PCNA antibody | PC10

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Anti PCNA Antibody, clone PC10 thumbnail image 1

Anti PCNA Antibody, clone PC10 thumbnail image 2

Anti PCNA Antibody, clone PC10 thumbnail image 3

Anti PCNA Antibody, clone PC10 thumbnail image 4

Anti PCNA Antibody, clone PC10 thumbnail image 5

Anti PCNA Antibody, clone PC10 thumbnail image 6

Anti PCNA Antibody, clone PC10 gallery image 1 — Western blot analysis of HeLa Nuclear Extract probed with Mouse anti Human PCNA (**MCA1588**).

Anti PCNA Antibody, clone PC10 gallery image 2 — Staining of KM-H2 cells with Mouse anti PCNA:FITC (**MCA1558F**) permeabilized with Leucoperm (BUF09) and methanol.

Anti PCNA Antibody, clone PC10 gallery image 3 — K562 whole cell lysate was run under reducing conditions on SDS PAGE using the Bio-Rad V3 system and transferred to a PVDF membrane. Mouse anti Human PCNA (**MCA1558**) was used as primary antibody followed by a Goat anti Mouse IgG (H/L):HRP secondary antibody at a dilution of 1/10,000 (STAR207P) was used as the secondary antibody. Visualization was carried out using the Bio-Rad ChemiDoc with automatic exposure in grey scale.

Anti PCNA Antibody, clone PC10 gallery image 4 — K562 whole cell lysate was run in lane 2 under reducing conditions on SDS PAGE using the Bio-Rad V3 system and transferred to a PVDF membrane. Bio-Rad Precision Plus molecular weight marker was run in lane 1. **MCA1558P** Mouse anti Human PCNA:HRP conjugate was used as a direct label at a 1/1000 dilution. Visualization was carried out using the Bio-Rad ChemiDoc with automatic exposure in grey scale.

Anti PCNA Antibody, clone PC10 gallery image 5 — Multiplex staining of HeLa whole cell lysate with Mouse anti PCNA (**MCA1558**) detected with Goat anti Mouse IgG (H/L):DyLight®680 (STAR117D680GA) shown in red and Rabbit anti GAPDH (AHP1628) detected with Sheep anti Rabbit IgG:DyLight®488 (STAR36D488GA) shown in green

Anti PCNA Antibody, clone PC10 gallery image 6 — **Published customer image:**
Insert meta-description and applications
**Image caption:**
Development of epidermal scales during reptilian embryogenesis.
(B) Anatomical placodes in *C. niloticus* (left panels), *P.vitticeps* (middle panels), and *P. guttatus*
From: Di-Poï N, Milinkovitch MC.
The anatomical placode in reptile scale morphogenesis indicates shared ancestry among skin appendages in amniotes.
Sci Adv. 2016 Jun 24;2(6):e1600708.
This image is from an open access article distributed under the terms of the Creative Commons Attribution License.

Mouse anti PCNA:FITC

Mouse anti PCNA:HRP

Mouse anti PCNA

Product Type: Monoclonal Antibody
Clone: PC10
Isotype: IgG2a

Product Code	Applications	Pack Size
MCA1558F	F*	0.1 mg
MCA1558P	WB	0.1 mg
MCA1558	C F* IP P WB	0.1 mg

Mouse anti PCNA antibody, clone PC10 recognizes the proliferating cell nuclear antigen, also known as PCNA or cyclin. PCNA is a 261 amino acid ~28 kDa nuclear protein vital for cellular DNA synthesis at the replication fork (Li et al. 1995) through its interaction with FEN1 (Wu et al. 1996). PCNA is the auxilliary protein for DNA polymerase δ (Bravo et al. 1987).

PCNA is highly conserved between mammalian species and other vertebrates. Clone PC10 has been used for the detection of PCNA in a number of species including human, rat, mouse (Park et al. 2008), chicken (Franz-Odendaal 2008) and abalone (Harris et al. 2006).

Product Details

Target Species

Rat

Species Cross-Reactivity

Target Species	Cross Reactivity
Vertebrates	Expected from Sequence
Invertebrates	Expected from Sequence
Ferret
Chicken
Rabbit
Xenopus
Mouse
Horse
Sheep
Dog
Cat
Cynomolgus monkey
Rhesus Monkey
Hamster
Atlantic Salmon
Human

N.B. Antibody reactivity and working conditions may vary between species.

Product Form

Purified IgG conjugated to Fluorescein Isothiocyanate Isomer 1 (FITC) - liquid

Product Form

Purified IgG conjugated to Horseradish Peroxidase (HRP) - liquid

Product Form

Purified IgG - liquid

Preparation

Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant

Preparation

Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant

Buffer Solution

Phosphate buffered saline

Buffer Solution

Phosphate buffered saline

Buffer Solution

Phosphate buffered saline

Preservative Stabilisers

0.09%	Sodium Azide
1%	Bovine Serum Albumin

Preservative Stabilisers

0.01% Thiomersal
HRP Stabiliser (BUF052A)

Preservative Stabilisers

0.09%

Sodium Azide

Carrier Free

Yes

Immunogen

Rat PCNA made in the protein A expression vector pR1T2T

Approx. Protein Concentrations

IgG concentration 0.1 mg/ml

Approx. Protein Concentrations

IgG concentration 1.0 mg/ml

Approx. Protein Concentrations

IgG concentration 1.0 mg/ml

Storage Information

Storage: Store at +4^oC or at -20^oC if preferred.

This product should be stored undiluted.

Storage in frost-free freezers is not recommended. This product is photosensitive and should be protected from light.

Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Storage: Store at +4^oC. DO NOT FREEZE.
This product should be stored undiluted. This product is photosensitive and should be protected from light. Should this product contain a precipitate we recommend microcentrifugation before use.
Storage: Store at +4^oC or at -20^oC if preferred.

This product should be stored undiluted.

Storage in frost-free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Guarantee: 18 months from date of despatch.
Guarantee: 18 months from date of despatch
Guarantee: 18 months from date of despatch.

More Information

UniProt: P04961 Related reagents
Entrez Gene: Pcna Related reagents
GO Terms: GO:0006260 DNA replication; GO:0043626 PCNA complex; GO:0006275 regulation of DNA replication; GO:0007507 heart development; GO:0046686 response to cadmium ion; GO:0019985 translesion synthesis; GO:0030337 DNA polymerase processivity factor activity; GO:0032077 positive regulation of deoxyribonuclease activity; GO:0033993 response to lipid; GO:0070557 PCNA-p21 complex
Regulatory: For research purposes only

Applications of PCNA antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.

Application Name	Min Dilution	Max Dilution
Flow Cytometry ¹	Neat	1/10
Western Blotting		1/1000
Flow Cytometry ¹		1/100
Immunohistology - Frozen
Immunohistology - Paraffin	1/50	1/100
Immunoprecipitation
Western Blotting		1/1000

¹ Membrane permeabilisation is required for this application. Bio-Rad recommends the use of Leucoperm™ (Product Code BUF09) for this purpose.

¹ Membrane permeabilisation is required for this application. Bio-Rad recommends the use of Leucoperm™ (Product Code BUF09) for this purpose.

Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.

Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.

Flow Cytometry: Use 10ul of the suggested working dilution to label 1x10⁶ cells in 100ul.
Flow Cytometry: Use 10ul of the suggested working dilution to label 1x10⁶ cells in 100ul.
Immunohistology: This product does not require protein digestion pre-treatment of paraffin sections. This product does not require antigen retrieval using heat pre-treatment prior to staining of paraffin sections.
Histology Positive Control Tissue: Tonsil

Secondary Antibodies Available

Description	Product Code	Pack Size	Applications
Human anti Mouse IgG2a:FITC	HCA037F	0.1 mg	F
Human anti Mouse IgG2a:HRP	HCA037P	0.1 mg	E
Goat anti Mouse IgG (H/L):Alk. Phos. (Multi Species Adsorbed)	STAR117A	0.5 mg	E WB
Goat anti Mouse IgG (H/L):DyLight®488 (Multi Species Adsorbed)	STAR117D488GA	0.1 mg	F IF
Goat anti Mouse IgG (H/L):DyLight®680 (Multi Species Adsorbed)	STAR117D680GA	0.1 mg	F WB
Goat anti Mouse IgG (H/L):DyLight®800 (Multi Species Adsorbed)	STAR117D800GA	0.1 mg	F IF WB
Goat anti Mouse IgG (H/L):FITC (Multi Species Adsorbed)	STAR117F	0.5 mg	F
Goat anti Mouse IgG (H/L):HRP (Multi Species Adsorbed)	STAR117P	0.5 mg	E WB
Goat anti Mouse IgG (Fc):FITC	STAR120F	1 mg	C F
Goat anti Mouse IgG (Fc):HRP	STAR120P	1 mg	E WB
Rabbit F(ab')2 anti Mouse IgG:RPE	STAR12A	1 ml	F
Rabbit F(ab')2 anti Mouse IgG:HRP (Human Adsorbed)	STAR13B	1 mg	C E P RE WB
Goat anti Mouse IgG:FITC (Rat Adsorbed)	STAR70	0.5 mg	F
Goat anti Mouse IgG:RPE (Rat Adsorbed)	STAR76	1 ml	F
Goat anti Mouse IgG:HRP (Rat Adsorbed)	STAR77	0.5 mg	C E P
Goat anti Mouse IgG/A/M:Alk. Phos.	STAR87A	1 mg	C E WB
Goat anti Mouse IgG/A/M:HRP (Human Adsorbed)	STAR87P	1 mg	E
Rabbit F(ab')2 anti Mouse IgG:Dylight®800	STAR8D800GA	0.1 mg	F IF WB
Rabbit F(ab')2 anti Mouse IgG:FITC	STAR9B	1 mg	F

Negative Isotype Controls Available

Description	Product Code	Pack Size	Applications	List Price	Quantity
Mouse IgG2a Negative Control:FITC	MCA1210F	0.1 mg	F
Mouse IgG2a Negative Control	MCA1210	100 Tests	F

Application Based External Images

Immunohistology - Paraffin

Product Specific References

References for PCNA antibody

Mathews, M.B. et al. (1984) Identity of the proliferating cell nuclear antigen and cyclin.
Nature. 309 (5966): 374-6.
Ogata, K. et al. (1985) Purification and N-terminal amino acid sequence of proliferating cell nuclear antigen (PCNA)/cyclin and development of ELISA for anti-PCNA antibodies.
J Immunol. 135 (4): 2623-7.
Garcia, R.L. et al. (1989) Analysis of proliferative grade using anti-PCNA/cyclin monoclonal antibodies in fixed, embedded tissues. Comparison with flow cytometric analysis.
Am J Pathol. 134 (4): 733-9.
Landberg, G. et al. (1990) Flow cytometric multiparameter analysis of proliferating cell nuclear antigen/cyclin and Ki-67 antigen: a new view of the cell cycle.
Exp Cell Res. 187 (1): 111-8.
Wilson, G.D. et al. (1992) Flow cytometric characterisation of proliferating cell nuclear antigen using the monoclonal antibody PC10.
Eur J Cancer. 28A (12): 2010-7.
Prosperi, E. et al. (1993) Proliferating cell nuclear antigen complex formation induced by ultraviolet irradiation in human quiescent fibroblasts as detected by immunostaining and flow cytometry.
Exp Cell Res. 205 (2): 320-5.
Elsässer, H.P. et al. (1994) Growth of rat pancreatic acinar cells quantitated with a monoclonal antibody against the proliferating cell nuclear antigen.
Cell Tissue Res. 276 (3): 603-9.
Harris, L. et al. (2006) Characterisation of cell types in abalone (Haliotis spp.) tissues using immunohistochemical techniques
Aquaculture 261: 1413-21
Buggins, A.G. et al. (2001) Microenvironment produced by acute myeloid leukemia cells prevents T cell activation and proliferation by inhibition of NF-kappaB, c-Myc, and pRb pathways.
J Immunol. 167: 6021-30.
Kapitonova, M.Y. et al. (2010) Immunohistochemical characteristics of the hypophysis in normal conditions and chronic stress.
Neurosci Behav Physiol. 40: 97-102.
Franz-Odendaal, T.A. (2008) Toward understanding the development of scleral ossicles in the chicken, Gallus gallus.
Dev Dyn. 237: 3240-51.
Hashimoto, Y. et al. (2010) Rad51 protects nascent DNA from Mre11-dependent degradation and promotes continuous DNA synthesis.
Nat Struct Mol Biol. 17: 1305-11.
Jenkins, H. et al. (1993) Nuclei that lack a lamina accumulate karyophilic proteins and assemble a nuclear matrix.
J Cell Sci. 106: 275-85.
Park, J.H. et al. (2008) Gastric lesions and immune responses caused by long-term infection with Helicobacter heilmannii in C57BL/6 mice.
J Comp Pathol. 139: 208-17.
Izhak, L. et al. (2012) Dissecting the autocrine and paracrine roles of the CCR2-CCL2 axis in tumor survival and angiogenesis.
PLoS One. 7: e28305.
Fenton, M. et al. (2001) Cellular senescence after single and repeated balloon catheter denudations of rabbit carotid arteries.
Arterioscler Thromb Vasc Biol. 21: 220-6.
Guzera, M. et al. (2016) In Vitro Influence of Mycophenolic Acid on Selected Parameters of Stimulated Peripheral Canine Lymphocytes.
PLoS One. 11 (5): e0154429.
Khlebnikov, V. V. et al. (2015) Developmental Characteristics of the Hypothalamo-Hypophyseal-Adrenal System in Chronic Heterotypical Stress
Neuroscience and Behavioral Physiology. 46 (1): 100-5.
Nakatsuka, M. & Kumabe, S. (2018) Histological Alterations from Condyle Repositioning with Functional Appliances in Rats.
J Clin Pediatr Dent. May 15 [Epub ahead of print].
Di-poï, N. & Milinkovitch, M.C. (2016) The anatomical placode in reptile scale morphogenesis indicates shared ancestry among skin appendages in amniotes.
Sci Adv. 2 (6): e1600708.

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