development. 141: 3255-65. -
Garcia-Morales, C. et al. (2014) Production and characterisation of a monoclonal antibody that recognises the chicken CSF1 receptor and confirms that expression is restricted to macrophage-lineage cells.
Dev Comp Immunol. 42 (2): 278-85. -
Guabiraba, R. et al. (2017) Unveiling the participation of avian kinin ornithokinin and its receptors in the chicken inflammatory response.
Vet Immunol Immunopathol. 188: 34-47. -
Tomal, F. et al. (2023) Microbiota promotes recruitment and pro-inflammatory response of caecal macrophages during E. tenella infection.
Gut Pathog. 15 (1): 65. -
Alber, A. et al. (2019) Dose-dependent differential resistance of inbred chicken lines to avian pathogenic Escherichia coli challenge.
Avian Pathol. 48 (2): 157-67. -
Farsang, A. et al. (2019) Avian coronavirus infection induces mannose-binding lectin production in dendritic cell precursors of chicken lymphoid organs.
Acta Vet Hung. 67 (2): 183-96. -
Bryson, K.J. et al. (2020) Precision cut lung slices: a novel versatile tool to examine host-pathogen interaction in the chicken lung.
Vet Res. 51 (1): 2.
View The Latest Product References
-
Saint-Martin, V. et al. (2024) The gut microbiota and its metabolite butyrate shape metabolism and antiviral immunity along the gut-lung axis in the chicken.
Commun Biol. 7 (1): 1185.
- Synonyms
- MRC1L-B
- RRID
- AB_10841619
- UniProt
- M1XGZ4
MCA5770
150571
156457
166899
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