Mouse anti Human IgE antibody, clone E411 (5H2)
recognizes human IgE heavy chain, a 428 amino acid protein bearing four Ig-like domains. Mouse anti Human IgE antibody, clone E411 binds to an epitope expressed on Cε3 domain.
Mouse anti Human IgE antibody, clone E411 has been successfully used as a capture reagent in the develpment of a sensitive Sandwich ELISA in combination with biotinylated Goat anti Human IgE (STAR147B
) as a detection reagent. It hs also been used in the development of a bead based microfluidic assay for the measurement of patient IgE levels in combination with Mouse anti Human IgE antibody, clone E454 (0100-0414
) indicating potential for the study and monitoring of IgE levels in allergic events (Proczek et al. 2012
- Target Species
- Product Form
- Purified IgG - liquid
- Purified IgG prepared by affinity chromatography on Protein G from ascites
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
- Immunoglobulin E from human serum
- Approx. Protein Concentrations
- 1.0 mg/ml
- Store at +4oC or at -20oC if preferred.
Storage in frost-free freezers is not recommended.
This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
- 12 months from date of despatch
- Entrez Gene
- GO Terms
- For research purposes only
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Applications of IgE antibody
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using the appropriate negative/positive controls.
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Secondary Antibodies Available
Negative Isotype Controls Available