IgE antibody | E411 (5H2)
Mouse anti Human IgE
- Product Type
- Monoclonal Antibody
- Clone
- E411 (5H2)
- Isotype
- IgG2a
- Specificity
- IgE
Antibody Quality and Performance Guarantee
| Mouse anti Human IgE antibody, clone E411 (5H2) recognizes human IgE heavy chain, a 428 amino acid protein bearing four Ig-like domains. Mouse anti Human IgE antibody, clone E411 binds to an epitope expressed on Cε3 domain. Mouse anti Human IgE antibody, clone E411 has been successfully used as a capture reagent in the develpment of a sensitive Sandwich ELISA in combination with biotinylated Goat anti Human IgE (STAR147B) as a detection reagent. It hs also been used in the development of a bead based microfluidic assay for the measurement of patient IgE levels in combination with Mouse anti Human IgE antibody, clone E454 (0100-0414) indicating potential for the study and monitoring of IgE levels in allergic events (Proczek et al. 2012). |
- Target Species
- Human
- Product Form
- Purified IgG - liquid
- Preparation
- Purified IgG prepared by affinity chromatography on Protein G from ascites
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
- <0.1% Sodium Azide (NaN3)
- Immunogen
- Immunoglobulin E from human serum
- Approx. Protein Concentrations
- 1.0 mg/ml
- Regulatory
- For research purposes only
- Guarantee
- 12 months from date of despatch
This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
| Application Name | Verified | Min Dilution | Max Dilution |
|---|---|---|---|
| ELISA |  |
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| Flow Cytometry | |
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using the appropriate negative/positive controls.
| Description | Product Code | Applications | Pack Size | List Price | Your Price | Quantity | |
|---|---|---|---|---|---|---|---|
| Mouse IgG2a Negative Control | MCA929 | F | 100 Tests |
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| List Price | Your Price | ||||||
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| Description | Mouse IgG2a Negative Control | ||||||
References for IgE antibody
-
Wan, L. et al. (2010) Genetic variations in the CepsilonmX domain of human membrane-bound IgE.
Immunogenetics. 62: 273-80. -
Proczek, G. et al. (2012) Total serum IgE quantification by microfluidic ELISA using magnetic beads.
Anal Bioanal Chem. 402: 2645-53.
- RRID
- AB_617303
- UniProt
- P01854
- Entrez Gene
- IGHE
- GO Terms
- GO:0003823 antigen binding
- GO:0006955 immune response
0100-0413
121114
152919
If you cannot find the batch/lot you are looking for please contact our technical support team for assistance.
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Please Note: All Products are "FOR RESEARCH PURPOSES ONLY"
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