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CD205 antibody | CC98

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Anti Bovine CD205 Antibody, clone CC98 thumbnail image 1 Anti Bovine CD205 Antibody, clone CC98 thumbnail image 2 Anti Bovine CD205 Antibody, clone CC98 thumbnail image 3
Anti Bovine CD205 Antibody, clone CC98 gallery image 1
Published customer image:
Mouse anti Bovine CD205 antibody, clone CC98 (MCA1651GA) used to evaluate CD205 expression on ovine lymph derived dendritic cells by flow cytometry.
Image caption:
CD1b+ L-DCs are migratory mature DC capable of endocytic and phagocytic activities. After labelling lymph cells with anti- CD1b mAb (A), CD1b+ L-cells were gated and analysed for the expression of different markers. Data express the median (quartiles) of the percentage of positive cells from 3 sheep (B). Plots of different markers expressed by CD1b+ L-DCs are shown (C). May-Grünwald Giemsa staining of sorted and cytocentrifugated CD1b+ L-DCs (D). Expression of mRNA for CCR7, CD103 and DC-SIGN by sorted CD1b+ L-DCs (1, 3, 5 respectively) and in cDNA control (2, 4, 6 respectively) (E). Uptake of FITC-ovalbumin (FITC-OVA) or GFP-Salmonella by CD1b+ L- DCs. Lymph cells (1×106 cells) were incubated with FITC-OVA for 1h at 4°C or 37°C, or with GFP-Salmonella for 30 min at 37°C, followed by CD1b labelling. CD1b+ L- DC subset was gated on lymph cells and FITC-OVA fluorescence shown on dot plots F and G. Total L-APC were gated on SSC/FSC and the cell population negative for lymphocyte markers. Quadrants were then defined on the CD1b+ and CD1b- subsets and GFP-Salmonella fluorescence shown on dot plot H.

From: Olivier M, Foret B, Le Vern Y, Guilloteau LA (2012)
Capacities of Migrating CD1b+ Lymph Dendritic Cells to Present Salmonella Antigens to Naive T Cells.
PLoS ONE 7(1): e30430.
Anti Bovine CD205 Antibody, clone CC98 gallery image 2
Published customer image:
Mouse anti Bovine CD205 antibody, clone CC98 (MCA1651GA) used to evaluate CD205 expression on ovine lymph derived dendritic cells by flow cytometry.
Image caption:
Presentation of Salmonella antigens to CD4+ T cells by CD1b+ L-DCs primed by conjunctival vaccination with the Salmonella Rv-6 strain. After labelling of cervical lymph cells with anti- CD1b mAb, CD1b+ L-cells were gated and analysed for the expression of different markers. Data express the median (quartiles) of the percentage of positive cells from three sheep (A). Plots of different markers expressed by cervical CD1b+ L-DCs are shown (B). Sorted cervical CD1b+ L-DCs were isolated from sheep before and after conjunctival vaccination with Salmonella, and co-cultured with autologous purified CD4+ T cells at a ratio of 1:100. Cells were co-cultured for six days and the proliferative response assessed by [3H]-thymidine incorporation (B). Data express the median (quartiles) of counts per minute (cpm) from triplicates for each condition. The results shown represent three independent experiments performed on one sheep.

From: Olivier M, Foret B, Le Vern Y, Guilloteau LA (2012)
Capacities of Migrating CD1b+ Lymph Dendritic Cells to Present Salmonella Antigens to Naive T Cells.
PLoS ONE 7(1): e30430.
Anti Bovine CD205 Antibody, clone CC98 gallery image 3
Published customer image:
Mouse anti Bocine CD205 antibody, clone CC98 (MCA1651GA) used for the identification of denritic cells in bovine subcutaneous tissues and lymph nodes along with Mouse anti Human CD14 antibody, clone Tük14 (MCA1568GA) to identify monocytic cells by double immunofluorescent staining on cryostat sections.
Image caption:
Distribution of monocyte and DC markers in skin and LNs. Immunofluorescent labeling of subcutaneous tissues and LNs with antibodies against CD14 (green), CD205 (red) and CD11c (blue), at 24 h post-injection. (A) Large numbers of CD14+ cells were observed in the subcutis on the injected side. Note the separation of collagen bundles (appears as gray auto fluorescence, arrowheads) due to inflammatory infiltrates. A limited number of CD205+ cells were present. (B) A few CD14+ cells (arrow) were observed on the contralateral side. (C) CD14+ cells infiltrated the interfollicular areas of the cortex of the draining LN. A moderate amount of CD205+ CD11c+ DCs were observed (arrow heads in insert). (D) CD14+ cells were sparsely present in the contralateral LN. CD205+ follicles were surrounded by CD11c single labeled and CD11c+ CD205+ double labeled cells. Follicle (f), interfollicular area (i), capsule (c), sinus area (s). Scale bars: 100 μm.

From: Lund H, Boysen P, Åkesson CP, Lewandowska-Sabat AM, Storset AK.
Transient Migration of Large Numbers of CD14(++) CD16(+) Monocytes to the Draining Lymph Node after Onset of Inflammation.
Front Immunol. 2016 Aug 29;7:322.

Mouse anti Bovine CD205:FITC

Mouse anti Bovine CD205

Product Type
Monoclonal Antibody
Clone
CC98
Isotype
IgG2b
Product Code Applications Pack Size List Price Quantity
MCA1651F
Datasheet
F
MSDS
0.1 mg loader

MCA1651GA
Datasheet
C F IP
MSDS
0.1 mg loader

Mouse anti Bovine CD205 antibody, clone CC98 recognizes the bovine CD205 cell surface antigen, a ~210-220 kDa molecule expressed by T cells that are CD2+ve but not WC1+ve. CD205 is also expressed by B cells, and weakly stains B cell follicles.

Bovine CD205 has previously been described as the WC6 antigen (Gliddon et al. 2004).

Dendritic cells (veiled cells) in afferent lymph are strong expressors of CD205 as are dendritic cells in various other tissues.

Product Details

Target Species
Bovine
Species Cross-Reactivity
Target SpeciesCross Reactivity
Sheep
N.B. Antibody reactivity and working conditions may vary between species.
Product Form
Purified IgG conjugated to Fluorescein Isothiocyanate Isomer 1 (FITC) - liquid
Product Form
Purified IgG - liquid
Preparation
Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
Preparation
Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
Buffer Solution
Phosphate buffered saline pH7.4
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.09%Sodium Azide
1%Bovine Serum Albumin
Preservative Stabilisers
0.09%Sodium Azide
Carrier Free
Yes
Approx. Protein Concentrations
IgG concentration 0.1 mg/ml
Approx. Protein Concentrations
IgG concentration 1.0 mg/ml

Storage Information

Storage
Store at +4oC or at -20oC if preferred.

This product should be stored undiluted.

Storage in frost free freezers is not recommended. This product is photosensitive and should be protected from light.
Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Storage
Store at +4oC or at -20oC if preferred.

This product should be stored undiluted.

Storage in frost free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Guarantee
18 months from date of despatch.
Guarantee
18 months from date of despatch.

More Information

Regulatory
For research purposes only

Applications of CD205 antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Flow Cytometry Neat 1/10
Flow Cytometry 1/50 1/200
Immunohistology - Frozen
Immunoprecipitation
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
Flow Cytometry
Use 10ul of the suggested working dilution to label 106 cells in 100ul.
Flow Cytometry
Use 10ul of the suggested working dilution to label 106 cells in 100ul.
Copyright © 2019 Bio-Rad Antibodies (formerly AbD Serotec)

Secondary Antibodies Available

Description Product Code Applications Pack Size List Price Quantity
Human anti Mouse IgG2b:FITC HCA038F F 0.1 mg loader
Goat anti Mouse IgG (H/L):Alk. Phos. (Multi Species Adsorbed) STAR117A E WB 0.5 mg loader
Goat anti Mouse IgG (H/L):DyLight®488 (Multi Species Adsorbed) STAR117D488GA F IF 0.1 mg loader
Goat anti Mouse IgG (H/L):DyLight®680 (Multi Species Adsorbed) STAR117D680GA F WB 0.1 mg loader
Goat anti Mouse IgG (H/L):DyLight®800 (Multi Species Adsorbed) STAR117D800GA F IF WB 0.1 mg loader
Goat anti Mouse IgG (H/L):FITC (Multi Species Adsorbed) STAR117F F 0.5 mg loader
Goat anti Mouse IgG (H/L):HRP (Multi Species Adsorbed) STAR117P E WB 0.5 mg loader
Goat anti Mouse IgG (Fc):FITC STAR120F C F 1 mg loader
Goat anti Mouse IgG (Fc):HRP STAR120P E WB 1 mg loader
Rabbit F(ab')2 anti Mouse IgG:RPE STAR12A F 1 ml loader
Rabbit F(ab')2 anti Mouse IgG:HRP (Human Adsorbed) STAR13B C E P RE WB 1 mg loader
Goat anti Mouse IgG:FITC (Rat Adsorbed) STAR70 F 0.5 mg loader
Goat anti Mouse IgG:RPE (Rat Adsorbed) STAR76 F 1 ml loader
Goat anti Mouse IgG:HRP (Rat Adsorbed) STAR77 C E P 0.5 mg loader
Goat anti Mouse IgG/A/M:Alk. Phos. STAR87A C E WB 1 mg loader
Goat anti Mouse IgG/A/M:HRP (Human Adsorbed) STAR87P E 1 mg loader
Rabbit F(ab')2 anti Mouse IgG:Dylight®800 STAR8D800GA F IF WB 0.1 mg loader
Rabbit F(ab')2 anti Mouse IgG:FITC STAR9B F 1 mg loader

Negative Isotype Controls Available

Description Product Code Applications Pack Size List Price Quantity
Mouse IgG2b Negative Control:FITC MCA691F F 100 Tests loader
Mouse IgG2b Negative Control MCA691 F 100 Tests loader

Application Based External Images

Flow Cytometry

Immunohistology - Frozen

Product Specific References

References for CD205 antibody

  1. Howard, C.J. & Naessens, J. (1993) Summary of workshop findings for cattle (tables 1 and 2).
    Vet Immunol Immunopathol. 39 (1-3): 25-47.
  2. Howard, C.J. et al. (1996) Afferent lymph veiled cells stimulate proliferative responses in allogeneic CD4+ and CD8+ T cells but not gamma delta TCR+ T cells.
    Immunology. 88 (4): 558-64.
  3. Naessens, J. et al. (1993) Cross-reactivity of workshop antibodies with cells from domestic and wild ruminants.
    Vet Immunol Immunopathol. 39 (1-3): 283-90.
  4. Gliddon, D.R. et al. (2004) DEC-205 expression on migrating dendritic cells in afferent lymph.
    Immunology. 111 (3): 262-72.
  5. Akesson, C.P. et al. (2008) Phenotypic characterisation of intestinal dendritic cells in sheep.
    Dev Comp Immunol. 32: 837-49.
  6. Ferret-Bernard, S. et al. (2011) Mesenteric lymph node cells from neonates present a prominent IL-12 response to CpG oligodeoxynucleotide via an IL-15 feedback loop of amplification.
    Vet Res. 42:19.
  7. Ferret-Bernard, S. et al. (2010) Cellular and molecular mechanisms underlying the strong neonatal IL-12 response of lamb mesenteric lymph node cells to R-848.
    PLoS One. 5: e13705.
  8. Fach, S.J. et al. (2007) Neonatal ovine pulmonary dendritic cells support bovine respiratory syncytial virus replication with enhanced interleukin (IL)-4 And IL-10 gene transcripts.
    Viral Immunol. 20: 119-30.
  9. Eicher, S.D. et al. (2011) β-Glucan plus ascorbic acid in neonatal calves modulates immune functions with and without Salmonella enterica serovar Dublin.
    Vet Immunol Immunopathol. 142: 258-64.
  10. Olivier, M. et al. (2012) Capacities of Migrating CD1b Lymph Dendritic Cells to Present Salmonella Antigens to Naive T Cells
    PLoS One. 7: e30430.
  11. Thonur, L. et al. (2012) Toll-like receptor gene expression in fresh and archived ovine pseudoafferent lymph DEC205+ dendritic cells.
    J Comp Pathol. 147 (2-3): 296-304.
  12. Sigmundsdottir, H. et al. (2007) DCs metabolize sunlight-induced vitamin D3 to 'program' T cell attraction to the epidermal chemokine CCL27.
    Nat Immunol. 8: 285-93.
  13. Fach, S.J. et al. (2007) Neonatal ovine pulmonary dendritic cells support bovine respiratory syncytial virus replication with enhanced interleukin (IL)-4 And IL-10 gene transcripts.
    Viral Immunol. 20: 119-30.
  14. McNeilly, T.N. et al. (2006) Differential expression of cell surface markers by ovine respiratory tract dendritic cells.
    J Histochem Cytochem. 54: 1021-30.
  15. Walters, A.A. et al. (2015) Assessment of the enhancement of PLGA nanoparticle uptake by dendritic cells through the addition of natural receptor ligands and monoclonal antibody.
    Vaccine. 33 (48): 6588-95.
  16. Lund, H. et al. (2016) Transient Migration of Large Numbers of CD14(++) CD16(+) Monocytes to the Draining Lymph Node after Onset of Inflammation.
    Front Immunol. 7: 322.
  17. Uhde, A-K. et al. (2017) Evaluation of a panel of antibodies for the immunohistochemical identification of immune cells in paraffin-embedded lymphoid tissues of new- and old-world camelids
    Vet Immunol Immunopathol. Jan 17 [Epub ahead of print]
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