CD205 antibody | CC98
Mouse anti Bovine CD205 antibody, clone CC98 (MCA1651GA) used to evaluate CD205 expression on ovine lymph derived dendritic cells by flow cytometry.
Image caption:
CD1b+ L-DCs are migratory mature DC capable of endocytic and phagocytic activities. After labelling lymph cells with anti- CD1b mAb (A), CD1b+ L-cells were gated and analysed for the expression of different markers. Data express the median (quartiles) of the percentage of positive cells from 3 sheep (B). Plots of different markers expressed by CD1b+ L-DCs are shown (C). May-Grünwald Giemsa staining of sorted and cytocentrifugated CD1b+ L-DCs (D). Expression of mRNA for CCR7, CD103 and DC-SIGN by sorted CD1b+ L-DCs (1, 3, 5 respectively) and in cDNA control (2, 4, 6 respectively) (E). Uptake of FITC-ovalbumin (FITC-OVA) or GFP-Salmonella by CD1b+ L- DCs. Lymph cells (1×106 cells) were incubated with FITC-OVA for 1h at 4°C or 37°C, or with GFP-Salmonella for 30 min at 37°C, followed by CD1b labelling. CD1b+ L- DC subset was gated on lymph cells and FITC-OVA fluorescence shown on dot plots F and G. Total L-APC were gated on SSC/FSC and the cell population negative for lymphocyte markers. Quadrants were then defined on the CD1b+ and CD1b- subsets and GFP-Salmonella fluorescence shown on dot plot H.
From: Olivier M, Foret B, Le Vern Y, Guilloteau LA (2012)
Capacities of Migrating CD1b+ Lymph Dendritic Cells to Present Salmonella Antigens to Naive T Cells.
PLoS ONE 7(1): e30430.
Mouse anti Bovine CD205 antibody, clone CC98 (MCA1651GA) used to evaluate CD205 expression on ovine lymph derived dendritic cells by flow cytometry.
Image caption:
Presentation of Salmonella antigens to CD4+ T cells by CD1b+ L-DCs primed by conjunctival vaccination with the Salmonella Rv-6 strain. After labelling of cervical lymph cells with anti- CD1b mAb, CD1b+ L-cells were gated and analysed for the expression of different markers. Data express the median (quartiles) of the percentage of positive cells from three sheep (A). Plots of different markers expressed by cervical CD1b+ L-DCs are shown (B). Sorted cervical CD1b+ L-DCs were isolated from sheep before and after conjunctival vaccination with Salmonella, and co-cultured with autologous purified CD4+ T cells at a ratio of 1:100. Cells were co-cultured for six days and the proliferative response assessed by [3H]-thymidine incorporation (B). Data express the median (quartiles) of counts per minute (cpm) from triplicates for each condition. The results shown represent three independent experiments performed on one sheep.
From: Olivier M, Foret B, Le Vern Y, Guilloteau LA (2012)
Capacities of Migrating CD1b+ Lymph Dendritic Cells to Present Salmonella Antigens to Naive T Cells.
PLoS ONE 7(1): e30430.
Mouse anti Bocine CD205 antibody, clone CC98 (MCA1651GA) used for the identification of denritic cells in bovine subcutaneous tissues and lymph nodes along with Mouse anti Human CD14 antibody, clone Tük14 (MCA1568GA) to identify monocytic cells by double immunofluorescent staining on cryostat sections.
Image caption:
Distribution of monocyte and DC markers in skin and LNs. Immunofluorescent labeling of subcutaneous tissues and LNs with antibodies against CD14 (green), CD205 (red) and CD11c (blue), at 24 h post-injection. (A) Large numbers of CD14+ cells were observed in the subcutis on the injected side. Note the separation of collagen bundles (appears as gray auto fluorescence, arrowheads) due to inflammatory infiltrates. A limited number of CD205+ cells were present. (B) A few CD14+ cells (arrow) were observed on the contralateral side. (C) CD14+ cells infiltrated the interfollicular areas of the cortex of the draining LN. A moderate amount of CD205+ CD11c+ DCs were observed (arrow heads in insert). (D) CD14+ cells were sparsely present in the contralateral LN. CD205+ follicles were surrounded by CD11c single labeled and CD11c+ CD205+ double labeled cells. Follicle (f), interfollicular area (i), capsule (c), sinus area (s). Scale bars: 100 μm.
From: Lund H, Boysen P, Åkesson CP, Lewandowska-Sabat AM, Storset AK.
Transient Migration of Large Numbers of CD14(++) CD16(+) Monocytes to the Draining Lymph Node after Onset of Inflammation.
Front Immunol. 2016 Aug 29;7:322.
Mouse anti Bovine CD205 antibody, clone CC98 recognizes the bovine CD205 cell surface antigen, a ~210-220 kDa molecule expressed by T cells that are CD2+ve but not WC1+ve. CD205 is also expressed by B cells, and weakly stains B cell follicles.
Bovine CD205 has previously been described as the WC6 antigen (Gliddon et al. 2004).
Dendritic cells (veiled cells) in afferent lymph are strong expressors of CD205 as are dendritic cells in various other tissues.
Bovine CD205 has previously been described as the WC6 antigen (Gliddon et al. 2004).
Dendritic cells (veiled cells) in afferent lymph are strong expressors of CD205 as are dendritic cells in various other tissues.
Product Details
- Target Species
- Bovine
- Species Cross-Reactivity
-
Target Species Cross Reactivity Sheep - N.B. Antibody reactivity and working conditions may vary between species.
- Product Form
- Purified IgG conjugated to Fluorescein Isothiocyanate Isomer 1 (FITC) - liquid
- Product Form
- Purified IgG - liquid
- Preparation
- Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
- Preparation
- Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
- Buffer Solution
- Phosphate buffered saline pH7.4
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
0.09% Sodium Azide 1% Bovine Serum Albumin - Preservative Stabilisers
0.09% Sodium Azide - Carrier Free
- Yes
- Approx. Protein Concentrations
- IgG concentration 0.1 mg/ml
- Approx. Protein Concentrations
- IgG concentration 1.0 mg/ml
Storage Information
- Storage
- Store at +4oC or at -20oC if preferred.
This product should be stored undiluted.
Storage in frost free freezers is not recommended. This product is photosensitive and should be protected from light.
Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use. - Storage
- Store at +4oC or at -20oC if preferred.
This product should be stored undiluted.
Storage in frost free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use. - Shelf Life
- 18 months from date of despatch.
- Shelf Life
- 18 months from date of despatch.
More Information
- Regulatory
- For research purposes only
Applications of CD205 antibody
| Application Name | Verified | Min Dilution | Max Dilution |
|---|---|---|---|
| Flow Cytometry | Neat | 1/10 | |
| Flow Cytometry | 1/50 | 1/200 | |
| Immunohistology - Frozen | |||
| Immunoprecipitation |
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
- Flow Cytometry
- Use 10ul of the suggested working dilution to label 106 cells in 100ul.
- Flow Cytometry
- Use 10ul of the suggested working dilution to label 106 cells in 100ul.
Copyright © 2018 Bio-Rad Antibodies (formerly AbD Serotec)
Secondary Antibodies Available
Negative Isotype Controls Available
| Description | Product Code | Pack Size | Applications | List Price | Quantity |
|---|---|---|---|---|---|
| Mouse IgG2b Negative Control:FITC | MCA691F | 100 Tests | F | ||
| Mouse IgG2b Negative Control | MCA691 | 100 Tests | F |
Product Specific References
References for CD205 antibody
-
Howard, C.J. & Naessens, J. (1993) Summary of workshop findings for cattle (tables 1 and 2).
Vet Immunol Immunopathol. 39 (1-3): 25-47. -
Howard, C.J. et al. (1996) Afferent lymph veiled cells stimulate proliferative responses in allogeneic CD4+ and CD8+ T cells but not gamma delta TCR+ T cells.
Immunology. 88 (4): 558-64. -
Naessens, J. et al. (1993) Cross-reactivity of workshop antibodies with cells from domestic and wild ruminants.
Vet Immunol Immunopathol. 39 (1-3): 283-90. -
Gliddon, D.R. et al. (2004) DEC-205 expression on migrating dendritic cells in afferent lymph.
Immunology. 111 (3): 262-72. -
Akesson, C.P. et al. (2008) Phenotypic characterisation of intestinal dendritic cells in sheep.
Dev Comp Immunol. 32: 837-49. -
Ferret-Bernard, S. et al. (2011) Mesenteric lymph node cells from neonates present a prominent IL-12 response to CpG oligodeoxynucleotide via an IL-15 feedback loop of amplification.
Vet Res. 42:19. -
Ferret-Bernard, S. et al. (2010) Cellular and molecular mechanisms underlying the strong neonatal IL-12 response of lamb mesenteric lymph node cells to R-848.
PLoS One. 5: e13705. -
Fach, S.J. et al. (2007) Neonatal ovine pulmonary dendritic cells support bovine respiratory syncytial virus replication with enhanced interleukin (IL)-4 And IL-10 gene transcripts.
Viral Immunol. 20: 119-30. -
Eicher, S.D. et al. (2011) β-Glucan plus ascorbic acid in neonatal calves modulates immune functions with and without Salmonella enterica serovar Dublin.
Vet Immunol Immunopathol. 142: 258-64. -
Olivier, M. et al. (2012) Capacities of Migrating CD1b Lymph Dendritic Cells to Present Salmonella Antigens to Naive T Cells
PLoS One. 7: e30430. -
Thonur, L. et al. (2012) Toll-like receptor gene expression in fresh and archived ovine pseudoafferent lymph DEC205+ dendritic cells.
J Comp Pathol. 147 (2-3): 296-304. -
Sigmundsdottir, H. et al. (2007) DCs metabolize sunlight-induced vitamin D3 to 'program' T cell attraction to the epidermal chemokine CCL27.
Nat Immunol. 8: 285-93. -
Fach, S.J. et al. (2007) Neonatal ovine pulmonary dendritic cells support bovine respiratory syncytial virus replication with enhanced interleukin (IL)-4 And IL-10 gene transcripts.
Viral Immunol. 20: 119-30. -
McNeilly, T.N. et al. (2006) Differential expression of cell surface markers by ovine respiratory tract dendritic cells.
J Histochem Cytochem. 54: 1021-30. -
Walters, A.A. et al. (2015) Assessment of the enhancement of PLGA nanoparticle uptake by dendritic cells through the addition of natural receptor ligands and monoclonal antibody.
Vaccine. 33 (48): 6588-95. -
Lund, H. et al. (2016) Transient Migration of Large Numbers of CD14(++) CD16(+) Monocytes to the Draining Lymph Node after Onset of Inflammation.
Front Immunol. 7: 322. -
Uhde, A-K. et al. (2017) Evaluation of a panel of antibodies for the immunohistochemical identification of immune cells in paraffin-embedded lymphoid tissues of new- and old-world camelids
Vet Immunol Immunopathol. Jan 17 [Epub ahead of print]
