SDS-PAGE analysis of Recombinant Human UIgE kappa.The sample was analyzed under reducing, denaturing conditions (Lane 2) and non-reducing, denaturing conditions (Lane 3) and visualized using Coomassie staining.The heavy (HC) and light chains (LC) are indicated respectively
Sandwich ELISA analysis of human IgE using Mouse anti Human IgE antibody (0100-0413) as a capture reagent and biotinylated Goat anti Human IgE antibody (STAR147B) as a detection reagent with recombinant human IgE (HCA190) as antigen for generation of the standard curve. Detection is by HRP conjugated streptavidin and substrate. Microtitre plate is read at O.D. 450 nm on the Bio-Rad iMark Microplate Absorbance Reader (Bio-Rad Catalog 168-1135). Plasma and serum samples diluted 1:5 are indicated in green and orange respectively
Recombinant Human IgE kappa, clone 18705_hIgE is a recombinant human IgE antibody with a kappa light chain. Clone 18705_hIgE is specific for Green Fluorescent Protein and has no known reactivity with mammalian proteins or other antigens. This product is recommended for use as a standard in assays designed to measure IgE levels, or as a control antibody when using other human antibodies of the same isotype and subclass.
Clone 18705_hIgE forms part of a range of human recombinant immunoglobins containing the kappa light chain. A similar range of reagents containing the lambda light chain is also available from Bio-Rad.
Human IgE antibody selected from the HuCAL® phage display library and expressed in a human cell line. This antibody is supplied as a liquid.
Purified IgE prepared by affinity chromatography
Green Fluorescent Protein (GFP).
Approx. Protein Concentrations
Total protein concentration 0.5 mg/ml
Reagents In The Kit
Preparing The Antibody
Phosphate buffered saline
Store at +4oC or at -20oC if preferred. Storage in frost-free freezers is not recommended. This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
12 months from date of despatch
Sold under license of U.S. Patents 6,300,064, 6,696,248, 6,708,484, 6,753,136, European patent 0,859,841 and corresponding patents. This antibody was developed by Bio-Rad, Zeppelinstr. 4, 82178 Puchheim, Germany.
For in vitro research purposes only, unless otherwise specified in writing by Bio-Rad.
For research purposes only
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.