CD25 antibody | 9.14

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MCA2218GA
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C F IP 0.1 mg loader
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Mouse anti sheep CD25, clone 9.14 recognizes the ovine homologue of human CD25, also known as interleukin-2 receptor alpha chain (IL-2Rα), a single pass type I membrane protein expressed by activated ovine T-cells.

Ovine Interleukin-2 is a cytokine involved in the proliferation, growth and differentiation of T-cells, B-cells and NK cells and its receptor is composed of 3 subunits, an α chain (CD25), a β chain (CD122) and a γ chain (CD132). A non-covalent association of the α and β subunits is required to form the high affinity receptor for IL-2.

Mouse anti sheep CD25, clone 9.14 immunoprecipitates a band of ~47 kDa under reducing conditions, as expected for the mature protein due to high glycosylation, consistent with the observed molecular weight of IL-2Rα in other species including humans (Verhagen et. al.1993).

Antibodies to CD4, FoxP3 and CD25 may be used elucidate properties of T regulatory cells (T-regs), a unique subset of T helper cells that function in the control of effector cells vital in preventing autoimmunity (Rocchi et. al. 2011).

Mouse anti sheep CD25, clone 9.14 is one of a wide range of monoclonal antibodies available from Bio-Rad for ovine research and provides an important tool for the identification of ovine CD25.

Target Species
Sheep
Product Form
Purified IgG - liquid
Preparation
Purified IgG prepared by affinity chromatography on Protein A from tissue culture supernatant
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.09% Sodium Azide (NaN3)
Carrier Free
Yes
Approx. Protein Concentrations
IgG concentration 1.0 mg/ml
Fusion Partners
Spleen cells from immunised BALB/c mice were fused with cells of the mouse NS-1 myeloma cell line.
Regulatory
For research purposes only
Guarantee
12 months from date of despatch

This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.

Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Flow Cytometry 1/10 1/50
Immunohistology - Frozen
Immunoprecipitation
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
Flow Cytometry
Use 10ul of the suggested working dilution to label 1 x 106 cells in 100ul

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Source Reference

  1. Verhagen, A.M. et al. (1993) Characterization of the ovine interleukin-2 receptor-alpha chain: differential induction on precultured alpha beta and gamma delta T cells.
    Immunology. 79 (3): 471-8.

References for CD25 antibody

  1. Newland, A. et al. (2004) Ovine dendritic cells transduced with an adenoviral CTLA4eEGFP fusion protein construct induce hyporesponsiveness to allostimulation
    Immunology 113: 310-7.
  2. Gillan, S. et al. (2010) Identification of immune parameters to differentiate disease states among sheep infected with Mycobacterium avium subsp. paratuberculosis.
    Clin Vaccine Immunol. 17: 108-17.
  3. Gillan, S. et al. (2010) Ovine immune parameters following immunisation against Mycobacterium avium ssp. paratuberculosis using a lipid-based live-cell vaccine.
    Vet Immunol Immunopathol. 137 (1-2): 109-19.
  4. Piero, B. et al. (2016) Peripheral Blood and Milk Leukocytes Subsets of Lactating Sarda Ewes
    It J Anim Sci. 12 (2): e34.
  5. Willems, M.G. et al. (2016) Systemic interleukin-2 administration improves lung function and modulates chorioamnionitis-induced pulmonary inflammation in the ovine fetus.
    Am J Physiol Lung Cell Mol Physiol. 310 (1): L1-7.
  6. Lebedev, M. et al. (2021) Myeloid-like γδ T cell subset in the immune response to an experimental Rift Valley fever vaccine in sheep
    Veterinary Immunology and Immunopathology. : 110184.
  7. Curina, G. et al. (2018) Evaluation of immune responses in mice and sheep inoculated with a live attenuated Brucella melitensis. REV1 vaccine produced in bioreactor.
    Vet Immunol Immunopathol. 198: 44-53.
  8. Wooldridge, A.L. et al. (2019) Maternal allergic asthma during pregnancy alters fetal lung and immune development in sheep: potential mechanisms for programming asthma and allergy.
    J Physiol. 597 (16): 4251-62.
  9. View The Latest Product References

Further Reading

  1. Rocchi, M.S. et al. (2011) Identification of CD4+CD25 high Foxp3+ T cells in ovine peripheral blood.
    Vet Immunol Immunopathol. 144 (1-2): 172-7.

Flow Cytometry

Synonyms
IL-2R Alpha Chain
UniProt
P26898
Entrez Gene
IL2RA
GO Terms
GO:0016021 integral to membrane
GO:0004872 receptor activity
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Please Note: All Products are "FOR RESEARCH PURPOSES ONLY"

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