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CD152 antibody | BNI3

Product Code Applications Pack Size List Price Your Price Qty
MCA1724GA
Datasheet Datasheet Datasheet
SDS Safety Datasheet SDS
C * E F * IF IP 0.1 mg loader
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loader
MCA1724
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SDS Safety Datasheet SDS
C * E F * IF IP 0.2 mg loader
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loader

Mouse anti Human CD152 antibody, clone BNI3 recognizes human CD152, also known as CTLA-4 (cytotoxic T-lymphocyte-associated antigen 4), an inhibitory receptor and negative regulator of T-cell responses. CD152 is a single pass type 1 transmembrane protein belonging to the immunoglobulin superfamily containing a single Ig-v-like domain in the extracellular region.

CD152 along with CD28 binds to the co-stimulatory molecules CD80 and CD86 (Azuma et al. 1993).

Mouse anti human CD152 antibody, clone BNI3 is able to block ligand binding on the Raji B-cell line (Steiner et al. 2001) and blocks binding of an alternative clone, BNI8 to CTLA-4/Ig in ELISA. Mouse anti Human CD152 antibody, clone BNI3 binds to the same epitope as classified anti CTLA-4 clones 11D4 and 10A8 (Wang et al. In: Leukocyte typing VI 1997 Garland Publishing Inc. pp97-98, Bull World Health Organ. 1997).

The cytoplasmic domain of CD152 contains a critical tyrosine at residue 201 phosphorylated by Janus Kinase 2 which subsequently controls surface expression through regulation of CD152 interaction with AP-2 (Shiratori et al. 1997, Chikuma et al. 2000). CD152 is expressed primarily as an intracellular antigen with transport to the cell surface under tight regulation of several molecules including Trim, PLD and TIRC7, CD152 also demonstrates rapid internalization once expressed at the cell surface (Valk et al. 2008). CD152 plays a significant role in maintaining tolerance to self antigens and defects in CD152 presentation and expression has been implicated in a number of autoimmune diseases (Romo-Tena et al. 2013).

This antibody can be used to develop a pharmacokinetic (PK) bridging assay to measure free fusion protein drug abatacept. It is recommended as the capture reagent, paired with human anti abatacept antibody HCA335 as the detection antibody.

Abatacept (Orencia) is a recombinant fusion protein consisting of the extra-cellular domain of human CTLA-4, fused to the modified Fc (hinge, CH2, and CH3 domains) portion of human IgG1.

Target Species
Human
Product Form
Purified IgG - liquid
Preparation
MCA1724GA: Purified IgG prepared by affinity chromatography on Protein A from tissue culture supernatant
MCA1724: Purified by affinity chromatography on Protein A from tissue culture supernatant
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
MCA1724GA: 0.09% sodium azide (NaN3)
MCA1724: 0.09% Sodium Azide (NaN3)
Immunogen
Human CTLA-4/human IgG heavy chain fusion protein.
Approx. Protein Concentrations
IgG concentration 1.0 mg/ml
Fusion Partners
Spleen cells from immunised BALB/c mice were fused with cells of the mouse P3/X63-Ag8.653 myeloma cell line.
Regulatory
For research purposes only
Guarantee
12 months from date of despatch
Acknowledgements
Orencia is a trademark of Bristol-Myers Squibb Company

This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.

Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
ELISA
Flow Cytometry 1 Neat 1/10
Immunofluorescence
Immunohistology - Frozen 2 5ug/ml 50ug/ml
Immunohistology - Paraffin
Immunoprecipitation
  1. 1 Membrane permeabilization is required for this application. The use of Leucoperm (Product Code BUF09) is recommended for this purpose.
  2. 2The epitope recognised by this antibody is reported to be sensitive to formaldehyde fixation and tissue processing. Bio-Rad recommends the use of acetone fixation for frozen sections.
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
Flow Cytometry
Use 10μl of the suggested working dilution to label 1x106 cells in 100μl
ELISA
Mouse anti Human CD152 antibody, clone BNI3 can be used as the capture antibody in a bridging ELISA with HCA335 as the detection antibody.

Protocol: PK bridging ELISA

Protocol: ADA bridging ELISA

Description Product Code Applications Pack Size List Price Your Price Quantity
Goat anti Mouse IgG (H/L):Alk. Phos. (Multi Species Adsorbed) STAR117A E WB 0.5 mg loader
List Price Your Price
loader
Description Goat anti Mouse IgG (H/L):Alk. Phos. (Multi Species Adsorbed)
Goat anti Mouse IgG (H/L):DyLight®488 (Multi Species Adsorbed) STAR117D488GA F IF 0.1 mg loader
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loader
Description Goat anti Mouse IgG (H/L):DyLight®488 (Multi Species Adsorbed)
Goat anti Mouse IgG (H/L):DyLight®550 (Multi Species Adsorbed) STAR117D550 F IF WB 0.1 mg loader
List Price Your Price
loader
Description Goat anti Mouse IgG (H/L):DyLight®550 (Multi Species Adsorbed)
Goat anti Mouse IgG (H/L):DyLight®650 (Multi Species Adsorbed) STAR117D650 F IF 0.1 mg loader
List Price Your Price
loader
Description Goat anti Mouse IgG (H/L):DyLight®650 (Multi Species Adsorbed)
Goat anti Mouse IgG (H/L):DyLight®680 (Multi Species Adsorbed) STAR117D680GA F WB 0.1 mg loader
List Price Your Price
loader
Description Goat anti Mouse IgG (H/L):DyLight®680 (Multi Species Adsorbed)
Goat anti Mouse IgG (H/L):DyLight®800 (Multi Species Adsorbed) STAR117D800GA F IF WB 0.1 mg loader
List Price Your Price
loader
Description Goat anti Mouse IgG (H/L):DyLight®800 (Multi Species Adsorbed)
Goat anti Mouse IgG (H/L):FITC (Multi Species Adsorbed) STAR117F F 0.5 mg loader
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loader
Description Goat anti Mouse IgG (H/L):FITC (Multi Species Adsorbed)
Goat anti Mouse IgG (H/L):HRP (Multi Species Adsorbed) STAR117P C E WB 0.5 mg loader
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loader
Description Goat anti Mouse IgG (H/L):HRP (Multi Species Adsorbed)
Goat anti Mouse IgG (Fc):FITC STAR120F C F 1 mg loader
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loader
Description Goat anti Mouse IgG (Fc):FITC
Goat anti Mouse IgG (Fc):HRP STAR120P E WB 1 mg loader
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loader
Description Goat anti Mouse IgG (Fc):HRP
Rabbit F(ab')2 anti Mouse IgG:RPE STAR12A F 1 ml loader
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loader
Description Rabbit F(ab')2 anti Mouse IgG:RPE
Rabbit F(ab')2 anti Mouse IgG:HRP (Human Adsorbed) STAR13B C E P RE WB 1 mg loader
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loader
Description Rabbit F(ab')2 anti Mouse IgG:HRP (Human Adsorbed)
Goat anti Mouse IgG:FITC (Rat Adsorbed) STAR70 F 0.5 mg loader
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loader
Description Goat anti Mouse IgG:FITC (Rat Adsorbed)
Goat anti Mouse IgG:RPE (Rat Adsorbed) STAR76 F 1 ml loader
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loader
Description Goat anti Mouse IgG:RPE (Rat Adsorbed)
Goat anti Mouse IgG:HRP (Rat Adsorbed) STAR77 C E P 0.5 mg loader
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loader
Description Goat anti Mouse IgG:HRP (Rat Adsorbed)
Goat anti Mouse IgG/A/M:HRP (Human Adsorbed) STAR87P E 1 mg loader
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loader
Description Goat anti Mouse IgG/A/M:HRP (Human Adsorbed)
Rabbit F(ab')2 anti Mouse IgG:FITC STAR9B F 1 mg loader
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loader
Description Rabbit F(ab')2 anti Mouse IgG:FITC

Description Product Code Applications Pack Size List Price Your Price Quantity
Mouse IgG2a Negative Control MCA929 F 100 Tests loader
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loader
Description Mouse IgG2a Negative Control

Description Product Code Applications Pack Size List Price Your Price Quantity
Human anti Abatacept HCA335 E 0.1 mg loader
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loader
Description Human anti Abatacept
Human anti Abatacept HCA336 E 0.1 mg loader
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loader
Description Human anti Abatacept

References for CD152 antibody

  1. Linsley, P.S. et al. (1992) Coexpression and functional cooperation of CTLA-4 and CD28 on activated T lymphocytes.
    J Exp Med. 176 (6): 1595-604.
  2. Kuiper, H.M. et al. (1995) Activated T cells can induce high levels of CTLA-4 expression on B cells.
    J Immunol. 155 (4): 1776-83.
  3. Castan, J. et al. (1997) Accumulation of CTLA-4 expressing T lymphocytes in the germinal centres of human lymphoid tissues.
    Immunology. 90 (2): 265-71.
  4. Steiner, K. et al. (1999) Enhanced expression of CTLA-4 (CD152) on CD4+ T cells in HIV infection.
    Clin Exp Immunol. 115 (3): 451-7.
  5. Steiner, K. et al. (2001) Increased expression of CTLA-4 (CD152) by T and B lymphocytes in Wegener's granulomatosis.
    Clin Exp Immunol. 126: 143-50.
  6. Pistillo, M.P. et al. (2003) CTLA-4 is not restricted to the lymphoid cell lineage and can function as a target molecule for apoptosis induction of leukemic cells.
    Blood. 101: 202-9.
  7. Tan, P.H. et al. (2005) Creation of tolerogenic human dendritic cells via intracellular CTLA4: a novel strategy with potential in clinical immunosuppression.
    Blood. 106: 2936-43.
  8. Lee, C.C. et al. (2009) The regulatory function of umbilical cord blood CD4(+) CD25(+) T cells stimulated with anti-CD3/anti-CD28 and exogenous interleukin (IL)-2 or IL-15.
    Pediatr Allergy Immunol. 20 (7): 624-32.
  9. View The Latest Product References
  10. Ward, F.J. et al. (2013) The soluble isoform of CTLA-4 as a regulator of T-cell responses.
    Eur J Immunol. 43 (5): 1274-85.
  11. Lu, C.H. et al. (2016) DNA Methyltransferase Inhibitor Promotes Human CD4+CD25hFOXP3+ Regulatory T Lymphocyte Induction under Suboptimal TCR Stimulation.
    Front Immunol. 7: 488.

Further Reading

  1. Chin, L.T. et al. (2008) Immune intervention with monoclonal antibodies targeting CD152 (CTLA-4) for autoimmune and malignant diseases.
    Chang Gung Med J. 31: 1-15.

Immunocytochemistry

Immunofluorescence

Synonyms
CTLA-4
RRID
AB_2276878
UniProt
P16410
Entrez Gene
CTLA4
GO Terms
GO:0005887 integral to plasma membrane
GO:0005794 Golgi apparatus
GO:0006955 immune response
GO:0006974 response to DNA damage stimulus
GO:0009897 external side of plasma membrane
GO:0030889 negative regulation of B cell proliferation
GO:0031295 T cell costimulation
GO:0043065 positive regulation of apoptosis
GO:0045334 clathrin-coated endocytic vesicle
GO:0045590 negative regulation of regulatory T cell differentiation
GO:0048471 perinuclear region of cytoplasm
GO:0050853 B cell receptor signaling pathway

MCA1724GA

166248

MCA1724

151633 155886 161456 163655 166060

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