Mouse IgG2b Negative Control antibody

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Mouse IgG2b Negative Control:Alexa Fluor® 488

Mouse IgG2b isotype control for measurement of non specific binding of mouse monoclonal antibodies. This antibody recognizes a rat surface antigen.

Mouse IgG2b Negative Control:Alexa Fluor® 647

Mouse IgG2b isotype control for measurement of non specific binding of mouse monoclonal antibodies. This antibody recognizes a rat surface antigen.

Mouse IgG2b Negative Control:APC

Mouse IgG2b isotype control for measurement of non specific binding of mouse monoclonal antibodies. This antibody recognizes a rat surface antigen.

Mouse IgG2b Negative Control:Preservative Free

Mouse IgG2b isotype control for measurement of non specific binding of mouse monoclonal antibodies. This antibody recognizes a rat surface antigen.

Mouse IgG2b Negative Control:Biotin

Mouse IgG2b isotype control for measurement of non specific binding of mouse monoclonal antibodies. This antibody recognizes a rat surface antigen.

Mouse IgG2b Negative Control:FITC

Mouse IgG2b isotype control for measurement of non specific binding of mouse monoclonal antibodies. This antibody recognizes a rat surface antigen.

Mouse IgG2b Negative Control:Pacific Blue®

Mouse IgG2b isotype control for measurement of non specific binding of mouse monoclonal antibodies. This antibody recognizes a rat surface antigen.

Mouse IgG2b Negative Control

Mouse IgG2b isotype control for measurement of non specific binding of mouse monoclonal antibodies. This antibody recognizes a rat surface antigen.

Mouse IgG2b Negative Control:RPE

Mouse IgG2b isotype control for measurement of non specific binding of mouse monoclonal antibodies. This antibody recognizes a rat surface antigen.

Mouse IgG2b Negative Control:RPE-Alexa Fluor® 647

Mouse IgG2b isotype control for measurement of non specific binding of mouse monoclonal antibodies. This antibody recognizes a rat surface antigen.

Mouse IgG2b Negative Control:RPE-Cy5

Mouse IgG2b isotype control for measurement of non specific binding of mouse monoclonal antibodies. This antibody recognizes a rat surface antigen.

Mouse IgG2b Negative Control:Alexa Fluor® 700

Mouse IgG2b isotype control for measurement of non specific binding of mouse monoclonal antibodies. This antibody recognizes a rat surface antigen.

Product Type
Negative/Isotype Control
Isotype
IgG2b
Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
MCA691A488 F 100 Tests/1ml
MCA691A647 F 100 Tests/1ml
MCA691APC F 100 Tests
MCA691XZ C F P R RE 1 mg
MCA691B F 100 Tests
MCA691F F 100 Tests
MCA691PB F 100 Tests/1ml
MCA691 F 100 Tests
MCA691PE F 100 Tests
MCA691P647 F 100 Tests/1ml
MCA691C F 100 Tests
MCA691A700 F 100 Tests/1ml
Mouse IgG2b Negative Control is negative on all human cells and cell lines tested. This antibody recognises a rat cell surface marker, and therefore cannot be used as a negative control in this species.

Test results show that MCA691C is also suitable for use as a negative control with bovine, ovine, porcine, canine and guinea-pig tissues.

Product Details

Target Species
Negative Control
Product Form
Purified IgG conjugated to Alexa Fluor® 488 - liquid
Product Form
Purified IgG conjugated to Alexa Fluor® 647 - liquid
Product Form
Purified IgG conjugated to Allophycocyanin (APC) - lyophilised
Product Form
Purified IgG - liquid
Product Form
Purified IgG conjugated to Biotin - liquid
Product Form
Purified IgG conjugated to Fluorescein Isothiocyanate Isomer 1 (FITC) - liquid
Product Form
Purified IgG conjugated to Pacific Blue® - liquid
Product Form
Purified IgG - liquid
Product Form
Purified IgG conjugated to R. Phycoerythrin (RPE) - lyophilized
Product Form
Purified IgG conjugated to R. Phycoerythrin (RPE) - Alexa Fluor® 647 - lyophilized
Product Form
Purified IgG conjugated to R Phycoerythrin (RPE) Cy5 - liquid
Product Form
Purified IgG conjugated to Alexa Fluor® 700 - liquid
Reconstitution
Reconstitute with 1.0ml distilled water
Care should be taken during reconstitution as the protein may appear as a film at the bottom of the vial. Bio-Rad recommend that the vial is gently mixed after reconstitution.
Reconstitution
Reconstitute with 1 ml distilled water
Reconstitution
Reconstitute with 1 ml distilled water
Preparation
Purified IgG prepared by affinity chromatography on Protein A from tissue culture supernatant
Preparation
Purified IgG prepared by affinity chromatography on Protein A from tissue culture supernatant
Preparation
Purified IgG prepared by ion exchange chromatography from tissue culture supernatant
Preparation
Purified IgG prepared by affinity chromatography on Protein A
Preparation
Purified IgG prepared by affinity chromatography on Protein A from tissue culture supernatant
Preparation
Purified IgG prepared by affinity chromatography on Protein A from tissue culture supernatant
Preparation
Purified IgG prepared by affinity chromatography on Protein A from tissue culture supernatant
Preparation
Purified IgG prepared by affinity chromatography on Protein A
Preparation
Purified IgG prepared by affinity chromatography on Protein A from tissue culture supernatant
Preparation
Purified IgG prepared by affinity chromatography on Protein A from tissue culture supernatant
Preparation
Purified IgG prepared by affinity chromatography on Protein A from tissue culture supernatant
Preparation
Purified IgG prepared by affinity chromatography on Protein A from tissue culture supernatant
Buffer Solution
Phosphate buffered saline
Buffer Solution
Phosphate buffered saline
Buffer Solution
Phosphate buffered saline
Buffer Solution
Phosphate buffered saline
Buffer Solution
Phosphate buffered saline
Buffer Solution
Phosphate buffered saline
Buffer Solution
Phosphate buffered saline
Buffer Solution
Phosphate buffered saline
Buffer Solution
Phosphate buffered saline
Buffer Solution
Phosphate buffered saline
Buffer Solution
Phosphate buffered saline.
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.09%Sodium Azide
1%Bovine Serum Albumin
Preservative Stabilisers
0.09%Sodium Azide
1%Bovine Serum Albumin
Preservative Stabilisers
0.09%Sodium Azide
1%Bovine Serum Albumin
Preservative Stabilisers
None present
Preservative Stabilisers
0.09%Sodium Azide
1%Bovine Serum Albumin
Preservative Stabilisers
0.09%Sodium Azide
1%Bovine Serum Albumin
Preservative Stabilisers
0.09%Sodium Azide
1%Bovine Serum Albumin
Preservative Stabilisers
0.09%Sodium Azide
1%Bovine Serum Albumin
Preservative Stabilisers
0.09%Sodium Azide
1%Bovine Serum Albumin
Preservative Stabilisers
0.09%Sodium Azide
1%Bovine Serum Albumin
Preservative Stabilisers
0.09%Sodium Azide
0.2%Bovine Serum Albumin
Preservative Stabilisers
0.09%Sodium Azide
1%Bovine Serum Albumin
Approx. Protein Concentrations
IgG concentration 0.05 mg/ml
Approx. Protein Concentrations
IgG concentration 0.05 mg/ml
Approx. Protein Concentrations
IgG concentration 1.0 mg/ml
Approx. Protein Concentrations
IgG concentration 0.1 mg/ml
Approx. Protein Concentrations
IgG concentration 0.1 mg/ml
Approx. Protein Concentrations
IgG concentration 0.05 mg/ml
Approx. Protein Concentrations
IgG concentration 0.1 mg/ml
Approx. Protein Concentrations
IgG concentration 0.05 mg/ml

Storage Information

Storage
Store at +4oC or at -20oC if preferred.

This product should be stored undiluted.

Storage in frost-free freezers is not recommended. This product is photosensitive and should be protected from light.

Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Storage
Store at +4oC or at -20oC if preferred.

This product should be stored undiluted.

Storage in frost-free freezers is not recommended. This product is photosensitive and should be protected from light.

Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Storage
Store at +4oC.

DO NOT FREEZE.

This product should be stored undiluted. This product is photosensitive and should be protected from light. Should this product contain a precipitate we recommend microcentrifugation before use.
Storage
Store at -20oC only.

This product should be stored undiluted.

Storage in frost-free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Storage
Store at +4oC or at -20oC if preferred.

This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Storage
Store at +4oC or at -20oC if preferred.

This product should be stored undiluted.

Storage in frost-free freezers is not recommended. This product is photosensitive and should be protected from light.

Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Storage
Store at +4oC or at -20oC if preferred.

This product should be stored undiluted.

Storage in frost-free freezers is not recommended. This product is photosensitive and should be protected from light.

Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Storage
Store at +4oC for one month or at -20oC for longer.

This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Storage
Prior to reconstitution store at +4oC. Following reconstitution store at +4oC.

This product should be stored undiluted.

DO NOT FREEZE. This product is photosensitive and should be protected from light. Should this product contain a precipitate we recommend microcentrifugation before use.
Storage
Prior to reconstitution store at +4oC. Following reconstitution store at +4oC.

This product should be stored undiluted.

DO NOT FREEZE. This product is photosensitive and should be protected from light. Should this product contain a precipitate we recommend microcentrifugation before use.
Storage
Store at +4oC.

DO NOT FREEZE.

This product should be stored undiluted. This product is photosensitive and should be protected from light. Should this product contain a precipitate we recommend microcentrifugation before use.
Storage
Store at +4oC or at -20oC if preferred.

This product should be stored undiluted.

Storage in frost-free freezers is not recommended. This product is photosensitive and should be protected from light.

Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Shelf Life
18 months from date of despatch.
Shelf Life
18 months from date of despatch.
Shelf Life
6 months from date of despatch.
Shelf Life
18 months from date of despatch.
Shelf Life
18 months from date of despatch.
Shelf Life
18 months from date of despatch.
Shelf Life
18 months from date of despatch.
Shelf Life
18 months from date of despatch.
Shelf Life
12 months from date of reconstitution.
Shelf Life
12 months from date of reconstitution.
Shelf Life
Please see label for expiry date.
Shelf Life
18 months from date of despatch.

More Information

Acknowledgements
This product is provided under an intellectual property licence from Life Technologies Corporation. The transfer of this product is contingent on the buyer using the purchase product solely in research, excluding contract research or any fee for service research, and the buyer must not sell or otherwise transfer this product or its components for (a) diagnostic, therapeutic or prophylactic purposes; (b) testing, analysis or screening services, or information in return for compensation on a per-test basis; (c) manufacturing or quality assurance or quality control, or (d) resale, whether or not resold for use in research. For information on purchasing a license to this product for purposes other than as described above, contact Life Technologies Corporation, 5791 Van Allen Way, Carlsbad CA 92008 USA or outlicensing@thermofisher.com
Acknowledgements
This product is provided under an intellectual property licence from Life Technologies Corporation. The transfer of this product is contingent on the buyer using the purchase product solely in research, excluding contract research or any fee for service research, and the buyer must not sell or otherwise transfer this product or its components for (a) diagnostic, therapeutic or prophylactic purposes; (b) testing, analysis or screening services, or information in return for compensation on a per-test basis; (c) manufacturing or quality assurance or quality control, or (d) resale, whether or not resold for use in research. For information on purchasing a license to this product for purposes other than as described above, contact Life Technologies Corporation, 5791 Van Allen Way, Carlsbad CA 92008 USA or outlicensing@thermofisher.com
Acknowledgements
The Pacific Blue® dye antibody conjugates in this product are sold under license from Molecular Probes, Inc. for research use only, except for use in combination with microarrays, and are covered by pending and issued patents.
Acknowledgements
This product is provided under an intellectual property license from Life Technologies Corporation. The transfer of this product is contingent on the buyer using the purchased product solely in research conducted by the buyer, excluding contract research or any fee for service research, and the buyer must not sell or otherwise transfer this product or its components for (a) diagnostic, therapeutic or prophylactic purposes; (b) testing, analysis or screening services, or information in return for compensation on a per-test basis; (c) manufacturing or quality assurance or quality control, or (d) resale, whether or not resold for use in research. For information on purchasing a license to this product for purposes other than as described above, contact Life Technologies Corporation, 5791 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@thermofisher.com
Acknowledgements
Cy® and CyDye® are registered trademarks of GE Healthcare
Acknowledgements
This product is provided under an intellectual property licence from Life Technologies Corporation. The transfer of this product is contingent on the buyer using the purchase product solely in research, excluding contract research or any fee for service research, and the buyer must not sell or otherwise transfer this product or its components for (a) diagnostic, therapeutic or prophylactic purposes; (b) testing, analysis or screening services, or information in return for compensation on a per-test basis; (c) manufacturing or quality assurance or quality control, or (d) resale, whether or not resold for use in research. For information on purchasing a license to this product for purposes other than as described above, contact Life Technologies Corporation, 5791 Van Allen Way, Carlsbad CA 92008 USA or outlicensing@thermofisher.com
Regulatory
For research purposes only

Applications of Mouse IgG2b Negative Control antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Flow Cytometry *
Flow Cytometry *
Flow Cytometry *
Flow Cytometry *
Immunohistology - Frozen *
Immunohistology - Paraffin *
Immunohistology - Resin *
Radioimmunoassays *
Flow Cytometry *
Flow Cytometry Neat 1/10
Flow Cytometry *
Flow Cytometry *
Flow Cytometry *
Flow Cytometry *
Flow Cytometry *
Flow Cytometry *
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only.
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only.
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. *This antibody should be used at the same concentration as the test antibody.
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only.
*It is recommended that the user adjusts the concentration of this reagent to be the same as the test reagents.
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only.
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only.
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only.
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only.
*It is recommended that the user titrates the antibody for use in their system to a concentration equivalent to that of their test reagents.
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only.
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only.
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. *It is recommended that the user dilutes the antibody to a concentration equivalent to their test reagent.
Flow Cytometry
Use 10ul of the suggested working dilution to label 106 cells in 100ul.
* This antibody should be used at the same concentration as the test antibody.
Flow Cytometry
Use 10ul of the suggested working dilution to label 106 cells in 100ul.
* It is suggested that the user adjusts the concentration of this reagent to be the same as the test reagents.
Flow Cytometry
Use 10ul of the suggested working dilution to label 106 cells or cells or 100ul whole blood.
Flow Cytometry
Use 10ul of the suggested working dilution to label 1x106 cells in 100ul.
Flow Cytometry
Use 10ul of the suggested working dilution to label 106 cells in 100ul.
*It is suggested that the user adjusts the concentration of this reagent to be the same as the test reagent.
Flow Cytometry
Use 10ul of the suggested working dilution to label 106 cells in 100ul.
* It is suggested that the user adjusts the concentration of this reagent to be the same as the test reagents.
Flow Cytometry
Use 10ul of the suggested working dilution to label 106 cells in 100ul.
* This antibody should be used at the same concentration as the test antibody.
Flow Cytometry
Use 10ul of the suggested working dilution to label 1x106 cells in 100ul.
Flow Cytometry
Use 10ul of the suggested working dilution to label 106 cells or 100ul whole blood
* It is recommended that the user dilutes the antibody for use in their own system to a concentration equivalent to their test antibody.
Flow Cytometry
Use 10ul of the suggested working dilution to label 106 cells or 100ul whole blood
* It is recommended that the user adjusts the concentration of this reagent to the same as the test reagents.
Flow Cytometry
Use 5ul of the suggested working dilution to label 106 cells or 100ul whole peripheral blood.
Flow Cytometry
Use 10ul of the suggested working dilution to label 106 cells in 100ul.
* It is suggested that the user adjusts the concentration of this reagent to be the same as the test reagents.

Product Specific References

References for Mouse IgG2b Negative Control antibody

  1. Grant, A.J. et al. (2002) Hepatic expression of secondary lymphoid chemokine (CCL21) promotes the development of portal-associated lymphoid tissue in chronic inflammatory liver disease.
    Am J Pathol. 160 (4): 1445-55.
  2. Zheng, X. et al. (2002) Interleukin-3, but not granulocyte-macrophage colony-stimulating factor and interleukin-5, inhibits apoptosis of human basophils through phosphatidylinositol 3-kinase: requirement of NF-KappaB-dependent and-independent pathways.
    Immunology. 107:306-315.
  3. Dalli, J. et al. (2008) Annexin 1 mediates the rapid anti-inflammatory effects of neutrophil-derived microparticles.
    Blood. 112 (6): 2512-9.
  4. Kapetanovic, R. et al. (2012) Pig bone marrow-derived macrophages resemble human macrophages in their response to bacterial lipopolysaccharide.
    J Immunol. 188: 3382-94.
  5. Shoham, T. et al. (2001) Reduced expression of activin A in focal lymphoid agglomerates within nasal polyps.
    J Histochem Cytochem. 49 (10): 1245-52.