CD23 antibody | 51-3

Mouse anti Horse CD23

Product Type
Monoclonal Antibody
Clone
51-3
Isotype
IgG1
Specificity
CD23

Product Code Applications Pack Size List Price Your Price Qty
New MCA6394GA
Datasheet Datasheet Datasheet
SDS Safety Datasheet SDS
F 0.1 mg loader
List Price Your Price
loader

Mouse anti Horse CD23 antibody, clone 51-3 recognizes horse CD23 also known as Fc epsilon Receptor II (FcΕRII).

CD23, a C-type lectin, is the low affinity receptor for IgE, but also binds CD21, MHC class II and the integrin alpha-chains CD11b and CD11c. The extracellular domain can be released by proteolytic cleavage and bind ligands such as CD21. In humans and mice, CD23 is found on B-cell subsets and monocytes with low levels of expression on T-cells, follicular dendritic cells, eosinophils, NK-cells, Langerhans cells and platelets (Conrad, 1990). Key functions for CD23 include negative regulation for IgE and IgG antibody responses in B cells by influencing cellular activation and differentiation (Liu et al. 2016) and modulating allergic disease through IgE-facilitated allergen presentation, controlling allergen-specific T-cell activation (Selb et al. 2017).

Mouse anti Horse CD23 antibody, clone 51-3, identified small lymphocytes expressing high levels of MHC class II which were also positive for surface IgE. CD23 was barely detectable on blood T-cells or monocytes. A population with a median CD23 expression of 2.5% (range 0.9–4.5%) was found in equine PBMC (Wagner et al. 2012).

Target Species
Horse
Product Form
Purified IgG - liquid
Preparation
Purified IgG prepared by affinity chromatography on Protein A from tissue culture supernatant
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.09% Sodium Azide (NaN3)
Carrier Free
Yes
Immunogen
Recombinant equine CD23/IL-4 fusion protein
Approx. Protein Concentrations
IgG concentration 1.0 mg/ml
Fusion Partners
Spleen cells from immunised BALB/c mice were fused with cells of the X3.Ag8-653 myeloma cell line
Regulatory
For research purposes only
Guarantee
12 months from date of despatch

This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.

Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Flow Cytometry 1/1000 1/2000
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
Flow Cytometry
Use 10ul of the suggested working dilution to label 1x106 cells in 100ul

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Antibody Characterization Reference

  1. Wagner, B. et al. (2012) Monoclonal antibodies to equine CD23 identify the low-affinity receptor for IgE on subpopulations of IgM+ and IgG1+ B-cells in horses.
    Vet Immunol Immunopathol. 146 (2): 125-34. Vet Immunol Immunopathol. 146 (2): 125-34.

UniProt
A0A3Q2H947

MCA6394GA

163298

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Please Note: All Products are "FOR RESEARCH PURPOSES ONLY"

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