Actin Gamma Antibody | 2A3

Western blot analysis of NIH 3T3 murine embryonic fibroblast whole cell lysate probed with Mouse anti Human actin gamma antibody (MCA5776GA) followed by HRP conjugated Goat anti Nouse IgG, visualized by chemiluminescence

Published customer image:
Mouse anti Human Actin gamma antibody, clone 2A3 used for the evaluation of γ-actin expression in normal and neoplasic cells by immunofluorescence and western blotting.
Image caption:
Expression of cytoplasmic actins in normal and transformed cells (A-C); expression of cytoplasmic actins and HaCaT cellular characteristics (D-G) A. Immunofluorescent staining for β-actin (green) and γ-actin (red) of representative pairs of NSCLC and Colon Cancer samples with matching normal tissue samples. Scale bars represent 50μm. Graphs represent staining intensity of biopsies (Mean ± SD). B. WB analysis of HaCaT cells with exogenous expression of active N-RasD13. Graphs represent relative actins expression (Mean ± SD). C. Immunofluorescent staining for β-actin (green) and γ-actin (red) of N-RasD13 transformed HaCaT cells. Scale bars represent 10 μm. D. WB analysis of HaCaT cells with exogenous expression of β- or γ-actins and corresponding shRNAs. shC is sh to GFP. Graphs represent relative actins expression (Mean ± SD). E. Semi-quantitative RT-PCR analysis of β- or γ-actins expression in cells with corresponding shRNAs. Graphs represent folds of RNA expression in comparison to control cells (Mean ± SD). F. Immunofluorescent staining of HaCaT cells with altered β- or γ-actins expression. Scale bars represent 10 μm. G. HaCaT cells with silenced or exogenously expressed non-muscle cytoplasmic actins that crossed the matrigel-coated membranes (left). Graphs represent mean ± SD. HaCaT proliferation dynamics with exogenous expression or silenced of β- or γ-actins (right). Error bars are SD.

From: Dugina V, Khromova N, Rybko V, Blizniukov O, Shagieva G, Chaponnier C, Kopnin B, Kopnin P. Tumor promotion by γ and suppression by β non-muscle actin isoforms.
Oncotarget. 2015 Jun 10;6(16):14556-71.

Published customer image:
Mouse anti Human Actin gamma antibody, clone 2A3 used for the evaluation of γ-actin expression in cell lines by immunofluorescence and western blotting.
Image caption:
β- and γ-actins in neoplastic cells phenotype A. WB analysis of A549 cells (left panel) and HCT116 cells (right panel) with exogenous expression of β- or γ-actins and corresponding shRNAs. Graphs represent relative actins expression (Mean ± SD). B. Semi-quantitative RT-PCR analysis of β- or γ-actins expression in A549 (left panel) and HCT116 (right panel) with corresponding shRNAs. Graphs represent folds of RNA expression in comparison to control cells (Mean ± SD). C. Immunofluorescent staining of A549 cells (left panel) and HCT116 cells (right panel) with exogenous expression of β- or γ-actins and corresponding shRNAs. Scale bars represent 10 μm.

From: Dugina V, Khromova N, Rybko V, Blizniukov O, Shagieva G, Chaponnier C, Kopnin B, Kopnin P. Tumor promotion by γ and suppression by β non-muscle actin isoforms.
Oncotarget. 2015 Jun 10;6(16):14556-71.

Published customer image:
Mouse anti Human Actin gamma antibody, clone 2A3 used for the evaluation of γ-actin expression in cell lines by immunofluorescence and western blotting.
Image caption:
β- and γ-actins in neoplastic cells phenotype A. WB analysis of A549 cells (left panel) and HCT116 cells (right panel) with exogenous expression of β- or γ-actins and corresponding shRNAs. Graphs represent relative actins expression (Mean ± SD). B. Semi-quantitative RT-PCR analysis of β- or γ-actins expression in A549 (left panel) and HCT116 (right panel) with corresponding shRNAs. Graphs represent folds of RNA expression in comparison to control cells (Mean ± SD). C. Immunofluorescent staining of A549 cells (left panel) and HCT116 cells (right panel) with exogenous expression of β- or γ-actins and corresponding shRNAs. Scale bars represent 10 μm.

From: Dugina V, Khromova N, Rybko V, Blizniukov O, Shagieva G, Chaponnier C, Kopnin B, Kopnin P. Tumor promotion by γ and suppression by β non-muscle actin isoforms.
Oncotarget. 2015 Jun 10;6(16):14556-71.

Published customer image:
Mouse anti Human Actin gamma antibody, clone 2A3 used for the evaluation of γ-actin expression in A549 cells by immunofluorescence and western blotting.
Image caption:
γ-actin and ERK1/2 are mutually regulated A. WB analysis of A549 cells with exogenous expression of β- or γ-actins and corresponding shRNAs. Graphs represent relative pERK1/2 expression (Mean ± SD). B. LSM of A549 cells with down-regulated β- or γ-actins with β-actin (green), γ-actin (purple) or pERK1/2 (red) immunofluorescent staining. Scale bars represent 10 μm. C. pERK1/2 immunoprecipitation analysis of A549 cells with down-regulated β- or γ-actins. D. pERK1/2/γ-actin PLA analysis of A549 cells with down-regulated β- or γ-actins. Scale bar represents 10 μm. Graph represents relative fluorescence intensity (Mean ± SD). E. LSM of A549 cells treated with EGF or UO126 with β-actin (green) or γ-actin (red) immunofluorescent staining. Scale bars represent 10 μm. F. WB analysis of A549 cells treated with EGF or UO126. Graphs represent relative pERK1/2 or β-/γ-actin levels (Mean ± SD).

From: Dugina V, Khromova N, Rybko V, Blizniukov O, Shagieva G, Chaponnier C, Kopnin B, Kopnin P. Tumor promotion by γ and suppression by β non-muscle actin isoforms.
Oncotarget. 2015 Jun 10;6(16):14556-71.

Published customer image:
Mouse anti Human Actin gamma antibody, clone 2A3 used for the evaluation of γ-actin expression in A549 cells by immunofluorescence and western blotting.
Image caption:
γ-actin co-localizes with p34-Arc and WAVE2 and regulates them A. p34-Arc (red) or γ-actin (green) immunofluorescence images of A549 cells with down-regulated β- or γ-actins. Scale bars represent 10 μm. B. γ-Actin (red) or WAVE2 (green) immunofluorescence images of A549 cells with down-regulated β- or γ-actins. Scale bars represent 10 μm. C. WB analysis of A549 cells with down-regulated β- or γ-actins. Graphs represent relative protein levels (Mean ± SD). D. p34-Arc immunoprecipitation analysis of A549 cells with down-regulated β- or &gamma-actins. E. WAVE2 immunoprecipitation analysis of A549 cells with down-regulated β- or γ-actins. F. LSM of A549 cells with down-regulated β-actin with p34-Arc (green, upper panel), WAVE2 (green, lower panel), β-actin (red) or γ-actin (purple) immunofluorescent staining. Scale bars represent 2 μm. Asterisk marks the cell with silenced β-actin.

From: Dugina V, Khromova N, Rybko V, Blizniukov O, Shagieva G, Chaponnier C, Kopnin B, Kopnin P. Tumor promotion by γ and suppression by β non-muscle actin isoforms.
Oncotarget. 2015 Jun 10;6(16):14556-71.

Published customer image:
Mouse anti Human Actin gamma antibody, clone 2A3 used for the evaluation of γ-actin expression in A549 cells by immunofluorescence and western blotting.
Image caption:
γ-actin co-localizes with cofilin1 and regulates it A. LSM of A549 cells with down-regulated β- or γ-actins with β-actin (green), γ-actin (purple), cofilin1 (red) or DAPI (blue) immunofluorescent staining. Scale bars represent 10 μm. B. WB analysis of A549 cells with down-regulated β- or γ-actins. Graphs represent relative protein levels (Mean ± SD). C. Cofilin1 immunoprecipitation analysis of A549 cells with down-regulated β- or γ-actins. D. Cofilin1/γ-actin PLA analysis of A549 cells with down-regulated β- or γ-actins. Scale bar represents 10 μm. Graph represents relative fluorescence intensity (Mean ± SD).

From: Dugina V, Khromova N, Rybko V, Blizniukov O, Shagieva G, Chaponnier C, Kopnin B, Kopnin P. Tumor promotion by γ and suppression by β non-muscle actin isoforms.
Oncotarget. 2015 Jun 10;6(16):14556-71.

Published customer image:
Mouse anti Human Actin gamma antibody, clone 2A3 used for the evaluation of γ-actin expression in A549 cells by immunofluorescence and western blotting.
Image caption:
γ-actin co-localizes with PP1 and regulates it A. LSM of A549 cells with down-regulated β- or γ-actins with β-actin (green), γ-actin (purple), PP1α (red) or DAPI (blue) immunofluorescent staining. Scale bars represent 10 μm. B. WB analysis of A549 cells with down-regulated β- or γ-actins. Graphs represent relative PP1&alpha levels (Mean ± SD). C. PP1 immunoprecipitation analysis of A549 cells with down-regulated β- or γ-actins. D. PP1α/γ-actin (upper panel) and PP1α/β-actin PLA analysis of A549 cells with down-regulated β- or γ-actins. Scale bar represents 10 μm. E. Comparative quantification of PP1α-β-/γ-actin PLA dots. Graph represents relative fluorescence intensity (Mean ± SD).

From: Dugina V, Khromova N, Rybko V, Blizniukov O, Shagieva G, Chaponnier C, Kopnin B, Kopnin P. Tumor promotion by γ and suppression by β non-muscle actin isoforms.
Oncotarget. 2015 Jun 10;6(16):14556-71.