IgG2 antibody | CA4F1
Mouse anti Dog IgG2 antibody, clone CA4F1 recognizes the canine IgG2 heavy chain. ELISA tests have shown that this clone does not react with canine IgG1, IgA or IgM (Arce et al. 2002). Mouse anti Dog IgG2, clone CA3H1 does not react with the Fab portion of canine IgG or recognize heat, acid and periodate treated canine IgG.
Competition ELISA demonstrated that anti dog IgG2 clones CA3H1 and CA4F1 were able to completely inhibit the binding of each other to IgG2 indicating that both clones recognize the same or closely related epitopes.
Competition ELISA demonstrated that anti dog IgG2 clones CA3H1 and CA4F1 were able to completely inhibit the binding of each other to IgG2 indicating that both clones recognize the same or closely related epitopes.
Product Details
- Target Species
- Dog
- Species Cross-Reactivity
-
Target Species Cross Reactivity Goat Rabbit Sheep Human - N.B. Antibody reactivity and working conditions may vary between species.
- Product Form
- Purified IgG - liquid
- Preparation
- Purified IgG prepared by affinity chromatography on Protein A from tissue culture supernatant
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
0.09% Sodium Azide - Carrier Free
- Yes
- Immunogen
- Canine IgG
- Approx. Protein Concentrations
- IgG concentration 1.0 mg/ml
- Fusion Partners
- Spleen cells from immunised BALB/c mice were fused with cells of the Sp2/0-Ag14 myeloma cell line
Storage Information
- Storage
- Store at +4oC or at -20oC if preferred.
Storage in frost-free freezers is not recommended.
This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use. - Shelf Life
- 18 months from date of despatch
More Information
- Regulatory
- For research purposes only
Applications of IgG2 antibody
| Application Name | Verified | Min Dilution | Max Dilution |
|---|---|---|---|
| ELISA | 1/15,000 | 1/150,000 | |
| Flow Cytometry | |||
| Western Blotting |
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
- Flow Cytometry
- Use 10ul of the suggested working dilution to label 1x106 cells in 100ul
Copyright © 2018 Bio-Rad Antibodies (formerly AbD Serotec)
Secondary Antibodies Available
Negative Isotype Controls Available
| Description | Product Code | Pack Size | Applications | List Price | Quantity |
|---|---|---|---|---|---|
| Mouse IgG1 Negative Control | MCA928 | 100 Tests | F |
Useful Reagents Available
| Description | Product Code | Pack Size | Applications | List Price | Quantity |
|---|---|---|---|---|---|
| Goat anti Dog IgM | AAI30 | 1 mg | C E F | ||
| Goat anti Dog IgA | AAI31 | 1 mg | C E ID WB | ||
| Goat anti Dog IgG1 | AHP947 | 1 mg | C E WB | ||
| Sheep anti Dog IgG2 | AHP948 | 1 mg | C E WB | ||
| Mouse anti Dog IgE | MCA1895 | 0.25 mg | E WB | ||
| Mouse anti Dog IgE | MCA1896 | 0.25 mg | E WB | ||
| Mouse anti Dog IgA | MCA631GA | 0.1 mg | E |
Product Specific References
References for IgG2 antibody
-
Arce, C. et al. (2002) Production and characterization of monoclonal antibodies against dog immunoglobulin isotypes.
Vet Immunol Immunopathol. 88 (1-2): 31-41. -
Pérez, J. et al. (2002) Comparison of three monoclonal and three polyclonal antibodies in the immunohistochemical diagnosis of canine autoimmune skin diseases.
Vet Dermatol. 13 (5): 231-6.
