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Monocytes/Macrophages antibody | KUL01

Mouse anti Chicken Monocytes/Macrophages

Product Type
Monoclonal Antibody

Product Code Applications Pack Size List Price Your Price Qty
Datasheet Datasheet Datasheet
SDS Safety Datasheet SDS
C F P * 0.25 mg loader
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Mouse anti Chicken Monocytes/Macrophages, clone KUL01 recognises chicken monocytes and macrophages. Mouse anti Chicken monocytes/ macrophages, clone KUL01 also detects interdigitating cells and activated microglia but does not recognise Bu1+ve B-cells or CD3 +ve T-lymphocytes.

Mouse anti Chicken monocytes/ macrophages, clone KUL01 has subsequently been demonstrated to recognize chicken MRC1L-B, a homologue of the mammalian mannose receptor MRC1 (Staines et al. 2014). Five papalogous genes have been identified in the chicken genome, named MRC1L-A-E. Mouse anti Chicken monocytes / macrophages antibody only recognizes the MRC1L-B gene product.(Staines et al. 2014).

Target Species
Product Form
Purified IgG - liquid
Purified IgG prepared by ion exchange chromatography from tissue culture supernatant
Buffer Solution
Borate buffered saline.
Preservative Stabilisers
<0.1% Sodium Azide (NaN3)
Chicken peripheral blood mononuclear leukocytes (PBML).
Approx. Protein Concentrations
IgG concentration 0.5mg/ml
Fusion Partners
Spleen cells from Balb/c mice immunized with chicken PBML were fused with cells from the Sp2/0-Ag14 mouse myeloma cell line.
For research purposes only
12 months from date of despatch

This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.

Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Flow Cytometry
Immunohistology - Frozen
Immunohistology - Paraffin 1
  1. 1This product requires protein digestion pre-treatment of paraffin sections e.g. trypsin or pronase.
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.

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Source Reference

  1. Mast, J. et al. (1998) Characterisation of chicken monocytes, macrophages and interdigitating cells by the monoclonal antibody KUL01.
    Vet Immunol Immunopathol. 61 (2-4): 343-57.

References for Monocytes/Macrophages antibody

  1. Wigley, P. et al. (2001) Salmonella enterica serovar Pullorum persists in splenic macrophages and in the reproductive tract during persistent, disease-free carriage in chickens.
    Infect Immun. 69 (12): 7873-9.
  2. Balic, A. et al. (2014) Visualisation of chicken macrophages using transgenic reporter genes: insights into the development of the avian macrophage lineage.
    Development. 141: 3255-65.
  3. Garcia-Morales, C. et al. (2014) Production and characterisation of a monoclonal antibody that recognises the chicken CSF1 receptor and confirms that expression is restricted to macrophage-lineage cells.
    Dev Comp Immunol. 42 (2): 278-85.
  4. Guabiraba, R. et al. (2017) Unveiling the participation of avian kinin ornithokinin and its receptors in the chicken inflammatory response.
    Vet Immunol Immunopathol. 188: 34-47.
  5. Tomal, F. et al. (2023) Microbiota promotes recruitment and pro-inflammatory response of caecal macrophages during E. tenella infection.
    Gut Pathog. 15 (1): 65.
  6. Alber, A. et al. (2019) Dose-dependent differential resistance of inbred chicken lines to avian pathogenic Escherichia coli challenge.
    Avian Pathol. 48 (2): 157-67.
  7. Farsang, A. et al. (2019) Avian coronavirus infection induces mannose-binding lectin production in dendritic cell precursors of chicken lymphoid organs.
    Acta Vet Hung. 67 (2): 183-96.
  8. Bryson, K.J. et al. (2020) Precision cut lung slices: a novel versatile tool to examine host-pathogen interaction in the chicken lung.
    Vet Res. 51 (1): 2.
  9. View The Latest Product References



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