CD45 antibody | 1.11.32

Mouse anti Sheep CD45:RPE

Product Type
Monoclonal Antibody
Clone
1.11.32
Isotype
IgG1
Specificity
CD45

Product Code Applications Pack Size List Price Your Price Qty
MCA2220PE
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Mouse anti Sheep CD45 antibody, clone 1.11.32 recognizes the ovine CD45 (Leucocyte common antigen), expressed on all ovine lymphocytes, macrophages and granulocytes. Mouse anti Sheep CD45 antibody, clone 1.11.32 immunoprecipitates CD45 molecules of 190 kDa, 210 kDa and 225 kDa from lymph node lysates.

Target Species
Sheep
Species Cross-Reactivity
Target SpeciesCross Reactivity
Bovine
Goat
N.B. Antibody reactivity and working conditions may vary between species.
Product Form
Purified IgG conjugated to R. Phycoerythrin (RPE) - lyophilized
Reconstitution
Reconstitute with 1 ml distilled water
Preparation
Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.09%Sodium Azide
1%Bovine Serum Albumin
5%Sucrose
Immunogen
Ovine efferent lymphatic duct lymphocytes.
Fusion Partners
Spleen cells from immunised BALB/c mice were fused with cells of the mouse NS-1 myeloma cell line.
Max Ex/Em
Fluorophore Excitation Max (nm) Emission Max (nm)
RPE 488nm laser 496 578
Regulatory
For research purposes only
Guarantee
12 months from date of despatch

Prior to reconstitution store at +4oC. Following reconstitution store at +4oC.

DO NOT FREEZE.

This product should be stored undiluted. This product is photosensitive and should be protected from light. Should this product contain a precipitate we recommend microcentrifugation before use.

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Flow Cytometry Neat
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
Flow Cytometry
Use 10ul of the suggested working dilution to label 106 cells in 100ul.

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Description Product Code Applications Pack Size List Price Your Price Quantity
Mouse IgG1 Negative Control:RPE MCA928PE F 100 Tests loader
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Description Mouse IgG1 Negative Control:RPE

Source Reference

  1. Maddox, J.F. et al. (1985) The sheep analogue of leucocyte common antigen (LCA).
    Immunology. 55 (2): 347-53.

References for CD45 antibody

  1. Mackay, C.R. et al. (1987) A monoclonal antibody to the p220 component of sheep LCA identifies B cells and a unique lymphocyte subset.
    Cell Immunol. 110 (1): 46-55.
  2. Zannettino, A.C. et al. (2010) Comparative assessment of the osteoconductive properties of different biomaterials in vivo seeded with human or ovine mesenchymal stem/stromal cells.
    Tissue Eng Part A. 16 (12): 3579-87.
  3. Mackay, C.R. et al. (1989) Gamma/delta T cells express a unique surface molecule appearing late during thymic development.
    Eur J Immunol. 19 (8): 1477-83.
  4. Breugelmans, S. et al. (2011) Immunoassay of lymphocyte subsets in ovine palatine tonsils.
    Acta Histochem. 113 (4): 416-22.
  5. Breugelmans, S. et al. (2011) Differences between the ovine tonsils based on an immunohistochemical quantification of the lymphocyte subpopulations.
    Comp Immunol Microbiol Infect Dis. 34: 217-25.
  6. Herdrich, B.J. et al. (2010) Regenerative healing following foetal myocardial infarction.
    Eur J Cardiothorac Surg. 38: 691-8.
  7. Reichert, J.C. et al. (2010) Ovine bone- and marrow-derived progenitor cells and their potential for scaffold-based bone tissue engineering applications in vitro and in vivo.
    J Tissue Eng Regen Med. 4: 565-76.
  8. Galinsky, R. et al. (2011) Effect of intra-amniotic lipopolysaccharide on nephron number in preterm fetal sheep.
    Am J Physiol Renal Physiol. 301 (2): F280-5.
  9. View The Latest Product References
  10. Kallapur, S.G. et al. (2011) Pulmonary and systemic inflammatory responses to intra-amniotic IL-1α in fetal sheep.
    Am J Physiol Lung Cell Mol Physiol. 301 (3): L285-95.
  11. Stickler, P. et al. (2010) Cyclically stretching developing tissue in vivo enhances mechanical strength and organization of vascular grafts.
    Acta Biomater. 6 (7): 2448-56.
  12. Berardinelli, P. et al. (2013) Role of amniotic fluid mesenchymal cells engineered on MgHA/collagen-based scaffold allotransplanted on an experimental animal study of sinus augmentation.
    Clin Oral Investig. 17 (7): 1661-75.
  13. Geherin, S.A. et al. (2012) The skin, a novel niche for recirculating B cells.
    J Immunol. 188: 6027-35.
  14. Jahroomishirazi, R. et al. (2014) Isolation and Characterization of CD271+ Stem Cells Derived from Sheep Dermal Skin.
    Cells Tissues Organs. 200 (2): 141-52.
  15. Stephens, E.H. et al. (2016) Extracellular matrix remodeling in wound healing of critical size defects in the mitral valve leaflet.
    Heart Vessels. 31 (7): 1186-95.
  16. Mokhtari, S. et al. (2016) Boosting Hematopoietic Engraftment after in Utero Transplantation through Vascular Niche Manipulation.
    Stem Cell Reports. 6 (6): 957-69.
  17. Bischoff, J. et al. (2016) CD45 Expression in Mitral Valve Endothelial Cells After Myocardial Infarction.
    Circ Res. 119 (11): 1215-25.
  18. Lydon, H. et al. (2018) Peripheral mononuclear blood cell apheresis in a preclinical ovine model.
    BMC Vet Res. 14 (1): 47.
  19. Schwarz, E.R. et al. (2020) Experimental Infection of Mid-Gestation Pregnant Female and Intact Male Sheep with Zika Virus.
    Viruses. 12 (3)Mar 07 [Epub ahead of print].
  20. Barboni, B. et al. (2013) Synthetic bone substitute engineered with amniotic epithelial cells enhances bone regeneration after maxillary sinus augmentation.
    PLoS One. 8 (5): e63256.
  21. Ramm, R. et al. (2020) Decellularization combined with enzymatic removal of N-linked glycans and residual DNA reduces inflammatory response and improves performance of porcine xenogeneic pulmonary heart valves in an ovine in vivo model.
    Xenotransplantation. 27 (2): e12571.
  22. López-Fernández, A. et al. (2020) Effect of Allogeneic Cell-Based Tissue-Engineered Treatments in a Sheep Osteonecrosis Model.
    Tissue Eng Part A. 26 (17-18): 993-1004.
  23. Wooldridge, A.L. et al. (2019) Maternal allergic asthma during pregnancy alters fetal lung and immune development in sheep: potential mechanisms for programming asthma and allergy.
    J Physiol. 597 (16): 4251-4262.
  24. Savy, V. et al. (2021) Effect of Embryo Aggregation on In Vitro Development of Adipose-Derived Mesenchymal Stem Cell-Derived Bovine Clones.
    Cell Reprogram. 23 (5): 277-89.

Synonyms
LCA
RRID
AB_324751
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Please Note: All Products are "FOR RESEARCH PURPOSES ONLY"

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