CD4 antibody | OX-38

Mouse anti Rat CD4 (Domain 1)

Product Type
Monoclonal Antibody
Clone
OX-38
Isotype
IgG2a
Specificity
CD4
Region
(DOMAIN 1)

Product Code Applications Pack Size List Price Your Price Qty
MCA372G
Datasheet Datasheet Datasheet
SDS Safety Datasheet SDS
C F IP 0.25 mg loader
List Price Your Price
loader
Search for Batch Specific Datasheets

Mouse anti Rat CD4 (Domain 1) antibody, clone OX-38 recognizes the rat CD4 cell surface antigen, also known as W3/25 antigen or T-cell surface antigen T4/Leu-3. CD4 is a 430 amino acid ~55 kDa single pass type I transmembrane glycoprotein expressed by T helper cells, monocytes and macrophages.

Clone OX-38 competes for binding with Mouse anti Rat CD4 antibody, clone W3/25 (MCA55R).

Mouse anti Rat CD4 (Domain 1) antibody, clone OX-38 has been utilized successfully for the in vitro depletion of T helper cells (Pusic et al. 2015).

Target Species
Rat
Product Form
Purified IgG - liquid
Preparation
Purified IgG prepared by affinity chromatography on Protein G
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.09%Sodium Azide
Carrier Free
Yes
Immunogen
MLR generated rat T cells.
Approx. Protein Concentrations
IgG concentration 1.0 mg/ml
Fusion Partners
Spleen cells from immunised mice were fused with cells of the mouse NS1 myeloma cell line.
Regulatory
For research purposes only
Guarantee
12 months from date of despatch

This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.

Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Flow Cytometry 1/100 1/200
Immunohistology - Frozen
Immunoprecipitation
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.

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References for CD4 antibody

  1. Jefferies, W.A. et al. (1985) Authentic T helper CD4 (W3/25) antigen on rat peritoneal macrophages.
    J Exp Med. 162 (1): 117-27.
  2. Barclay, A.N. (1981) The localization of populations of lymphocytes defined by monoclonal antibodies in rat lymphoid tissues.
    Immunology. 42 (4): 593-600.
  3. Thomas, M.L. & Green, J.R. (1983) Molecular nature of the W3/25 and MRC OX-8 marker antigens for rat T lymphocytes: comparisons with mouse and human antigens.
    Eur J Immunol. 13 (10): 855-8.
  4. Hall, B.M. et al. (1998) Anti-CD4 monoclonal antibody-induced tolerance to MHC-incompatible cardiac allografts maintained by CD4+ suppressor T cells that are not dependent upon IL-4.
    J Immunol. 161 (10): 5147-56.
  5. Kitazawa, Y.et al. (2015) A novel multicolor immunostaining method using ethynyl deoxyuridine for analysis of in situ immunoproliferative response.
    Histochem Cell Biol. 144 (3): 195-208.
  6. Pusic, A.D. et al. (2015) Spreading depression transiently disrupts myelin via interferon-gamma signaling.
    Exp Neurol. 264: 43-54.
  7. Ueta, H. et al. (2018) Single blood transfusion induces the production of donor-specific alloantibodies and regulatory T cells mainly in the spleen.
    Int Immunol. 30 (2): 53-67.

RRID
AB_322838
UniProt
P05540
Entrez Gene
Cd4
GO Terms
GO:0007155 cell adhesion
GO:0016021 integral to membrane
GO:0006955 immune response
GO:0019901 protein kinase binding
GO:0045058 T cell selection
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Please Note: All Products are "FOR RESEARCH PURPOSES ONLY"

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