Actin Gamma antibody | 2A3

Mouse anti Human Actin Gamma

Product Type
Monoclonal Antibody
Clone
2A3
Isotype
IgG2b
Format
Purified
Specificity
Actin Gamma

Product Code Applications Pack Size List Price Your Price Qty
MCA5776GA
Datasheet Datasheet Datasheet
SDS Safety Datasheet SDS
IF P WB 0.1 mg loader
List Price Your Price
loader
Search for Batch Specific Datasheets

Mouse anti Human actin gamma antibody, clone 2A3 recognizes cytoplasmic actin gamma (known also as ACTG and Gamma Actin), a 41.8 kDa cytoskeletal protein.

Actins are highly conserved proteins that are involved in cell motility, structure and integrity and are ubiquitously expressed in all eukaryotic cells. Six different isoforms of actin have been identified (Vandekerckhove and Weber 1978) .

Mouse anti Human actin gamma antibody, clone 2A3 is highly specific for actin gamma and does not react with other actin isoforms (Dugina et al. 2009).
PrecisionAb Antibodies

Target Species
Human
Species Cross-Reactivity
Target SpeciesCross Reactivity
Mammals Expected from Sequence
Mouse
N.B. Antibody reactivity and working conditions may vary between species.
Product Form
Purified IgG - liquid
Preparation
Purified IgG prepared by affinity chromatography on Protein A from tissue culture supernatant
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.09% Sodium Azide (NaN3)
Carrier Free
Yes
Immunogen
Synthetic peptide corresponding to the N-terminal nonapeptide of gamma-cytoplasmic actin, coupled to KLH.
Approx. Protein Concentrations
IgG concentration 1.0 mg/ml
Regulatory
For research purposes only
Guarantee
12 months from date of despatch
Acknowledgements
PrecisionAb is a trademark of Bio-Rad Laboratories

This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.

Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Immunofluorescence
Immunohistology - Paraffin
Western Blotting 1/100 1/1000

The PrecisionAb label is reserved for antibodies that meet the defined performance criteria within Bio-Rad's ongoing antibody validation programme. Click here to learn how we validate our PrecisionAb range. Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.

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References for Actin Gamma antibody

  1. Dugina, V. et al. (2009) Beta and gamma-cytoplasmic actins display distinct distribution and functional diversity.
    J Cell Sci. 122: 2980-8.
  2. Brockmann, C. et al. (2011) Beta- and gamma-cytoplasmic actins are required for meiosis in mouse oocytes.
    Biol Reprod. 85: 1025-39.
  3. Miazza, V. et al. (2011) Sendai virus induced cytoplasmic actin remodeling correlates with efficient virus particle production.
    Virology. 410: 7-16.
  4. Baranwal, S. et al. (2012) Nonredundant roles of cytoplasmic β- and γ-actin isoforms in regulation of epithelial apical junctions.
    Mol Biol Cell. 23: 3542-53.
  5. Dugina, V. et al. (2015) Tumor promotion by γ and suppression by β non-muscle actin isoforms.
    Oncotarget. 6 (16): 14556-71.
  6. Simiczyjew A et al. (2015) Active invadopodia of mesenchymally migrating cancer cells contain both β and γ cytoplasmic actin isoforms.
    Exp Cell Res. pii: S0014-4827(15)30146-4.
  7. Simiczyjew, A. et al. (2017) Involvement of β- and γ-actin isoforms in actin cytoskeleton organization and migration abilities of bleb-forming human colon cancer cells.
    PLoS One. 12 (3): e0173709.
  8. Chaponnier, C. & Gabbiani, G. (2016) Monoclonal antibodies against muscle actin isoforms: epitope identification and analysis of isoform expression by immunoblot and immunostaining in normal and regenerating skeletal muscle.
    F1000Res. 5: 416.
  9. View The Latest Product References
  10. Chen, A. et al. (2017) Cytokinesis requires localized β-actin filament production by an actin isoform specific nucleator.
    Nat Commun. 8 (1): 1530.
  11. Dugina, V. et al. (2021) Impaired Expression of Cytoplasmic Actins Leads to Chromosomal Instability of MDA-MB-231 Basal-Like Mammary Gland Cancer Cell Line.
    Molecules. 26(8):2151.

Immunofluorescence

Western Blotting

Synonyms
ACTG1
RRID
AB_2571583
UniProt
P63261
Entrez Gene
ACTG1
GO Terms
GO:0007596 blood coagulation
GO:0005829 cytosol
GO:0005200 structural constituent of cytoskeleton
GO:0005524 ATP binding
GO:0005856 cytoskeleton
GO:0006928 cellular component movement
GO:0007411 axon guidance
GO:0042802 identical protein binding
GO:0034329 cell junction assembly
GO:0034332 adherens junction organization
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Please Note: All Products are "FOR RESEARCH PURPOSES ONLY"

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