IgG2 antibody | CA4F1
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F ResetMouse anti Dog IgG2
- Product Type
- Monoclonal Antibody
- Clone
- CA4F1
- Isotype
- IgG1
- Specificity
- IgG2
Quick Links:
Antibody Quality and 
Unrivaled 
| Mouse anti Dog IgG2 antibody, clone CA4F1 recognizes the canine IgG2 heavy chain. ELISA tests have shown that this clone does not react with canine IgG1, IgA or IgM (Arce et al. 2002). Mouse anti Dog IgG2, clone CA4F1 may react with the Fab portion of the canine IgG2 heavy chain. |
- Target Species
- Dog
- Species Cross-Reactivity
-
Target Species Cross Reactivity Goat Rabbit Sheep Human - N.B. Antibody reactivity and working conditions may vary between species.
- Product Form
- Purified IgG - liquid
- Preparation
- Purified IgG prepared by affinity chromatography on Protein A from tissue culture supernatant
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
0.09% Sodium Azide - Carrier Free
- Yes
- Immunogen
- Canine IgG
- Approx. Protein Concentrations
- IgG concentration 1.0 mg/ml
- Fusion Partners
- Spleen cells from immunised BALB/c mice were fused with cells of the Sp2/0-Ag14 myeloma cell line
- Regulatory
- For research purposes only
- Guarantee
- 12 months from date of despatch
This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
| Application Name | Verified | Min Dilution | Max Dilution |
|---|---|---|---|
| ELISA |  |
1/15,000 | 1/150,000 |
| Flow Cytometry | |
||
| Western Blotting | |
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
- Flow Cytometry
- Use 10ul of the suggested working dilution to label 1x106 cells in 100ul
| Description | Product Code | Applications | Pack Size | List Price | Your Price | Quantity | |
|---|---|---|---|---|---|---|---|
| Mouse IgG1 Negative Control | MCA928 | F | 100 Tests |
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| Description | Mouse IgG1 Negative Control | ||||||
| Description | Product Code | Applications | Pack Size | List Price | Your Price | Quantity | |
|---|---|---|---|---|---|---|---|
| Goat anti Dog IgM | AAI30 | C E F | 1 mg |
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| Description | Goat anti Dog IgM | ||||||
| Goat anti Dog IgA | AAI31 | C E ID WB | 1 mg |
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| Description | Goat anti Dog IgA | ||||||
| Goat anti Dog IgG1 | AHP947 | C E WB | 1 mg |
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| Description | Goat anti Dog IgG1 | ||||||
| Sheep anti Dog IgG2 | AHP948 | C E WB | 1 mg |
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| Description | Sheep anti Dog IgG2 | ||||||
| Mouse anti Dog IgE | MCA1895 | E WB | 0.25 mg |
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| Description | Mouse anti Dog IgE | ||||||
| Mouse anti Dog IgE | MCA1896 | E WB | 0.25 mg |
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| Description | Mouse anti Dog IgE | ||||||
| Mouse anti Dog IgA | MCA631GA | E | 0.1 mg |
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| Description | Mouse anti Dog IgA | ||||||
References for IgG2 antibody
-
Arce, C. et al. (2002) Production and characterization of monoclonal antibodies against dog immunoglobulin isotypes.
Vet Immunol Immunopathol. 88 (1-2): 31-41. -
Pérez, J. et al. (2002) Comparison of three monoclonal and three polyclonal antibodies in the immunohistochemical diagnosis of canine autoimmune skin diseases.
Vet Dermatol. 13 (5): 231-6.
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Please Note: All Products are "FOR RESEARCH PURPOSES ONLY"
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