IL-4

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  • Recombinant Bovine Interleukin-4
(Rated 0.0 out of 5 based on 0 customer reviews)
  • Product Type
    Recombinant Protein
1 Formats Available
    Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
    PBP006E, FNdatasheet pdfdatasheet pdf20 µg
    PBP006
    Summary
    Secondary Antibodies
    Negative Isotype Controls
    Useful Reagents
    Positive Controls
    Histology Controls
    More Images
    References
    Reviews
    -
    • Interleukin-4 (IL-4) is produced by a range of bovine cells, including T and B lymphocytes, monocytes and macrophages, and plays an important role in the immune response.
    • Intended Use
    • Target Species
      Bovine
    • Product Form
      Purified Recombinant Protein - lyophilised
    • Reconstitution
      Reconstitute with 0.5 ml distilled water
      Care should be taken during reconstitution as the protein may appear as a film at the bottom of the vial. Bio-Rad recommend that the vial is gently mixed after reconstitution.

      Further dilutions should be made in a buffer containing carrier protein
    • Preparation
      Recombinant protein expressed in Pichia pastoris
    • Preservative Stabilisers
      None present
    • Purity
      >95% by SDS PAGE analysis
    • Approx. Protein Concentrations
      Total protein concentration 0.04mg/ml after reconstitution
    • Protein Molecular Weight
      12.6kD
    • Reagents In The Kit
    • Preparing The Antibody
    • Test Principle
    • Buffer Solution
      Phosphate buffered saline
    • Storage
      Prior to reconstitution store at +4oC.
      Following reconstitution store at -70oC.
      Storage in frost-free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the protein. Should this product contain a precipitate we recommend microcentrifugation before use.
    • Shelf Life
      12 months from date of reconstitution.
    • GO Terms
      negative regulation of apoptosis
      positive regulation of MHC class II biosynthetic process
      immune response
      positive regulation of B cell proliferation
      extracellular space
      positive regulation of transcription from RNA polymerase II promoter
      B cell activation
      cytokine activity
      interleukin-4 receptor binding
      positive regulation of isotype switching to IgG isotypes
      growth factor activity
      positive regulation of isotype switching to IgE isotypes
      negative regulation of osteoclast differentiation
    • UniProt
    • Entrez Gene
    • Acknowledgements
    • Regulatory
      For research purposes only
    • This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.

    • Application NameYesNoMin DilutionMax Dilution
      ELISA
      Functional Assays

    • Where this protein has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
    • Technical Advice
    • Recommended Protocol
    • ELISA
      PBP006 has been used as a standard in a sandwich ELISA assay with MCA2371 as the capture antibody and MCA2372B for detection.
    • Immunohistology
    • Histology Positive Control Tissue
    • Immunofluorescence
    • Western Blotting
    • Instructions For Use

    Additional IL-4 Formats

    Formats Applications Sizes available
    IL-4 : Rec. Protein E, FN 20 µg
    • Copyright © 2016 Bio-Rad Antibodies (formerly AbD Serotec)

    Recommended Secondary Antibody

      Recommended Negative Isotype Control

        Useful Reagents

          DescriptionProduct CodePack SizeApplicationsList PriceQuantity
          Mouse anti Bovine Interleukin-4:BiotinMCA2372B0.25 mgE
          MCA2372B
          Mouse anti Bovine Interleukin-4MCA23710.5 mgE
          MCA2371

          Recommended Positive Controls

            Histology Controls

              • Application NameReference Images
                Functional Assays

              References

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