CD25 antibody | 9.14
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|Mouse anti sheep CD25, clone 9.14 recognizes the ovine homologue of human CD25, also known as interleukin-2 receptor alpha chain (IL-2Rα), a single pass type I membrane protein expressed by activated ovine T-cells.
Ovine Interleukin-2 is a cytokine involved in the proliferation, growth and differentiation of T-cells, B-cells and NK cells and its receptor is composed of 3 subunits, an α chain (CD25), a β chain (CD122) and a γ chain (CD132). A non-covalent association of the α and β subunits is required to form the high affinity receptor for IL-2.
Mouse anti sheep CD25, clone 9.14 immunoprecipitates a band of ~47 kDa under reducing conditions, as expected for the mature protein due to high glycosylation, consistent with the observed molecular weight of IL-2Rα in other species including humans (Verhagen et. al.1993).
Antibodies to CD4, FoxP3 and CD25 may be used elucidate properties of T regulatory cells (T-regs), a unique subset of T helper cells that function in the control of effector cells vital in preventing autoimmunity (Rocchi et. al. 2011).
Mouse anti sheep CD25, clone 9.14 is one of a wide range of monoclonal antibodies available from Bio-Rad for ovine research and provides an important tool for the identification of ovine CD25.
- Target Species
- Product Form
- Purified IgG conjugated to Fluorescein Isothiocyanate Isomer 1 (FITC) - liquid
- Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
0.09% Sodium Azide 1% Bovine Serum Albumin
- Approx. Protein Concentrations
- IgG concentration 0.1 mg/ml
- Fusion Partners
- Spleen cells from immunised BALB/c mice were fused with cells of the mouse NS-1 myeloma cell line.
- Max Ex/Em
Fluorophore Excitation Max (nm) Emission Max (nm) FITC 490 525
- For research purposes only
- 12 months from date of despatch
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended. This product is photosensitive and should be protected from light.
|Application Name||Verified||Min Dilution||Max Dilution|
- Flow Cytometry
- Use 10ul of the suggested working dilution to label 1 x 106 cells in 100ul.
Verhagen, A.M. et al. (1993) Characterization of the ovine interleukin-2 receptor-alpha chain: differential induction on precultured alpha beta and gamma delta T cells.
Immunology. 79 (3): 471-8.
References for CD25 antibody
Newland, A. et al. (2004) Ovine dendritic cells transduced with an adenoviral CTLA4eEGFP fusion protein construct induce hyporesponsiveness to allostimulation
Immunology 113: 310-7.
Gillan, S. et al. (2010) Identification of immune parameters to differentiate disease states among sheep infected with Mycobacterium avium subsp. paratuberculosis.
Clin Vaccine Immunol. 17: 108-17.
Gillan, S. et al. (2010) Ovine immune parameters following immunisation against Mycobacterium avium ssp. paratuberculosis using a lipid-based live-cell vaccine.
Vet Immunol Immunopathol. 137 (1-2): 109-19.
Piero, B. et al. (2016) Peripheral Blood and Milk Leukocytes Subsets of Lactating Sarda Ewes
It J Anim Sci. 12 (2): e34.
Willems, M.G. et al. (2016) Systemic interleukin-2 administration improves lung function and modulates chorioamnionitis-induced pulmonary inflammation in the ovine fetus.
Am J Physiol Lung Cell Mol Physiol. 310 (1): L1-7.
Lebedev, M. et al. (2021) Myeloid-like γδ T cell subset in the immune response to an experimental Rift Valley fever vaccine in sheep
Veterinary Immunology and Immunopathology. : 110184.
Curina, G. et al. (2018) Evaluation of immune responses in mice and sheep inoculated with a live attenuated Brucella melitensis. REV1 vaccine produced in bioreactor.
Vet Immunol Immunopathol. 198: 44-53.
Wooldridge, A.L. et al. (2019) Maternal allergic asthma during pregnancy alters fetal lung and immune development in sheep: potential mechanisms for programming asthma and allergy.
J Physiol. 597 (16): 4251-62.
View The Latest Product References
Rocchi, M.S. et al. (2011) Identification of CD4+CD25 high Foxp3+ T cells in ovine peripheral blood.
Vet Immunol Immunopathol. 144 (1-2): 172-7.
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