CD152 antibody | BNI3

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Mouse anti Human CD152

Mouse anti Human CD152:RPE

Product Type
Monoclonal Antibody
Clone
BNI3
Isotype
IgG2a
Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
MCA1724 C* F* IF IP 0.2 mg
MCA1724PET F* 25 Tests
MCA1724PE F* 100 Tests/2ml
Mouse anti Human CD152 antibody, clone BNI3 recognizes human CD152, also known as CTLA-4 (cytotoxic T-lymphocyte-associated antigen 4), an inhibitory receptor and negative regulator of T-cell responses. CD152 is a single pass type 1 transmembrane protein belonging to the immunoglobulin superfamily containing a single Ig-v-like domain in the extracellular region.

CD152 along with CD28 binds to the co-stimulatory molecules CD80 and CD86 (Azuma et al. 1993).

Mouse anti human CD152 antibody, clone BNI3 is able to block ligand binding on the Raji B-cell line (Steiner et al. 2001) and blocks binding of an alternative clone, BNI8 to CTLA-4/Ig in ELISA. Mouse anti Human CD152 antibody, clone BNI3 binds to the same epitope as classified anti CTLA-4 clones 11D4 and 10A8 (Wang et al. In: Leukocyte typing VI 1997 Garland Publishing Inc. pp97-98, Bull World Health Organ. 1997).

The cytoplasmic domain of CD152 contains a critical tyrosine at residue 201 phosphorylated by Janus Kinase 2 which subsequently controls surface expression through regulation of CD152 interaction with AP-2 (Shiratori et al. 1997, Chikuma et al. 2000). CD152 is expressed primarily as an intracellular antigen with transport to the cell surface under tight regulation of several molecules including Trim, PLD and TIRC7, CD152 also demonstrates rapid internalization once expressed at the cell surface (Valk et al. 2008). CD152 plays a significant role in maintaining tolerance to self antigens and defects in CD152 presentation and expression has been implicated in a number of autoimmune diseases (Romo-Tena et al. 2013).

Product Details

Target Species
Human
Product Form
Purified IgG - liquid
Product Form
Purified IgG conjugated to R. Phycoerythrin (RPE) - liquid
Preparation
Purified by affinity chromatography from ascites
Preparation
Purified IgG prepared by affinity chromatography on Protein A from ascites
Buffer Solution
Phosphate buffered saline
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.09%Sodium Azide
0.5%Bovine Serum Albumin
Preservative Stabilisers
Pack Size: 25 Tests
0.09%Sodium Azide
0.2%Bovine Serum Albumin
Pack Size: 100 Tests/2ml
0.1% Sodium Azide (NaN3)
2% Bovine Serum Albumin
Immunogen
Human CTLA-4/human IgG heavy chain fusion protein.
Approx. Protein Concentrations
IgG concentration 1.0 mg/ml
Fusion Partners
Spleen cells from immunised BALB/c mice were fused with cells of the mouse P3/X63-Ag8.653 myeloma cell line.

Storage Information

Storage
Store at +4oC or at -20oC if preferred.

This product should be stored undiluted.

Storage in frost free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Storage
Store at +4oC.

DO NOT FREEZE

This product should be stored undiluted. This product is photosensitive and should be protected from light. Should this product contain a precipitate we recommend microcentrifugation before use.
Shelf Life
18 months from date of despatch.
Shelf Life
Please see label for expiry date.

More Information

UniProt
P16410 Related reagents
Entrez Gene
CTLA4 Related reagents
GO Terms
GO:0005887 integral to plasma membrane
GO:0005794 Golgi apparatus
GO:0006955 immune response
GO:0006974 response to DNA damage stimulus
GO:0009897 external side of plasma membrane
GO:0030889 negative regulation of B cell proliferation
GO:0031295 T cell costimulation
GO:0043065 positive regulation of apoptosis
GO:0045334 clathrin-coated endocytic vesicle
GO:0045590 negative regulation of regulatory T cell differentiation
GO:0048471 perinuclear region of cytoplasm
GO:0050853 B cell receptor signaling pathway
Regulatory
For research purposes only

Applications of CD152 antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Flow Cytometry 1 Neat 1/10
Immunofluorescence
Immunohistology - Frozen 2 5ug/ml 50ug/ml
Immunohistology - Paraffin
Immunoprecipitation
Flow Cytometry 1 Neat
  1. 1Membrane permeabilization is required for this application. Bio-Rad recommend the use of Leucoperm (Product Code BUF09) for this purpose.
  2. 2The epitope recognised by this antibody is reported to be sensitive to formaldehyde fixation and tissue processing. Bio-Rad recommends the use of acetone fixation for frozen sections.
  1. 1 Membrane permeabilisation is required for this application. Bio-Rad recommends the use of Leucoperm (Product Code BUF09) for this purpose.
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.

Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
Flow Cytometry
Use 10ul of the suggested working dilution to label 1x106 cells in 100ul.
Flow Cytometry
Use 20ul of the suggested working dilution to label 5x105 cells in 100ul.

Secondary Antibodies Available

Description Product Code Pack Size Applications List Price Quantity
Human anti Mouse IgG2a:FITC HCA037F 0.1 mg F
Human anti Mouse IgG2a:HRP HCA037P 0.1 mg E
Goat anti Mouse IgG (H/L):Alk. Phos. (Multi Species Adsorbed) STAR117A 0.5 mg E WB
Goat anti Mouse IgG (H/L):DyLight®488 (Multi Species Adsorbed) STAR117D488GA 0.1 mg F IF
Goat anti Mouse IgG (H/L):DyLight®549 (Multi Species Adsorbed) STAR117D549GA 0.1 mg F IF WB
Goat anti Mouse IgG (H/L):DyLight®649 (Multi Species Adsorbed) STAR117D649GA 0.1 mg F IF
Goat anti Mouse IgG (H/L):DyLight®680 (Multi Species Adsorbed) STAR117D680GA 0.1 mg F WB
Goat anti Mouse IgG (H/L):DyLight®800 (Multi Species Adsorbed) STAR117D800GA 0.1 mg F IF WB
Goat anti Mouse IgG (H/L):FITC (Multi Species Adsorbed) STAR117F 0.5 mg F
Goat anti Mouse IgG (H/L):HRP (Multi Species Adsorbed) STAR117P 0.5 mg E WB
Goat anti Mouse IgG (Fc):FITC STAR120F 1 mg C F
Goat anti Mouse IgG (Fc):HRP STAR120P 1 mg E WB
Rabbit F(ab')2 anti Mouse IgG:RPE STAR12A 1 ml F
Rabbit F(ab')2 anti Mouse IgG:HRP (Human Adsorbed) STAR13B 1 mg C E P RE WB
Goat anti Mouse IgG:FITC (Rat Adsorbed) STAR70 0.5 mg F
Goat anti Mouse IgG:RPE (Rat Adsorbed) STAR76 1 ml F
Goat anti Mouse IgG:HRP (Rat Adsorbed) STAR77 0.5 mg C E P
Goat anti Mouse IgG/A/M:Alk. Phos. STAR87A 1 mg C E WB
Goat anti Mouse IgG/A/M:HRP (Human Adsorbed) STAR87P 1 mg E
Rabbit F(ab')2 anti Mouse IgG:Dylight®800 STAR8D800GA 0.1 mg F IF WB
Rabbit F(ab')2 anti Mouse IgG:FITC STAR9B 1 mg F

Negative Isotype Controls Available

Description Product Code Pack Size Applications List Price Quantity
Mouse IgG2a Negative Control MCA929 100 Tests F
Mouse IgG2a Negative Control:RPE MCA929PE 100 Tests F

Useful Reagents Available

Description Product Code Pack Size Applications List Price Quantity
Human Seroblock BUF070A 50 Test F
Human Seroblock BUF070B 200 Test F

Application Based External Images

Immunocytochemistry

Immunofluorescence

Product Specific References

References for CD152 antibody

  1. Linsley, P.S. et al. (1992) Coexpression and functional cooperation of CTLA-4 and CD28 on activated T lymphocytes.
    J Exp Med. 176 (6): 1595-604.
  2. Kuiper, H.M. et al. (1995) Activated T cells can induce high levels of CTLA-4 expression on B cells.
    J Immunol. 155 (4): 1776-83.
  3. Castan, J. et al. (1997) Accumulation of CTLA-4 expressing T lymphocytes in the germinal centres of human lymphoid tissues.
    Immunology. 90 (2): 265-71.
  4. Lee, C.C. et al. (2009) The regulatory function of umbilical cord blood CD4(+) CD25(+) T cells stimulated with anti-CD3/anti-CD28 and exogenous interleukin (IL)-2 or IL-15.
    Pediatr Allergy Immunol. 20 (7): 624-32.
  5. Pistillo, M.P. et al. (2003) CTLA-4 is not restricted to the lymphoid cell lineage and can function as a target molecule for apoptosis induction of leukemic cells.
    Blood. 101: 202-9.
  6. Tan, P.H. et al. (2005) Creation of tolerogenic human dendritic cells via intracellular CTLA4: a novel strategy with potential in clinical immunosuppression.
    Blood. 106: 2936-43.
  7. Steiner, K. et al. (2001) Increased expression of CTLA-4 (CD152) by T and B lymphocytes in Wegener's granulomatosis.
    Clin Exp Immunol. 126: 143-50.
  8. Lu, C.H. et al. (2016) DNA Methyltransferase Inhibitor Promotes Human CD4+CD25hFOXP3+ Regulatory T Lymphocyte Induction under Suboptimal TCR Stimulation.
    Front Immunol. 7: 488.
  9. Steiner, K. et al. (1999) Enhanced expression of CTLA-4 (CD152) on CD4+ T cells in HIV infection.
    Clin Exp Immunol. 115 (3): 451-7.
  10. Ward, F.J. et al. (2013) The soluble isoform of CTLA-4 as a regulator of T-cell responses.
    Eur J Immunol. 43 (5): 1274-85.

Further Reading

  1. Chin, L.T. et al. (2008) Immune intervention with monoclonal antibodies targeting CD152 (CTLA-4) for autoimmune and malignant diseases.
    Chang Gung Med J. 31: 1-15.