CD19 antibody | LE-CD19

Mouse anti Human CD19

Product Type
Monoclonal Antibody
Clone
LE-CD19
Isotype
IgG1
Format
Purified
Specificity
CD19

Product Code Applications Pack Size List Price Your Price Qty
MCA2454T
Datasheet Datasheet Datasheet
SDS Safety Datasheet SDS
E F * P * 20 µg loader
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loader
MCA2454
Datasheet Datasheet Datasheet
SDS Safety Datasheet SDS
E F * IP P * WB 0.2 mg loader
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loader
Search for Batch Specific Datasheets

Mouse anti Human CD19 antibody, clone LE-CD19 recognizes an epitope within the C-terminal cytoplasmic tail sequence of human CD19, a single pass type I transmembrane glycoprotein containing two C2 type Ig-like domains in the N-terminal extracellular region and four potential phosphorylation sites for tyrosine together with a single serine in the cytoplasmic region.

Human CD19 is expressed on virtually all cells of the B-cell lineage with the exception of plasma cells and plays a regulatory role in B-cell differentiation and proliferation.

B-cells are essential for antibody production and mutations in the CD19 gene can lead to an immunodeficiency syndrome, CIVD3 characterized by hypogammaglobulinemia leading to recurrent infections and the inability to mount an antibody mediated response to immune insult. Although immunoglobulin production is impaired B-cell precursors appear in normal numbers together with some reduction in more mature B-cell forms (van Zelm et al. 2006). B-cells have also been implicated in the progression and pathogenesis of multiple sclerosis and are common components of both active and chronic MS lesions and well as the CSF (Ritchie et al. 2004)

Mouse anti Human CD19 antibody, clone LE-CD19 has been successfully employed for the immunohistochemical demonstration of CD19 in formalin fixed, paraffin embedded tissues (Streeck, H. et al. 2011) and for the detection of CD19 in cell lysates by Western blotting.
PrecisionAb Antibodies

Target Species
Human
Product Form
Purified IgG - liquid
Preparation
Purified IgG prepared by affinity chromatography on Protein A from tissue culture supernatant
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.09%Sodium Azide
Carrier Free
Yes
Immunogen
CD19 peptide CGPDPAWGGGGRMGTWSTR (C-terminus) coupled to KLH.
Approx. Protein Concentrations
IgG concentration 1.0 mg/ml
Fusion Partners
Spleen cells from immunised BALB/c mice were fused with cells of the X63-Ag8.653 myeloma cell line.
Regulatory
For research purposes only
Guarantee
12 months from date of despatch
Acknowledgements
PrecisionAb is a trademark of Bio-Rad Laboratories

This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.

Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
ELISA
Flow Cytometry 1 1/100 1/200
Immunohistology - Paraffin 2 1/100 1/200
Immunoprecipitation
Western Blotting Reducing conditions
  1. 1Membrane permeabilization is required for this application. The use of Leucoperm (Product Code BUF09) is recommended for this purpose.
  2. 2This product requires antigen retrieval using heat treatment prior to staining of paraffin sections.Sodium citrate buffer pH 6.0 is recommended for this purpose.

The PrecisionAb label is reserved for antibodies that meet the defined performance criteria within Bio-Rad's ongoing antibody validation programme. Click here to learn how we validate our PrecisionAb range. Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
Flow Cytometry
Use 10ul of the suggested working dilution to label 1x106 cells in 100ul.
Histology Positive Control Tissue
Tonsil

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Description Goat anti Mouse IgG (Fc):FITC
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References for CD19 antibody

  1. Ogembo, J.G. et al. (2012) SIRPα/CD172a and FHOD1 Are Unique Markers of Littoral Cells, a Recently Evolved Major Cell Population of Red Pulp of Human Spleen.
    J Immunol. 188: 4496-505.
  2. Streeck, H. et al. (2011) Epithelial adhesion molecules can inhibit HIV-1-specific CD8⁺ T-cell functions
    Blood. 117: 5112-22.
  3. Herting, F. et al. (2014) Enhanced anti-tumor activity of the glycoengineered type II CD20 antibody obinutuzumab (GA101) in combination with chemotherapy in xenograft models of human lymphoma.
    Leuk Lymphoma. 55 (9): 2151-60.
  4. Inoue, T. et al. (2010) Differential expression of glycogenes in tonsillar B lymphocytes in association with proteinuria and renal dysfunction in IgA nephropathy.
    Clin Immunol. 136 (3): 447-55.
  5. Yang, C. et al. (2013) Prognostic significance of B-cells and pSTAT3 in patients with ovarian cancer.
    PLoS One. 8 (1): e54029.
  6. Nichele, I. et al. (2012) VR09 cell line: an EBV-positive lymphoblastoid cell line with in vivo. characteristics of diffuse large B cell lymphoma of activated B-cell type.
    PLoS One. 7 (12): e52811.
  7. Heylmann, D. et al. (2021) Comparison of DNA repair and radiosensitivity of different blood cell populations.
    Sci Rep. 11 (1): 2478.

RRID
AB_566586
UniProt
P15391
Entrez Gene
CD19
GO Terms
GO:0005515 protein binding
GO:0005057 receptor signaling protein activity
GO:0005887 integral to plasma membrane
GO:0006968 cellular defense response
View more products with CD19 specificity

Please Note: All Products are "FOR RESEARCH PURPOSES ONLY"

View all Anti-Human Products