IgG2 antibody | CA4F1
Mouse anti Dog IgG2 antibody, clone CA4F1 recognizes the canine IgG2 heavy chain. ELISA tests have shown that this clone does not react with canine IgG1, IgA or IgM (Arce et al. 2002). Mouse anti Dog IgG2, clone CA4F1 may react with the Fab portion of the canine IgG2 heavy chain.
Product Details
- Target Species
- Dog
- Species Cross-Reactivity
-
Target Species Cross Reactivity Goat Rabbit Sheep Human - N.B. Antibody reactivity and working conditions may vary between species.
- Product Form
- Purified IgG - liquid
- Preparation
- Purified IgG prepared by affinity chromatography on Protein A from tissue culture supernatant
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
0.09% Sodium Azide - Carrier Free
- Yes
- Immunogen
- Canine IgG
- Approx. Protein Concentrations
- IgG concentration 1.0 mg/ml
- Fusion Partners
- Spleen cells from immunised BALB/c mice were fused with cells of the Sp2/0-Ag14 myeloma cell line
Storage Information
- Storage
- Store at +4oC or at -20oC if preferred.
Storage in frost-free freezers is not recommended.
This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use. - Guarantee
- 12 months from date of despatch
More Information
- Regulatory
- For research purposes only
Applications of IgG2 antibody
| Application Name | Verified | Min Dilution | Max Dilution |
|---|---|---|---|
| ELISA | 1/15,000 | 1/150,000 | |
| Flow Cytometry | |||
| Western Blotting |
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
- Flow Cytometry
- Use 10ul of the suggested working dilution to label 1x106 cells in 100ul
Copyright © 2021 Bio-Rad Antibodies (formerly AbD Serotec)
Secondary Antibodies Available
Negative Isotype Controls Available
| Description | Product Code | Applications | Pack Size | List Price | Quantity |
|---|---|---|---|---|---|
| Mouse IgG1 Negative Control | MCA928 | F | 100 Tests |
|
Useful Reagents Available
| Description | Product Code | Applications | Pack Size | List Price | Quantity |
|---|---|---|---|---|---|
| Goat anti Dog IgM | AAI30 | C E F | 1 mg |
|
|
| Goat anti Dog IgA | AAI31 | C E ID WB | 1 mg |
|
|
| Goat anti Dog IgG1 | AHP947 | C E WB | 1 mg |
|
|
| Sheep anti Dog IgG2 | AHP948 | C E WB | 1 mg |
|
|
| Mouse anti Dog IgE | MCA1895 | E WB | 0.25 mg |
|
|
| Mouse anti Dog IgE | MCA1896 | E WB | 0.25 mg |
|
|
| Mouse anti Dog IgA | MCA631GA | E | 0.1 mg |
|
Product Specific References
References for IgG2 antibody
-
Arce, C. et al. (2002) Production and characterization of monoclonal antibodies against dog immunoglobulin isotypes.
Vet Immunol Immunopathol. 88 (1-2): 31-41. -
Pérez, J. et al. (2002) Comparison of three monoclonal and three polyclonal antibodies in the immunohistochemical diagnosis of canine autoimmune skin diseases.
Vet Dermatol. 13 (5): 231-6.
Fluorescent Spectraviewer
Watch the Tool Tutorial Video ▸
How to Use the Spectraviewer?
Watch the Tool Tutorial Video ▸
- Start by selecting the application you are interested in, with the option to select an instrument from the drop down menu or create a customized instrument
- Select the fluorophores or fluorescent proteins you want to include in your panel to check compatibility
- Select the lasers and filters you wish to include
- Select combined or multi-laser view to visualize the spectra
