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Mouse IgG2b Negative Control antibody

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Mouse IgG2b Negative Control Antibody thumbnail image 1 Mouse IgG2b Negative Control Antibody thumbnail image 2 Mouse IgG2b Negative Control Antibody thumbnail image 3 Mouse IgG2b Negative Control Antibody thumbnail image 4 Mouse IgG2b Negative Control Antibody thumbnail image 5 Mouse IgG2b Negative Control Antibody thumbnail image 6 Mouse IgG2b Negative Control Antibody thumbnail image 7 Mouse IgG2b Negative Control Antibody thumbnail image 8 Mouse IgG2b Negative Control Antibody thumbnail image 9 Mouse IgG2b Negative Control Antibody thumbnail image 10 Mouse IgG2b Negative Control Antibody thumbnail image 11 Mouse IgG2b Negative Control Antibody thumbnail image 12 Mouse IgG2b Negative Control Antibody thumbnail image 13
Mouse IgG2b Negative Control Antibody gallery image 1
Alexa Fluor 647 conjugated Mouse anti Rat CD63 (MCA4754A647) and FITC conjugated Mouse IgG1 isotype control (MCA1209F). Figure B. Alexa Fluor 647 conjugated Mouse anti Rat CD63 (MCA4754F) and FITC conjugated Mouse anti Rat CD11b (MCA619F). All experiments performed on rat peritoneal macrophages gated on live, single cells in the presence of 10% rat serum. Data acquired on the ZE5 Cell Analyzer.
Mouse IgG2b Negative Control Antibody gallery image 2
Figure A. FITC conjugated Mouse anti Human CD19 (MCA2495F) and A647 conjugated Mouse IgG2b isotype control (MCA691A647). Figure B. FITC conjugated Mouse anti Human CD19 (MCA2495F) and A647 conjugated Mouse anti Human CD20 (MCA1710A647). All experiments performed on human Peripheral blood lymphocytes in the presence of human SeroBlock (BUF070A).
Mouse IgG2b Negative Control Antibody gallery image 3
Figure A. FITC conjugated Mouse anti Human CD14 (MCA596F) and RPE conjugated Mouse IgG2b isotype control (MCA691PE). Figure B. FITC conjugated Mouse anti Human CD14 (MCA596F) and RPE conjugated Mouse anti Human CD9 (MCA469PE). All experiments performed on human peripheral blood mononuclear cells in the presence of human SeroBlock (BUF070A).
Mouse IgG2b Negative Control Antibody gallery image 4
Figure A. RPE conjugated Mouse anti Porcine CD4a (MCA1749PE) and FITC conjugated mouse IgG2b isotype control (MCA691F). Figure B. RPE conjugated Mouse anti Porcine CD4a (MCA1749PE) and FITC conjugated Mouse anti Porcine CD5 (MCA2307F). All experiments performed on red cell lysed porcine blood gated on mononuclear cells.
Mouse IgG2b Negative Control Antibody gallery image 5
Figure A. Purified Mouse anti Porcine CD25 (MCA1736GA) detected with Goat anti Mouse IgG1 PE (STAR132PE) and Mouse IgG2b FITC isotype control (MCA691F). Figure B. Purified Mouse anti Porcine CD25 (MCA1736GA) detected with goat anti mouse IgG1 PE (STAR132PE) and Mouse anti Porcine CD4a FITC (MCA1749F). All experiments performed on red cell lysed porcine blood gated on mononuclear cells.
Mouse IgG2b Negative Control Antibody gallery image 6
Figure A. Purified Mouse anti Porcine CD11R1 (MCA1220) detected with Goat anti Mouse IgG PE (STAR132PE) and Mouse IgG2b FITC isotype control (MCA691F). Figure B. Purified Mouse anti Porcine CD11R1 (MCA1220) detected with Goat anti Mouse IgG1 PE (STAR132PE) and Mouse anti Porcine CD11a FITC (MCA2308F). All experiments performed on red cell lysed porcine blood gated on mononuclear cells.
Mouse IgG2b Negative Control Antibody gallery image 7
Figure A. RPE conjugated Mouse anti Porcine CD27 (MCA5973PE) and FITC conjugated Mouse IgG2b isotype control (MCA691F). Figure B. RPE conjugated Mouse anti Porcine CD27 (MCA5973PE) and FITC conjugated Mouse anti Porcine CD18a (MCA1972F). All experiments performed on red cell lysed porcine blood gated on mononuclear cells.
Mouse IgG2b Negative Control Antibody gallery image 8
Figure A. Purified Mouse anti Porcine CD6 (MCA1221GA) detected with Goat anti Mouse IgG2a PE (STAR133PE) and mouse IgG2b FITC isotype control (MCA691F). Figure B. Purified Mouse anti Porcine CD6 (MCA1221GA) detected with Goat anti Mouse IgG2a PE (STAR133PE) and Mouse anti Porcine CD45 FITC (MCA1222F). All experiments performed on red cell lysed porcine blood gated on mononuclear cells.
Mouse IgG2b Negative Control Antibody gallery image 9
Figure A. FITC conjugated mouse anti bovine CD40 (MCA2431F) and purified mouse IgG2b isotype control (MCA691) detected with RPE conjugated goat anti mouse IgG2b (STAR134PE). Figure B. FITC conjugated mouse anti bovine CD40 (MCA2431F) and purified Mouse anti Bovine CD1w3 detected with RPE conjugated goat anti mouse IgG2b (MCASTAR134PE). All experiments performed on red cell lysed bovine blood gated on mononuclear cells.
Mouse IgG2b Negative Control Antibody gallery image 10
Figure A. RPE conjugated Mouse anti Bovine CD8b (MCA1654PE) and FITC conjugated Mouse IgG2b isotype control (MCA691F). Figure B. RPE conjugated Mouse anti Bovine CD8b (MCA1654PE) and FITC conjugated Mouse anti Bovine CD21 (MCA5953F). All experiments performed on red cell lysed bovine blood gated on mononuclear cells.
Mouse IgG2b Negative Control Antibody gallery image 11
Figure A. Purified Mouse anti Bovine CD13 (MCA2338GA) detected with Goat anti mouse IgG1 RPE (STAR132PE) and FITC conjugated Mouse IgG2b isotype control (MCA691F). Figure B. Purified Mouse anti Bovine CD13 (MCA2338GA) detected with Goat anti Mouse IgG1 RPE (STAR132PE) and FITC conjugated Mouse anti Bovine CD11b (MCA1425F). All experiments performed on red cell lysed bovine blood gated on mononuclear cells.
Mouse IgG2b Negative Control Antibody gallery image 12
Figure A. Alexa Fluor 647 conjugated mouse anti porcine CD45 (MCA1222A647) and FITC conjugated mouse IgG2b isotype control (MCA691F). Figure B. Alexa Fluor 647 conjugated mouse anti porcine CD45 (MCA1222A647) and FITC conjugated mouse anti porcine CD14 (MCA1218F). All experiments performed on red cell lysed porcine blood gated on mononuclear cells.
Mouse IgG2b Negative Control Antibody gallery image 13
Published customer image:
Fitc conjugated Mouse IgG2b Negative Control antibody (MCA691F) used as an isotype matched control for CD14 staining of porcine monocytes by flow cytometry.
Image caption:
Morphological appearance of porcine monocytes and C. difficile vegetative cells and spores. (A) FACS analysis of uninfected porcine monocytes showing a SSc high/CD14high phenotype (large cells expressing CD14) typical of monocytes.

From: Lawrence, J.; Barrow, P.; Foster, N.
Porcine Monocyte DNA Traps Formed during Infection with Pathogenic Clostridioides difficile Strains.
Pathogens 2024, 13, 228.
doi: 10.3390/pathogens13030228
This image is from an open access article distributed under terms of a Creative Commons Attribution License.

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Mouse IgG2b Negative Control:Alexa Fluor® 488

Product Type
Negative/Isotype Control
Isotype
IgG2b
Specificity
Mouse IgG2b Negative Control

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MCA691A488
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Mouse IgG2b Negative Control is negative on all human cells and cell lines tested. This antibody recognises a rat cell surface marker, and therefore cannot be used as a negative control in this species.

Mouse IgG2b Negative Control is also suitable for use as a negative control with bovine, ovine, porcine, canine and guinea-pig tissues.

Target Species
Negative Control
Product Form
Purified IgG conjugated to Alexa Fluor® 488 - liquid
Preparation
Purified IgG prepared by affinity chromatography on Protein A from tissue culture supernatant
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.09%Sodium Azide
1%Bovine Serum Albumin
Approx. Protein Concentrations
IgG concentration 0.05 mg/ml
Max Ex/Em
Fluorophore Excitation Max (nm) Emission Max (nm)
Alexa Fluor®488 495 519
Regulatory
For research purposes only
Guarantee
12 months from date of despatch
Acknowledgements
This product is provided under an intellectual property licence from Life Technologies Corporation. The transfer of this product is contingent on the buyer using the purchase product solely in research, excluding contract research or any fee for service research, and the buyer must not sell or otherwise transfer this product or its components for (a) diagnostic, therapeutic or prophylactic purposes; (b) testing, analysis or screening services, or information in return for compensation on a per-test basis; (c) manufacturing or quality assurance or quality control, or (d) resale, whether or not resold for use in research. For information on purchasing a license to this product for purposes other than as described above, contact Life Technologies Corporation, 5791 Van Allen Way, Carlsbad CA 92008 USA or outlicensing@thermofisher.com

This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.

Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended. This product is photosensitive and should be protected from light.

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Flow Cytometry *
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only.
Flow Cytometry
Use 10ul of the suggested working dilution to label 106 cells in 100ul.
* This antibody should be used at the same concentration as the test antibody.

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References for Mouse IgG2b Negative Control antibody

  1. Shoham, T. et al. (2001) Reduced expression of activin A in focal lymphoid agglomerates within nasal polyps.
    J Histochem Cytochem. 49 (10): 1245-52.
  2. Zheng, X. et al. (2002) Interleukin-3, but not granulocyte-macrophage colony-stimulating factor and interleukin-5, inhibits apoptosis of human basophils through phosphatidylinositol 3-kinase: requirement of NF-kappaB-dependent and -independent pathways.
    Immunology. 107 (3): 306-15.
  3. Grant, A.J. et al. (2002) Hepatic expression of secondary lymphoid chemokine (CCL21) promotes the development of portal-associated lymphoid tissue in chronic inflammatory liver disease.
    Am J Pathol. 160 (4): 1445-55.
  4. Dalli, J. et al. (2008) Annexin 1 mediates the rapid anti-inflammatory effects of neutrophil-derived microparticles.
    Blood. 112 (6): 2512-9.
  5. Kapetanovic, R. et al. (2012) Pig bone marrow-derived macrophages resemble human macrophages in their response to bacterial lipopolysaccharide.
    J Immunol. 188: 3382-94.
  6. Lawrence, J. et al. (2024) Porcine Monocyte DNA Traps Formed during Infection with Pathogenic Clostridioides difficile Strains
    Pathogens. 13 (3): 228.
  7. Maciag, S. et al. (2022) Effects of freezing storage on the stability of maternal cellular and humoral immune components in porcine colostrum.
    Vet Immunol Immunopathol. 254: 110520.
  8. Forner, R. et al. (2021) Distribution difference of colostrum-derived B and T cells subsets in gilts and sows.
    PLoS One. 16 (5): e0249366.
    9. View The Latest Product References

RRID
AB_324452

MCA691A488

1603 167702

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