Atezolizumab antibody | AbD40640
A microtiter plate was coated overnight with various antigens at a concentration of 5 μg/ml. After washing and blocking with PBST + 5% BSA, the atezolizumab/PD-L1 complex was formed by adding atezolizumab at the concentration of 5 μg/ml to wells coated with PD-L1. Detection was performed using HRP-conjugated Human Anti-Atezolizumab (Drug/Target) Antibody, clone AbD40640pap (TZA013P) at a concentration of 0.2 μg/ml in HISPEC Assay Diluent (BUF049A) followed by QuantaBlu Fluorogenic Peroxidase Substrate.
A microtiter plate was coated overnight with PD-L1, atezolizumab, human IgG1 kappa or trastuzumab at a concentration of 5 µg/ml. After washing and blocking with PBST + 5% BSA, the atezolizumab/PD-L1 complex was formed by adding 5 µg/ml atezolizumab to the wells coated with PD-L1. Detection was performed using HRP-conjugated Human Anti-Atezolizumab (Drug/Target) Antibody, clone AbD40640pap (TZA013P) titrated to the given concentrations in HISPEC Assay Diluent (BUF049A) followed by QuantaBlu Fluorogenic Peroxidase Substrate. Data are shown as the mean of three measurements.
A microtiter plate was coated overnight with human PD-L1 at a concentration of 5 µg/ml. After washing and blocking with PBST + 5% BSA, 10% human serum was added spiked with increasing concentrations of atezolizumab. Detection was performed using HRP-conjugated Human anti-Atezolizumab (Drug/Target) Antibody, clone AbD40640pap (TZA013P) at a concentration of 0.5 mg/ml in HISPEC Assay Diluent (BUF049A) followed by QuantaBlu Fluorogenic Peroxidase Substrate. Data are shown as the mean of three measurements.
Human anti Atezolizumab (Drug/Target Complex)
- Product Type
- Monoclonal Antibody
- Clone
- AbD40640
- Isotype
- Fab antibody
- Specificity
- Atezolizumab
- Region
- DRUG/TARGET COMPLEX
Antibody Quality and Performance Guarantee
TZA013
| Human Anti-Atezolizumab (Drug/Target Complex) Antibody, clone AbD40640 specifically recognizes the antibody drug atezolizumab when in complex with its target PD-L1 (programmed cell death ligand 1). It does not recognize free atezolizumab nor unbound PD-L1. It can be used to measure levels of atezolizumab in patient samples. This TrailBlazer Antibody is available in a purified monovalent Fab format tagged with a SpyTag2 at the C terminus of the Fab antibody heavy chain (TZA013), enabling the user to couple it to a SpyCatcher reagent for conversion to alternative formats in less than one hour. It is also available as a bivalent Fab format pre-coupled to the an HRP-conjugated BiSpyCatcher2 for direct detection of atezolizumab under product code TZA013P. View a summary of all SpyCatcher products Either format can be used to develop a pharmacokinetic (PK) antigen capture assay to measure free atezolizumab captured on immobilized PD-L1. Atezolizumab (brand name Tecentriq®) is a human monoclonal IgG1 antibody that binds directly to Programmed Death Ligand 1 (PD-L1, CD274) and provides a dual blockade of both the PD-1 and B7.1 receptors on T-cells and antigen presenting cells. It is one of the named “checkpoint inhibitor” drugs. PD-L1/PD-1 mediated inhibition of the immune response is thereby released, including reactivation of the anti-tumour immune response without induction of antibody-dependent cellular cytotoxicity. The drug is used to treat urothelial carcinoma, non-small cell lung cancer, triple-negative breast cancer, small cell lung cancer and hepatocellular carcinoma. It is used either on its own or in combination for cancers which are advanced or metastatic. |
|
- Product Form
- A monovalent human recombinant Fab (lambda light chain) selected from the HuCAL® phage display library, expressed in a proprietary E. coli strain. The antibody is tagged with a DYKDDDDK tag, a SpyTag version 2 (VPTIVMVDAYKRYK) and a His6-tag (HHHHHH) at the C-terminus of the antibody heavy chain. This antibody is supplied as a liquid.
- Preparation
- Metal chelate affinity chromatography
- Source
- E.coli
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
- 0.0095% MIT
- Immunogen
- Atezolizumab/PD-L1 complex
- Affinity
- The monovalent intrinsic affinity of AbD40640ad was measured as KD = 91 nM by real time, label free molecular interaction analysis on immobilized PD-L1/atezolizumab complex.
- Approx. Protein Concentrations
- Antibody concentration 0.5 mg/ml
- Regulatory
- For research purposes only
- Guarantee
- 12 months from date of despatch
- Acknowledgements
- This product and/or its use is covered by claims of U.S. patents, and/or pending U.S. and non-U.S. patent applications owned by or under license to Bio-Rad Laboratories, Inc. See bio-rad.com/en-us/trademarks for details.
His-tag is a registered trademark of EMD Biosciences.
Tecentriq is a trademark of Roche/ Genentech. - Licensed Use
- For in vitro research purposes and for commercial applications for the provision of in vitro testing services to support preclinical and clinical drug development. Any re-sale in any form or any other commercial application needs a written agreement with Bio-Rad.
This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
| Application Name | Verified | Min Dilution | Max Dilution |
|---|---|---|---|
| ELISA |  |
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
- Technical Advice
- Recommended protocols for coupling a Fab antibody with a SpyTag to a SpyCatcher can be found at SpyTag and SpyCatcher Products, and further information about HuCAL recombinant antibody technology can be found in the HuCAL Antibodies Technical Manual
- ELISA
- Clone AbD40640ad may be used in an indirect ELISA to detect the atezolizumab/PD-L1 complex or to detect atezolizumab bound to immobilized PD-L1.
Protocol: PK Antigen Capture ELISA
| Description | Product Code | Applications | Pack Size | List Price | Your Price | Quantity | |
|---|---|---|---|---|---|---|---|
| SpyTag3 Peptide | BLP086 | IY | 1 mg |
|
Log in | ||
| List Price | Your Price | ||||||
|
|
Log in | ||||||
| Description | SpyTag3 Peptide | ||||||
| Hispec Assay Diluent | BUF049A | E IY | 50 ml | Log in | |||
| List Price | Your Price | ||||||
| Log in | |||||||
| Description | Hispec Assay Diluent | ||||||
| Human anti Atezolizumab | HCA380 | E | 0.1 mg |
|
Log in | ||
| List Price | Your Price | ||||||
|
|
Log in | ||||||
| Description | Human anti Atezolizumab | ||||||
| Human anti Atezolizumab | HCA381 | E | 0.1 mg |
|
Log in | ||
| List Price | Your Price | ||||||
|
|
Log in | ||||||
| Description | Human anti Atezolizumab | ||||||
| Human anti Atezolizumab | HCA382 | E | 0.1 mg |
|
Log in | ||
| List Price | Your Price | ||||||
|
|
Log in | ||||||
| Description | Human anti Atezolizumab | ||||||
| Human anti Atezolizumab | TZA011 | E | 0.1 mg | Log in | |||
| List Price | Your Price | ||||||
| Log in | |||||||
| Description | Human anti Atezolizumab | ||||||
| Human anti Atezolizumab (Drug/Target Complex):HRP | TZA013P | E | 0.1 mg | Log in | |||
| List Price | Your Price | ||||||
| Log in | |||||||
| Description | Human anti Atezolizumab (Drug/Target Complex):HRP | ||||||
| BiSpyCatcher2:Biotin | TZC002B | IY | 1 mg | Log in | |||
| List Price | Your Price | ||||||
| Log in | |||||||
| Description | BiSpyCatcher2:Biotin | ||||||
| BiSpyCatcher2:HRP | TZC002P | IY | 1 mg | Log in | |||
| List Price | Your Price | ||||||
| Log in | |||||||
| Description | BiSpyCatcher2:HRP | ||||||
- Synonyms
- Tecentriq
TZA013
166513
If you cannot find the batch/lot you are looking for please contact our technical support team for assistance.
View more products with Atezolizumab specificity
Please Note: All Products are "FOR RESEARCH PURPOSES ONLY"
Always be the first to know.
When we launch new products and resources to help you achieve more in the lab.
Yes, sign me up