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CD4 antibody | RPA-T4

Mouse anti Human CD4:StarBright Blue 700

Product Type
Monoclonal Antibody
Clone
RPA-T4
Isotype
IgG1
Specificity
CD4

Product Code Applications Pack Size List Price Your Price Qty
MCA1267SBB700T
Datasheet Datasheet Datasheet
SDS Safety Datasheet SDS
F 25 Tests/0.125ml loader
List Price Your Price
loader
MCA1267SBB700
Datasheet Datasheet Datasheet
SDS Safety Datasheet SDS
F 100 Tests/0.5ml loader
List Price Your Price
loader

Mouse anti human CD4 antibody, clone RPA-T4 recognizes human CD4, a ~55 kDa cell surface glycoprotein, primarily expressed on a subpopulation of T lymphocytes, on peripheral blood monocytes and on tissue macrophages. Epitope mapping shows that antibodies, produced by clone RPA-T4, recognize an epitope within domain 1 of the extracellular region of the CD4 molecule.

Mouse anti human CD4 antibody, clone RPA-T4 blocks gp120-CD4 interaction and inhibits syncytium formation (Piatier-Tonneau et al, 1997). The use of Mouse anti Human CD4:Low Endotoxin (MCA1267EL) is recommended for functional assays.

Target Species
Human
Product Form
Purified IgG conjugated to StarBright Blue 700 - liquid
Preparation
Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.09% Sodium Azide (NaN3)
1% Bovine Serum Albumin
0.1% Pluronic F68
0.1% PEG 3350
0.05% Tween 20
Immunogen
Human PHA blasts
Fusion Partners
Spleen cells from immunized BALB/c mice were fused with cells of the mouse NSI myeloma cell line
Max Ex/Em
Fluorophore Excitation Max (nm) Emission Max (nm)
StarBright Blue 700 473 703
Regulatory
For research purposes only
Guarantee
12 months from date of despatch
Acknowledgements
This product is covered by U.S. Patent No. 10,150,841 and related U.S. and foreign counterparts

Store at +4°C. DO NOT FREEZE.
This product should be stored undiluted.

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Flow Cytometry Neat
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
Flow Cytometry
Use 5μl of the suggested working dilution to label 106 cells in 100μl. Best practices suggest a 5 minutes centrifugation at 6,000g prior to sample application.

How to Use the Spectraviewer

Watch the Tool Tutorial Video ▸
  • Start by selecting the application you are interested in, with the option to select an instrument from the drop down menu or create a customized instrument
  • Select the fluorophores or fluorescent proteins you want to include in your panel to check compatibility
  • Select the lasers and filters you wish to include
  • Select combined or multi-laser view to visualize the spectra

Description Product Code Applications Pack Size List Price Your Price Quantity
Human Seroblock BUF070A F 50 Test
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Description Human Seroblock
Human Seroblock BUF070B F 200 Test
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Description Human Seroblock

References for CD4 antibody

  1. Piatier-Tonneau, D. (1997) CD4 workshop panel report.
    In: Leucocyte Typing VI: White Cell Differentiation Antigens: Proceedings of the Sixth International Workshop and Conference Held in Kobe, Japan, 10-14 November 1996. Garland Pub., 1998.
  2. Zarkesh-Esfahani, H. et al. (2001) High-dose leptin activates human leukocytes via receptor expression on monocytes.
    J Immunol. 167 (8): 4593-9.
  3. Wright, G.J. et al. (2001) The unusual distribution of the neuronal/lymphoid cell surface CD200 (OX2) glycoprotein is conserved in humans.
    Immunology. 102 (2): 173-9.
  4. Pentón-Rol, G. et al. (2011) C-Phycocyanin ameliorates experimental autoimmune encephalomyelitis and induces regulatory T cells.
    Int Immunopharmacol. 11 (1): 29-38.
  5. Zhang, Y. et al. (2013) Accelerated in vivo. proliferation of memory phenotype CD4+ T-cells in human HIV-1 infection irrespective of viral chemokine co-receptor tropism.
    PLoS Pathog. 9 (4): e1003310.
  6. Bughani, U. et al. (2017) T cell activation and differentiation is modulated by a CD6 domain 1 antibody Itolizumab.
    PLoS One. 12 (7): e0180088.
  7. Agrawal, S.M. et al. (2013) Extracellular matrix metalloproteinase inducer shows active perivascular cuffs in multiple sclerosis.
    Brain. 136 (Pt 6): 1760-77.
  8. Malmassari, S.L. et al. (2007) Impact of hepatitis B virus basic core promoter mutations on T cell response to an immunodominant HBx-derived epitope.
    Hepatology. 45 (5): 1199-209.
  9. View The Latest Product References
  10. Wooldridge, L. et al. (2006) Anti-coreceptor antibodies profoundly affect staining with peptide-MHC class I and class II tetramers.
    Eur J Immunol. 36 (7): 1847-55.
  11. Wildum, S. et al. (2006) Contribution of Vpu, Env, and Nef to CD4 down-modulation and resistance of human immunodeficiency virus type 1-infected T cells to superinfection.
    J Virol. 80 (16): 8047-59.
  12. Kirchhof, J. et al. (2018) Learned immunosuppressive placebo responses in renal transplant patients.
    Proc Natl Acad Sci U S A. 115 (16): 4223-7.
  13. Kelleher, M. et al. (2011) Comparative Kinetics of Immune Responses and Changes in Cellular Sub-Sets Detected in Colorectal Cancer Patients Vaccinated with MVA-5T4 (TroVax) Administered Alongside Two Different Chemotherapy Regimens
    J Cancer Therapy. 02 (01): 54-64.

Flow Cytometry

UniProt
P01730
Entrez Gene
CD4
GO Terms
GO:0001948 glycoprotein binding
GO:0007155 cell adhesion
GO:0016021 integral to membrane
GO:0004888 transmembrane receptor activity
GO:0007169 transmembrane receptor protein tyrosine kinase signaling pathway
GO:0005201 extracellular matrix structural constituent
GO:0005769 early endosome
GO:0005789 endoplasmic reticulum membrane
GO:0006948 induction by virus of host cell-cell fusion
GO:0006955 immune response
GO:0008270 zinc ion binding
GO:0015026 coreceptor activity
GO:0019059 initiation of viral infection
GO:0019901 protein kinase binding
GO:0030260 entry into host cell
GO:0031295 T cell costimulation
GO:0032507 maintenance of protein location in cell
GO:0042101 T cell receptor complex
GO:0042289 MHC class II protein binding
GO:0042803 protein homodimerization activity
GO:0045058 T cell selection
GO:0045086 positive regulation of interleukin-2 biosynthetic process
GO:0045234 protein palmitoleylation
GO:0045860 positive regulation of protein kinase activity
GO:0050690 regulation of defense response to virus by virus
GO:0050852 T cell receptor signaling pathway

MCA1267SBB700T

100007474 64596751

MCA1267SBB700

100004737 100006326 100007739

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