Human IgE | 18705_hIgE

//www.bio-rad-antibodies.com/human-ige-recombinant-protein-18705-hige-hca190.html?RenderPageSpecific=1
Human IgE | 18705_hIgE gallery image 1

Recombinant Human IgE Kappa (HCA190) used as a coating reagent in an indirect ELISA detected with Mouse anti Human IgE followed by Rabbit F(ab')2 anti Mouse IgG:HRP (STAR13B)

Enlarge
Human IgE | 18705_hIgE gallery image 2

SDS-PAGE analysis of Recombinant Human UIgE kappa.The sample was analyzed under reducing, denaturing conditions (Lane 2) and non-reducing, denaturing conditions (Lane 3) and visualized using Coomassie staining.The heavy (HC) and light chains (LC) are indicated respectively

Enlarge
Human IgE | 18705_hIgE gallery image 3

Sandwich ELISA analysis of human IgE using Mouse anti Human IgE antibody (0100-0413) as a capture reagent and biotinylated Goat anti Human IgE antibody (STAR147B) as a detection reagent with recombinant human IgE (HCA190) as antigen for generation of the standard curve. Detection is by HRP conjugated streptavidin and substrate. Microtitre plate is read at O.D. 450 nm on the Bio-Rad iMark Microplate Absorbance Reader (Bio-Rad Catalog 168-1135). Plasma and serum samples diluted 1:5 are indicated in green and orange respectively

Enlarge
  • Human IgE | 18705_hIgE thumbnail image 1
  • Human IgE | 18705_hIgE thumbnail image 2
  • Human IgE | 18705_hIgE thumbnail image 3
  • Recombinant Human IgE Kappa
(Rated 0.0 out of 5 based on 0 customer reviews)
  • Product Type
    Recombinant Protein
  • Clone
    18705_hIgE
  • Isotype
    IgE Kappa
1 Formats Available
    Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
    HCA190Edatasheet pdfdatasheet pdf0.1 mg
    HCA190
    Summary
    Secondary Antibodies
    Negative Isotype Controls
    Useful Reagents
    Positive Controls
    Histology Controls
    More Images
    References
    Reviews
    -
    • Recombinant Human IgE kappa, clone 18705_hIgE is a recombinant human IgE antibody with a kappa light chain. Clone 18705_hIgE is specific for Green Fluorescent Protein and has no known reactivity with mammalian proteins or other antigens. This product is recommended for use as a standard in assays designed to measure IgE levels, or as a control antibody when using other human antibodies of the same isotype and subclass.

      Clone 18705_hIgE forms part of a range of human recombinant immunoglobins containing the kappa light chain. A similar range of reagents containing the lambda light chain is also available from Bio-Rad.
    • Intended Use
    • Product Form
      Human IgE antibody selected from the HuCAL® phage display library and expressed in a human cell line. This antibody is supplied as a liquid.
    • Reconstitution
    • Preparation
      Purified IgE prepared by affinity chromatography
    • Preservative Stabilisers
      0.01% Thiomersal
    • Immunogen
      Green Fluorescent Protein (GFP).
    • Purity
    • Approx. Protein Concentrations
      Total protein concentration 0.5 mg/ml
    • Reagents In The Kit
    • Preparing The Antibody
    • Test Principle
    • Buffer Solution
      Phosphate buffered saline
    • Storage
      Store at +4oC or at -20oC if preferred.
      Storage in frost-free freezers is not recommended.
      This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
    • Shelf Life
      12 months from date of despatch
    • Acknowledgements
      Sold under license of U.S. Patents 6,300,064, 6,696,248, 6,708,484, 6,753,136, European patent 0,859,841 and corresponding patents. This antibody was developed by Bio-Rad, Zeppelinstr. 4, 82178 Puchheim, Germany, a wholly owned subsidiary of Bio-Rad Laboratories Inc.
    • Licensed Use
      For in vitro research purposes only, unless otherwise specified in writing by Bio-Rad.
    • Regulatory
      For research purposes only
    • This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.

    • Application NameYesNoMin DilutionMax Dilution
      ELISA

    • Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
    • Technical Advice
      Recommended protocols and further information about HuCAL recombinant antibody technology can be found in the HuCAL Antibodies Technical Manual
    • Recommended Protocol
    • ELISA
    • Immunohistology
    • Histology Positive Control Tissue
    • Immunofluorescence
    • Western Blotting
    • Instructions For Use

    Additional Human IgE Formats

    Formats Clone Applications Sizes available
    Human IgE : Purified 18705_hIgE E 0.1 mg
    • Copyright © 2016 Bio-Rad

    Recommended Secondary Antibody

      Recommended Negative Isotype Control

        Useful Reagents

          DescriptionProduct CodePack SizeApplicationsList PriceQuantity
          Recombinant Human IgE LambdaHCA1710.1 mgE
          HCA171

          Recommended Positive Controls

            Histology Controls

              Write your review

              You may also be interested in...