Abatacept antibody | AbD37065ia

Human anti Abatacept

Product Type
Monoclonal Antibody
Clone
AbD37065ia
Isotype
IgG1
Specificity
Abatacept

Product Code Applications Pack Size List Price Your Price Qty
HCA336
Datasheet Datasheet Datasheet
SDS Safety Datasheet SDS
E 0.1 mg loader
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loader
Search for Batch Specific Datasheets

Human Anti-Abatacept, clone AbD37065ia is a recombinant antibody that specifically recognizes the fusion protein drug abatacept as well as cytotoxic T-lymphocyte antigen-4 (CTLA-4), also known as CD152. The antibody binds to free abatacept and can be used to measure free abatacept or abatacept biosimilars in patient samples.

Clone AbD37065ia is a fully human recombinant monoclonal antibody with an IgG1 isotype and is suitable as a surrogate positive control or reference standard in an anti-drug antibody (ADA) assay.

Abatacept (Orencia) is a recombinant fusion protein consisting of the extra-cellular domain of human CTLA-4, fused to the modified Fc (hinge, CH2, and CH3 domains) portion of human IgG1. It is used to treat several autoimmune diseases, including rheumatoid arthritis, psoriatic arthritis and juvenile idiopathic arthritis, by inhibiting optimal T-cell activation. It binds to CD80 and CD86 on antigen-presenting cells and blocks the costimulatory interaction with CD28 on T cells.

View a summary of all anti-abatacept antibodies

Product Form
Human IgG1 antibody (kappa light chain) selected from the HuCAL® phage display library and expressed in a human cell line. This antibody is supplied as a liquid.
Preparation
Purified IgG prepared by affinity chromatography on Protein A
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.01% Thiomersal
Immunogen
Abatacept
Affinity
The monovalent intrinsic affinity of AbD37065ia was measured as KD = 6 nM by real time, label free molecular interaction analysis on immobilized abatacept.
Approx. Protein Concentrations
Antibody concentration 0.5 mg/ml
Regulatory
For research purposes only
Guarantee
12 months from date of despatch
Acknowledgements
This product and/or its use is covered by claims of U.S. patents, and/or pending U.S. and non-U.S. patent applications owned by or under license to Bio-Rad Laboratories, Inc. See bio-rad.com/en-us/trademarks for details.
Orencia is a trademark of Bristol-Myers Squibb Company.

This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.

Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
ELISA
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
Technical Advice
Recommended protocols and further information about HuCAL recombinant antibody technology can be found in the HuCAL Antibodies Technical Manual
ELISA
Clone AbD37065ia can be used in an indirect ELISA. It can also be used as a calibrator in an anti-drug antibody assay.

Protocol: ADA bridging ELISA

Description Product Code Applications Pack Size List Price Your Price Quantity
Hispec Assay Diluent BUF049A E IY 50 ml
List Price Your Price
Description Hispec Assay Diluent
Human anti Abatacept HCA335 E 0.1 mg loader
List Price Your Price
loader
Description Human anti Abatacept
LYNX Rapid HRP Antibody Conjugation Kit LNK002P CJ 3 Conjugations For 400µg Antibody
List Price Your Price
Description LYNX Rapid HRP Antibody Conjugation Kit

Synonyms
Orencia
Licensed Use
For in vitro research purposes and for commercial applications for the provision of in vitro testing services to support preclinical and clinical drug development. Any re-sale in any form or any other commercial application needs a written agreement with Bio-Rad.
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Please Note: All Products are "FOR RESEARCH PURPOSES ONLY"