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Notch 1 antibody | mN1A

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Anti Mouse Notch 1 Antibody, clone mN1A thumbnail image 1
Anti Mouse Notch 1 Antibody, clone mN1A gallery image 1
Figure A. Alexa Fluor 700 conjugated rat anti mouse CD3 (MCA500A700) and FITC conjugated mouse IgG1 isotype control (MCA928F) Figure B. Alexa Fluor 700 conjugated rat anti mouse CD3 (MCA500A700) and FITC conjugated mouse anti mouse NOTCH1 (MCA2410F). All experiments performed on mouse bone marrow. Data acquired on the ZE5 Cell Analyzer.

Mouse anti Mouse Notch 1

Product Type
Monoclonal Antibody
Clone
mN1A
Isotype
IgG1
Specificity
Notch 1

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Product Code Applications Pack Size List Price Your Price Qty
MCA2410GA
Datasheet Datasheet Datasheet
SDS Safety Datasheet SDS
C F * WB 0.1 mg loader
List Price Your Price
loader

Mouse anti Mouse Notch 1 antibody, clone mN1A recognizes Notch 1, one of the four major transmembrane receptors (Notch 1-4) of the Notch signaling pathway, which is activated through binding to DSL domain-containing membrane-bound specific ligands.

Notch 1 is expressed in a range of cells including haematopoietic cells in mouse foetal liver, adult thymus and bone marrow. Notch 1 signaling plays a role in follicular differentiation, tissue homeostasis, and in both CD4+ and CD8+ cell maturation in the thymus.

Target Species
Mouse
Species Cross-Reactivity
Target SpeciesCross Reactivity
Human
N.B. Antibody reactivity and working conditions may vary between species.
Product Form
Purified IgG - liquid
Preparation
Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.09% sodium azide (NaN3)
Carrier Free
Yes
Immunogen
Synthetic peptide corresponding to cdc10-NCR region within mouse Notch1.
Approx. Protein Concentrations
IgG concentration 1.0 mg/ml
Fusion Partners
Spleen cells from immunized BALB/c mice were fused with cells of the mouse SP2/0 myeloma cell line.
Regulatory
For research purposes only
Guarantee
12 months from date of despatch

This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.

Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Flow Cytometry 1
Immunohistology - Frozen 1/50 1/200
Western Blotting
  1. 1 Membrane permeabilization is required for this application. The use of Leucoperm (Product Code BUF09) is recommended for this purpose.
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
Flow Cytometry
Use 10μl of the suggested working dilution to label 1x106 cells in 100μl

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Description Goat anti Mouse IgG (H/L):Alk. Phos. (Multi Species Adsorbed)
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Description Goat anti Mouse IgG (H/L):DyLight®488 (Multi Species Adsorbed)
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Description Goat anti Mouse IgG (H/L):DyLight®550 (Multi Species Adsorbed)
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Description Goat anti Mouse IgG (H/L):DyLight®650 (Multi Species Adsorbed)
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Description Goat anti Mouse IgG (H/L):DyLight®680 (Multi Species Adsorbed)
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Description Goat anti Mouse IgG (H/L):DyLight®800 (Multi Species Adsorbed)
Goat anti Mouse IgG (H/L):FITC (Multi Species Adsorbed) STAR117F F 0.5 mg loader
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Description Goat anti Mouse IgG (H/L):FITC (Multi Species Adsorbed)
Goat anti Mouse IgG (H/L):HRP (Multi Species Adsorbed) STAR117P C E WB 0.5 mg loader
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Description Goat anti Mouse IgG (H/L):HRP (Multi Species Adsorbed)
Goat anti Mouse IgG (Fc):FITC STAR120F C F 1 mg loader
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Description Goat anti Mouse IgG (Fc):FITC
Goat anti Mouse IgG (Fc):HRP STAR120P E WB 1 mg loader
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Description Goat anti Mouse IgG (Fc):HRP
Rabbit F(ab')2 anti Mouse IgG:RPE STAR12A F 1 ml loader
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Description Rabbit F(ab')2 anti Mouse IgG:RPE
Rabbit F(ab')2 anti Mouse IgG:HRP (Human Adsorbed) STAR13B C E P RE WB 1 mg loader
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Description Rabbit F(ab')2 anti Mouse IgG:HRP (Human Adsorbed)
Goat anti Mouse IgG:FITC (Rat Adsorbed) STAR70 F 0.5 mg loader
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Description Goat anti Mouse IgG:FITC (Rat Adsorbed)
Goat anti Mouse IgG:RPE (Rat Adsorbed) STAR76 F 1 ml loader
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Description Goat anti Mouse IgG:RPE (Rat Adsorbed)
Goat anti Mouse IgG:HRP (Rat Adsorbed) STAR77 C E P 0.5 mg loader
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Description Goat anti Mouse IgG:HRP (Rat Adsorbed)
Goat anti Mouse IgG/A/M:HRP (Human Adsorbed) STAR87P E 1 mg loader
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Description Goat anti Mouse IgG/A/M:HRP (Human Adsorbed)
Rabbit F(ab')2 anti Mouse IgG:FITC STAR9B F 1 mg loader
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Description Rabbit F(ab')2 anti Mouse IgG:FITC

Source Reference

  1. Huppert, S.S. et al. (2000) Embryonic lethality in mice homozygous for a processing-deficient allele of Notch1.
    Nature. 405 (6789): 966-70.

References for Notch 1 antibody

  1. Ray, W.J. et al. (1999) Evidence for a physical interaction between presenilin and Notch.
    Proc Natl Acad Sci U S A. 96 (6): 3263-8.
  2. Espinosa, L. et al. (2002) p65-NFkappaB synergizes with Notch to activate transcription by triggering cytoplasmic translocation of the nuclear receptor corepressor N-CoR.
    J Cell Sci. 115 (Pt 6): 1295-303.
  3. Ren, M. and Cowell, J.K. (2011) Constitutive Notch pathway activation in murine ZMYM2-FGFR1-induced T-cell lymphomas associated with atypical myeloproliferative disease.
    Blood. 117: 6837-47.

Further Reading

  1. Kang-decker, N. et al. (2004) Loss of CBP causes T cell lymphomagenesis in synergy with p27Kip1 insufficiency.
    Cancer Cell. 5 (2): 177-89.

Western Blotting

RRID
AB_808683
UniProt
Q01705
Entrez Gene
Notch1
GO Terms
GO:0005515 protein binding
GO:0005509 calcium ion binding
GO:0001701 in utero embryonic development
GO:0001708 cell fate specification
GO:0001837 epithelial to mesenchymal transition
GO:0001889 liver development
GO:0002040 sprouting angiogenesis
GO:0002051 osteoblast fate commitment
GO:0003160 endocardium morphogenesis
GO:0003700 sequence-specific DNA binding transcription factor activity
GO:0004872 receptor activity
GO:0005887 integral to plasma membrane
GO:0005634 nucleus
GO:0007368 determination of left/right symmetry
GO:0007386 compartment pattern formation
GO:0007409 axonogenesis
GO:0007440 foregut morphogenesis
GO:0007492 endoderm development
GO:0008284 positive regulation of cell proliferation
GO:0014807 regulation of somitogenesis
GO:0021915 neural tube development
GO:0030216 keratinocyte differentiation
GO:0030324 lung development
GO:0030514 negative regulation of BMP signaling pathway
GO:0030900 forebrain development
GO:0031069 hair follicle morphogenesis
GO:0031490 chromatin DNA binding
GO:0032582 negative regulation of gene-specific transcription
GO:0035116 embryonic hindlimb morphogenesis
GO:0042640 anagen
GO:0043065 positive regulation of apoptosis
GO:0043066 negative regulation of apoptosis
GO:0043565 sequence-specific DNA binding
GO:0045618 positive regulation of keratinocyte differentiation
GO:0045668 negative regulation of osteoblast differentiation
GO:0046533 negative regulation of photoreceptor cell differentiation
GO:0048103 somatic stem cell division
GO:0048663 neuron fate commitment
GO:0048754 branching morphogenesis of a tube
GO:0060528 secretory columnal luminar epithelial cell differentiation involved in prostate glandular acinus development
GO:0060740 prostate gland epithelium morphogenesis
GO:0060768 regulation of epithelial cell proliferation involved in prostate gland development

MCA2410GA

1702

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