CD25 Antibody | K231.3B2

//www.bio-rad-antibodies.com/pig-porcine-cd25-antibody-k231-3b2-mca1736ga.html
CD25 Antibody | K231.3B2 gallery image 1

Porcine peripheral blood lymphocytes stained with Mouse anti Pig CD25 antibody, clone K231.3B2 (MCA1736GA) followed by FITC conjugated Goat anti Mouse IgG antibody (STAR117F), dual stained with RPE conjugated Mouse anti Pig CD4a antibody, clone MIL17 (MCA1749PE). Numbers indicate the percent cells in each quadrant

Enlarge
CD25 Antibody | K231.3B2 gallery image 2

Published customer image:
Mouse anti Pig CD25, clone K231.3B2 used for the evaluation of CD25 expression on lymphocytes in culture by flow cytometry.
Image caption:
Induction of Tregs from lymphocytes co-cultured with PRRSV-infected MoDCs. (A) Representative flow cytometry profile of lymphocytes following 3 days co-culture of PBMCs with PRRSV-infected MoDCs (upper layer), and only culture with PRRSV-infected PBMCs (lower layer). (B) Percentage of Foxp3+ cells in the gated CD4+CD25+ subpopulations of PBMCs co-cultured with PRRSV-infected MoDC. (C) Percentage of Foxp3+ cells in the gated CD4+ CD25+ subpopulations of PRRSV-infected PBMCs alone. (D) Sorted CD4+CD25high cells. (E) Percentage suppression at the indicated Tregs: peripheral lymphocyte ratios. The lymphocytes exposed to Marc-145 lysate-treated MoDCs were used as negative control. The percentage of suppression was calculated as follows: % suppression = 100 × [1 - (% proliferation w/PRRSV/ % proliferation w/mock)] [35]. Data from three independent experiments. All data analysis was done using one-way ANOVA and significant differences are shown (*P<0.05 and **P< 0.01).

From: Fan B, Liu X, Bai J, Li Y, Zhang Q, Jiang P (2015) The 15N and 46R Residues of Highly Pathogenic Porcine Reproductive and Respiratory Syndrome Virus Nucleocapsid Protein Enhance Regulatory T Lymphocytes Proliferation.
PLoS ONE 10(9): e0138772.

Enlarge
CD25 Antibody | K231.3B2 gallery image 3

Figure A. FITC conjugated mouse anti porcine CD4a (MCA1749F) and purified mouse IgG1 isotype control (MCA928) detected with goat anti mouse IgG1PE (STAR132PE). Figure B. FITC conjugated mouse anti porcine CD4a (MCA1749F) and purified mouse anti porcine CD25 (MCA1736GA) detected with goat anti mouse IgG1PE (STAR132PE). All experiments performed on red cell lysed porcine blood gated on mononuclear cells.

Enlarge
CD25 Antibody | K231.3B2 gallery image 4

Figure A. Purified mouse anti porcine CD25 (MCA1736GA) detected with goat anti mouse IgG1 PE (STAR132PE) and mouse IgG2b FITC isotype control (MCA691F). Figure B. Purified mouse anti porcine CD25 (MCA1736GA) detected with goat anti mouse IgG1 PE (STAR132PE) and mouse anti porcine CD4a FITC (MCA1749F). All experiments performed on red cell lysed porcine blood gated on mononuclear cells.

Enlarge
  • CD25 Antibody | K231.3B2 thumbnail image 1
  • CD25 Antibody | K231.3B2 thumbnail image 2
  • CD25 Antibody | K231.3B2 thumbnail image 3
  • CD25 Antibody | K231.3B2 thumbnail image 4
  • Mouse anti Pig CD25
(Rated 0.0 out of 5 based on 0 customer reviews)
  • Product Type
    Monoclonal Antibody
  • Clone
    K231.3B2
  • Isotype
    IgG1
1 Formats Available
    Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
    MCA1736GAC, Fdatasheet pdfdatasheet pdf0.1 mg
    MCA1736GA
    Summary
    Secondary Antibodies
    Negative Isotype Controls
    Useful Reagents
    Positive Controls
    Histology Controls
    More Images
    References
    Reviews
    -
    • Mouse anti Pig CD25, clone K231.3B2 recognizes porcine CD25, the alpha chain of the interleukin 2 receptor (IL-2Rα), also known as the low affinity Interleukin 2 receptor. The IL-2 receptor exists in three forms, the high affinity heterodimer, the intermediate affinity β monomer and the low affinity α monomer configurations. Clone K231.3B2 was clustered as CD25 at the First International Workshop to Define Swine Cluster of Differentiation (CD) Antigens (Lunney et al. 1994).

      Mouse anti pig CD25, clone K231.3B2 immunoprecipitates a protein of ~65-70 kDa from activated lymphocyte preparations (Bailey et al. 1992).

      CD25 is a 270 amino acid single pass type I transmembrane glycoprotein containing 2 Sushi domains. Low expression of CD25 is seen on resting peripheral blood mononuclear cells, rapidly up-regulated following stimulation by concanavalin A and phorbol myristate acetate, indicative of its role as an activation antigen (Bullido et al. 1999).
    • Intended Use
    • Target Species
      Pig
    • Product Form
      Purified IgG - liquid
    • Reconstitution
    • Preparation
      Purified IgG prepared by affinity chromatography on Protein A from tissue culture supernatant
    • Preservative Stabilisers
      0.09% Sodium Azide (NaN3)
    • Immunogen
      Con A activated porcine peripheral blood lymphocytes.
    • Purity
    • Approx. Protein Concentrations
      IgG concentration 1.0 mg/ml
    • Reagents In The Kit
    • Preparing The Antibody
    • Test Principle
    • Buffer Solution
      Phosphate buffered saline
    • Fusion Partners
      Spleen cells from immunised mice were fused with cells of the mouse P3-X63-Ag.8.653 myeloma cell line.
    • Storage
      Store at +4oC or at -20oC if preferred.
      Storage in frost-free freezers is not recommended.
      This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
    • Shelf Life
      18 months from date of despatch
    • GO Terms
      receptor activity
      integral to membrane
    • UniProt
    • Entrez Gene
    • Acknowledgements
    • Regulatory
      For research purposes only
    • This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.

    • Application NameYesNoMin DilutionMax Dilution
      Flow Cytometry1/251/200
      Immunohistology - Frozen

    • Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
    • Technical Advice
    • Recommended Protocol
    • Flow Cytometry
      Use 10ul of the suggested working dilution to label 1x106 cells in 100ul
    • ELISA
    • Immunohistology
    • Histology Positive Control Tissue
    • Immunofluorescence
    • Western Blotting
    • Instructions For Use

    Additional CD25 Antibody Formats

    Formats Clone Applications Sizes available
    CD25 Antibody : Purified K231.3B2 C, F 0.1 mg
    • Copyright © 2016 Bio-Rad Antibodies (formerly AbD Serotec)

    Recommended Secondary Antibody

      DescriptionProduct CodePack SizeApplicationsList PriceQuantity
      Goat anti Mouse IgG (H/L):Alk. Phos. (Multi Species Adsorbed)STAR117A0.5 mgE, WB
      STAR117A
      Goat anti Mouse IgG/A/M:Alk. Phos.STAR87A1 mgC, E, WB
      STAR87A
      Goat anti Mouse IgG (H/L):DyLight®488 (Multi Species Adsorbed)STAR117D488GA0.1 mgF, IF
      STAR117D488GA
      Goat anti Mouse IgG (H/L):DyLight®549 (Multi Species Adsorbed)STAR117D549GA0.1 mgF, IF
      STAR117D549GA
      Goat anti Mouse IgG (H/L):DyLight®649 (Multi Species Adsorbed)STAR117D649GA0.1 mgF, IF
      STAR117D649GA
      Goat anti Mouse IgG (H/L):DyLight®680 (Multi Species Adsorbed)STAR117D680GA0.1 mgF, WB
      STAR117D680GA
      Goat anti Mouse IgG (H/L):DyLight®800 (Multi Species Adsorbed)STAR117D800GA0.1 mgF, IF, WB
      STAR117D800GA
      Rabbit F(ab')2 anti Mouse IgG:Dylight®800STAR8D800GA0.1 mgF, IF, WB
      STAR8D800GA
      Goat anti Mouse IgG (H/L):FITC (Multi Species Adsorbed)STAR117F0.5 mgF
      STAR117F
      Goat anti Mouse IgG:FITC (Rat Adsorbed)STAR700.5 mgF
      STAR70
      Goat anti Mouse IgG (Fc):FITCSTAR120F1 mgC, F
      STAR120F
      Rabbit F(ab')2 anti Mouse IgG:FITCSTAR9B1 mgF
      STAR9B
      Human anti Mouse IgG1:HRPHCA036P0.1 mgE
      HCA036P
      Goat anti Mouse IgG (H/L):HRP (Multi Species Adsorbed)STAR117P0.5 mgE, WB
      STAR117P
      Goat anti Mouse IgG:HRP (Rat Adsorbed)STAR770.5 mgC, E, P
      STAR77
      Goat anti Mouse IgG (Fc):HRPSTAR120P1 mgE, WB
      STAR120P
      Rabbit F(ab')2 anti Mouse IgG:HRP (Human Adsorbed)STAR13B1 mgC, E, P, RE, WB
      STAR13B
      Goat anti Mouse IgG/A/M:HRP (Human Adsorbed)STAR87P1 mgE
      STAR87P
      Rabbit F(ab')2 anti Mouse IgG:RPESTAR12A1 mlF
      STAR12A
      Goat anti Mouse IgG:RPE (Rat Adsorbed)STAR761 mlF
      STAR76

      Recommended Negative Isotype Control

        DescriptionProduct CodePack SizeApplicationsList PriceQuantity
        Mouse IgG1 Negative ControlMCA928100 TestsF
        MCA928

        Useful Reagents

          DescriptionProduct CodePack SizeApplicationsList PriceQuantity
          Mouse anti Pig CD45:Alexa Fluor® 647MCA1222A647100 Tests/1mlF
          MCA1222A647
          Mouse anti Pig CD14:FITCMCA1218F0.1 mgF
          MCA1218F
          Mouse anti Pig wCD8 Alpha:FITCMCA1223F0.1 mgF
          MCA1223F
          Mouse anti Pig CD4 Alpha:FITCMCA1749F0.1 mgF
          MCA1749F
          Mouse anti Pig wCD8 Alpha:RPEMCA1223PE100 TestsF
          MCA1223PE
          Mouse anti Pig CD4 Alpha:RPEMCA1749PE100 TestsF
          MCA1749PE

          Recommended Positive Controls

            Histology Controls

              • Application NameReference Images
                Immunofluorescence

              References

              Further Reading

              Write your review

              You may also be interested in...

              You May Also Be Interested In...