IgE antibody | E411 (5H2)

Mouse anti Human IgE

Product Type
Monoclonal Antibody
Clone
E411 (5H2)
Isotype
IgG2a
Specificity
IgE

Product Code Applications Pack Size List Price Your Price Qty
0100-0413
Datasheet Datasheet Datasheet
SDS Safety Datasheet SDS
E F 1 mg loader
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loader
0100-0413GA
Datasheet Datasheet Datasheet
SDS Safety Datasheet SDS
E F 0.2 mg loader
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loader
Search for Batch Specific Datasheets

Mouse anti Human IgE antibody, clone E411 (5H2) recognizes human IgE heavy chain, a 428 amino acid protein bearing four Ig-like domains. Mouse anti Human IgE antibody, clone E411 binds to an epitope expressed on Cε3 domain.

Mouse anti Human IgE antibody, clone E411 has been successfully used as a capture reagent in the develpment of a sensitive Sandwich ELISA in combination with biotinylated Goat anti Human IgE (STAR147B) as a detection reagent. It hs also been used in the development of a bead based microfluidic assay for the measurement of patient IgE levels in combination with Mouse anti Human IgE antibody, clone E454 (0100-0414) indicating potential for the study and monitoring of IgE levels in allergic events (Proczek et al. 2012).

Target Species
Human
Product Form
Purified IgG - liquid
Preparation
Purified IgG prepared by affinity chromatography on Protein G from ascites
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
<0.1% Sodium Azide (NaN3)
Immunogen
Immunoglobulin E from human serum
Approx. Protein Concentrations
1.0 mg/ml
Regulatory
For research purposes only
Guarantee
12 months from date of despatch

This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.

Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
ELISA
Flow Cytometry
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using the appropriate negative/positive controls.

Description Product Code Applications Pack Size List Price Your Price Quantity
Goat anti Mouse IgG:HRP (Rat Adsorbed) STAR77 C E P 0.5 mg loader
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loader
Description Goat anti Mouse IgG:HRP (Rat Adsorbed)
Goat anti Mouse IgG:FITC (Rat Adsorbed) STAR70 F 0.5 mg loader
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Description Goat anti Mouse IgG:FITC (Rat Adsorbed)
Rabbit F(ab')2 anti Mouse IgG:RPE STAR12A F 1 ml loader
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Description Rabbit F(ab')2 anti Mouse IgG:RPE
Goat anti Mouse IgG/A/M:Alk. Phos. STAR87A C E WB 1 mg loader
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loader
Description Goat anti Mouse IgG/A/M:Alk. Phos.
Rabbit F(ab')2 anti Mouse IgG:FITC STAR9B F 1 mg loader
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loader
Description Rabbit F(ab')2 anti Mouse IgG:FITC
Goat anti Mouse IgG:RPE (Rat Adsorbed) STAR76 F 1 ml loader
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loader
Description Goat anti Mouse IgG:RPE (Rat Adsorbed)
Goat anti Mouse IgG/A/M:HRP (Human Adsorbed) STAR87P E 1 mg loader
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loader
Description Goat anti Mouse IgG/A/M:HRP (Human Adsorbed)
Rabbit F(ab')2 anti Mouse IgG:HRP (Human Adsorbed) STAR13B C E P RE WB 1 mg loader
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loader
Description Rabbit F(ab')2 anti Mouse IgG:HRP (Human Adsorbed)
Goat anti Mouse IgG (Fc):FITC STAR120F C F 1 mg loader
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loader
Description Goat anti Mouse IgG (Fc):FITC
Goat anti Mouse IgG (Fc):HRP STAR120P E WB 1 mg loader
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loader
Description Goat anti Mouse IgG (Fc):HRP
Goat anti Mouse IgG (H/L):HRP (Multi Species Adsorbed) STAR117P C E WB 0.5 mg loader
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loader
Description Goat anti Mouse IgG (H/L):HRP (Multi Species Adsorbed)
Goat anti Mouse IgG (H/L):Alk. Phos. (Multi Species Adsorbed) STAR117A E WB 0.5 mg loader
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loader
Description Goat anti Mouse IgG (H/L):Alk. Phos. (Multi Species Adsorbed)
Goat anti Mouse IgG (H/L):FITC (Multi Species Adsorbed) STAR117F F 0.5 mg loader
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loader
Description Goat anti Mouse IgG (H/L):FITC (Multi Species Adsorbed)
Goat anti Mouse IgG (H/L):DyLight®488 (Multi Species Adsorbed) STAR117D488GA F IF 0.1 mg loader
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loader
Description Goat anti Mouse IgG (H/L):DyLight®488 (Multi Species Adsorbed)
Goat anti Mouse IgG (H/L):DyLight®680 (Multi Species Adsorbed) STAR117D680GA F WB 0.1 mg loader
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loader
Description Goat anti Mouse IgG (H/L):DyLight®680 (Multi Species Adsorbed)
Goat anti Mouse IgG (H/L):DyLight®800 (Multi Species Adsorbed) STAR117D800GA F IF WB 0.1 mg loader
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loader
Description Goat anti Mouse IgG (H/L):DyLight®800 (Multi Species Adsorbed)
Goat anti Mouse IgG (H/L):DyLight®650 (Multi Species Adsorbed) STAR117D650 F IF 0.1 mg loader
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loader
Description Goat anti Mouse IgG (H/L):DyLight®650 (Multi Species Adsorbed)
Goat anti Mouse IgG (H/L):DyLight®550 (Multi Species Adsorbed) STAR117D550 F IF WB 0.1 mg loader
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loader
Description Goat anti Mouse IgG (H/L):DyLight®550 (Multi Species Adsorbed)

Description Product Code Applications Pack Size List Price Your Price Quantity
Mouse IgG2a Negative Control MCA929 F 100 Tests loader
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loader
Description Mouse IgG2a Negative Control

References for IgE antibody

  1. Wan, L. et al. (2010) Genetic variations in the CepsilonmX domain of human membrane-bound IgE.
    Immunogenetics. 62: 273-80.
  2. Proczek, G. et al. (2012) Total serum IgE quantification by microfluidic ELISA using magnetic beads.
    Anal Bioanal Chem. 402: 2645-53.
  3. Brown, A.D. et al. (2012) IgE CH3 peptide vaccine
    US Patent Publication US 8298547 B2

RRID
AB_617303
UniProt
P01854
Entrez Gene
IGHE
GO Terms
GO:0003823 antigen binding
GO:0006955 immune response
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Please Note: All Products are "FOR RESEARCH PURPOSES ONLY"

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