IL-4 Antibody | CC314

IL-4 Antibody | CC314 gallery image 1

Published customer image:
Biotinylated Mouse anti Bovine interleukin-4 antibody, clone CC314 (MCA2372B) used as a detection reagen for the evaluation of IL-4 secretion by Lymph dendritic cells in vitro by ELISA in combination with Mouse anti Bovine interleukin-4, clone CC313 (MCA2371) as a capture reagent.
Image caption:
CD1b+ L-DCs are efficient APC for the priming of allogeneic naive T cells. Sorted CD1b+ L-DCs were incubated at different ratios with 105 allogeneic CD4+ T cells (A) or CD62L+ T cells (C), and tested for proliferation by [3H]-thymidine incorporation after six days of culture. Data express the median (quartiles) of counts per minute (cpm) from triplicates for each condition. After three days of culture, supernatants were sampled and IFN-γ (black bars) and IL-4 (grey bars) production analysed by ELISA (B, D). The results shown are from one representative experiment out of four performed on different sheep (A) or out of two performed on one sheep(C).

From: Olivier M, Foret B, Le Vern Y, Guilloteau LA (2012)
Capacities of Migrating CD1b+ Lymph Dendritic Cells to Present Salmonella Antigens to Naive T Cells.
PLoS ONE 7(1): e30430.

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  • Product Type
    Monoclonal Antibody
  • Clone
  • Isotype
1 Formats Available
    Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
    MCA2372BEdatasheet pdfdatasheet pdf0.25 mg
    Secondary Antibodies
    Negative Isotype Controls
    Useful Reagents
    Positive Controls
    Histology Controls
    More Images
    • Mouse anti Bovine interleukin-4 antibody, clone CC314 recognizes bovine intetleukin-4 (IL-4), also known as B-cell stimulatory factor 1. IL-4 is expressed by a range of cells including T and B cells, macrophages and monocytes. It plays an important role in the regulation of bovine T and B cell responses. Bovine interleukin-4 is a 15 kDa, 135 amino acid peptide which is processed to a mature secreted form with the cleavage of a 24 amino acid, N-terminal signal peptide resulting in a 12.6 kDa active cytokine, the apparent Mw may be altered by glycosylation (Heussler et al. 1992).

      IL-4 is expressed by a range of cells including T and B cells, macrophages and monocytes. It plays an important role in the regulation of bovine T and B cell responses.

      Mouse anti Bovine interleukin-4, clone CC314 has been used successfully as a detection antibody in a sandwich ELISA with Mouse anti Bovine IL-4 antibody, clone CC313 (MCA2371) as a coating reagent both with bovine (Stabel et al. 2011) and ovine samples (Olivier et al. 2012). Clones CC313 and CC314 have been used in the reverse configuration for the measurement of interleukin-4 in caprine samples (Marinaro et al. 2012).
    • Intended Use
    • Target Species
    • Species Cross-Reactivity
      Target SpeciesCross Reactivity
      N.B. Antibody reactivity and working conditions may vary between species.
    • Product Form
      Purified IgG conjugated to Biotin - liquid
    • Reconstitution
    • Preparation
      Purified IgG prepared by affinity chromatography on Protein A from tissue culture supernatant
    • Preservative Stabilisers
      0.09%Sodium Azide
      1%Bovine Serum Albumin
    • Immunogen
      Recombinant bovine IL-4.
    • Purity
    • Approx. Protein Concentrations
      IgG concentration 1.0 mg/ml
    • Reagents In The Kit
    • Preparing The Antibody
    • Test Principle
    • Buffer Solution
      Phosphate buffered saline
    • Fusion Partners
      Spleen cells form immunised BALB/c mice were fused with cells of the SP2/0 myeloma cell line.
    • Storage
      Store at +4oC or at -20oC if preferred.
      Storage in frost-free freezers is not recommended.
      This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
    • Shelf Life
      18 months from date of despatch.
    • GO Terms
      positive regulation of transcription from RNA polymerase II promoter
      growth factor activity
      extracellular space
      positive regulation of B cell proliferation
      immune response
      positive regulation of isotype switching to IgE isotypes
      positive regulation of MHC class II biosynthetic process
      B cell activation
      interleukin-4 receptor binding
      cytokine activity
      negative regulation of osteoclast differentiation
      positive regulation of isotype switching to IgG isotypes
      negative regulation of apoptosis
    • UniProt
    • Entrez Gene
    • Acknowledgements
    • Regulatory
      For research purposes only
    • This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.

    • Application NameYesNoMin DilutionMax Dilution

    • Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
    • Technical Advice
    • Recommended Protocol
    • Flow Cytometry
      Use 10ul of the suggested working dilution to label 1x106 cells in 100ul.
    • ELISA
      This biotin conjugate may be used as detection reagent in sandwich ELISA assays for bovine IL-4 with MCA2371 as capture reagent, and recombinant bovine IL-4 (PBP006) as a standard.
    • Immunohistology
    • Histology Positive Control Tissue
    • Immunofluorescence
    • Western Blotting
    • Instructions For Use

    Additional IL-4 Antibody Formats

    Formats Clone Applications Sizes available
    IL-4 Antibody : Biotin CC314 E 0.25 mg
    • Copyright © 2017 Bio-Rad Antibodies (formerly AbD Serotec)

    Recommended Secondary Antibody

      DescriptionProduct CodePack SizeApplicationsList PriceQuantity
      Streptavidin:APCSTAR119100 TestsF

      Recommended Negative Isotype Control

        Useful Reagents

          Recommended Positive Controls

            Histology Controls

              • Application NameReference Images


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