IgG2a antibody
HRP conjugated Goat anti Mouse IgG2a antibody (STAR133P) used to detect bound anti IAV antibodies in airway organoids by immunohistochemistryon formalin fixed, paraffin embedded sections.
Image caption:
Influenza A virus infection and cytokine profiling of the apical compartment of epithelial cell mono-cultures or in co-culture with endothelial cells.
(C) Detection of influenza A virus (IAV) nucleoprotein (NP) by immunohistochemistry (IHC) of the AO at ALI-LMEC co-cultures 24 h post-inoculation. (D) Hematoxylin and eosin (H&E) staining of the co-cultures 72 h post-inoculation (scale bar 20 μm).
From: Bauer L, Rijsbergen LC, Leijten L, Benavides FF, Noack D, Lamers MM, Haagmans BL, de Vries RD, de Swart RL, van Riel D.
The pro-inflammatory response to influenza A virus infection is fueled by endothelial cells.
Life Sci Alliance. 2023 Apr 18;6(7):e202201837.
doi: 10.26508/lsa.202201837.
This image is from an open access article distributed under terms of a Creative Commons Attribution License.
Goat anti Mouse IgG2a:HRP
- Product Type
- Polyclonal Antibody
- Isotype
- Polyclonal IgG
- Specificity
- IgG2a
Antibody Quality and Performance Guarantee
| Goat anti Mouse IgG2a antibody recognizes Mouse IgG2a. This antibody has been cross absorbed against mouse IgM, IgG1, IgG2b, IgG3 and IgA, pooled human sera and purified human paraproteins. Goat anti Mouse IgG2a antibody shows minimal cross-reactivity with human immunoglobulins. |
- Target Species
- Mouse
- Species Cross-Reactivity
-
Target Species Cross Reactivity Human - N.B. Antibody reactivity and working conditions may vary between species.
- Product Form
- Purified IgG conjugated to Horseradish Peroxidase (HRP) - liquid
- Antiserum Preparation
- Antisera to mouse IgG2a were raised by repeated immunisation of goats with purified antigen. Purified IgG was prepared from whole serum by affinity chromatography.
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
- 0.01% Thiomersal
50% Glycerol - Immunogen
- IgG2a paraproteins from BALB/c mice
- Regulatory
- For research purposes only
- Guarantee
- 12 months from date of despatch
This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
| Application Name | Verified | Min Dilution | Max Dilution |
|---|---|---|---|
| ELISA |  |
1/4000 | 1/8000 |
| Immunohistology - Frozen | |
||
| Immunohistology - Paraffin | |
||
| Western Blotting | |
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
| Description | Product Code | Applications | Pack Size | List Price | Your Price | Quantity | |
|---|---|---|---|---|---|---|---|
| AbGUARD® HRP Stabilizer Plus | BUF052A | C E P WB | 100 ml | Log in | |||
| List Price | Your Price | ||||||
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| Description | AbGUARD® HRP Stabilizer Plus | ||||||
| AbGUARD® HRP Stabilizer Plus | BUF052B | C E P WB | 500 ml | Log in | |||
| List Price | Your Price | ||||||
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| Description | AbGUARD® HRP Stabilizer Plus | ||||||
| AbGUARD® HRP Stabilizer Plus | BUF052C | C E P WB | 1000 ml | Log in | |||
| List Price | Your Price | ||||||
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| Description | AbGUARD® HRP Stabilizer Plus | ||||||
| TMB Signal+ | BUF054A | E | 100 ml | Log in | |||
| List Price | Your Price | ||||||
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| Description | TMB Signal+ | ||||||
| TMB Core | BUF056A | E | 100 ml | Log in | |||
| List Price | Your Price | ||||||
| Log in | |||||||
| Description | TMB Core | ||||||
| TMB Core+ | BUF062A | E | 100 ml | Log in | |||
| List Price | Your Price | ||||||
| Log in | |||||||
| Description | TMB Core+ | ||||||
References for IgG2a antibody
-
Knipping, K. et al. (2011) A gastrointestinal rotavirus infection mouse model for immune modulation studies.
Virol J. 8: 109. -
Bagai, U. & Pawar, A. (2013) A blood stage fraction of Plasmodium berghei. induces protective and long lasting immune response in BALB/c mice.
Parasitol Int. 62 (3): 329-36. -
Hwang, S.R. et al. (2015) Altered expression levels of neurodevelopmental proteins in fetal brains of BTBR T+tf/J mice with autism-like behavioral characteristics.
J Toxicol Environ Health A. 78 (8): 516-23. -
Zhao, Z. et al. (2015) Multiple B-cell epitope vaccine induces a Staphylococcus enterotoxin B-specific IgG1 protective response against MRSA infection.
Sci Rep. 5: 12371. -
Minaei, S. et al. (2018) Propranolol efficacy as a novel adjuvant for immunization against Toxoplasma gondii. tachyzoites.
Exp Parasitol. 194: 60-66. -
Kushwaha, V. et al. (2019) Troponin 1 of human filarial parasite Brugia malayi.: cDNA cloning, expression, purification, and its immunoprophylactic potential.
Parasitol Res. 118 (6): 1849-63. -
Nedumpun, T. et al. (2019) Negative Immunomodulatory Effects of Type 2 Porcine Reproductive and Respiratory Syndrome Virus-Induced Interleukin-1 Receptor Antagonist on Porcine Innate and Adaptive Immune Functions.
Front Immunol. 10: 579. -
Gatkowska, J. et al. (2019) The Impact of the Antigenic Composition of Chimeric Proteins on Their Immunoprotective Activity against Chronic Toxoplasmosis in Mice.
Vaccines (Basel). 7(4):154.
View The Latest Product References
-
Mola, S. et al. (2020) A transcriptome-based approach to identify functional modules within and across primary human immune cells.
PLoS One. 15 (5): e0233543. -
Han, H. et al. (2021) Metal arsenic mediated enhancement of type-2 immunity in brains with altered locomotive activities in mice with autism-like behavioral characteristics
Toxicological Research. 38 (1): 27-33. -
Sessevmez, M. et al. (2023) Induction of humoral and cell-mediated immunity in mice by chitosan-curdlan composite nanoparticles administered intranasally and subcutaneously
J Drug Deliv Sci Technol. 86: 104704. -
Bauer, L. et al. (2023) The pro-inflammatory response to influenza A virus infection is fueled by endothelial cells.
Life Sci Alliance. 6 (7): e202201837. -
Faber, E. et al. (2024) Identification of T cell and linear B cell epitopes on African horse sickness virus serotype 4 proteins VP1-1, VP2, VP4, VP7 and NS3.
Vaccine. 42 (2): 136-45. -
Kusma, S. et al. (2024) Oral subacute polypropylene microplastics administration effect on potential immunotoxicity in ICR mice.
J Toxicol Environ Health A. : 1-10 [Epub ahead of print]. -
Wu, Y. et al. (2020) Sterilizing immunity against SARS-CoV-2 in hamsters conferred by a novel recombinant subunit vaccine
bioRχiv: 20 Dec. [Epub ahead of print].
- RRID
- AB_1102655
- UniProt
- P01865
- P01863
- P01864
- Entrez Gene
- Igh-1a
- Igh-1a
- Igh-1a
- GO Terms
- GO:0003823 antigen binding
- GO:0002455 humoral immune response mediated by circulating immunoglobulin
- GO:0019731 antibacterial humoral response
- GO:0042571 immunoglobulin complex, circulating
- GO:0005886 plasma membrane
- GO:0016021 integral to membrane
STAR133P
149975
155597
156797
162640
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