IgA antibody | IL-A71
Mouse anti Bovine IgA, clone IL-A71, is specific for bovine immunoglobulin A (IgA).
IgA is the predominant surface secreted immunoglobulin from the intestinal, urogenital and respiratory tracts. IgA functions in a protective capacity, primarily, by preventing viruses and bacteria from adhering to epithelial tissues. High concentrations of IgA are found in saliva, colostrum, milk, tear duct fluid, urine and nasal fluid.
Clone IL-A71 is reported to immunoprecipitate bands of approximately 62kDa and 75kDa, which is consistent with the heavy chain and the secretory component of IgA (Williams et al. 1990).
In addition to clone IL-A71 which is specific for bovine IgA, monoclonal antibodies recognizing the other major bovine immunoglobulin classes and sub-classes are also available from Bio-Rad.
IgA is the predominant surface secreted immunoglobulin from the intestinal, urogenital and respiratory tracts. IgA functions in a protective capacity, primarily, by preventing viruses and bacteria from adhering to epithelial tissues. High concentrations of IgA are found in saliva, colostrum, milk, tear duct fluid, urine and nasal fluid.
Clone IL-A71 is reported to immunoprecipitate bands of approximately 62kDa and 75kDa, which is consistent with the heavy chain and the secretory component of IgA (Williams et al. 1990).
In addition to clone IL-A71 which is specific for bovine IgA, monoclonal antibodies recognizing the other major bovine immunoglobulin classes and sub-classes are also available from Bio-Rad.
Product Details
- Target Species
- Bovine
- Product Form
- Purified IgG conjugated to Horseradish Peroxidase (HRP) - liquid
- Product Form
- Purified IgG - liquid
- Preparation
- Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant.
- Preparation
- Purified IgG prepared by affinity chromatography on Protein A from tissue culture supernatant
- Buffer Solution
- Phosphate buffered saline
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
0.01% Thiomersal - Preservative Stabilisers
- 0.09% Sodium Azide (NaN3)
- Carrier Free
- Yes
- Immunogen
- Purified bovine Immunoglobulin
- Approx. Protein Concentrations
- IgG concentration 1.0 mg/ml
- Approx. Protein Concentrations
- IgG concentration 1.0 mg/ml
- Fusion Partners
- Spleen cells from immunised BALB/c mice were fused with cells of the X63.Ag8.653 myeloma cell line
Storage Information
- Storage
- This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended. - Storage
- This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended. - Guarantee
- 12 months from date of despatch
- Guarantee
- 12 months from date of despatch
More Information
- Regulatory
- For research purposes only
Applications of IgA antibody
| Application Name | Verified | Min Dilution | Max Dilution |
|---|---|---|---|
| ELISA | |||
| ELISA | 1/50 | 1/5000 | |
| Immunoprecipitation |
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
Copyright © 2021 Bio-Rad Antibodies (formerly AbD Serotec)
Secondary Antibodies Available
Useful Reagents Available
| Description | Product Code | Applications | Pack Size | List Price | Quantity |
|---|---|---|---|---|---|
| AbGUARD® HRP Stabilizer Plus | BUF052A | C E P WB | 100 ml | ||
| AbGUARD® HRP Stabilizer Plus | BUF052B | C E P WB | 500 ml | ||
| AbGUARD® HRP Stabilizer Plus | BUF052C | C E P WB | 1000 ml | ||
| Mouse anti Bovine IgG | MCA2439GA | E IP | 0.1 mg |
|
|
| Mouse anti Bovine IgG1 | MCA2440GA | C E F IP | 0.1 mg |
|
|
| Mouse anti Bovine IgG2 | MCA2441GA | E F IP | 0.1 mg |
|
|
| Mouse anti Bovine IgM | MCA2443GA | E F IP R | 0.1 mg |
|
|
| Mouse anti Sheep IgE | MCA5941GA | C E IF WB | 0.1 mg |
|
Product Specific References
References for IgA antibody
-
Williams, D.J. et al. (1990) Quantitation of bovine immunoglobulin isotypes and allotypes using monoclonal antibodies.
Vet Immunol Immunopathol. 24 (3): 267-83. -
van Dissel, J.T. et al. (2005) Bovine antibody-enriched whey to aid in the prevention of a relapse of Clostridium difficile-associated diarrhoea: preclinical and preliminary clinical data.
J Med Microbiol. 54 (Pt 2): 197-205. -
Sridevi, N.V. et al. (2014) Development of anti-bovine IgA single chain variable fragment and its application in diagnosis of foot-and-mouth disease.
Eur J Microbiol Immunol (Bp). 4 (1): 34-44. -
Hägglund, S. et al. (2011) Bovine respiratory syncytial virus ISCOMs-Immunity, protection and safety in young conventional calves.
Vaccine. 29 (47): 8719-30. -
Mwirigi, M. et al. (2016) Capsular polysaccharide from Mycoplasma mycoides subsp. mycoides shows potential for protection against contagious bovine pleuropneumonia.
Vet Immunol Immunopathol. 178: 64-9. -
Blodörn, K. et al. (2014) Vaccine safety and efficacy evaluation of a recombinant bovine respiratory syncytial virus (BRSV) with deletion of the SH gene and subunit vaccines based on recombinant human RSV proteins: N-nanorings, P and M2-1, in calves with maternal antibodies.
PLoS One. 9 (6): e100392.
Fluorescent Spectraviewer
Watch the Tool Tutorial Video ▸How to Use the Spectraviewer
Watch the Tool Tutorial Video ▸- Start by selecting the application you are interested in, with the option to select an instrument from the drop down menu or create a customized instrument
- Select the fluorophores or fluorescent proteins you want to include in your panel to check compatibility
- Select the lasers and filters you wish to include
- Select combined or multi-laser view to visualize the spectra
