PCNA antibody | PC10
Mouse anti PCNA antibody, clone PC10 (MCA1588) used to label PCNA expressing cells by immunofluorescence on reptilian embryonic sections.
Image caption:
Development of epidermal scales during reptilian embryogenesis.
(B) Anatomical placodes in C. niloticus (left panels), P.vitticeps (middle panels), and P. guttatus
From: Di-Poï N, Milinkovitch MC.
The anatomical placode in reptile scale morphogenesis indicates shared ancestry among skin appendages in amniotes.
Sci Adv. 2016 Jun 24;2(6):e1600708.
This image is from an open access article distributed under the terms of the Creative Commons Attribution License.
Mouse anti PCNA antibody, clone PC10 (MCA1558) used to evaluate PCNA abundance in Xenopus lysates by western blotting.
Image caption:
SSRP1 N-Terminal domain dependent stimulation of DNA replication through histone H1 chromatin eviction.
WB of chromatin isolated at 60 min after addition to interphase egg extracts of SN pre-incubated with 200 ng/μl recombinant SSRP1 or equimolar amounts of its mutant versions and probed with the indicated antibodies.
From: Falbo L, Raspelli E, Romeo F, Fiorani S, Pezzimenti F, Casagrande F, Costa I, Parazzoli D, Costanzo V.
SSRP1-mediated histone H1 eviction promotes replication origin assembly and accelerated development.
Nat Commun. 2020 Mar 12;11(1):1345.
doi: 10.1038/s41467-020-15180-5.
This image is from an open access article distributed under terms of a Creative Commons Attribution License.
Filter by Application:
WB F IF ResetMouse anti PCNA
- Product Type
- Monoclonal Antibody
- Clone
- PC10
- Isotype
- IgG2a
- Specificity
- PCNA
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Antibody Quality and 
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| Mouse anti PCNA antibody, clone PC10 recognizes the proliferating cell nuclear antigen, also known as PCNA or cyclin. PCNA is a 261 amino acid ~28 kDa nuclear protein vital for cellular DNA synthesis at the replication fork (Li et al. 1995) through its interaction with FEN1 (Wu et al. 1996). PCNA is the auxilliary protein for DNA polymerase δ (Bravo et al. 1987). PCNA is highly conserved between mammalian species and other vertebrates. Mouse anti PCNA antibody, clone PC10 has been used for the detection of PCNA in a number of species including human, rat, mouse (Park et al. 2008), chicken (Franz-Odendaal 2008) and abalone (Harris et al. 2006). |
- Target Species
- Rat
- Species Cross-Reactivity
-
Target Species Cross Reactivity Vertebrates Expected from Sequence Invertebrates Expected from Sequence Ferret Chicken Rabbit Xenopus Mouse Horse Sheep Dog Cat Cynomolgus monkey Rhesus Monkey Hamster Atlantic Salmon Human Bearded Dragon Corn Snake Nile Crocodile - N.B. Antibody reactivity and working conditions may vary between species.
- Product Form
- Purified IgG - liquid
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
0.09% Sodium Azide - Carrier Free
- Yes
- Immunogen
- Rat PCNA made in the protein A expression vector pR1T2T
- Approx. Protein Concentrations
- IgG concentration 1.0 mg/ml
- Regulatory
- For research purposes only
- Guarantee
- 12 months from date of despatch
This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
| Application Name | Verified | Min Dilution | Max Dilution |
|---|---|---|---|
| Flow Cytometry 1 |  |
1/100 | |
| Immunohistology - Frozen | |
||
| Immunohistology - Paraffin | |
1/50 | 1/100 |
| Immunoprecipitation | |
||
| Western Blotting | |
1/1000 |
- 1 Membrane permeabilisation is required for this application. Bio-Rad recommends the use of Leucoperm™ (Product Code BUF09) for this purpose.
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
- Flow Cytometry
- Use 10ul of the suggested working dilution to label 1x106 cells in 100ul.
- Immunohistology
- This product does not require protein digestion pre-treatment of paraffin sections. This product does not require antigen retrieval using heat pre-treatment prior to staining of paraffin sections.
- Histology Positive Control Tissue
- Tonsil
| Description | Product Code | Applications | Pack Size | List Price | Your Price | Quantity | |
|---|---|---|---|---|---|---|---|
| Mouse IgG2a Negative Control | MCA1210 | F | 100 Tests |
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| List Price | Your Price | ||||||
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| Description | Mouse IgG2a Negative Control | ||||||
References for PCNA antibody
-
Garcia, R.L. et al. (1989) Analysis of proliferative grade using anti-PCNA/cyclin monoclonal antibodies in fixed, embedded tissues. Comparison with flow cytometric analysis.
Am J Pathol. 134 (4): 733-9. -
Mathews, M.B. et al. (1984) Identity of the proliferating cell nuclear antigen and cyclin.
Nature. 309 (5966): 374-6. -
Ogata, K. et al. (1985) Purification and N-terminal amino acid sequence of proliferating cell nuclear antigen (PCNA)/cyclin and development of ELISA for anti-PCNA antibodies.
J Immunol. 135 (4): 2623-7. -
Jenkins, H. et al. (1993) Nuclei that lack a lamina accumulate karyophilic proteins and assemble a nuclear matrix.
J Cell Sci. 106: 275-85. -
Landberg, G. et al. (1990) Flow cytometric multiparameter analysis of proliferating cell nuclear antigen/cyclin and Ki-67 antigen: a new view of the cell cycle.
Exp Cell Res. 187 (1): 111-8. -
Wilson, G.D. et al. (1992) Flow cytometric characterisation of proliferating cell nuclear antigen using the monoclonal antibody PC10.
Eur J Cancer. 28A (12): 2010-7. -
Prosperi, E. et al. (1993) Proliferating cell nuclear antigen complex formation induced by ultraviolet irradiation in human quiescent fibroblasts as detected by immunostaining and flow cytometry.
Exp Cell Res. 205 (2): 320-5. -
Elsässer, H.P. et al. (1994) Growth of rat pancreatic acinar cells quantitated with a monoclonal antibody against the proliferating cell nuclear antigen.
Cell Tissue Res. 276 (3): 603-9.
View The Latest Product References
-
Harris, L. et al. (2006) Characterisation of cell types in abalone (Haliotis spp.) tissues using immunohistochemical techniques
Aquaculture 261: 1413-21 -
Buggins, A.G. et al. (2001) Microenvironment produced by acute myeloid leukemia cells prevents T cell activation and proliferation by inhibition of NF-kappaB, c-Myc, and pRb pathways.
J Immunol. 167: 6021-30. -
Kapitonova, M.Y. et al. (2010) Immunohistochemical characteristics of the hypophysis in normal conditions and chronic stress.
Neurosci Behav Physiol. 40: 97-102. -
Franz-Odendaal, T.A. (2008) Toward understanding the development of scleral ossicles in the chicken, Gallus gallus.
Dev Dyn. 237: 3240-51. -
Hashimoto, Y. et al. (2010) Rad51 protects nascent DNA from Mre11-dependent degradation and promotes continuous DNA synthesis.
Nat Struct Mol Biol. 17: 1305-11. -
Fenton, M. et al. (2001) Cellular senescence after single and repeated balloon catheter denudations of rabbit carotid arteries.
Arterioscler Thromb Vasc Biol. 21: 220-6. -
Park, J.H. et al. (2008) Gastric lesions and immune responses caused by long-term infection with Helicobacter heilmannii in C57BL/6 mice.
J Comp Pathol. 139: 208-17. -
Izhak, L. et al. (2012) Dissecting the autocrine and paracrine roles of the CCR2-CCL2 axis in tumor survival and angiogenesis.
PLoS One. 7: e28305. -
Di-poï, N. & Milinkovitch, M.C. (2016) The anatomical placode in reptile scale morphogenesis indicates shared ancestry among skin appendages in amniotes.
Sci Adv. 2 (6): e1600708. -
Guzera, M. et al. (2016) In Vitro Influence of Mycophenolic Acid on Selected Parameters of Stimulated Peripheral Canine Lymphocytes.
PLoS One. 11 (5): e0154429. -
Khlebnikov, V. V. et al. (2015) Developmental Characteristics of the Hypothalamo-Hypophyseal-Adrenal System in Chronic Heterotypical Stress
Neuroscience and Behavioral Physiology. 46 (1): 100-5. -
Nakatsuka, M. & Kumabe, S (2018) Histological Alterations from Condyle Repositioning with Functional Appliances in Rats.
J Clin Pediatr Dent. 42 (5): 391-7. -
Falbo, L. et al. (2020) SSRP1-mediated histone H1 eviction promotes replication origin assembly and accelerated development.
Nat Commun. 11 (1): 1345.
- Synonyms
- Proliferating Cell Nuclear Antigen
- RRID
- AB_324955
- UniProt
- P04961
- Entrez Gene
- Pcna
- GO Terms
- GO:0006260 DNA replication
- GO:0043626 PCNA complex
- GO:0006275 regulation of DNA replication
- GO:0007507 heart development
- GO:0046686 response to cadmium ion
- GO:0019985 translesion synthesis
- GO:0030337 DNA polymerase processivity factor activity
- GO:0032077 positive regulation of deoxyribonuclease activity
- GO:0033993 response to lipid
- View More GO Terms
- GO:0070557 PCNA-p21 complex
View more products with PCNA specificity
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