Mouse anti Dog IgG2 antibody, clone CA4F1
recognizes the canine IgG2 heavy chain. ELISA tests have shown that this clone does not react with canine IgG1, IgA or IgM (Arce et al. 2002
). Mouse anti Dog IgG2, clone CA3H1 does not react with the Fab portion of canine IgG or recognize heat, acid and periodate treated canine IgG.
Competition ELISA demonstrated that anti dog IgG2 clones CA3H1 and CA4F1 were able to completely inhibit the binding of each other to IgG2
indicating that both clones recognize the same or closely related epitopes.
- Target Species
- Species Cross-Reactivity
|Target Species||Cross Reactivity|
- N.B. Antibody reactivity and working conditions may vary between species.
- Product Form
- Purified IgG - liquid
- Purified IgG prepared by affinity chromatography on Protein A from tissue culture supernatant
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
- Carrier Free
- Canine IgG
- Approx. Protein Concentrations
- IgG concentration 1.0 mg/ml
- Fusion Partners
- Spleen cells from immunised BALB/c mice were fused with cells of the Sp2/0-Ag14 myeloma cell line
- Store at +4oC or at -20oC if preferred.
Storage in frost-free freezers is not recommended.
This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
- 18 months from date of despatch
- For research purposes only
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Applications of IgG2 antibody
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
- Flow Cytometry
- Use 10ul of the suggested working dilution to label 1x106 cells in 100ul
Copyright © 2020 Bio-Rad Antibodies (formerly AbD Serotec)
Secondary Antibodies Available
Negative Isotype Controls Available
Useful Reagents Available
Product Specific References
References for IgG2 antibody
Arce, C. et al. (2002) Production and characterization of monoclonal antibodies against dog immunoglobulin isotypes.
Vet Immunol Immunopathol. 88 (1-2): 31-41.
Pérez, J. et al. (2002) Comparison of three monoclonal and three polyclonal antibodies in the immunohistochemical diagnosis of canine autoimmune skin diseases.
Vet Dermatol. 13 (5): 231-6.