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PCNA antibody | PC10

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Anti PCNA Antibody, clone PC10 thumbnail image 1 Anti PCNA Antibody, clone PC10 thumbnail image 2 Anti PCNA Antibody, clone PC10 thumbnail image 3 Anti PCNA Antibody, clone PC10 thumbnail image 4 Anti PCNA Antibody, clone PC10 thumbnail image 5 Anti PCNA Antibody, clone PC10 thumbnail image 6
Anti PCNA Antibody, clone PC10 gallery image 1
Western blot analysis of HeLa nuclear extract probed with Mouse anti Human PCNA antibody, clone PC10 (MCA1588).
Anti PCNA Antibody, clone PC10 gallery image 2
Staining of KM-H2 cells with FITC conjugated Mouse anti PCNA antibody, clone PC10 (MCA1558F) permeabilized with Leucoperm (BUF09) and methanol.
Anti PCNA Antibody, clone PC10 gallery image 3
K562 whole cell lysate was run under reducing conditions on SDS PAGE using the Bio-Rad V3 system and transferred to a PVDF membrane. Mouse anti Human PCNA antibody, clone PC10 (MCA1558) was used as primary antibody followed by a Goat anti Mouse IgG (H/L):HRP secondary antibody at a dilution of 1/10,000 (STAR207P) was used as the secondary antibody. Visualization was carried out using the Bio-Rad ChemiDoc with automatic exposure in grey scale.
Anti PCNA Antibody, clone PC10 gallery image 4
K562 whole cell lysate was run in lane 2 under reducing conditions on SDS PAGE using the Bio-Rad V3 system and transferred to a PVDF membrane. Bio-Rad Precision Plus molecular weight marker was run in lane 1. HRP conjugated Mouse anti Human PCNA antibody, clone PC10 (MCA1558P) was used as a direct label at a 1/1000 dilution. Visualization was carried out using the Bio-Rad ChemiDoc with automatic exposure in grey scale.
Anti PCNA Antibody, clone PC10 gallery image 5
Multiplex staining of HeLa whole cell lysate with Mouse anti PCNA antibody, clone PC10 (MCA1558) detected with Goat anti Mouse IgG (H/L):DyLight 680 (STAR117D680GA) shown in red and Rabbit anti GAPDH (AHP1628) detected with Sheep anti Rabbit IgG:DyLight 488 (STAR36D488GA) shown in green
Anti PCNA Antibody, clone PC10 gallery image 6
Published customer image:
Mouse anti PCNA antibody, clone PC10 (MCA1588) used to label PCNA expressing cells by immunofluorescence on reptilian embryonic sections.
Image caption:
Development of epidermal scales during reptilian embryogenesis.
(B) Anatomical placodes in C. niloticus (left panels), P.vitticeps (middle panels), and P. guttatus
From: Di-Poï N, Milinkovitch MC.
The anatomical placode in reptile scale morphogenesis indicates shared ancestry among skin appendages in amniotes.
Sci Adv. 2016 Jun 24;2(6):e1600708.
This image is from an open access article distributed under the terms of the Creative Commons Attribution License.

Mouse anti PCNA:FITC

Mouse anti PCNA:HRP

Mouse anti PCNA

Product Type
Monoclonal Antibody
Clone
PC10
Isotype
IgG2a
Product Code Applications Pack Size List Price Quantity
MCA1558F
Datasheet
F*
SDS
0.1 mg loader

MCA1558P
Datasheet
WB
SDS
0.1 mg loader

MCA1558
Datasheet
C F* IP P WB
SDS
0.1 mg loader

Mouse anti PCNA antibody, clone PC10 recognizes the proliferating cell nuclear antigen, also known as PCNA or cyclin. PCNA is a 261 amino acid ~28 kDa nuclear protein vital for cellular DNA synthesis at the replication fork (Li et al. 1995) through its interaction with FEN1 (Wu et al. 1996). PCNA is the auxilliary protein for DNA polymerase δ (Bravo et al. 1987).

PCNA is highly conserved between mammalian species and other vertebrates. Mouse anti PCNA antibody, clone PC10 has been used for the detection of PCNA in a number of species including human, rat, mouse (Park et al. 2008), chicken (Franz-Odendaal 2008) and abalone (Harris et al. 2006).

Product Details

Target Species
Rat
Species Cross-Reactivity
Target SpeciesCross Reactivity
Vertebrates Expected from Sequence
Invertebrates Expected from Sequence
Ferret
Chicken
Rabbit
Xenopus
Mouse
Horse
Sheep
Dog
Cat
Cynomolgus monkey
Rhesus Monkey
Hamster
Atlantic Salmon
Human
Bearded Dragon
Corn Snake
Nile Crocodile
N.B. Antibody reactivity and working conditions may vary between species.
Product Form
Purified IgG conjugated to Fluorescein Isothiocyanate Isomer 1 (FITC) - liquid
Product Form
Purified IgG conjugated to Horseradish Peroxidase (HRP) - liquid
Product Form
Purified IgG - liquid
Preparation
Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
Preparation
Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
Buffer Solution
Phosphate buffered saline
Buffer Solution
Phosphate buffered saline
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.09%Sodium Azide
1%Bovine Serum Albumin
Preservative Stabilisers
0.01% Thiomersal
HRP Stabiliser (BUF052A)
Preservative Stabilisers
0.09%Sodium Azide
Carrier Free
Yes
Immunogen
Rat PCNA made in the protein A expression vector pR1T2T
Approx. Protein Concentrations
IgG concentration 0.1 mg/ml
Approx. Protein Concentrations
IgG concentration 1.0 mg/ml
Approx. Protein Concentrations
IgG concentration 1.0 mg/ml

Storage Information

Storage
This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.

Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended. This product is photosensitive and should be protected from light.
Storage
Store at +4oC. DO NOT FREEZE.
This product should be stored undiluted. This product is photosensitive and should be protected from light. Should this product contain a precipitate we recommend microcentrifugation before use.
Storage
This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.

Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.
Guarantee
12 months from date of despatch
Guarantee
12 months from date of despatch
Guarantee
12 months from date of despatch

More Information

UniProt
P04961
Entrez Gene
Pcna
GO Terms
GO:0006260 DNA replication
GO:0043626 PCNA complex
GO:0006275 regulation of DNA replication
GO:0007507 heart development
GO:0046686 response to cadmium ion
GO:0019985 translesion synthesis
GO:0030337 DNA polymerase processivity factor activity
GO:0032077 positive regulation of deoxyribonuclease activity
GO:0033993 response to lipid
GO:0070557 PCNA-p21 complex
Regulatory
For research purposes only

Applications of PCNA antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Flow Cytometry 1 Neat 1/10
Western Blotting 1/1000
Flow Cytometry 1 1/100
Immunohistology - Frozen
Immunohistology - Paraffin 1/50 1/100
Immunoprecipitation
Western Blotting 1/1000
  1. 1 Membrane permeabilisation is required for this application. Bio-Rad recommends the use of Leucoperm™ (Product Code BUF09) for this purpose.
  1. 1 Membrane permeabilisation is required for this application. Bio-Rad recommends the use of Leucoperm™ (Product Code BUF09) for this purpose.
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
Flow Cytometry
Use 10ul of the suggested working dilution to label 1x106 cells in 100ul.
Flow Cytometry
Use 10ul of the suggested working dilution to label 1x106 cells in 100ul.
Immunohistology
This product does not require protein digestion pre-treatment of paraffin sections. This product does not require antigen retrieval using heat pre-treatment prior to staining of paraffin sections.
Histology Positive Control Tissue
Tonsil
Copyright © 2021 Bio-Rad Antibodies (formerly AbD Serotec)

Secondary Antibodies Available

Description Product Code Applications Pack Size List Price Quantity
Human anti Mouse IgG2a:HRP HCA037P E 0.1 mg loader
Goat anti Mouse IgG (H/L):Alk. Phos. (Multi Species Adsorbed) STAR117A E WB 0.5 mg loader
Goat anti Mouse IgG (H/L):DyLight®488 (Multi Species Adsorbed) STAR117D488GA F IF 0.1 mg loader
Goat anti Mouse IgG (H/L):DyLight®550 (Multi Species Adsorbed) STAR117D550 F IF WB 0.1 mg loader
Goat anti Mouse IgG (H/L):DyLight®650 (Multi Species Adsorbed) STAR117D650 F IF 0.1 mg loader
Goat anti Mouse IgG (H/L):DyLight®680 (Multi Species Adsorbed) STAR117D680GA F WB 0.1 mg loader
Goat anti Mouse IgG (H/L):DyLight®800 (Multi Species Adsorbed) STAR117D800GA F IF WB 0.1 mg loader
Goat anti Mouse IgG (H/L):FITC (Multi Species Adsorbed) STAR117F F 0.5 mg loader
Goat anti Mouse IgG (H/L):HRP (Multi Species Adsorbed) STAR117P C E WB 0.5 mg loader
Goat anti Mouse IgG (Fc):FITC STAR120F C F 1 mg loader
Goat anti Mouse IgG (Fc):HRP STAR120P E WB 1 mg loader
Rabbit F(ab')2 anti Mouse IgG:RPE STAR12A F 1 ml loader
Rabbit F(ab')2 anti Mouse IgG:HRP (Human Adsorbed) STAR13B C E P RE WB 1 mg loader
Goat anti Mouse IgG:FITC (Rat Adsorbed) STAR70 F 0.5 mg loader
Goat anti Mouse IgG:RPE (Rat Adsorbed) STAR76 F 1 ml loader
Goat anti Mouse IgG:HRP (Rat Adsorbed) STAR77 C E P 0.5 mg loader
Goat anti Mouse IgG/A/M:Alk. Phos. STAR87A C E WB 1 mg loader
Goat anti Mouse IgG/A/M:HRP (Human Adsorbed) STAR87P E 1 mg loader
Rabbit F(ab')2 anti Mouse IgG:Dylight®800 STAR8D800GA F IF WB 0.1 mg loader
Rabbit F(ab')2 anti Mouse IgG:FITC STAR9B F 1 mg loader

Negative Isotype Controls Available

Description Product Code Applications Pack Size List Price Quantity
Mouse IgG2a Negative Control:FITC MCA1210F F 0.1 mg loader
Mouse IgG2a Negative Control MCA1210 F 100 Tests loader

Application Based External Images

Immunohistology - Paraffin

Product Specific References

References for PCNA antibody

  1. Mathews, M.B. et al. (1984) Identity of the proliferating cell nuclear antigen and cyclin.
    Nature. 309 (5966): 374-6.
  2. Ogata, K. et al. (1985) Purification and N-terminal amino acid sequence of proliferating cell nuclear antigen (PCNA)/cyclin and development of ELISA for anti-PCNA antibodies.
    J Immunol. 135 (4): 2623-7.
  3. Jenkins, H. et al. (1993) Nuclei that lack a lamina accumulate karyophilic proteins and assemble a nuclear matrix.
    J Cell Sci. 106: 275-85.
  4. Landberg, G. et al. (1990) Flow cytometric multiparameter analysis of proliferating cell nuclear antigen/cyclin and Ki-67 antigen: a new view of the cell cycle.
    Exp Cell Res. 187 (1): 111-8.
  5. Wilson, G.D. et al. (1992) Flow cytometric characterisation of proliferating cell nuclear antigen using the monoclonal antibody PC10.
    Eur J Cancer. 28A (12): 2010-7.
  6. Prosperi, E. et al. (1993) Proliferating cell nuclear antigen complex formation induced by ultraviolet irradiation in human quiescent fibroblasts as detected by immunostaining and flow cytometry.
    Exp Cell Res. 205 (2): 320-5.
  7. Elsässer, H.P. et al. (1994) Growth of rat pancreatic acinar cells quantitated with a monoclonal antibody against the proliferating cell nuclear antigen.
    Cell Tissue Res. 276 (3): 603-9.
  8. Harris, L. et al. (2006) Characterisation of cell types in abalone (Haliotis spp.) tissues using immunohistochemical techniques
    Aquaculture 261: 1413-21
  9. Buggins, A.G. et al. (2001) Microenvironment produced by acute myeloid leukemia cells prevents T cell activation and proliferation by inhibition of NF-kappaB, c-Myc, and pRb pathways.
    J Immunol. 167: 6021-30.
  10. Kapitonova, M.Y. et al. (2010) Immunohistochemical characteristics of the hypophysis in normal conditions and chronic stress.
    Neurosci Behav Physiol. 40: 97-102.
  11. Franz-Odendaal, T.A. (2008) Toward understanding the development of scleral ossicles in the chicken, Gallus gallus.
    Dev Dyn. 237: 3240-51.
  12. Hashimoto, Y. et al. (2010) Rad51 protects nascent DNA from Mre11-dependent degradation and promotes continuous DNA synthesis.
    Nat Struct Mol Biol. 17: 1305-11.
  13. Fenton, M. et al. (2001) Cellular senescence after single and repeated balloon catheter denudations of rabbit carotid arteries.
    Arterioscler Thromb Vasc Biol. 21: 220-6.
  14. Park, J.H. et al. (2008) Gastric lesions and immune responses caused by long-term infection with Helicobacter heilmannii in C57BL/6 mice.
    J Comp Pathol. 139: 208-17.
  15. Izhak, L. et al. (2012) Dissecting the autocrine and paracrine roles of the CCR2-CCL2 axis in tumor survival and angiogenesis.
    PLoS One. 7: e28305.
  16. Di-poï, N. & Milinkovitch, M.C. (2016) The anatomical placode in reptile scale morphogenesis indicates shared ancestry among skin appendages in amniotes.
    Sci Adv. 2 (6): e1600708.
  17. Guzera, M. et al. (2016) In Vitro Influence of Mycophenolic Acid on Selected Parameters of Stimulated Peripheral Canine Lymphocytes.
    PLoS One. 11 (5): e0154429.
  18. Khlebnikov, V. V. et al. (2015) Developmental Characteristics of the Hypothalamo-Hypophyseal-Adrenal System in Chronic Heterotypical Stress
    Neuroscience and Behavioral Physiology. 46 (1): 100-5.
  19. Nakatsuka, M. & Kumabe, S (2018) Histological Alterations from Condyle Repositioning with Functional Appliances in Rats.
    J Clin Pediatr Dent. 42 (5): 391-7.

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