IgE antibody | 7H2

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Mouse anti Horse IgE

Product Type
Monoclonal Antibody
Clone
7H2
Isotype
IgG1
Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
MCA5983GA C E P* WB* 0.1 mg
Mouse anti Horse IgE antibody, clone 7H2 recognizes native equine IgE and does not cross react with equine IgM, IgA or IgG.

IgE is an immunoglobulin primarily produced from plasma cells and, in normal serum, present at very low concentrations. Western blot analysis against affinity purified equine IgE using Mouse anti Horse IgE clone 7H2 demonstrates a single major band of approximately 200 kDa under non-reducing conditions that corresponds with the expected molecular weight of the complete equine IgE molecule (Wilson, D.A. et al. 2006).

IgE is important in both type 1 hypersensitivity and immunity to parasite infections, in particular parasitic worms where equine IgE levels are significantly elevated following worm infection.

Monoclonal antibodies to equine IgE are of particular relevance to research into insect bite sensitivity, one of the most widely studied allergic diseases in equid species (Schaffartzik, A., et al. 2012).

Product Details

Target Species
Horse
Product Form
Purified IgG - liquid
Preparation
Purified IgG prepared by affinity chromatography on Protein A from tissue culture supernatant
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.09% Sodium Azide (NaN3)
Carrier Free
Yes
Immunogen
Equine IgE
Approx. Protein Concentrations
IgG concentration 1.0 mg/ml
Fusion Partners
Spleen cells from immunised Balb/c mice were fused with cells of the P3X myeloma cell line

Storage Information

Storage
Store at +4oC or at -20oC if preferred.
Storage in frost-free freezers is not recommended.
This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Shelf Life
18 months from date of despatch.

More Information

Regulatory
For research purposes only

Applications of IgE antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
ELISA 1/40 1/400
Immunohistology - Frozen
Immunohistology - Paraffin 1
Western Blotting 2
  1. 1 This antibody is demonstrated to work in formalin fixed tissue when using trypsin digestion pre-treatment of paraffin sections. Testing undertaken utilizing heat mediated antigen retrieval techniques with this antibody have not been successful.
  2. 2 Non-reducing conditions are recommended
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
ELISA
This product may be used in an indirect ELISA or as a capture antibody in a sandwich ELISA together with MCA5982P as the detection reagent.
Western Blotting
Clone 7H2 has been demonstrated to detect a band of approximately 200 kDa in non-reducing conditions. This clone is not suitable for use in western blotting under reducing conditions. For western blotting applications it is recommended to use clone 3H10 MCA5982GA.

Secondary Antibodies Available

Description Product Code Pack Size Applications List Price Quantity
Human anti Mouse IgG1:HRP HCA036P 0.1 mg E
Goat anti Mouse IgG (H/L):Alk. Phos. (Multi Species Adsorbed) STAR117A 0.5 mg E WB
Goat anti Mouse IgG (H/L):DyLight®488 (Multi Species Adsorbed) STAR117D488GA 0.1 mg F IF
Goat anti Mouse IgG (H/L):DyLight®549 (Multi Species Adsorbed) STAR117D549GA 0.1 mg F IF WB
Goat anti Mouse IgG (H/L):DyLight®649 (Multi Species Adsorbed) STAR117D649GA 0.1 mg F IF
Goat anti Mouse IgG (H/L):DyLight®680 (Multi Species Adsorbed) STAR117D680GA 0.1 mg F WB
Goat anti Mouse IgG (H/L):DyLight®800 (Multi Species Adsorbed) STAR117D800GA 0.1 mg F IF WB
Goat anti Mouse IgG (H/L):FITC (Multi Species Adsorbed) STAR117F 0.5 mg F
Goat anti Mouse IgG (H/L):HRP (Multi Species Adsorbed) STAR117P 0.5 mg E WB
Goat anti Mouse IgG (Fc):FITC STAR120F 1 mg C F
Goat anti Mouse IgG (Fc):HRP STAR120P 1 mg E WB
Rabbit F(ab')2 anti Mouse IgG:RPE STAR12A 1 ml F
Rabbit F(ab')2 anti Mouse IgG:HRP (Human Adsorbed) STAR13B 1 mg C E P RE WB
Goat anti Mouse IgG:FITC (Rat Adsorbed) STAR70 0.5 mg F
Goat anti Mouse IgG:RPE (Rat Adsorbed) STAR76 1 ml F
Goat anti Mouse IgG:HRP (Rat Adsorbed) STAR77 0.5 mg C E P
Goat anti Mouse IgG/A/M:Alk. Phos. STAR87A 1 mg C E WB
Goat anti Mouse IgG/A/M:HRP (Human Adsorbed) STAR87P 1 mg E
Rabbit F(ab')2 anti Mouse IgG:Dylight®800 STAR8D800GA 0.1 mg F IF WB
Rabbit F(ab')2 anti Mouse IgG:FITC STAR9B 1 mg F

Useful Reagents Available

Description Product Code Pack Size Applications List Price Quantity
Mouse anti Horse IgGb MCA1901GA 0.1 mg E
Mouse anti Horse IgE MCA5982GA 0.1 mg C E WB
Mouse anti Horse IgE:HRP MCA5982P 0.1 mg E
Mouse anti Horse IgA MCA629GA 0.1 mg E

Product Specific References

References for IgE antibody

  1. Wilson, D. A. et al. (2006) Production of monoclonal antibodies specific for native equine IgE and their application to monitor total serum IgE responses in Icelandic and non-Icelandic horses with insect bite dermal hypersensitivity.
    Vet Immunol Immunopathol. 112: 156-70.

Further Reading

  1. Schaffartzik, A.et al. (2012) Equine insect bite hypersensitivity: what do we know?
    Vet Immunol Immunopathol. 147: 113-26