IgE antibody | E411 (5H2)
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|Mouse anti Human IgE antibody, clone E411 (5H2) recognizes human IgE heavy chain, a 428 amino acid protein bearing four Ig-like domains. Mouse anti Human IgE antibody, clone E411 binds to an epitope expressed on Cε3 domain.
Mouse anti Human IgE antibody, clone E411 has been successfully used as a capture reagent in the develpment of a sensitive Sandwich ELISA in combination with biotinylated Goat anti Human IgE (STAR147B) as a detection reagent. It hs also been used in the development of a bead based microfluidic assay for the measurement of patient IgE levels in combination with Mouse anti Human IgE antibody, clone E454 (0100-0414) indicating potential for the study and monitoring of IgE levels in allergic events (Proczek et al. 2012).
- Target Species
- Product Form
- Purified IgG - liquid
- 0100-0413: Purified IgG prepared by affinity chromatography on Protein G from ascites
- 0100-0413GA: Purified IgG prepared by affinity chromatography on Protein G
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
- <0.1% Sodium Azide (NaN3)
- Immunoglobulin E from human serum
- Approx. Protein Concentrations
- 1.0 mg/ml
- For research purposes only
- 12 months from date of despatch
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.
|Application Name||Verified||Min Dilution||Max Dilution|
References for IgE antibody
Wan, L. et al. (2010) Genetic variations in the CepsilonmX domain of human membrane-bound IgE.
Immunogenetics. 62: 273-80.
Proczek, G. et al. (2012) Total serum IgE quantification by microfluidic ELISA using magnetic beads.
Anal Bioanal Chem. 402: 2645-53.
Brown, A.D. et al. (2012) IgE CH3 peptide vaccine
US Patent Publication US 8298547 B2
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