CD23 antibody | 51-3
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Mouse anti Horse CD23
- Product Type
- Monoclonal Antibody
- Clone
- 51-3
- Isotype
- IgG1
- Specificity
- CD23
Antibody Quality and Performance Guarantee
| Mouse anti Horse CD23 antibody, clone 51-3 recognizes horse CD23 also known as Fc epsilon Receptor II (FcΕRII). CD23, a C-type lectin, is the low affinity receptor for IgE, but also binds CD21, MHC class II and the integrin alpha-chains CD11b and CD11c. The extracellular domain can be released by proteolytic cleavage and bind ligands such as CD21. In humans and mice, CD23 is found on B-cell subsets and monocytes with low levels of expression on T-cells, follicular dendritic cells, eosinophils, NK-cells, Langerhans cells and platelets (Conrad, 1990). Key functions for CD23 include negative regulation for IgE and IgG antibody responses in B cells by influencing cellular activation and differentiation (Liu et al. 2016) and modulating allergic disease through IgE-facilitated allergen presentation, controlling allergen-specific T-cell activation (Selb et al. 2017). Mouse anti Horse CD23 antibody, clone 51-3, identified small lymphocytes expressing high levels of MHC class II which were also positive for surface IgE. CD23 was barely detectable on blood T-cells or monocytes. A population with a median CD23 expression of 2.5% (range 0.9–4.5%) was found in equine PBMC (Wagner et al. 2012). |
- Target Species
- Horse
- Product Form
- Purified IgG - liquid
- Preparation
- Purified IgG prepared by affinity chromatography on Protein A from tissue culture supernatant
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
- 0.09% Sodium Azide (NaN3)
- Carrier Free
- Yes
- Immunogen
- Recombinant equine CD23/IL-4 fusion protein
- Approx. Protein Concentrations
- IgG concentration 1.0 mg/ml
- Fusion Partners
- Spleen cells from immunised BALB/c mice were fused with cells of the X3.Ag8-653 myeloma cell line
- Regulatory
- For research purposes only
- Guarantee
- 12 months from date of despatch
This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
| Application Name | Verified | Min Dilution | Max Dilution |
|---|---|---|---|
| Flow Cytometry |  |
1/1000 | 1/2000 |
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
- Flow Cytometry
- Use 10ul of the suggested working dilution to label 1x106 cells in 100ul
Antibody Characterization Reference
-
Wagner, B. et al. (2012) Monoclonal antibodies to equine CD23 identify the low-affinity receptor for IgE on subpopulations of IgM+ and IgG1+ B-cells in horses.
Vet Immunol Immunopathol. 146 (2): 125-34.
- UniProt
- A0A3Q2H947
MCA6394GA
162532
163298
If you cannot find the batch/lot you are looking for please contact our technical support team for assistance.
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