CD4 antibody | OX-38
Mouse anti Rat CD4 (Domain 1) antibody, clone OX-38 (MCA372G) used for the identification of CD4 expressing cells in rat spleen by flow cytometry.
Image caption:
FCM for proliferating Tregs induced by CD28SA. a Three-color FCM analysis of the splenic lymphocytes for lymphocyte markers, Treg transcription factor (Foxp3), and EdU at day 3 after CD28SA injection (0.5 mg/rat). b Dose response of Foxp3+Tregs with different markers to CD28SA. Absolute number of EdU+ proliferating Tregs with different lymphocyte markers/spleen, showing 0.5–1.0 mg induces an intense response
From: Kitazawa Y, Ueta H, Hünig T, Sawanobori Y, Matsuno K.
A novel multicolor immunostaining method using ethynyl deoxyuridine for analysis of in situ immunoproliferative response.
Histochem Cell Biol. 2015 Sep;144(3):195-208.
This image is from an open access article distributed under terms of a Creative Commons Attribution License.
Mouse anti Rat CD4 (Domain 1) antibody, clone OX-38 (MCA372G) used for the identification of CD4 expressing cells in rat spleen by flow cytometry and immunofluorescence.
Image caption:
Assessment of proliferating lymphocytes in the spleen activated by CD28SA. a FCM analysis of the splenic lymphocytes for lymphocyte markers and EdU at day 3 after CD28SA injection (0.5 mg/rat). Note increase in TCRαβ+, CD4+, or CD25+ EdU+ cells but not CD8β+EdU+ cells compared to control. b Dose response of splenic lymphocytes to CD28SA. Absolute number of EdU+ proliferating cells with different lymphocyte markers/spleen, showing 1.0 mg group induces the highest response. c Triple immunofluorescent staining for lymphocyte markers (indirect staining with Alexa-680-conjugated anti-mouse IgG, green), EdU (Alexa-594-conjugated azide, red) and type IV collagen (indirect staining with AMCA-conjugated anti-rabbit IgG, white). Day 3 after CD28SA injection (0.5 mg/rat). Pseudocolors were assigned using AxioVision software. Scale bar 100 μm. The splenic PALS area with the central arteries is depicted by type IV collagen. Proliferating cells with different markers are shown as green cells with red nuclei (inset of lower panel).
From: Kitazawa Y, Ueta H, Hünig T, Sawanobori Y, Matsuno K.
A novel multicolor immunostaining method using ethynyl deoxyuridine for analysis of in situ immunoproliferative response.
Histochem Cell Biol. 2015 Sep;144(3):195-208.
This image is from an open access article distributed under terms of a Creative Commons Attribution License.
Mouse anti Rat CD4 (Domain 1) antibody, clone OX-38 recognizes the rat CD4 cell surface antigen, also known as W3/25 antigen or T-cell surface antigen T4/Leu-3. CD4 is a 430 amino acid ~55 kDa single pass type I transmembrane glycoprotein expressed by T helper cells, monocytes and macrophages.
Clone OX-38 competes for binding with Mouse anti Rat CD4 antibody, clone W3/25 (MCA55R).
Mouse anti Rat CD4 (Domain 1) antibody, clone OX-38 has been utilized successfully for the in vitro depletion of T helper cells from slice cultures in studies looking at myelin loss in multiple sclerosis, the observed disruption being abrogated in these experimental conditions by T-cell depletion (Pusic et al. 2015).
Clone OX-38 competes for binding with Mouse anti Rat CD4 antibody, clone W3/25 (MCA55R).
Mouse anti Rat CD4 (Domain 1) antibody, clone OX-38 has been utilized successfully for the in vitro depletion of T helper cells from slice cultures in studies looking at myelin loss in multiple sclerosis, the observed disruption being abrogated in these experimental conditions by T-cell depletion (Pusic et al. 2015).
Product Details
- Target Species
- Rat
- Product Form
- Purified IgG - liquid
- Preparation
- Purified IgG prepared by affinity chromatography on Protein G
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
0.09% Sodium Azide - Carrier Free
- Yes
- Immunogen
- MLR generated rat T cells.
- Approx. Protein Concentrations
- IgG concentration 1.0 mg/ml
- Fusion Partners
- Spleen cells from immunised mice were fused with cells of the mouse NS1 myeloma cell line.
Storage Information
- Storage
- Store at +4oC or at -20oC if preferred.
This product should be stored undiluted.
Storage in frost-free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use. - Guarantee
- 12 months from date of despatch
More Information
- UniProt
- P05540
- Entrez Gene
- Cd4
- GO Terms
- GO:0007155 cell adhesion
- GO:0016021 integral to membrane
- GO:0006955 immune response
- GO:0019901 protein kinase binding
- GO:0045058 T cell selection
- Regulatory
- For research purposes only
Applications of CD4 antibody
| Application Name | Verified | Min Dilution | Max Dilution |
|---|---|---|---|
| Flow Cytometry | 1/100 | 1/200 | |
| Immunohistology - Frozen | |||
| Immunoprecipitation |
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
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Secondary Antibodies Available
Negative Isotype Controls Available
| Description | Product Code | Applications | Pack Size | List Price | Quantity |
|---|---|---|---|---|---|
| Mouse IgG2a Negative Control | MCA1210 | F | 100 Tests |
|
Product Specific References
References for CD4 antibody
-
Jefferies, W.A. et al. (1985) Authentic T helper CD4 (W3/25) antigen on rat peritoneal macrophages.
J Exp Med. 162 (1): 117-27. -
Barclay, A.N. (1981) The localization of populations of lymphocytes defined by monoclonal antibodies in rat lymphoid tissues.
Immunology. 42 (4): 593-600. -
Thomas, M.L. & Green, J.R. (1983) Molecular nature of the W3/25 and MRC OX-8 marker antigens for rat T lymphocytes: comparisons with mouse and human antigens.
Eur J Immunol. 13 (10): 855-8. -
Hall, B.M. et al. (1998) Anti-CD4 monoclonal antibody-induced tolerance to MHC-incompatible cardiac allografts maintained by CD4+ suppressor T cells that are not dependent upon IL-4.
J Immunol. 161 (10): 5147-56. -
Kitazawa, Y.et al. (2015) A novel multicolor immunostaining method using ethynyl deoxyuridine for analysis of in situ immunoproliferative response.
Histochem Cell Biol. 144 (3): 195-208. -
Pusic, A.D. et al. (2015) Spreading depression transiently disrupts myelin via interferon-gamma signaling.
Exp Neurol. 264: 43-54. -
Ueta, H. et al. (2018) Single blood transfusion induces the production of donor-specific alloantibodies and regulatory T cells mainly in the spleen.
Int Immunol. 30 (2): 53-67.
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