IgE Antibody | 3H10

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IgE Antibody | 3H10 gallery image 1

Purified Horse IgE detected in indirect ELISA with Mouse anti Horse IgE (MCA5982GA) followed by Rabbit F(ab')2 anti Mouse IgG:HRP (STAR13B)

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IgE Antibody | 3H10 gallery image 2

Purified Horse IgE detected in direct ELISA with Mouse anti Horse IgE (MCA5982P)

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  • IgE Antibody | 3H10 thumbnail image 1
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  • Product Type
    Monoclonal Antibody
  • Clone
    3H10
  • Isotype
    IgG1
2 Formats Available
    Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
    MCA5982PEdatasheet pdfdatasheet pdf0.1 mg
    MCA5982P
    MCA5982GAC, E, WBdatasheet pdfdatasheet pdf0.1 mg
    MCA5982GA
    Summary
    Secondary Antibodies
    Negative Isotype Controls
    Useful Reagents
    Positive Controls
    Histology Controls
    More Images
    References
    Reviews
    -
    • Mouse anti Horse IgE, clone 3H10, specifically recognizes native equine IgE and does not cross react with equine IgM, IgA or IgG.

      IgE is an immunoglobulin primarily produced from plasma cells and, in normal serum, present at very low concentrations.

      IgE is important in both type 1 hypersensitivity and immunity to parasite infections, in particular parasitic worms where equine IgE levels are significantly elevated following infection.

      Monoclonal antibodies to equine IgE are of particular relevance to research into insect bite sensitivity, one of the most widely studied allergic diseases in equid species (Schaffartzik, A. et al. 2012).
    • Intended Use
    • Target Species
      Horse
    • Product Form
      Purified IgG conjugated to Horseradish Peroxidase (HRP) - liquid
      Purified IgG - liquid
    • Reconstitution
    • Preparation
      Purified IgG prepared by affinity chromatography on Protein A from tissue culture supernatant
      Purified IgG prepared by affinity chromatography on Protein A from tissue culture supernatant
    • Preservative Stabilisers
      0.01% Thiomersal
      HRP Stabiliser (BUF052A)
      0.09% Sodium Azide (NaN3)
    • Immunogen
      Equine IgE
    • Purity
    • Approx. Protein Concentrations
      IgG concentration1.0 mg/ml
      IgG concentration 1.0 mg/ml
    • Reagents In The Kit
    • Preparing The Antibody
    • Test Principle
    • Buffer Solution
      Phosphate buffered saline
      Phosphate buffered saline
    • Fusion Partners
      Spleen cells from immunised Balb/c mice were fused with cells of the P3X myeloma cell line
    • Storage
      Store at +4oC. DO NOT FREEZE.
      This product should be stored undiluted.
      Store at +4oC or at -20oC if preferred.
      Storage in frost-free freezers is not recommended.
      This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
    • Shelf Life
      18 months from date of despatch
      18 months from date of despatch
    • Acknowledgements
    • Regulatory
      For research purposes only
    • This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.

    • Application NameYesNoMin DilutionMax Dilution
      ELISA1/5001/5,000
    • Application NameYesNoMin DilutionMax Dilution
      ELISA1/10001/10,000
      Immunohistology - Frozen
      Western Blotting

    • Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
    • Technical Advice
    • Technical Advice
    • Recommended Protocol
    • Recommended Protocol
    • ELISA
      This product may be used as a detection reagent in a sandwich ELISA together with MCA5983GA as the capture reagent.
    • ELISA
      This product is suitable for use in indirect ELISA applications.
    • Immunohistology
    • Immunohistology
    • Histology Positive Control Tissue
    • Histology Positive Control Tissue
    • Immunofluorescence
    • Immunofluorescence
    • Western Blotting
    • Western Blotting
      Western blot analysis against affinity purified equine IgE using Mouse anti Horse IgE clone 3H10 demonstrates a band of approximately 80 kDa under reducing conditions and a band of approximately 200 kDa under non-reducing conditions which correspond with the expected molecular weight of equine Ig epsilon chain and the complete equine IgE molecule, respectively (Wilson, D.A. et al. 2006).
    • Instructions For Use
    • Instructions For Use

    Additional IgE Antibody Formats

    Formats Clone Applications Sizes available
    IgE Antibody : HRP 3H10 E 0.1 mg
    IgE Antibody : Purified 3H10 C, E, WB 0.1 mg
    • Copyright © 2016 Bio-Rad

    Recommended Secondary Antibody

      Recommended Negative Isotype Control

        Useful Reagents

          Recommended Positive Controls

            Histology Controls

              References

              Further Reading

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