Notch 1 antibody | mN1A
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F ResetMouse anti Mouse Notch 1
- Product Type
- Monoclonal Antibody
- Clone
- mN1A
- Isotype
- IgG1
- Specificity
- Notch 1
Antibody Quality and Performance Guarantee
| Mouse anti Mouse Notch 1 antibody, clone mN1A recognizes Notch 1, one of the four major transmembrane receptors (Notch 1-4) of the Notch signaling pathway, which is activated through binding to DSL domain-containing membrane-bound specific ligands. Notch 1 is expressed in a range of cells including haematopoietic cells in mouse foetal liver, adult thymus and bone marrow. Notch 1 signaling plays a role in follicular differentiation, tissue homeostasis, and in both CD4+ and CD8+ cell maturation in the thymus. |
- Target Species
- Mouse
- Species Cross-Reactivity
-
Target Species Cross Reactivity Human - N.B. Antibody reactivity and working conditions may vary between species.
- Product Form
- Purified IgG - liquid
- Preparation
- Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
- 0.09% sodium azide (NaN3)
- Carrier Free
- Yes
- Immunogen
- Synthetic peptide corresponding to cdc10-NCR region within mouse Notch1.
- Approx. Protein Concentrations
- IgG concentration 1.0 mg/ml
- Fusion Partners
- Spleen cells from immunized BALB/c mice were fused with cells of the mouse SP2/0 myeloma cell line.
- Regulatory
- For research purposes only
- Guarantee
- 12 months from date of despatch
This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
| Application Name | Verified | Min Dilution | Max Dilution |
|---|---|---|---|
| Flow Cytometry 1 |  |
||
| Immunohistology - Frozen | |
1/50 | 1/200 |
| Western Blotting | |
- 1 Membrane permeabilization is required for this application. The use of Leucoperm (Product Code BUF09) is recommended for this purpose.
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
- Flow Cytometry
- Use 10μl of the suggested working dilution to label 1x106 cells in 100μl
Source Reference
-
Huppert, S.S. et al. (2000) Embryonic lethality in mice homozygous for a processing-deficient allele of Notch1.
Nature. 405 (6789): 966-70.
References for Notch 1 antibody
-
Ray, W.J. et al. (1999) Evidence for a physical interaction between presenilin and Notch.
Proc Natl Acad Sci U S A. 96 (6): 3263-8. -
Espinosa, L. et al. (2002) p65-NFkappaB synergizes with Notch to activate transcription by triggering cytoplasmic translocation of the nuclear receptor corepressor N-CoR.
J Cell Sci. 115 (Pt 6): 1295-303. -
Ren, M. and Cowell, J.K. (2011) Constitutive Notch pathway activation in murine ZMYM2-FGFR1-induced T-cell lymphomas associated with atypical myeloproliferative disease.
Blood. 117: 6837-47.
Further Reading
-
Kang-decker, N. et al. (2004) Loss of CBP causes T cell lymphomagenesis in synergy with p27Kip1 insufficiency.
Cancer Cell. 5 (2): 177-89.
- RRID
- AB_808683
- UniProt
- Q01705
- Entrez Gene
- Notch1
- GO Terms
- GO:0005515 protein binding
- GO:0005509 calcium ion binding
- GO:0001701 in utero embryonic development
- GO:0001708 cell fate specification
- GO:0001837 epithelial to mesenchymal transition
- GO:0001889 liver development
- GO:0002040 sprouting angiogenesis
- GO:0002051 osteoblast fate commitment
- GO:0003160 endocardium morphogenesis
- View More GO Terms
- GO:0003700 sequence-specific DNA binding transcription factor activity
- GO:0004872 receptor activity
- GO:0005887 integral to plasma membrane
- GO:0005634 nucleus
- GO:0007368 determination of left/right symmetry
- GO:0007386 compartment pattern formation
- GO:0007409 axonogenesis
- GO:0007440 foregut morphogenesis
- GO:0007492 endoderm development
- GO:0008284 positive regulation of cell proliferation
- GO:0014807 regulation of somitogenesis
- GO:0021915 neural tube development
- GO:0030216 keratinocyte differentiation
- GO:0030324 lung development
- GO:0030514 negative regulation of BMP signaling pathway
- GO:0030900 forebrain development
- GO:0031069 hair follicle morphogenesis
- GO:0031490 chromatin DNA binding
- GO:0032582 negative regulation of gene-specific transcription
- GO:0035116 embryonic hindlimb morphogenesis
- GO:0042640 anagen
- GO:0043065 positive regulation of apoptosis
- GO:0043066 negative regulation of apoptosis
- GO:0043565 sequence-specific DNA binding
- GO:0045618 positive regulation of keratinocyte differentiation
- GO:0045668 negative regulation of osteoblast differentiation
- GO:0046533 negative regulation of photoreceptor cell differentiation
- GO:0048103 somatic stem cell division
- GO:0048663 neuron fate commitment
- GO:0048754 branching morphogenesis of a tube
- GO:0060528 secretory columnal luminar epithelial cell differentiation involved in prostate glandular acinus development
- GO:0060740 prostate gland epithelium morphogenesis
- GO:0060768 regulation of epithelial cell proliferation involved in prostate gland development
MCA2410GA
1702
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