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IgE antibody | 3H10

Mouse anti Horse IgE:HRP

Product Type
Monoclonal Antibody

Product Code Applications Pack Size List Price Your Price Qty
Datasheet Datasheet Datasheet
SDS Safety Datasheet SDS
E 0.1 mg loader
List Price Your Price

Mouse anti Horse IgE, clone 3H10, recognizes native equine IgE and does not cross react with equine IgM, IgA or IgG.

IgE is an immunoglobulin primarily produced from plasma cells and, in normal serum, present at very low concentrations.

IgE is important in both type 1 hypersensitivity and immunity to parasite infections, in particular parasitic worms where equine IgE levels are significantly elevated following infection.

Monoclonal antibodies to equine IgE are of particular relevance to research into insect bite sensitivity, one of the most widely studied allergic diseases in equid species (Schaffartzik, A. et al. 2012).

Target Species
Product Form
Purified IgG conjugated to Horseradish Peroxidase (HRP) - liquid
Purified IgG prepared by affinity chromatography on Protein A from tissue culture supernatant
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.01% Thiomersal
Equine IgE
Approx. Protein Concentrations
IgG concentration1.0 mg/ml
Fusion Partners
Spleen cells from immunised Balb/c mice were fused with cells of the P3X myeloma cell line
For research purposes only
12 months from date of despatch

This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.

Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
ELISA 1/500 1/5,000
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
This product may be used as a detection reagent in a sandwich ELISA together with MCA5983GA as the capture reagent.

Description Product Code Applications Pack Size List Price Your Price Quantity
Mouse anti Horse IgE MCA5983GA C E P * WB * 0.1 mg loader
List Price Your Price
Description Mouse anti Horse IgE

Source Reference

  1. Wilson, D.A. et al. (2006) Production of monoclonal antibodies specific for native equine IgE and their application to monitor total serum IgE responses in Icelandic and non-Icelandic horses with insect bite dermal hypersensitivity.
    Vet Immunol Immunopathol.112: 56-70.

Further Reading

  1. Schaffartzik, al. (2012) Equine insect bite hypersensitivity: what do we know?
    Vet Immunol Immunopathol. 147: 113-26.


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Please Note: All Products are "FOR RESEARCH PURPOSES ONLY"

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