CSF1R antibody | ROS-AV170







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- Product Type
- Monoclonal Antibody
- Clone
- ROS-AV170
- Isotype
- IgG1
- Specificity
- CSF1R
Mouse anti Chicken CSF1R, clone ROS-AV170, recognizes the chicken homologue of human CSF1R, also known as CD115, CSF-1R, M-CSF receptor and c-fms. CSF1R is a single pass type 1 transmembrane protein belonging to the type III protein kinase family and is the cell surface receptor for both CSF1 and IL-34. Evidence from comparative sequence analysis across a range of vertebrate species indicates that CSF1 and IL-34 interact with distinct regions of CSF1R (Garceau et al. 2010). CSF1R cell surface expression appears to be one of the earliest events in macrophage lineage cells (Garceau et al. 2010).Mouse anti Chicken CSF1R, clone ROS-AV170 is a monoclonal antibody that has been generated to recognize chicken CSF1R . Clone ROS-AV170 has been shown to recognize the CSF1R antigen in some, but not all closely related avian species. Mouse anti Chicken CSF1R, clone ROS-AV170 is an excellent marker for chicken macrophages. |
- Target Species
- Chicken
- Species Cross-Reactivity
-
Target Species Cross Reactivity Goose Grey partridge Guinea Fowl Duck Quail Turkey Mouse Rat - N.B. Antibody reactivity and working conditions may vary between species.
- Product Form
- Purified IgG - liquid
- Preparation
- Purified IgG prepared by affinity chromatography on Protein A from tissue culture supernatant
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
- 0.09% Sodium Azide (NaN3)
- Carrier Free
- Yes
- Immunogen
- Purified ChCSF1R-Fc fusion protein
- Approx. Protein Concentrations
- IgG concentration 1.0 mg/ml
- Fusion Partners
- Spleen cells from immunized Balb/c mice were fused with cells of the Sp2/0-Ag14 myeloma cell line
- Regulatory
- For research purposes only
- Guarantee
- 12 months from date of despatch
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.
Application Name | Verified | Min Dilution | Max Dilution |
---|---|---|---|
Flow Cytometry | ![]() |
1/50 | 1/200 |
Immunofluorescence | ![]() |
||
Immunohistology - Frozen | ![]() |
- Flow Cytometry
- Use 10ul of the suggested working dilution to label 106 cells in 100ul
Description | Product Code | Applications | Pack Size | List Price | Your Price | Quantity | |
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Human anti Chicken CD25:FITC | HCA173F | F | 0.1 mg |
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Description | Human anti Chicken CD25:FITC | ||||||
Mouse anti Chicken Bu-1A:FITC | MCA2170F | F | 0.1 mg |
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List Price | Your Price | ||||||
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Description | Mouse anti Chicken Bu-1A:FITC | ||||||
Mouse anti Chicken CD45:RPE | MCA2413PE | F | 100 Tests | Log in | |||
List Price | Your Price | ||||||
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Description | Mouse anti Chicken CD45:RPE | ||||||
Mouse anti Chicken CD14 | MCA5926GA | E WB | 0.1 mg |
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List Price | Your Price | ||||||
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Description | Mouse anti Chicken CD14 |
Source Reference
-
Garcia-Morales, C. et al. (2014) Production and characterisation of a monoclonal antibody that recognises the chicken CSF1 receptor and confirms that expression is restricted to macrophage-lineage cells.
Dev Comp Immunol 42:278-85.
References for CSF1R antibody
-
Garceau, V. et al. (2015) The development and maintenance of the mononuclear phagocyte system of the chick is controlled by signals from the macrophage colony-stimulating factor receptor.
BMC Biol. 13:12. -
Nagy, N. et al. (2016) Avian dendritic cells: Phenotype and ontogeny in lymphoid organs.
Dev Comp Immunol. 58: 47-59. -
Härtle, S. et al. (2024) Delineation of chicken immune markers in the era of omics and multicolor flow cytometry
Frontiers in Veterinary Science. 23 May [Epub ahead of print].
Further Reading
-
Garceau, V. et al. (2010) Pivotal Advance: Avian colony-stimulating factor 1 (CSF-1), interleukin-34 (IL-34), and CSF-1 receptor genes and gene products.
J Leukoc Biol. 87:753-64.
- Synonyms
- CD115
MCA5956GA


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