CD2 antibody | LT2

Mouse anti Human CD2

Product Type
Monoclonal Antibody
Clone
LT2
Isotype
IgG2b
Specificity
CD2

Product Code Applications Pack Size List Price Your Price Qty
MCA1194GA
Datasheet Datasheet Datasheet
SDS Safety Datasheet SDS
C * F IP 0.1 mg loader
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loader
MCA1194
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SDS Safety Datasheet SDS
C * F IP 0.2 mg loader
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loader
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Mouse anti Human CD2 antibody, clone LT2 recognizes human CD2, a single chain transmembrane glycoprotein of 50 kDa, expressed by T cells, NK cells and thymocytes.

CD2 is a receptor for CD58 (LFA-3), and is important in mediating the interaction between T cells and target cells or antigen presenting cells.

Mouse anti Human CD2 antibody, clone LT2 inhibits NK cell mediated cytotoxicity, and ConA and PHA mediated blast transformation.

Target Species
Human
Species Cross-Reactivity
Target SpeciesCross Reactivity
Rhesus Monkey
Chimpanzee
Cynomolgus monkey
N.B. Antibody reactivity and working conditions may vary between species.
Product Form
Purified IgG - liquid.
Preparation
Purified IgG prepared by affinity chromatography on Protein A from ascites
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.09%Sodium Azide
Immunogen
Normal human peripheral blood lymphocytes.
Approx. Protein Concentrations
IgG concentration 1 mg/ml
Fusion Partners
Spleen cells from immunised BALB/c mice were fused with cells of the mouse X63.653 myeloma cell line.
Regulatory
For research purposes only
Guarantee
12 months from date of despatch

This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.

Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Flow Cytometry 1/50 1/100
Immunohistology - Frozen 1 1/200 1/400
Immunohistology - Paraffin
Immunoprecipitation
  1. 1The epitope recognised by this antibody is reported to be sensitive to formaldehyde fixation and tissue processing. Bio-Rad recommends the use of acetone fixation for frozen sections.
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
Flow Cytometry
Use 10ul of the suggested working dilution to label 106 cells in 100ul.
Histology Positive Control Tissue
Human Tonsil

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Description Goat anti Mouse IgG (H/L):DyLight®650 (Multi Species Adsorbed)
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Description Mouse IgG2b Negative Control

References for CD2 antibody

  1. Moingeon, P. et al. (1989) The structural biology of CD2.
    Immunol Rev. 111: 111-44.
  2. Beyers, A.D. et al. (1989) Activation of T lymphocytes via monoclonal antibodies against rat cell surface antigens with particular reference to CD2 antigen.
    Immunol Rev. 111: 59-77.
  3. Lin, C.W. et al. (2005) CD94 1A transcripts characterize lymphoblastic lymphoma/leukemia of immature natural killer cell origin with distinct clinical features.
    Blood. 106 (10): 3567-74.
  4. Kap, Y.S. et al. (2009) A monoclonal antibody selection for immunohistochemical examination of lymphoid tissues from non-human primates.
    J Histochem Cytochem. 57: 1159-67.
  5. Bruger, A.M. et al. (2020) Protocol to assess the suppression of T-cell proliferation by human MDSC.
    Methods Enzymol. 632: 155-92.

Synonyms
LFA-2
RRID
AB_321230
UniProt
P06729
Entrez Gene
CD2
GO Terms
GO:0001766 membrane raft polarization
GO:0007596 blood coagulation
GO:0004872 receptor activity
GO:0005887 integral to plasma membrane
GO:0006917 induction of apoptosis
GO:0007166 cell surface receptor linked signaling pathway
GO:0016337 cell-cell adhesion
GO:0030101 natural killer cell activation
GO:0030887 positive regulation of myeloid dendritic cell activation
GO:0042110 T cell activation
GO:0045580 regulation of T cell differentiation
GO:0050900 leukocyte migration
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Please Note: All Products are "FOR RESEARCH PURPOSES ONLY"

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