CD4 Antibody | OX-38

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CD4 Antibody | OX-38 gallery image 1

Staining of rat splenocytes with Mouse anti Rat CD4 domain 1: Azide Free (MCA372XZ)

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CD4 Antibody | OX-38 gallery image 2

Immunoperoxidase staining of rat lymph node cryosection with Mouse anti Rat CD4 antibody, clone OX-38 (MCA372) followed by horseradish peroxidase conjugated Goat anti Mouse IgG2a (STAR133P) as a detection reagent. Low power

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CD4 Antibody | OX-38 gallery image 3

Immunoperoxidase staining of rat lymph node cryosection with Mouse anti Rat CD4 antibody, clone OX-38 (MCA372) followed by horseradish peroxidase conjugated Goat anti Mouse IgG2a (STAR133P) as a detection reagent. Medium power

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CD4 Antibody | OX-38 gallery image 4

Immunoperoxidase staining of rat lymph node cryosection with Mouse anti Rat CD4 antibody, clone OX-38 (MCA372) followed by horseradish peroxidase conjugated Goat anti Mouse IgG2a (STAR133P) as a detection reagent. High power

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CD4 Antibody | OX-38 gallery image 5

Immunofluorescence staining of rat lymph node cryosection with Mouse anti Rat CD4, clone OX-38 (MCA372). Nuclei are counterstained blue with DAPI

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CD4 Antibody | OX-38 gallery image 6

Published customer image:
Mouse anti Rat CD4 (Domain 1) antibody, clone OX-38 used for the identification of CD4 expressing cells in rat spleen by flow cytometry and immunofluorescence.
Image caption:
Assessment of proliferating lymphocytes in the spleen activated by CD28SA. a FCM analysis of the splenic lymphocytes for lymphocyte markers and EdU at day 3 after CD28SA injection (0.5 mg/rat). Note increase in TCRαβ+, CD4+, or CD25+ EdU+ cells but not CD8β+EdU+ cells compared to control. b Dose response of splenic lymphocytes to CD28SA. Absolute number of EdU+ proliferating cells with different lymphocyte markers/spleen, showing 1.0 mg group induces the highest response. c Triple immunofluorescent staining for lymphocyte markers (indirect staining with Alexa-680-conjugated anti-mouse IgG, green), EdU (Alexa-594-conjugated azide, red) and type IV collagen (indirect staining with AMCA-conjugated anti-rabbit IgG, white). Day 3 after CD28SA injection (0.5 mg/rat). Pseudocolors were assigned using AxioVision software. Scale bar 100 μm. The splenic PALS area with the central arteries is depicted by type IV collagen. Proliferating cells with different markers are shown as green cells with red nuclei (inset of lower panel).

From: Kitazawa Y, Ueta H, Hünig T, Sawanobori Y, Matsuno K. A novel multicolor immunostaining method using ethynyl deoxyuridine for analysis of in situ immunoproliferative response.
Histochem Cell Biol. 2015 Sep;144(3):195-208.

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CD4 Antibody | OX-38 gallery image 7

Published customer image:
Mouse anti Rat CD4 (Domain 1) antibody, clone OX-38 used for the identification of CD4 expressing cells in rat spleen by flow cytometry.
Image caption:
FCM for proliferating Tregs induced by CD28SA. a Three-color FCM analysis of the splenic lymphocytes for lymphocyte markers, Treg transcription factor (Foxp3), and EdU at day 3 after CD28SA injection (0.5 mg/rat). b Dose response of Foxp3+Tregs with different markers to CD28SA. Absolute number of EdU+ proliferating Tregs with different lymphocyte markers/spleen, showing 0.5–1.0 mg induces an intense response

From: Kitazawa Y, Ueta H, Hünig T, Sawanobori Y, Matsuno K. A novel multicolor immunostaining method using ethynyl deoxyuridine for analysis of in situ immunoproliferative response.
Histochem Cell Biol. 2015 Sep;144(3):195-208.

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  • Mouse anti Rat CD4 (Domain 1):Preservative Free
  • Mouse anti Rat CD4 (Domain 1)
(Rated 0.0 out of 5 based on 0 customer reviews)
  • Product Type
    Monoclonal Antibody
  • Clone
    OX-38
  • Isotype
    IgG2a
2 Formats Available
    Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
    MCA372GC, F, IPdatasheet pdfdatasheet pdf0.25 mg
    MCA372G
    MCA372XZC, F, IPdatasheet pdfdatasheet pdf1 mg
    MCA372XZ
    Summary
    Secondary Antibodies
    Negative Isotype Controls
    Useful Reagents
    Positive Controls
    Histology Controls
    More Images
    References
    Reviews
    -
    • Mouse anti Rat CD4 (Domain 1) antibody, clone OX-38 recognizes the rat CD4 cell surface antigen, also known as W3/25 antigen or T-cell surface antigen T4/Leu-3. CD4 is a 430 amino acid ~55 kDa single pass type I transmembrane glycoprotein expressed by T helper cells, monocytes and macrophages.

      Clone OX-38 competes for binding with clone W3/25.

      Mouse anti Rat CD4 (Domain 1) antibody, clone OX-38 has been utilized successfully for the in vitro depletion of T helper cells from slice cultures in studies looking at myelin loss in multiple sclerosis, the observed disruption being abrogated in these experimental conditions by T-cell depletion (Pusic et al. 2015).
    • Intended Use
    • Target Species
      Rat
    • Product Form
      Purified IgG - liquid
      Purified IgG - liquid
    • Reconstitution
    • Preparation
      Purified IgG prepared by affinity chromatography on Protein G
      Purified IgG prepared by affinity chromatography on Protein G
    • Preservative Stabilisers
      None Present
      0.09%Sodium Azide
    • Immunogen
      MLR generated rat T cells.
    • Purity
    • Approx. Protein Concentrations
      IgG concentration 1.0 mg/ml
      IgG concentration 1.0 mg/ml
    • Reagents In The Kit
    • Preparing The Antibody
    • Test Principle
    • Buffer Solution
      Phosphate buffered saline
      Phosphate buffered saline
    • Fusion Partners
      Spleen cells from immunised mice were fused with cells of the mouse NS1 myeloma cell line.
    • Storage
      Store at -20oC only.

      This product should be stored undiluted.

      Storage in frost-free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
      Store at +4oC or at -20oC if preferred.

      This product should be stored undiluted.
      Storage in frost-free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
    • Shelf Life
      18 months from date of despatch.
      18 months from date of despatch.
    • GO Terms
    • UniProt
    • Entrez Gene
    • Acknowledgements
    • Regulatory
      For research purposes only
    • This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.

    • Application NameYesNoMin DilutionMax Dilution
      Flow Cytometry1/1001/200
      Immunohistology - Frozen
      Immunoprecipitation
    • Application NameYesNoMin DilutionMax Dilution
      Flow Cytometry1/1001/200
      Immunohistology - Frozen
      Immunoprecipitation

    • Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
    • Technical Advice
    • Technical Advice
    • Recommended Protocol
    • Recommended Protocol
    • ELISA
    • ELISA
    • Immunohistology
    • Immunohistology
    • Histology Positive Control Tissue
    • Histology Positive Control Tissue
    • Immunofluorescence
    • Immunofluorescence
    • Western Blotting
    • Western Blotting
    • Instructions For Use
    • Instructions For Use

    Additional CD4 Antibody Formats

    Formats Clone Applications Sizes available
    CD4 Antibody : Purified OX-38 C, F, IP 0.25 mg
    CD4 Antibody : Preservative Free OX-38 C, F, IP 1 mg
    • Copyright © 2016 Bio-Rad

    Recommended Secondary Antibody

      DescriptionProduct CodePack SizeApplicationsList PriceQuantity
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      Rabbit F(ab')2 anti Mouse IgG:FITCSTAR9B1 mgF
      STAR9B
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      Goat anti Mouse IgG (H/L):HRP (Multi Species Adsorbed)STAR117P0.5 mgE, WB
      STAR117P
      Goat anti Mouse IgG:HRP (Rat Adsorbed)STAR770.5 mgC, E, P
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      Rabbit F(ab')2 anti Mouse IgG:RPESTAR12A1 mlF
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      Goat anti Mouse IgG:RPE (Rat Adsorbed)STAR761 mlF
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      DescriptionProduct CodePack SizeApplicationsList PriceQuantity
      Goat anti Mouse IgG (H/L):Alk. Phos. (Multi Species Adsorbed)STAR117A0.5 mgE, WB
      STAR117A
      Goat anti Mouse IgG/A/M:Alk. Phos.STAR87A1 mgC, E, WB
      STAR87A
      Goat anti Mouse IgG (H/L):DyLight®488 (Multi Species Adsorbed)STAR117D488GA0.1 mgF, IF
      STAR117D488GA
      Goat anti Mouse IgG (H/L):DyLight®549 (Multi Species Adsorbed)STAR117D549GA0.1 mgF, IF
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      Goat anti Mouse IgG (H/L):DyLight®649 (Multi Species Adsorbed)STAR117D649GA0.1 mgF, IF
      STAR117D649GA
      Goat anti Mouse IgG (H/L):DyLight®680 (Multi Species Adsorbed)STAR117D680GA0.1 mgF, WB
      STAR117D680GA
      Goat anti Mouse IgG (H/L):DyLight®800 (Multi Species Adsorbed)STAR117D800GA0.1 mgF, IF, WB
      STAR117D800GA
      Rabbit F(ab')2 anti Mouse IgG:Dylight®800STAR8D800GA0.1 mgF, IF, WB
      STAR8D800GA
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      Recommended Negative Isotype Control

        DescriptionProduct CodePack SizeApplicationsList PriceQuantity
        Mouse IgG2a Negative Control:Preservative FreeMCA1210XZ1 mgF
        MCA1210XZ
        DescriptionProduct CodePack SizeApplicationsList PriceQuantity
        Mouse IgG2a Negative ControlMCA1210100 TestsF
        MCA1210

        Useful Reagents

          Recommended Positive Controls

            Histology Controls

              References

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