CD14 antibody | VPM67

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Anti Sheep CD14 Antibody, clone VPM67 gallery image 1 — Sheep peripheral blood granulocytes stained with Mouse anti Sheep CD14 (**MCA5940GA**) followed by Goat anti Mouse IgG:FITC (STAR117F)

Mouse anti Sheep CD14

Product Type: Monoclonal Antibody
Clone: VPM67
Isotype: IgG1

Product Code	Applications	Datasheet	MSDS	Pack Size	List Price	Quantity
MCA5940GA	E F IP WB			0.1 mg

Mouse anti Sheep CD14 monoclonal antibody, clone VPM67 recognises ovine CD14, a GPI-anchored ~55 kDa membrane glycoprotein and monocyte/macrophage differentiation antigen, belonging to the lipopolysaccharide receptor family. Ovine CD14 is expressed by monocytes, macrophages and peripheral blood granulocytes (Hopkins et. al. 1996).

CD14 acts as a receptor for the potent bacterial endotoxin, lipopolysaccharide (LPS), facilitated by LPS-binding protein. The binding of LPS to CD14 results in cell activation, the release of cytokines and the inflammatory response, and has been shown to upregulate the cell surface expression of adhesion molecules.

Mouse anti Sheep CD14 monoclonal antibody, clone VPM67 has been shown to recognise CD14 in other domestic ruminant species including Water Buffalo (Bubalus bubalis) (Vimos et. al. 1996) and domestic cattle (Hopkins et. al. 1996). In addition to clone VPM67, clones VPM65 and VPM66 are also available.

Product Details

Target Species

Sheep

Species Cross-Reactivity

Target Species	Cross Reactivity
Bovine
Water Buffalo

N.B. Antibody reactivity and working conditions may vary between species.

Product Form

Purified IgG - liquid

Preparation

Purified IgG prepared by affinity chromatography on Protein A from tissue culture supernatant

Buffer Solution

Phosphate buffered saline

Preservative Stabilisers

0.09% Sodium Azide (NaN₃)

Carrier Free

Yes

Immunogen

Ovine macrophages.

Approx. Protein Concentrations

IgG concentration 1.0 mg/ml

Fusion Partners

Spleen cells from immunised BALB/c mice were fused with cells of the mouse NS0 myeloma cell line.

Storage Information

Storage: Store at +4^oC or at -20^oC if preferred.

This product should be stored undiluted.

Storage in frost free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Guarantee: 18 months from date of despatch.

More Information

UniProt: Q06AV9 Related reagents; Q95122 Related reagents
Entrez Gene: CD14 Related reagents
GO Terms: GO:0005886 plasma membrane; GO:0006954 inflammatory response; GO:0031225 anchored to membrane; GO:0045087 innate immune response
Regulatory: For research purposes only

Applications of CD14 antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.

Application Name	Min Dilution	Max Dilution
ELISA
Flow Cytometry	1/25	1/200
Immunoprecipitation
Western Blotting

Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.

Flow Cytometry: Use 10ul of the suggested working dilution to label 10⁶ cells or cells or 100ul whole blood.

Secondary Antibodies Available

Description	Product Code	Pack Size	Applications
Goat anti Mouse IgG (H/L):Alk. Phos. (Multi Species Adsorbed)	STAR117A	0.5 mg	E WB
Goat anti Mouse IgG (H/L):DyLight®488 (Multi Species Adsorbed)	STAR117D488GA	0.1 mg	F IF
Goat anti Mouse IgG (H/L):DyLight®680 (Multi Species Adsorbed)	STAR117D680GA	0.1 mg	F WB
Goat anti Mouse IgG (H/L):DyLight®800 (Multi Species Adsorbed)	STAR117D800GA	0.1 mg	F IF WB
Goat anti Mouse IgG (H/L):FITC (Multi Species Adsorbed)	STAR117F	0.5 mg	F
Goat anti Mouse IgG (H/L):HRP (Multi Species Adsorbed)	STAR117P	0.5 mg	E WB
Goat anti Mouse IgG (Fc):FITC	STAR120F	1 mg	C F
Goat anti Mouse IgG (Fc):HRP	STAR120P	1 mg	E WB
Rabbit F(ab')2 anti Mouse IgG:RPE	STAR12A	1 ml	F
Rabbit F(ab')2 anti Mouse IgG:HRP (Human Adsorbed)	STAR13B	1 mg	C E P RE WB
Goat anti Mouse IgG:FITC (Rat Adsorbed)	STAR70	0.5 mg	F
Goat anti Mouse IgG:RPE (Rat Adsorbed)	STAR76	1 ml	F
Goat anti Mouse IgG:HRP (Rat Adsorbed)	STAR77	0.5 mg	C E P
Goat anti Mouse IgG/A/M:Alk. Phos.	STAR87A	1 mg	C E WB
Goat anti Mouse IgG/A/M:HRP (Human Adsorbed)	STAR87P	1 mg	E
Rabbit F(ab')2 anti Mouse IgG:Dylight®800	STAR8D800GA	0.1 mg	F IF WB
Rabbit F(ab')2 anti Mouse IgG:FITC	STAR9B	1 mg	F

Useful Reagents Available

Description	Product Code	Pack Size	Applications
Mouse anti Sheep CD25:FITC	MCA2218F	0.1 mg	F
Mouse anti Sheep CD25	MCA2218GA	0.1 mg	C F IP
Mouse anti Sheep CD45:RPE	MCA2220PE	100 Tests	F

Product Specific References

References for CD14 antibody

Akesson, C.P. et al. (2008) Phenotypic characterisation of intestinal dendritic cells in sheep.
Dev Comp Immunol. 32 (7): 837-49.
Gupta, V.K. et al. (1996) Identification of the sheep homologue of the monocyte cell surface molecule--CD14.
Vet Immunol Immunopathol. 51 (1-2): 89-99.
Vilmos, P. et al. (1996) Phylogenetically conserved epitopes of leukocyte antigens.
Vet Immunol Immunopathol. 52 (4): 415-26.
Hopkins, J. & Gupta, V.K. (1996) Identification of three myeloid-specific differentiation antigens in sheep.
Vet Immunol Immunopathol. 52 (4): 329-39.
Sopp, P. et al. (1996) Identification of bovine CD14.
Vet Immunol Immunopathol. 52 (4): 323-8.

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