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Bcl-2 Antibody | 100

Bcl-2 Antibody | 100 gallery image 1

Staining of paraffin embedded human tonsil with Mouse anti Human Bcl-2 (MCA1550)

Bcl-2 Antibody | 100 gallery image 2

Western blot analysis of Jurkat T cell leukemic whole cell lysate probed with Mouse anti Human Bcl-2 antibody (MCA1550) followed by HRP conjugated Goat anti Mouse IgG secondary antibody, visualized using chemiluminescence

  • Bcl-2 Antibody | 100 thumbnail image 1
  • Bcl-2 Antibody | 100 thumbnail image 2
  • Mouse anti Human Bcl-2
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  • Product Type
    Monoclonal Antibody
  • Clone
  • Isotype
1 Formats Available
    Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
    MCA1550C, F *, IP, P *, WBdatasheet pdfdatasheet pdf0.1 mg
    Secondary Antibodies
    Negative Isotype Controls
    Useful Reagents
    Positive Controls
    Histology Controls
    More Images
    • Mouse anti Human Bcl-2 antibody, clone 100 recognizes the human Apoptosis regulator Bcl-2 oncoprotein, a 239 amino acid ~25kDa integral single pass membrane protein containing 4 BH motifs which lies within the cell rather than on the cell surface. The protein is localised in the outer mitochondrial membrane and plays a role in the inhibition of apoptosis.

      Mouse anti Human Bcl-2, clone 100 reacts with small B lymphocytes in the mantle zone and many cells within T cell areas. Very few cells in germinal centres are stained. In the thymus many cells in the medulla are stained but the cortex shows weak or negative staining. In non-haematopoietic tissues few cells are stained (Krajewski et al. 1995). The antibody reacts with neoplastic cells of follicular lymphoma and anaplastic large cell lymphoma (Reed 1997).

      Mouse anti human Bcl-2, clone 100 has been successfully used to demonstrate Bcl-2 in formalin fixed, paraffin embedded tissues of radioresistant squamous cell carcinoma by immunohistochemistry (Condon et al. 2002).
    • This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.

    • Application NameYesNoMin DilutionMax Dilution
      Immunohistology - Frozen1/201/40
      Western Blotting
      Flow Cytometry(1)1/501/100
      Immunohistology - Paraffin(2)1/201/40
      Membrane permeabilisation is required for this application. Bio-Rad recommends the use of Leucoperm (Product Code BUF09) for this purpose.
      This product requires antigen retrieval using heat treatment prior to staining of paraffin sections.

      Sodium citrate buffer pH 6.0 is recommended for this purpose.

    • Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
    • Technical Advice
    • Recommended Protocol
    • Flow Cytometry
      Use 10ul of the suggested working dilution to label 1x106 cells in 100ul.
    • ELISA
    • Immunohistology
    • Histology Positive Control Tissue
      Human tonsil
    • Immunofluorescence
    • Western Blotting
    • Instructions For Use

    Additional Bcl-2 Antibody Formats

    Formats Clone Applications Sizes available
    Bcl-2 Antibody : Purified 100 C, F *, IP, P *, WB 0.1 mg
    • Copyright © 2016 Bio-Rad

    Recommended Secondary Antibody

      DescriptionProduct CodePack SizeApplicationsList PriceQuantity
      Goat anti Mouse IgG (H/L):Alk. Phos. (Multi Species Adsorbed)STAR117A0.5 mgE, WB
      Goat anti Mouse IgG/A/M:Alk. Phos.STAR87A1 mgC, E, WB
      Goat anti Mouse IgG (H/L):DyLight®488 (Multi Species Adsorbed)STAR117D488GA0.1 mgF, IF
      Goat anti Mouse IgG (H/L):DyLight®549 (Multi Species Adsorbed)STAR117D549GA0.1 mgF, IF
      Goat anti Mouse IgG (H/L):DyLight®649 (Multi Species Adsorbed)STAR117D649GA0.1 mgF, IF
      Goat anti Mouse IgG (H/L):DyLight®680 (Multi Species Adsorbed)STAR117D680GA0.1 mgF, WB
      Goat anti Mouse IgG (H/L):DyLight®800 (Multi Species Adsorbed)STAR117D800GA0.1 mgF, IF, WB
      Rabbit F(ab')2 anti Mouse IgG:Dylight®800STAR8D800GA0.1 mgF, IF, WB
      Goat anti Mouse IgG (H/L):FITC (Multi Species Adsorbed)STAR117F0.5 mgF
      Goat anti Mouse IgG:FITC (Rat Adsorbed)STAR700.5 mgF
      Goat anti Mouse IgG (Fc):FITCSTAR120F1 mgC, F
      Rabbit F(ab')2 anti Mouse IgG:FITCSTAR9B1 mgF
      Human anti Mouse IgG1:HRPHCA036P0.1 mgE
      Goat anti Mouse IgG (H/L):HRP (Multi Species Adsorbed)STAR117P0.5 mgE, WB
      Goat anti Mouse IgG:HRP (Rat Adsorbed)STAR770.5 mgC, E, P
      Goat anti Mouse IgG (Fc):HRPSTAR120P1 mgE, WB
      Rabbit F(ab')2 anti Mouse IgG:HRP (Human Adsorbed)STAR13B1 mgC, E, P, RE, WB
      Goat anti Mouse IgG/A/M:HRP (Human Adsorbed)STAR87P1 mgE
      Rabbit F(ab')2 anti Mouse IgG:RPESTAR12A1 mlF
      Goat anti Mouse IgG:RPE (Rat Adsorbed)STAR761 mlF

      Recommended Negative Isotype Control

        DescriptionProduct CodePack SizeApplicationsList PriceQuantity
        Mouse IgG1 Negative ControlMCA928100 TestsF

        Useful Reagents

          Recommended Positive Controls

            Histology Controls

              Human tonsil
                • Product Images

                • Bcl-2 Antibody | 100 thumbnail image 1
                • Bcl-2 Antibody | 100 thumbnail image 2
              • Application NameReference Images
                Flow Cytometry
                Western Blotting


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