PCNA antibody | PC10

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MCA1558
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C F * IP P WB 0.1 mg loader
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Mouse anti PCNA antibody, clone PC10 recognizes the proliferating cell nuclear antigen, also known as PCNA or cyclin. PCNA is a 261 amino acid ~28 kDa nuclear protein vital for cellular DNA synthesis at the replication fork (Li et al. 1995) through its interaction with FEN1 (Wu et al. 1996). PCNA is the auxilliary protein for DNA polymerase δ (Bravo et al. 1987).

PCNA is highly conserved between mammalian species and other vertebrates. Mouse anti PCNA antibody, clone PC10 has been used for the detection of PCNA in a number of species including human, rat, mouse (Park et al. 2008), chicken (Franz-Odendaal 2008) and abalone (Harris et al. 2006).

Target Species
Rat
Species Cross-Reactivity
Target SpeciesCross Reactivity
Vertebrates Expected from Sequence
Invertebrates Expected from Sequence
Ferret
Chicken
Rabbit
Xenopus
Mouse
Horse
Sheep
Dog
Cat
Cynomolgus monkey
Rhesus Monkey
Hamster
Atlantic Salmon
Human
Bearded Dragon
Corn Snake
Nile Crocodile
N.B. Antibody reactivity and working conditions may vary between species.
Product Form
Purified IgG - liquid
Preparation
Antibody purified from tissue culture supernatant
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.09% sodium azide (NaN3)
Carrier Free
Yes
Immunogen
Rat PCNA made in the protein A expression vector pR1T2T
Approx. Protein Concentrations
IgG concentration 1.0 mg/ml
Regulatory
For research purposes only
Guarantee
12 months from date of despatch

This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.

Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Flow Cytometry 1 1/100
Immunohistology - Frozen
Immunohistology - Paraffin 1/50 1/100
Immunoprecipitation
Western Blotting 1/1000
  1. 1 Membrane permeabilization is required for this application. The use of Leucoperm (Product Code BUF09) is recommended for this purpose.
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
Flow Cytometry
Use 10μl of the suggested working dilution to label 1x106 cells in 100μl
Histology Positive Control Tissue
Tonsil

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References for PCNA antibody

  1. Garcia, R.L. et al. (1989) Analysis of proliferative grade using anti-PCNA/cyclin monoclonal antibodies in fixed, embedded tissues. Comparison with flow cytometric analysis.
    Am J Pathol. 134 (4): 733-9.
  2. Mathews, M.B. et al. (1984) Identity of the proliferating cell nuclear antigen and cyclin.
    Nature. 309 (5966): 374-6.
  3. Ogata, K. et al. (1985) Purification and N-terminal amino acid sequence of proliferating cell nuclear antigen (PCNA)/cyclin and development of ELISA for anti-PCNA antibodies.
    J Immunol. 135 (4): 2623-7.
  4. Jenkins, H. et al. (1993) Nuclei that lack a lamina accumulate karyophilic proteins and assemble a nuclear matrix.
    J Cell Sci. 106: 275-85.
  5. Landberg, G. et al. (1990) Flow cytometric multiparameter analysis of proliferating cell nuclear antigen/cyclin and Ki-67 antigen: a new view of the cell cycle.
    Exp Cell Res. 187 (1): 111-8.
  6. Wilson, G.D. et al. (1992) Flow cytometric characterisation of proliferating cell nuclear antigen using the monoclonal antibody PC10.
    Eur J Cancer. 28A (12): 2010-7.
  7. Prosperi, E. et al. (1993) Proliferating cell nuclear antigen complex formation induced by ultraviolet irradiation in human quiescent fibroblasts as detected by immunostaining and flow cytometry.
    Exp Cell Res. 205 (2): 320-5.
  8. Elsässer, H.P. et al. (1994) Growth of rat pancreatic acinar cells quantitated with a monoclonal antibody against the proliferating cell nuclear antigen.
    Cell Tissue Res. 276 (3): 603-9.
  9. View The Latest Product References
  10. Harris, L. et al. (2006) Characterisation of cell types in abalone (Haliotis spp.) tissues using immunohistochemical techniques
    Aquaculture 261: 1413-21
  11. Buggins, A.G. et al. (2001) Microenvironment produced by acute myeloid leukemia cells prevents T cell activation and proliferation by inhibition of NF-kappaB, c-Myc, and pRb pathways.
    J Immunol. 167: 6021-30.
  12. Kapitonova, M.Y. et al. (2010) Immunohistochemical characteristics of the hypophysis in normal conditions and chronic stress.
    Neurosci Behav Physiol. 40: 97-102.
  13. Franz-Odendaal, T.A. (2008) Toward understanding the development of scleral ossicles in the chicken, Gallus gallus.
    Dev Dyn. 237: 3240-51.
  14. Hashimoto, Y. et al. (2010) Rad51 protects nascent DNA from Mre11-dependent degradation and promotes continuous DNA synthesis.
    Nat Struct Mol Biol. 17: 1305-11.
  15. Fenton, M. et al. (2001) Cellular senescence after single and repeated balloon catheter denudations of rabbit carotid arteries.
    Arterioscler Thromb Vasc Biol. 21: 220-6.
  16. Park, J.H. et al. (2008) Gastric lesions and immune responses caused by long-term infection with Helicobacter heilmannii in C57BL/6 mice.
    J Comp Pathol. 139: 208-17.
  17. Izhak, L. et al. (2012) Dissecting the autocrine and paracrine roles of the CCR2-CCL2 axis in tumor survival and angiogenesis.
    PLoS One. 7: e28305.
  18. Di-poï, N. & Milinkovitch, M.C. (2016) The anatomical placode in reptile scale morphogenesis indicates shared ancestry among skin appendages in amniotes.
    Sci Adv. 2 (6): e1600708.
  19. Guzera, M. et al. (2016) In Vitro Influence of Mycophenolic Acid on Selected Parameters of Stimulated Peripheral Canine Lymphocytes.
    PLoS One. 11 (5): e0154429.
  20. Khlebnikov, V. V. et al. (2015) Developmental Characteristics of the Hypothalamo-Hypophyseal-Adrenal System in Chronic Heterotypical Stress
    Neuroscience and Behavioral Physiology. 46 (1): 100-5.
  21. Nakatsuka, M. & Kumabe, S (2018) Histological Alterations from Condyle Repositioning with Functional Appliances in Rats.
    J Clin Pediatr Dent. 42 (5): 391-7.
  22. Falbo, L. et al. (2020) SSRP1-mediated histone H1 eviction promotes replication origin assembly and accelerated development.
    Nat Commun. 11 (1): 1345.

Immunohistology - Paraffin

Synonyms
Proliferating Cell Nuclear Antigen
RRID
AB_324955
UniProt
P04961
Entrez Gene
Pcna
GO Terms
GO:0006260 DNA replication
GO:0043626 PCNA complex
GO:0006275 regulation of DNA replication
GO:0007507 heart development
GO:0046686 response to cadmium ion
GO:0019985 translesion synthesis
GO:0030337 DNA polymerase processivity factor activity
GO:0032077 positive regulation of deoxyribonuclease activity
GO:0033993 response to lipid
GO:0070557 PCNA-p21 complex
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Please Note: All Products are "FOR RESEARCH PURPOSES ONLY"

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